Gomori’s Trichrome Stain

Reagent Preparation

Prepare working Weigert’s Iron Hematoxylin solution (Catalog No. HT1079) by adding equal amounts of Part A and Part B.

Procedure

  1. Deparaffinize tissue sections and hydrate to deionized water.
  2. Mordant in preheated Bouin’s solution (Catalog No. HT10-1) at 56 °C for 15 minutes.
  3. Wash in running tap water until yellow color is removed.
  4. Stain in working Weigert’s Iron Hematoxylin solution for 5 minutes.
  5. Wash in running tap water for 5 minutes.
  6. Stain in Trichrome Stain (LG or AB) solution for 5 minutes.
  7. Place in 0.5% Acetic Acid for 1 minute.
  8. Rinse slide in water and dehydrate in alcohol.
  9. Clear in xylene and mount.

Results

Nuclei – Black
Muscle fibers – Red
Collagen – (AB) Blue
                (LG) Green

Reagents

     
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