Taq DNA Polymerase, GMP Grade Protocol

Product No. 03734927001

Protocol

PCR OPTIMIZATION

The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome. Template purity and quality are also critical to PCR success. Sequence and primer concentrations also determine overall assay quality. Nucleotides are vital components in amplification reactions and purity and concentration of these reagents significantly influences PCR results. Most thermostable DNA polymerases require divalent cations to function (in most cases Mg2+, and for fewer DNA polymerases Mn2+). Concentrations of Mg2+ or Mn2+ must typically be optimized. In some cases, additives can enhance PCR efficiency, specificity, and yield. The appropriate cycling parameters contribute to a successful PCR.
An overview of PCR applications and enzymes / kits provided by Roche Applied Science, is found at the Roche Special Interest Site Amplification–Innovative tools for PCR under "Find the optimal product for your application". In addition, refer to the interactive PCR Product Selection Guide linked to this site.

Materials

     
Related Links