SnapFast™ Cloning & Expression Vectors Selection Charts

With over 1600 vectors to choose from, your options may seem endless. For help choosing the right vector for your research needs, consult the tables below.

Bacterial vector options with FLAG and 3xFLAG tags

Product Number FLAG tag OmpA secretion tag Ampicillin Selection Marker EKT cleavage tag Promoter
N-terminal C-terminal Inducible TAC T7 Lac
OGS632 x     x x x x  
OGS633 x   x x x x x  
OGS634   x   x x x x  
OGS635   x x x x x x  
OGS636 x     x x x   x

Mammalian vector options with FLAG and 3xFLAG tags

Product Number FLAG tag 3X FLAG tag C-myc tag EKT cleavage tag PPT secretion tag Ampicillin Selection Marker (Bacterial) Neomycin Selection Marker (Mammalian) Promoter
N-terminal C-terminal N-terminal C-terminal C-terminal Constitutive CMV
OGS618 x         x   x   x x
OGS619   x       x   x   x x
OGS620     x     x   x   x x
OGS621     x     x x x   x x
OGS622     x   x x x x   x x
OGS623     x   x x   x   x x
OGS624 x         x x x x x x
OGS625 x         x   x x x x
OGS626     x     x x x x x x
OGS627     x     x   x x x x
OGS628       x   x x x x x x
OGS629       x   x   x x x x
OGS630     x   x x x x x x x
OGS631     x   x x   x x x x

Vector options for single functional tags for use in yeast cells

Yeast Plasmids Encoding Single Functional Tags

N Terminal Tag Plasmids are in Blue
C Terminal Tag Plasmids are in Red

Vector options for dual functional tags for use in yeast cells

Yeast Plasmids Encoding Dual Functional Tags

N Terminal Tag Plasmids are in Blue
C Terminal Tag Plasmids are in Red

Vector options for fluorescent reporters

Fluorescence Reporter Gene Plasmids

  GFP
(DaGFP)
YFP
(KrYFP)
CFP
(FrCFP)
Single Gene Vectors Choose a promoter to drive the reporter gene CMV Promoter OGS242 OGS510 OGS509
Minimal CMV Promoter OGS573 OGS574 OGS575
Systems for you to insert your own promoter With polyA upstream of MCS - to minimise background Prom-MCS-pAa OGS241 OGS614 OGS615
MinProm-pAb OGS251
CMVe-pAc OGS257
Without upstream pA - better for cloning into retrovirus or lentivirus Prom MCS (no pA)a OGS347
MinProm (no pA)b OGS362
CMVe (no pA)c OGS368
Double Gene Vectors (place your gene of interest into the MCS, under CMV promoter control) Chose a promoter to drive the reporter gene RSV OGS243 OGS516 OGS515
Ubiquitin   OGS513 OGS512
PGK OGS390 OGS611 OGS610
IRES (Internal Ribosome Entry Site) FMDV OGS289 OGS521 OGS522
EMCV OGS315 OGS518 OGS519
Reporter Fusion Protein Contructs See separate table

NOTES:

(a)PromMCS plasmids have no promoter driving the reporter gene, but they have several unique restriction sites that can be used to insert the promoter of your choice.

(b)MinProm plasmids contain the minimal HSV TK promoter driving the reporter gene, with an upstream MCS that can be used to introduce specificity-defining sequences (such as transcription factor binding sites) of your choice

(c)CMVe plasmids contain the CMV enhancer upstream of the MCS, for your to insert your promoter of choice. The CMV enhancer should augment its activity.

Vector options for fusion reporters

Mammalian Reporter Fusion Protein Plasmids

N Terminal Tag Plasmids are in Blue
C Terminal Tag Plasmids are in Red

Vector options for the Luciferase reporter

Luciferase Reporter Gene Plasmids

  Firefly Luc
(cytosolic)
Renilla Luc (cytosolic) iLumena Luc (secreted)
Single Gene Vectors Choose a promoter to drive the reporter gene CMV Promoter OGS102 OGS103 OGS511
OGS1378 (puro)
Minimal CMV Promoter OGS570 OGS571 OGS578
Other Promoters Chicken beta actin OGS598 CAG OGS508  
Systems for you to insert your own promoter With polyA upstream of MCS - to minimise background Prom-MCS-pAa OGS236 OGS237 OGS616
MinProm-pAb OGS246 OGS247  
CMVe-pAc OGS252 OGS253  
Without upstream pA - better for cloning into retrovirus or lentivirus Prom MCS (no pA)a OGS369 OGS370  
MinProm (no pA)b OGS357 OGS358  
CMVe (no pA)c OGS363 OGS364  
  Chose a promoter to drive the reporter gene CMV OGS608    
Double Gene Vectors (place your gene of interest into the MCS, under CMV promoter control) RSV OGS15 OGS16 OGS517
Ubiquitin OGS155 OGS156 OGS514
PGK OGS388 OGS389 OGS609
IRES (Internal Ribosome Entry Site) FMDV OGS285 OGS286 OGS523
EMCV OGS295
OGS602 (puro)
OGS296 OGS520
Reporter Fusion Protein Contructs See separate table

NOTES:

(a)PromMCS plasmids have no promoter driving the reporter gene, but they have several unique restriction sites that can be used to insert the promoter of your choice.

(b)MinProm plasmids contain the minimal HSV TK promoter driving the reporter gene, with an upstream MCS that can be used to introduce specificity-defining sequences (such as transcription factor binding sites) of your choice

(c)CMVe plasmids contain the CMV enhancer upstream of the MCS, for your to insert your promoter of choice. The CMV enhancer should augment its activity.

Vector options for Colorimetric reporters

Colorimetric Reporter Gene Plasmids

  Alkaline phosphatase SEAP (secreted) Beta galactosidase (cytosolic) CAT Chloramphenicol Acetyl Transferase (cytosolic)
Single Gene Vectors Choose a promoter to drive the reporter gene CMV Promoter OGS105 OGS101
OGS594 (+Puro)
OGS104
Minimal CMV Promoter OGS577 OGS572 OGS576
Other Promoters   CAG OGS506 (+Puro)  
Systems for you to insert your own promoter With polyA upstream of MCS - to minimise background Prom-MCS-pAa OGS238 OGS239 OGS240
MinProm-pAb OGS248 OGS249 OGS250
CMVe-pAc OGS254 OGS255 OGS256
Without upstream pA - better for cloning into retrovirus or lentivirus Prom MCS (no pA)a OGS371 OGS372 OGS373
MinProm (no pA)b OGS359 OGS360 OGS361
CMVe (no pA)c OGS365 OGS366 OGS367
Double Gene Vectors (place your gene of interest into the MCS, under CMV promoter control) Chose a promoter to drive the reporter gene RSV OGS17 OGS18 OGS19
Ubiquitin OGS157 OGS158 OGS159
PGK OGS392 OGS391 OGS393
IRES (Internal Ribosome Entry Site) FMDV OGS287    
EMCV      
Reporter Fusion Protein Contructs See separate table

NOTES:

(a)PromMCS plasmids have no promoter driving the reporter gene, but they have several unique restriction sites that can be used to insert the promoter of your choice.

(b)MinProm plasmids contain the minimal HSV TK promoter driving the reporter gene, with an upstream MCS that can be used to introduce specificity-defining sequences (such as transcription factor binding sites) of your choice

(c)CMVe plasmids contain the CMV enhancer upstream of the MCS, for your to insert your promoter of choice. The CMV enhancer should augment its activity.

Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.