Life Science

MISSION® LentiPlex® Pooled shRNA Library Screening in Mammalian Cells

The MISSION LentiPlex Human shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The TRC 1.0 library consists of over 80,000 shRNA constructs from the TRC collection targeting 16,000+ human genes. The TRC Complete Library (1.0, 1.5, and 2.0) consists of 125,000+ clones and 20,000+ human genes (recommended)1. Each library is tested for shRNA representation before product release to ensure robust library coverage. The 1.0 and complete libraries are provided in a ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay. The 1.0 library is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. The complete library is pre-divided into 16 subpools spanning 6,000-8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.

Previous studies established a synergistic antitumor activity of TRAIL when combined with Paclitaxel in A549 cells, a human lung carcinoma cell line2,3. In this study we demonstrate the application of a pooled LentiPlex shRNA library to rapidly conduct a positive selection screen for genes involved in the cytotoxicity of A549 cells when exposed to TRAIL and Paclitaxel. One barrier often encountered with high-throughput screens is the cost and difficulty in deconvolution; we also detail a cost-effective polyclonal approach utilizing traditional sequencing.

To learn more, read the Biofiles article on Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways.

View the product information at MISSION LentiPlex Pooled shRNA Libraries.