Reporters & Quenchers – Dual-Labeled DNA Probes

Spectral properties of fluorophores

The table below details the most commonly used dyes for the fluorescent probes, together with their excitation and emission data. Please enquire for any other labels.

Quenchers Maximum absorbance (nm) Maximum emission (nm)
BHQ-1 Dark Quencher 535 None
BHQ-2 Dark Quencher 579 None
DABCYL Dark Quencher 453 None
DABCYL-dT Dark Quencher 453 None
TAMRA 550 576
Labels Maximum absorbance (nm) Maximum emission (nm)
6-FAM 495 517
Cy3 550 570
Cy5 650 667
Cy5.5 675 694
Fluorescein 495 520
HEX 537 553
JOE 520 548
LightCycler Red 610 590 610
LightCycler Red 640 625 640
LightCycler Red 670 650 670
LightCycler Red 705 680 705
Oregon Green 488 495 521
Oregon Green 500 499 519
Oregon Green 514 506 526
Rhodamine 564 603
6-CarboxyRhodamine 6G 524 557
Rhodamine Green 504 532
Rhodamine Red 570 590
ROX 581 607
TAMRA 550 576
TET 521 538
Texas Red 589 610