LOCUS SM216 5636 bp DNA circular 2-OCT-2009 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|531048996| COMMENT VNTDBDATE|531049277| COMMENT LSOWNER| COMMENT VNTNAME|SM216| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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Location/Qualifiers misc_feature 659..1462 /vntifkey="21" /label=HA-L misc_feature 1463..1488 /vntifkey="21" /label=Splice\acceptor misc_feature 2582..3418 /vntifkey="21" /label=HA-R misc_feature 2529..2580 /vntifkey="21" /label=MCS misc_feature 135..441 /vntifkey="21" /label=f1\origin\(+) misc_feature 3833..4500 /vntifkey="21" /label=pUC\ori misc_feature 4651..5508 /vntifkey="21" /label=AmpR primer 1176..1195 /vntifkey="27" /label=Cel-I\F2 primer 2743..2762 /vntifkey="27" /label=Cel-IR2 misc_feature 1497..1568 /vntifkey="21" /label=2A misc_feature 1575..2174 /vntifkey="21" /label=Puro\resistance\gene misc_feature 2175..2292 /vntifkey="21" /label=unique\to\Puro\cassette polyA_signal 2328..2522 /vntifkey="25" /label=polyA misc_feature 1717..1737 /vntifkey="21" /label=Puro\-160\R\primer BASE COUNT 1147 a 1686 c 1461 g 1342 t ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacctg 661 ctttctctga ccagcattct ctcccctggg cctgtgccgc tttctgtctg cagcttgtgg 721 cctgggtcac ctctacggct ggcccagatc cttccctgcc gcctccttca ggttccgtct 781 tcctccactc cctcttcccc ttgctctctg ctgtgttgct gcccaaggat gctctttccg 841 gagcacttcc ttctcggcgc tgcaccacgt gatgtcctct gagcggatcc tccccgtgtc 901 tgggtcctct ccgggcatct ctcctccctc acccaacccc atgccgtctt cactcgctgg 961 gttccctttt ccttctcctt ctggggcctg tgccatctct cgtttcttag gatggccttc 1021 tccgacggat gtctcccttg cgtcccgcct ccccttcttg taggcctgca tcatcaccgt 1081 ttttctggac aaccccaaag taccccgtct ccctggcttt agccacctct ccatcctctt 1141 gctttctttg cctggacacc ccgttctcct gtggattcgg gtcacctctc actcctttca 1201 tttgggcagc tcccctaccc cccttacctc tctagtctgt gctagctctt ccagccccct 1261 gtcatggcat cttccagggg tccgagagct cagctagtct tcttcctcca acccgggccc 1321 ctatgtccac ttcaggacag catgtttgct gcctccaggg atcctgtgtc cccgagctgg 1381 gaccacctta tattcccagg gccggttaat gtggctctgg ttctgggtac ttttatctgt 1441 cccctccacc ccacagtggg gcctgacctc ttctcttcct cccacagggc ctcgagagat 1501 ctggcagcgg agagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg 1561 gccctaggct cgaaatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg 1621 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca 1681 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc 1741 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct 1801 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg 1861 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc 1921 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg 1981 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg 2041 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct 2101 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca 2161 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac 2221 cccatggctc cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag 2281 gcccaccgac tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag 2341 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 2401 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 2461 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 2521 caaccggtgc tagaattctc atgatatctt ccgtcgacgc tctctagagc tagcggccgc 2581 tactagggac aggattggtg acagaaaagc cccatcctta ggcctcctcc ttcctagtct 2641 cctgatattg ggtctaaccc ccacctcctg ttaggcagat tccttatctg gtgacacacc 2701 cccatttcct ggagccatct ctctccttgc cagaacctct aaggtttgct tacgatggag 2761 ccagagagga tcctgggagg gagagcttgg cagggggtgg gagggaaggg ggggatgcgt 2821 gacctgcccg gttctcagtg gccaccctgc gctaccctct cccagaacct gagctgctct 2881 gacgcggctg tctggtgcgt ttcactgatc ctggtgctgc agcttcctta cacttcccaa 2941 gaggagaagc agtttggaaa aacaaaatca gaataagttg gtcctgagtt ctaactttgg 3001 ctcttcacct ttctagtccc caatttatat tgttcctccg tgcgtcagtt ttacctgtga 3061 gataaggcca gtagccagcc ccgtcctggc agggctgtgg tgaggagggg ggtgtccgtg 3121 tggaaaactc cctttgtgag aatggtgcgt cctaggtgtt caccaggtcg tggccgcctc 3181 tactcccttt ctctttctcc atccttcttt ccttaaagag tccccagtgc tatctgggac 3241 atattcctcc gcccagagca gggtcccgct tccctaaggc cctgctctgg gcttctgggt 3301 ttgagtcctt ggcaagccca ggagaggcgc tcaggcttcc ctgtccccct tcctcgtcca 3361 ccatctcatg cccctggctc tcctgcccct tccctacagg ggttcctggc tctgctctcc 3421 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta 3481 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 3541 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 3601 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 3661 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 3721 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 3781 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 3841 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 3901 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 3961 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 4021 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 4081 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 4141 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 4201 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 4261 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 4321 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 4381 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 4441 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 4501 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 4561 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 4621 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 4681 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 4741 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 4801 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 4861 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 4921 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 4981 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 5041 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 5101 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 5161 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 5221 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 5281 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 5341 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 5401 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 5461 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 5521 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 5581 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac //