Microcon® Centrifugal Filters

Microcon® Centrifugal FiltersMicrocon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes. With the low- binding Ultracel® membrane, Microcon® filters offer:

  • Typical recoveries of >95%, even for dilute solutions
  • Reverse spin to maximize recovery, even in the smallest samples
  • Convenient storage of filtrate or concentrated sample in standard microfuge tube.

The Microcon® DNA Fast Flow Filter isis optimized for the concentration and recovery of genomic DNA. The low non-specific binding characteristics of the membrane and the other device components, coupled with its medical-grade o-ring seal, allows the device to accommodate several wash steps with minimal sample loss. With its inverted recovery spin, the device can concentrate samples up to 100X (for greatest consistency and reproducibility, we recommend concentration factors of < 20X).

Applications Guidelines

Use the following table to choose the correct Microcon® device for your application.

  Microcon® Device
Application 10K 30K DNA Fast Flow
Peptide and growth factor concentration    
Protein concentration and desalting of columns eluates  
Protein concentration before electrophoresis or other assays  
Protein removal prior to HPLC  
Purification of macromolecular components found in tissue culture extracts and
cell lysates
 
Concentration of biological samples (antigens, antibodies, enzymes)    
Concentration and desalting of nucleic acids (single-or double-stranded)
Removal of labeled nucleotides
Removal of labeled amino acids
Removal of primers from amplified DNA  
Removal of linkers prior to cloning  

Specifications

Maximum initial sample volume 0.5 mL (500 μL)
Typical final concentrate volume  5–50 μL
Maximum relative centrifugal force
Microcon® 10K devices 14,000 × g
Microcon® 30K devices 14,000 × g
Microcon® DNA Fast Flow devices 500 × g
Active membrane area 0.32 cm2
Hold-up volume ≤ 10 μL

Ordering Information

Microcon® Centrifugal Filters
Product No. Description Pack Size Add to Cart
MRCF0R030 Microcon-30kDa Centrifugal Filter Unit with Ultracel-30 membrane 100
MRCF0R100 Microcon DNA Fast Flow Centrifugal Filter Unit with Ultracel membrane 100
MRCF0R100ET Microcon DNA Fast Flow PCR Grade with Ultracel membrane, dual cycle EtO treated 20
MRCPRT010 Microcon-10kDa Centrifugal Filter Unit with Ultracel-10 membrane 100