Isopropanol Molecular Biology Reagent

Product No. I9516

CAS RN 67-63-0
EC 200-661-7
Synonyms: 2-Propanol, sec-Propyl alcohol, IPA, Isopropyl alcohol

Product Description

Isopropanol is a polar organic solvent that is widely used in chemistry, biochemistry and molecular biology. Industrial applications include its use in antifreezes, quick-drying oils, and quick-drying inks, and as a solvent for gums, shellac, and essential oils.1 Isopropanol has been utilized as a solvent in the synthesis of solid phase acylating agents.2

Protocols for the use of isopropanol in DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues have been reported.3 Isopropanol has been utilized in protein crystallization.4,5,6 The use of isopropanol to isolate retinoids from biological matrices for HPLC and HPLC-MS analysis has been reported.7,8 A mathematical analysis of isopropanol and other organic solvents to examine the selectivity of stationary phases and organic modifiers in reversephase HPLC systems has been published.9

Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Preparation Instructions

This product is miscible in water, alcohol, ether, and chloroform.1

Product Profile

Molecular Formula: C3H8O
Molecular Weight: 60.10
Boiling Point: 82.5 °C (760 torr)1
Density: 0.78505 g/ml (20 °C)

Specificity

This product is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

Materials

     

 References

  1. The Merck Index, 12th ed., Entry# 5227.
  2. Nicewonger, R. B., et al., Synthesis of a novel, recyclable, solid-phase acylating reagent. Bioorg. Med. Chem. Lett., 12(14), 1799-1802 (2002).
  3. Molecular Cloning: A Laboratory Manual, 3rd ed., Sambrook, J. and Russell, D.W., CSHL Press (Cold Spring Harbor, NY: 2001), pp. 1.151, 6.23- 6.30, 7.4-7.12, A8.27.
  4. Romao, M. J., et al., Subunit composition, crystallization and preliminary crystallographic studies of the Desulfovibrio gigas aldehyde oxidoreductase containing molybdenum and [2Fe-2S] centers. Eur. J. Biochem., 215(3), 729- 732 (1993).
  5. Knapp, S., et al., Crystallization and preliminary crystallographic analysis of an amylopullulanase from the hyperthermophilic archaeon Pyrococcus woesei. Proteins, 23(4), 595-597 (1995).
  6. Yang, F., et al., Crystal structure of cyanovirin-N, a potent HIV-inactivating protein, shows unexpected domain swapping. J. Mol. Biol., 288(3), 403-412 (1999).
  7. Schmidt, C. K., et al., Chromatographic analysis of endogenous retinoids in tissues and serum. Anal. Biochem., 315(1), 36-48 (2003).
  8. McCaffery, P., et al., Retinoid quantification by HPLC/MSn. J. Lipid Res., 43(7), 1143-1149 (2002).
  9. Du, C. M., et al., Characterizing the selectivity of stationary phases and organic modifiers in reversed-phase high-performance liquid chromatographic systems by a general salvation equation using gradient elution. J. Chromatogr. Sci., 38(11), 503-511 (2000).

 

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