Functional Genomics & RNAi Zinc Finger Nuclease (ZFN)

CompoZr^® Zinc Finger Nuclease Technology

Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations.

Double-strand breaks are important for site-specific mutagenesis in that they stimulate the cell’s natural DNA-repair processes, namely homologous recombination and Non-Homologous End Joining (NHEJ).

Using well-established and robust protocols, these cellular processes can be harnessed to generate precisely targeted genomic edits resulting in cell lines, including somatic cell lines, with targeted gene deletions (Knockouts), integrations, or modifications.

Method of the Year 2011 – Nature Methods

“Genome Editing with Engineered Nucleases”

For its annual choice to highlight an important research method for biological researchers Nature Methods has selected “Genome Editing with Engineered Nucleases” - Nat Methods. 2011 Dec 28; 9(1):26. In 2011 there were many high profile publications using zinc finger nucleases with a wide range of genomic edits in a variety of cell lines and species, see Zinc Finger Nuclease References.

2011 was also an important year as the leading genome editing technology, CompoZr ZFNs, had a reduction in price that the increased access of the most well-characterized and highly published genome editing technology in the market. Along with the reduction in price Knockout ZFNs are now available on the Sigma website for all human, mouse and rat genes providing easy access to the most common genome editing research application.

CompoZr ZFN Products

Knockout ZFNs

Custom ZFN Service

Targeted Integration Kits

Learning Center: What is ZFN Technology? How do ZFNs Work? ZFNs in Animals ZFN Webinar Series ZFN expert, Dana Carroll talks about the technology Frank Sleutel’s lab from the Netherlands Tool Box: Your Favorite Gene Search

Applications Center: ZFN References FAQs Life Science Application Center     Related Topics: RNAi Transgenics TargeTron  Cell Biology Stem Cell Biology Molecular Biology Core Bioreagents