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OligoArchitect™ Consultative

 

Request Design


For more complex and demanding applications, utilize our consultative service to ensure the success of your assay. With personal consultation from our expert, technical support scientists, your request, including all sequences and data analysis, will be MIQE compliant and returned to you within 24-48 hours. If required, you will also receive follow-up assay optimization, data analysis assistance, and troubleshooting support.

 

Specifications
Design Modules PCR and qPCR Applications
  • SYBR®Green I Primers
  • Dual-Labeled Probes1
  • Molecular Beacons2
  • LightCycler® Probes3
  • Scorpions® Probes
  • Traditional PCR
  • SNP detection
  • Allele discrimination
  • Pathogen detection
  • Multiplexing
  • Viral load quantification
  • Gene expression analysis
  • Gene copy determination
  • Endpoint genotyping
  • Methylation analysis
  • High-resolution melting analysis
  • Transcript detection across exon junctions
  • Haplotyping
  • Northern blotting
  • Southern blotting
Other Applications
  • FISH (fluorescence in situ hybridization)
 
Modifications
  • Add LNA® to Dual-Labeled Probes, Molecular Beacons, LightCycler Probes, and Scorpions Probes

1 Also known as hydrolysis probes, which are used in the 5’ nuclease assay
2 Also known as hybridization probes
3 Also known as dual-hybridization probes

Sigma’s consultative service uses Beacon Designer™ from Premier Biosoft International. Blastn and mfold are used to determine assay specificity.

Materials