High Quality Products for Nucleic Acid Research

Deuterated NTPs Available

“Over the course of several years now, my group hasconsistently been happy with the quality of the NTPsand services provided by ISOTEC. We are particularlypleased by the fact that the NTPs are often availablein stock and can be ordered in large quantities atshort notice. We routinely obtain very high yieldswhen using their NTPs and the customer serviceis exceptional.”

– Dr. Hashim M. Al-Hashimi
   Robert L. Kuczkowski Professor of Chemistry
   University of Michigan

Stable isotope labeled nucleic acids play a significant rolein studying the links between the structure and dynamicsof the overall global conformation of RNA and DNA molecules. These labeled nucleic acids help to improve NMR methodologies by enhancing experimental sensitivity and spectral resolution, as well as facilitating the measurement of local and long-range distance restraints.1-3 Uniformly enriched 13C and/or 15N nucleic acids have been the most prominent labeling patterns; however, deuterated nucleotides are becoming more important as the size of the studied molecules is becoming larger than 40 nucleotides long.

With the increased size, the resonance line widths andsignal crowding become large obstacles that can besignificantly reduced by replacing the hydrogen atomswith deuterium.4 Deuterated nucleotides can be used toenzymatically synthesize RNA and DNA molecules allowing for the simplification of coupling patterns by reducing line widths, removing nonessential resonances, and increasing NOE intensities.5,6

Each NTP is manufactured and packaged with the following standards

  • Minimum of 95% chemical purity with theremaining portion consisting of the corresponding NMPsand NDPs
  • Minimum 98 atom % isotopic purity
  • Supplied as the sodium salt, in 100 mM solution with5 mM Tris buffer
  • Convenient 1 mg, 10 mg, and 25 mg quantities
  • To verify the highest standards, each product is analyzedby multiple analytical methods including
    • Structure verification by 1H and 13C NMR
    • Chemical purity by HPLC
chromatogram of ATP-13C1015N5

This chromatogram of ATP-13C1015N5, demonstrates the chemical purity of the compound(96.5%) in relation to AMP (2.4%) and ADP (1.1%).

Materials

     

  1. Dayie, K.T.; Resolution Enhanced Homonuclear Carbon Dicoupled TripleResonance Experiments for Unambiguous RNA Structural Characterization.J. Biomol. NMR. 2005, 32, 129-139
  2. Dethoff, E.A.; Hansen, A.L.; Musselman, C.; Watt, E.D.; Andricioaei, I.; Al-Hashimi, H.; Characterizing Complex Dynamics in the TransactivationResponse Element Apical Loop and Motional Correlations with the Bulgeby NMR, Molecular Dynamics, and Mutagenesis. Biophys. J. 2008, 95, 3906
  3. Bailor, M.H.; Sun, X.; Al-Hashimi, H.; Topology Links RNA Secondary Structurewith Global Conformation, Dynamics, and Adaptation. Science. 2010, 327,202-206
  4. Dayie, K.T.; Key Labeling Technologies to Tackle Sizeable Problems in RNAStructural Biology. Int. J. Mol. Sci. 2008, 9, 1214-1240
  5. Chen, B.; Jamieson, E.R.; Tullius, T.D.; A General Synthesis of SpecificallyDeuterated Nucleotides for Studies of DNA and RNA. Bioorg. & Med. Chem.Lett. 2002, 12, 3093-3096
  6. Lu, K.; Miyazaki, Y.; Summers, M.F.; Isotope Labeling Strategies for NMRStudies of RNA. J. Biomol. NMR. 2010, 46, 113-125

 

Related Links