Inhibitor Resistant Genotyping PCR ReadyMixes enable genetic analysis directly from crude samples such as lysates, blood spots, plant tissues and clinical samples. Each is a ready-to-use 2X concentrated hot-start PCR mix containing additives which prevent inhibition of PCR or quenching of fluorescent signal by common PCR inhibitors.

  • PCR Inhibitor Resistant – Overcomes common inhibitors including polysaccharides, heme/hemoglobin, humic acid, melanin
  • Fast – Leads to assay results in as little as 33 minutes
  • Sensitive – Supports genotyping with low primer & probe concentration
  • Flexible – Keeps assembled reactions stable 3 days pre/post-PCR

Overcome PCR Troublemakers

Many sample types are rich in PCR inhibitors (Table 1). These inhibitors operate by shutting down PCR amplification at threshold concentrations, or by quenching fluorescent signal. Inhibitor Resistant Genotyping PCR ReadyMixes neutralize problems caused by inhibitors in crude lysates and other difficult samples, eliminating the need for expensive or time-consuming purification steps.

Many sample types are rich in PCR inhibitors

Comparison to Conventional Master Mixes

Inhibitor Resistant Genotyping PCR ReadyMixes can be used in the presence of common PCR inhibitors. The competitor’s system is completely shut down while Inhibitor Resistant Genotyping PCR ReadyMixes delivers accurate results (Figure 1).

Inhibitor Resistant Genotyping PCR ReadyMixes

In the presence of humic acid, Inhibitor Resistant Genotyping PCR Readymix outperforms Competitor I. Note the higher intensity and spread of SNPs analyzed for Readymix samples.

Extended Product Stability

Variability in PCR experiments is often caused by degradation of reagent performance with time and handling. The ReadyMix PCR reagents provide extended stability for storage and after reaction setup (Figure 2).

  • 3 days pre-PCR stability (assembled reactions)
  • 5 days post-PCR stability (cycled reactions)
  • 2 years shelf-life -20 °C
  • > 6 months shelf-life 8 °C
  • > 20 freeze-thaw cycles
Extended Product Stability

Compatible with Most Instruments

 

Inhibitor Resistant Genotyping PCR ReadyMix Compatible Instruments
PIR00 Bio-Rad CFX384™, Bio-Rad CFX96™, Bio-Rad MiniOpticon™, Bio-Rad/MJ Chromo4™, Bio-Rad/MJ Opticon 2, Bio-Rad/MJ Opticon®, Cepheid SmartCycler®, Eppendorf Mastercycler® ep realplex, Eppendorf Mastercycler® ep realplex2 s, Illumina Eco qPCR, Qiagen/Corbett Rotor-Gene® 3000, Qiagen/Corbett Rotor-Gene® 6000, Qiagen/Corbett Rotor-Gene® Q, Roche LightCycler™ 480
PIR01 Applied Biosystems 7500, Applied Biosystems 7500 Fast, Applied Biosystems ViiA 7, Stratagene Mx3000P®, Stratagene Mx3005P™, Stratagene Mx4000™
PIR02 Applied Biosystems 5700, Applied Biosystems 7000, Applied Biosystems 7300, Applied Biosystems 7700, Applied Biosystems 7900, Applied Biosystems 7900 HT Fast, Applied Biosystems 7900HT, Applied Biosystems StepOnePlus™, Applied Biosystems StepOne™

 

Materials

     

ReadyMix is a trademark of Sigma-Aldrich Co. LLC, TaqMan is a registered trademark of Roche Molecular Systems, Inc.  LightCycler is a registered Trademark of Roche. Applied Biosystems, StepOne, StepOnePlus, ViiA, and ROX are trademarks Life Technologies Corporation. Stratagene, MX3000P, MX3005P and MX4000 are trademarks of Agilent Technologies, Inc. Mastercycler is a trademark of Eppendorf. Rotor-Gene is a registered trademark of Qiagen GmbH.  SmartCycler is a trademark of Cepheid. CFX96, CFX384, iCycler iQ, iQ5, MyiQ, Opticon, MiniOpticon and Chromo4 are trademarks of Bio-Rad Laboratories. Refer to product technical bulletin for product licensing information.

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