ISOGRO® Complex Growth Media

Visit our ISOGRO Complex Growth Media page to find detailed information on products and protocols for using ISOGRO as a suppplement and as a stand alone media.

Performance Surpasses the Competition as a Stand-alone Media

  • Improve recombinant protein yields up to 80% compared to commercially available complex growth media “B”.
  • Substantially increase recombinant protein expression levels using ISOGRO versus M9 media.
  • Save time by using ISOGRO growth media to shorten production time.
  • Express recalcitrant proteins to obtain high resolution NMR structural data.

For optimal results as a stand-alone media, incorporate 10g of ISOGRO per Liter of culture.

The final yield of purified recombinant protein derived from each liter of culture

Figure 1. The final yield of purified recombinant protein derived from each liter of culture. Acknowledgement: Data provided by Dr. Ross Overman and Dr. Kevin Embry, AstraZeneca, U.K.

 

A 39 μM sample of p38 alpha was produced from 50 mL of culture as seen below:

SDS-PAGE of p38 growth

Figure 2. SDS-PAGE of p38 growth. Acknowledgement: Data provided by Dr. Jeffrey W. Peng, Dept. of Chem/Biochemistry, Univ. of Notre Dame, Notre Dame, Indiana

Supplement M9 Media with ISOGRO for Enhanced Protein Expression

  • Decrease lag time by as much as 60%.
  • Maximize OD and recombinant protein expression.
  • Supplement M9 media with as little as 1 gram of ISOGRO per Liter of culture and improve the production of difficult to express proteins in E. coli.
  • As a standard quality control measure, the suitability of each batch of ISOGRO as a culture medium is determined by comparison with an LB growth curve.
Cardiac troponin cTnC(1-89) in pLysS

Figure 3. Cardiac troponin cTnC(1-89) in pLysS. Cells grown at 37 °C in shaker flasks. Red curve is ISOGRO supplemented minimal media and black curve is minimal media alone.

SDS-PAGE cTnC(1-89) cell lysates

Figure 4. SDS-PAGE cTnC(1-89) cell lysates. Data provided by Dr. Paul R. Rosevear The Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati Medical Center, Cincinnati, OH.

ISOGRO Analytical Information

(Approximate values – there may be some variation between batches)

Composition:

Salts 30%
Water 3%
Glucose 2%
Amino acids/Peptides 65%

Amino Acid Analysis:

Ala 13%   Ile 4%   Pro 4%
Arg 3%   Leu 8%   Ser 4%
Asp 14%   Lys 6%   Thr 5%
Glu 10%   Met 3%   Tyr 3%
Gly 12%   Phe 4%   Val 6%
His 1%            

Materials

     
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