RNAstable® LD Protocol

Product No. 53201-013

Product Description

RNAstable LD is a unique storage medium that preserves RNA samples at room temperature. RNAstable LD enables you to store RNA samples for months. Using RNAstable LD, you can completely recover your RNA samples by simple rehydration - just add water. Rehydrated samples are ready for immediate use, without the need for further purification. 

Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Storage/Stability

RNAstable LD should be stored in their original unopened packaging ay 2-8°C until ready for use.

Procedures

Notes on Sample Preparation

Types of RNA. Suitable types of RNA that can be stored in RNAstable LD include purified total RNA from cells or tissues, and poly(A) mRNA.

Purification Techniques.  Most standard molecular biology techniques and/or commercially available kits are compatible with RNAstable LD storage. Purified RNA that is RNase-free should be resuspended in DEPC- treated water prior to application into RNAstable LD.

Determining yield. The concentration of the RNA sample should be determined prior to sample application into RNAstable LD. Although not essential, applying a known amount of RNA into RNAstable LD for storage can facilitate sample retrieval and subsequent applications. For optimal protection in RNAstable LD, add 20 ul RNAstable LD to 10 pg – 100 ug RNA sample directly into the tube or well. 

Sample Drying and Storage

  1. Determine the amount of purified RNA in the sample and calculate the amount to be applied into RNAstable LD wells or tubes.  RNA should be resuspended in DEPC-treated water prior to application on RNAstable LD.
  2. Gently add up to 20 μl of the RNAstable LD directly to 10 pg – 100 ug RNA sample in the tube or well. 
  3. Mix the sample thoroughly with gentle pipetting (avoid forming air bubbles).
  4. Dry the uncovered sample. For convenient air-drying of volumes ≤20 μl, leave the tube or 96-well plate open overnight in laminar flow hood.  For volumes ≥20 μl , or for accelerated drying of all, use a vacuum concentrator, without heat (see Table 1 below for drying times).

Table 1 – drying times with a vacuum concentrator

Sample Volume (μl) Drying Times (hrs)
Tubes
10–20 0.5
20–30 1.0
30–100 1.5
  1. Store samples with a desiccant packet in the original pouch and heat-sealed. Alternatively, store dried samples in a dry storage cabinet at room temperature. RNAstable LD is sensitive to temperature and humidity, therefore it is critical to maintain stored samples at room temperature (15-25°C) in an environment protected from moisture. The recommended humidity level is ≤ 30% relative humidity.

Sample Recovery

RNA stored in RNAstable LD can be recovered by the addition of water or aqueous buffer. Samples are ready for downstream applications without the need for further purification.

  1. Add 10-100 μl of DEPC-treated water or aqueous buffer directly to the dried sample in RNAstable LD.
  2. Incubate at room temperature for 15 min to allow complete rehydration.  
  3. Mix the sample by gently pipetting up and down. Avoid forming bubbles. The rehydrated sample is now ready for use directly in downstream applications.
  4. Store unused rehydrated samples at 4°C or room temperature for up to 3 days. Samples can also be stored frozen (-20°C or -80°C) for longer time periods. Rehydrated samples in water containing RNAstable LD can be re-dried without loss of efficient sample stabilization. Repeating this process more than 3 times is not recommended.

Materials

     
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