Life Science

CryoStor Cryopreservation Protocol

Cryopreservation efficacy - which includes post-thaw recovery, viability, and functionality is of importance to both research and clinical applications. The cumulative stresses that result from the cryopreservation process and suboptimal freeze media result in cell death from necrosis and apoptosis.1-5 Cells and tissues can be prepared and preserved in ultra low temperature environments (-80°C to -196°C) using CryoStor cryopreservation solutions. Since these solutions are formulated to address the molecular biological aspects of cells during the cryopreservation process, they reduce the level of cryopreservation-induced delayed-onset cell death, thereby improving post-thaw cell viability and function. In addition, they need to include serum, proteins, or high levels of cytotoxic agents is eliminated with this protocol. In this video article, we will demonstrate the procedures for freezing, storage, and thawing of cells, as well as a viability assessment of the cells post-thaw.

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1.  Mathew, A.J. I m Losing Cell Viability and Function at Different Points in My Process, and I Don t Know Why! BioProcess International 8(6), 54-7 (2010).

2.  Baust, J.M., Snyder, K.K., Mathew, A.J., Van Buskirk, R.G., Baust, J.G. Steps to Improving Cryopreservation Outcome: Understanding Key Factors Influencing Efficacy. Bioscience Technology (2006).

3.  Van Buskirk, R.G., Snyder, K.K., Mathew, A.J., Baust, J.G., Baust, J.M. Navigating the post-preservation viability fog. Genetic Engineering News 38-39 (2006).

4.  VanBuskirk, R.G., Snyder, K.K., Baust, J.G., Mathew, A.J., Baust, J.M. Cryopreservation: it s not just about cell yield. BioProcess International 2-8 (2005).

5.  Snyder, K.K., VanBuskirk, R.G., Baust, J.M., Mathew, A.J., Baust, J.G. Biological packaging for the global cell and tissue therapy markets. BioProcessing Journal 1-7 (2004).