Glutamate Dehydrogenase (GlDH) Protocol

Product No. 10197734001

Protocol

Formulation of Glutamate Dehydrogenase

Glutamate Dehydrogenase is a component of cofactor recycling systems for NAD(P) and NAD(P)H. The formulation contains buffer substances, BSA as stabilizer and ADP as activator; please note that ADP can produce an adsorption peak at 260 nm. Less than <0.005% contaminants, such as ADH, LDH, and MDH each, may be present. For additional additives and contaminants, please refer to the Certificate of Analysis. Less than <0.005% contaminants, such as ADH, LDH, and MDH each, may be present.

For best results, dissolve the lyophilizate in redistilled water.

Stability of Glutamate Dehydrogenase

  1. Lyophilized material: Stable at +2 to +4 °C, when stored dry. A decrease in activity of approx. 10% can occur within one month, when stored dry at room temperature (+15 to +25 °C).
  2. Solution: Dissolving contents of the vial (3,000 U) in 2 ml redistilled water results in a solution with a pH of approx. 8. GIDH is very unstable at pH <5 or >8.

A 10% decrease in activity can occur within 7 days when stored at +2 to +8 °C, or within one day when stored at room temperature (+15 to +25 °C). There is no significant decrease in activity, when this solution is frozen or lyophilized.

Materials

     
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