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Acid Fast Stain

Reagent Preparation

Malachite Green solution contains both counterstain and differentiator and is performed within the same step.


  1. Deparaffinize tissue sections and hydrate to deionized water.
  2. Stain in Carbol-Fuchsin solution for 30-45 minutes.
  3. Wash in deionzed water to remove excess stain.
  4. Differentiate and counterstain in Malachite Green solution for 2 minutes.
  5. Rinse in tap water and let air dry.
  6. Do not dehydrate slides through graded alcohols after staining.
  7. Dip slides individually in xylene and coverslip.


Acid fast bacteria – Red
Background – Green to turquoise

Acid Fast Stain


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