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BCIP Protocol

Product No. BCIPP-RO

Protocol

BCIP: Suitable solvents and storage

The recommended solvent will depend on the type of BCIP (5-bromo-4-chloro-3-indolyl-phosphate) salt you are using:

The disodium salt of BCIP (Cat.No. 1 017 373) is soluble in water at a concentration of 20 mg/ml. It is completely insoluble in organic solvents like dimethylformamide, DMSO, toluene, or ethanol. The solution in water is stable for only 6-8 hours at +2 to +8 °C protected from light. The disodium salt is also soluble in diethanolamine buffer, pH 9.8 but will decompose after 4 days.

The toluidine salt of BCIP (Cat.No. 760 994 [250 mg]; 1 585 002 [1g]) is soluble in dimethylformamide (DMF) at a concentration of 20 mg/ml and almost insoluble in water. The solution in DMF can be stored 1-2 days at +2 to +8 °C and may be further diluted with water. The compound is most probably also soluble in DMSO and toluene, and in alkaline aqueous solvents at pH 10-12 accompanied by precipitation of p-toluidine which can be removed by filtration or extraction.

BCIP: Use in combination with DIG system

When using the DIG system, the toluidine salt of BCIP is preferred over the disodium salt due to its higher stability.

Please note, that the following ready-to-use solutions of BCIP are also available from Roche Applied Science:

BCIP Solution, 3 mL, Cat No. 11383221001
NBT/BCIP Stock Solution, 8 mL, Cat No. 11681451001
BM Purple, 100 ml, Cat No. 11442074001

Materials

     
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