Protease Inhibitors – Don't Lose Your Proteins During Sample Prep

Protect Your Proteins

Protease inhibitors are valuable and useful reagents for researchers who want to inhibit general degradation of proteins in tissue or cell extracts by endogenous proteases. During isolation and characterization, proteases may pose a threat to the fate of a protein.

Four main classes of proteolytic enzymes are routinely utilized to describe proteases:

  • Serine Protease Class includes trypsin, chymotrypsin and elastase.
  • Cysteine Protease Class includes papain, calpain and lysosomal cathepsins.
  • Aspartic Protease Class includes pepsin and rennin
  • Metallo-protease Class include thermolysin and carboxypeptidase A

During isolation and characterization, one or all four classes of proteases may pose a threat to the fate of a protein. Broad spectrum protease inhibitors and cocktails (mixtures) have been developed to protect the integrity of proteins against multiple classes of proteases.

Protease inhibitor cocktails have been developed to safeguard the integrity of proteins during extraction and purification, to protect your proteins during sample prep. Sigma-Aldrich offers the broadest range of well-established individual protease inhibitors and protease inhibitor cocktails on the market. In addition to the Sigma-Aldrich line of protease inhibitor cocktails, our product offerings now include Roche’s cOmplete protease inhibitor cocktail tablets.

Sigma-Aldrich offers the broadest range of well-established individual protease inhibitors and protease inhibitor cocktails that are optimized to maintain and preserve protein functionality following cell lysis.

  • Product offerings now include Roche’s cOmplete protease inhibitor cocktail tablets
  • Available in powder, solution, or tablet form
  • Inhibits proteases in almost any sample type (tissue or cell)
  • Specific formulations optimized for particular applications

View our individual protease inhibitors at
sigma-aldrich.com/proteaseinhibitors

View our protease inhibitor cocktails at
sigma-aldrich.com/proteaseinhibitorscocktails

Protease Inhibitor Cocktails

Cat. No. Sample Type Specificity of Inhibition Additional information Package sizes
I3911 General Serine, cysteine, aspartic, and metalloproteases Lyophilized powder that is water-soluble 1 bottle prepares 100 mL of cocktail solution
4693116001 General Serine, cysteine, and metalloproteases Standard-Roche cOmplete 20 tablets
11697498001 General Serine, cysteine, and metalloproteases Standard-Roche cOmplete 20 tablets
11836145001 General Serine, cysteine, and metalloproteases Standard-Roche cOmplete 3 × 20 tablets
4693124001 General Serine, cysteine, and metalloproteases Mini-Roche cOmplete 30 tablets
11836153001 General Serine, cysteine, and metalloproteases Mini-Roche cOmplete 25 tablets
5892988001 General Serine, cysteine, metallo-, and aspartic proteases ULTRA-Roche cOmplete 2 × 10 tablets
6538304001 General Serine, cysteine, metallo-, and aspartic proteases ULTRA-Roche cOmplete 6 × 10 tablets
5892970001 General Serine, cysteine, metallo-, and aspartic proteases ULTRA, Mini-Roche cOmplete 30 tablets
P2714 General Serine, cysteine, aspartic and metalloproteases Optimized and tested for general use 1 bottle prepares 100 mL of cocktail solution
S8820 General Serine, cysteine, aspartic and metalloproteases Optimized and tested for general use 20 tablets
P8465 Bacterial Serine, cysteine, aspartic, metalloproteases and aminopeptidases Various E. coli strains 5 mL, 25mL
P8340 Mammalian serine, cysteine, aspartic proteases, and aminopeptidases Use at 1:100 for undiluted blood samples, liver tissue extracts and CHO cell lysates 1mL, 5mL
I3786 Mammalian serine, cysteine, aspartic proteases, and aminopeptidases Solution in DMSO • 1 mL • 5 mL
MSSAFE Mammalian Protease/Phosphotase Inhibitor Lyophilized powder that is water-soluble 1VL
P9599 Plant Serine, cysteine, aspartic, metalloproteases and aminopeptidases Tested on extracts from kidney bean, pea, wheat, tobacco and Arabidopsis 1mL, 5mL
P8215 Yeast Serine, cysteine, aspartic and metalloproteases Saccharomyces cerevisiae cells 1mL, 5mL
4693132001 Histidine-tagged proteins Serine, cysteine EDTA-free-Roche cOmplete 20 tablets
11873580001 Histidine-tagged proteins Serine and cysteine proteases EDTA-free-Roche cOmplete 20 tablets
5056489001 Histidine-tagged proteins Serine and cysteine proteases EDTA-free-Roche cOmplete 3 × 20 tablets
04693159001 Histidine-tagged proteins Serine and cysteine proteases Mini, EDTA-free-Roche cOmplete 30 tablets
11836170001 Histidine-tagged proteins Serine and cysteine proteases Mini, EDTA-free-Roche cOmplete 25 tablets, glass vials
5892953001 Histidine-tagged proteins Serine, cysteine, and aspartic proteases ULTRA, EDTA-free-Roche cOmplete 2 × 10 tablets
6538282001 Histidine-tagged proteins Serine, cysteine, and aspartic proteases ULTRA, EDTA-free-Roche cOmplete 6 × 10 tablets
5892791001 Histidine-tagged proteins Serine, cysteine, and aspartic proteases ULTRA, Mini, EDTA-free-Roche cOmplete 30 tablets
P8849 Histidine-tagged proteins Serine, cysteine, aspartic and thermolysin-like proteases and aminopeptidases E. coli cells; baculovirus infected Spodoptera frugiperda pupal ovary cells 1mL, 5mL
S8830 Histidine-tagged proteins Serine, cysteine, aspartic and metalloproteases Optimized and tested for use with HIS-Select media 20 tablets
P1860 Tissue Culture Media Serine cysteine, aspartic proteases and aminopeptidases After 48 hours in medium, add fresh inhibitors in fresh medium 1 mL

 

Find individual protease inhibitors at
sigma-aldrich.com/proteaseinhibitors