Magnesium Chloride Solution for molecular biology

Product No. M1028

CAS RN 7786-30-3

Product Description

This product is a 1.00 ± 0.01 M solution, prepared in 18 megohm water and 0.2 µm filtered. It is designated as Molecular Biology grade and is suitable for molecular biology applications. This product has been analyzed for the presence of nucleases and proteases.

Magnesium chloride is a widely used reagent in chemistry and molecular biology as a source of magnesium ion. Magnesium has a variety of biological roles in enzymology, cell membrane and wall structural integrity, muscle cell physiology, and nucleic acid structure.1,2 Magnesium is an essential co-factor in many enzymes, including deoxyribonuclease (DNAse), the restriction enzymes EcoR I and EcoR V, and Ribonuclease H.3,4 Magnesium also stabilizes polymeric nucleic acids such as transfer RNA and ribozymes.5

Conditions for optimal use of MgCl2 in the polymerase chain reaction (PCR) have been investigated.6,7 The use of MgCl2 in the trypsin-mediated proteolysis of the mammalian α-ketoglutarate dehydrogenase complex has been reported.8 A protocol that includes MgCl2 for dideoxy-mediated sequencing reactions using bacteriophage T7 DNA polymerase has been published.9

Storage

Room Temperature

Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. 

Materials

     

 References

  1. Cowan, J. A., in The Biological Chemistry of Magnesium, Cowan, J. A., ed., VCH Publishers (New York: 1995), pp. 1-23.
  2. The Biological Chemistry of the Elements, Frausto da Silva, J. J. R., and Williams, R. J. P., Clarendon Press (Oxford, UK: 1991), pp. 243-267.
  3. Brooks, J. E., Properties and uses of restriction endonucleases. Meth. Enzymol., 152, 113-129 (1987).
  4. Black, C. B., and Cowan, J. A., in The Biological Chemistry of Magnesium, Cowan, J. A., ed., VCH Publishers (New York: 1995), pp. 137-157.
  5. Principles of Bioinorganic Chemistry, Lippard, S. J., and Berg, J. M., University Science Books (Mill Valley, CA: 1994), pp. 192-196.
  6. Harris, S., and Jones, D.B., Optimisation of the polymerase chain reaction. Br. J. Biomed. Sci., 54(3), 166-173 (1997).
  7. Henegariu, O., et al., Multiplex PCR: critical parameters and step-by-step protocol. Biotechniques, 23(3), 504-511 (1997).
  8. McCartney, R.G., et al., Subunit interactions in the mammalian alpha-ketoglutarate dehydrogenase complex. Evidence for direct association of the α-ketoglutarate dehydrogenase and dihydrolipoamide dehydrogenase components. J. Biol. Chem., 273(37), 24158-24164 (1998).
  9. Molecular Cloning: A Laboratory Manual, 3rd ed., Sambrook, J. and Russell, D.W., CSHL Press (Cold Spring Harbor, NY: 2001), pp. 12.32-12.37.

 

Related Links