Papanicolaou Stain–Gill

Reagent Preparation

Mix one part Scott’s Tap Water Substitute with 9 parts deionized water (i.e. one bottle with 900 mL deionized water).

Procedure

  1. Fix slides in 95% alcohol for 15 minutes.
  2. Rinse in tap water.
  3. Stain in Gill’s Hematoxylin No. 2 solution for 1 to 3 minutes.
  4. Rinse in tap water.
  5. Dip slides 10 times in working Scott’s Tap Water Substitute.
  6. Rinse in tap water.
  7. Dip slides 10 times in 95% alcohol.
  8. Stain in OG-6 Solution for 1.5 minutes.
  9. Dip slides 10 times in 95% alcohol.
  10. Stain in EA-50 solution for 2.5 minutes.
  11. Dehydrate in 2 changes of 95% alcohol.
  12. Dehydrate in 100% alcohol for 1 minute.
  13. Clear in 2 changes of xylene and coverslip with mounting media.

Results

Nuclei are stained blue while cytoplasm displays varying shades of blue, orange, pink, and red.

Papanicolaou Stain-Gill

Reagents

     
Related Links