Classic Plant Media – Plant Tissue Culture Protocol

Product No. Description
C1416 Chu (N6) basal salt mixture
D6162 DKW/Juglans basal salt mixture
G5768 Gamborg's B-5 basal salt mixture
G5893 Gamborg's B-5 basal salt mixture with minimal organics
H2395 Hoagland's No. 2 basal salt mixture
M6774 McCown's woody plant basal salt mixture
M5524 Murashige and Skoog basal salt mixture (MS)
Q3376 Quoirin and Lepoivre basal salt mixture
S6765 Schenk and Hildebrandt basal salt mixture
W0876 White's basal salt mixture

 

Component (mg/L) C1416 D6162 G5768 G5893 H2395 M6774 M5524 Q3376 S6765 W0876
Aluminum chloride • 6H2O                    
Ammonium nitrate   1416.0       400.0 1650.0 400.0    
Ammonium phosphate monobasic         115.03       300.0  
Ammonium sulfate 463.0   134.0 134.0            
Boric acid 1.6 4.8 3.0 3.0 2.86 6.2 6.2 6.2 5.0 1.5
Calcium chloride anhydrous 125.33 112.5 113.24 113.24   72.5 332.2   151.0  
Calcium nitrate   1367.0     656.4 386.0   833.77   200.0
Calcium phosphate tribasic                    
Cobalt chloride • 6H2O     0.025 0.025     0.025 0.025 0.1  
Cupric sulfate • 5H2O   0.25 0.025 0.025 0.08 0.25 0.025 0.025 0.2  
Na2-EDTA 37.25 45.4 37.3 37.25   37.3 37.26 37.3 20.0  
ZnNa2-EDTA                    
Ferric chloride • 6H2O                    
Ferric citrate                    
Ferric sulfate                   2.5
Ferric tartrate • 2H2O         5.32          
Ferrous sulfate • 7H2O 27.85 33.8 27.8 27.85   27.8 27.8 27.8 15.0  
Magnesium sulfate 90.37 361.49 122.09 122.09 240.76 180.7 180.7 175.79 195.4 360.0
Manganese chloride • 4H2O         1.81          
Manganese sulfate • H2O 3.33 33.5 10.0 10.0   22.3 16.9 0.76 10.0 5.04
Molybdenum trioxide         0.016          
Molybdic acid (sodium salt) • 2H2O   0.39 0.25 0.25   0.25 0.25 0.25 0.1  
Nickel chloride • 6H2O                    
Nickel sulfate • 6H2O   0.005                
Potassium chloride                   65.0
Potassium iodide 0.8   0.75 0.75     0.83 0.08 1.0 0.75
Potassium nitrate 2830.0   2500.0 2500.0 606.6   1900.0 1800.0 2500.0 80.0
Potassium phosphate monobasic 400.0 265.0       170.0 170.0 270.0    
Potassium sulfate   1559.0       990.0        
Sodium nitrate                    
Sodium phosphate monobasic     130.5 130.5           16.5
Sodium sulfate                   200.0
Zinc nitrate • 6H2O   17.0                
Zinc sulfate • 7H2O 1.5   2.0 2.0 0.22 8.6 8.6 8.6 1.0 2.67
 
Organics (mg/L) C1416 D6162 G5768 G5893 H2395 M6774 M5524 Q3376 S6765 W0876
Activated charcoal                    
Adenine hemisulfate                    
Agar                    
6-Benzylaminopurine (BA)                    
D-Biotin                    
6gamma-gamma-
Dimethylallylaminopurine
                   
Glycine (free base)                    
Indole-3-acetic acid                    
Indole-3-butyric acid                    
Kinetin                    
MES (free acid)                    
myo-Inositol       100.0            
alpha-Naphthaleneacetic acid                    
Nictotinic acid (free acid)       1.0            
Peptone                    
Pyridoxine • HCl       1.0            
Sucrose                    
Thiamine • HCl       10.0            
 
  C1416 D6162 G5768 G5893 H2395 M6774 M5524 Q3376 S6765 W0876
Grams of powder to prepare 1 L 4.0 5.2 3.1 3.2 1.6 2.3 4.3 3.6 3.2 0.934
pH 1 0.5 at room temperature 4.1 4.1 4.0 4.0 4.7 3.9 3.9 3.9 4.2 4.7