Mucicarmine Stain

Reagent Preparation

Prepare working Mucicarmine solution by diluting Mucicarmine Stock solution 1:4 with tap water (e.g. 10 mL Mucicarmine Stock solution plus 30 mL tap water. Working solution is stable for 2-3 days when stored in refrigerator. Prepare working Weigert’s Iron Hematoxylin solution by adding equal amounts of Part A and Part B.

Procedure

  1. Deparaffinize tissue sections and hydrate to deionized water.
  2. Stain in working Weigert’s Iron Hematoxylin solution for 5 minutes.
  3. Wash in running tap water for 5 minutes.
  4. Stain in Working Mucicarmine solution for 30 minutes or longer at room temperature.
  5. Rinse in deionized water.
  6. Stain in Tartrazine solution for 1-5 seconds.
  7. Rinse slides, dehydrated through alcohol, clear in xylene and mount.

Results

Nuclei – Blue
Mucin – Deep rose to red
Capsule of Cryptococci – Deep rose to red
Other tissue elements – Yellow

Mucicarmine Stain

Reagents

     
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