Online Enzyme Quiz Answers

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1. There are a large number of proteases offered among Sigma’s protein products. Which protease is extracted from the fungus Tritirachium album?

  • Proteinase K

2. Which enzyme is implicated in GSD Type I Von Gierke Disease?

  • Glucose-6-phosphatase

3. Sigma's NEW PRECISIO Kinases exhibit high purity and specific activity. HIPK1 or homeodomain-interacting protein kinase 1 is a ser/thr protein kinase. HIPK1 expression is elevated for example in breast cancer cell lines. What is the approximate molecular weight of HIPK1?

  • 71 kDa

4. Lectins are carbohydrate-binding proteins, macromolecules that are highly specific for sugar moieties. Which sugar moiety is Lectin from Triticum vulgaris specific for?

  • N-acetyl-β-D-glucosamine

5. Superoxidase Dismutase (SOD) is a cell-signalling enzyme important for the defence of cells against the toxic effects of oxygen radicals. It catalyzes the reduction of superoxide anions to hydrogen peroxide. What is the approximate KM for O2- for bovine SOD?

  • 0.35 mM

6. Horseradish peroxidase is widely used as a label for immunoglobulins in many different immunochemistry applications including ELISA, immuno-blotting and immunohistochemistry. How much of the world's horseradish is produced in the American Bottoms area of the Mississippi Valley?

  • 60%

7. The four main types of casein comprise approximately 80% of the total protein in bovine milk. Fragments of casein have been implicated in various biological functions. What biological function is exhibited with α-s2 Casein?

  • Antibacterial activity

8. Sigma Life Science offers two product lines for fast qPCR: KiCqStart qPCR ReadyMixes and the LuminoCt qPCR Reagents. How many different formulations are available for the KiCqStart qPCR ReadyMixes to suit all real-time PCR instruments?

  • 4

9. O-Linked glycans are most commonly attached to the peptide chain through serine (Ser) or threonine (Thr) residues. What are the most common O-linked glycans?

  • Mucin-type glycans

10. Lysostaphin is a zinc endopeptidase that has been found useful for cell lysis and also as a potential anti-microbial therapeutic. What is its pH Optimum for activity?

  • 7.5

11. There are several different types of enzyme classes. Oxidoreductases is one of them. What is an example of substrate that this class can act on?

  • Acting on NAD or NADP

12. The Protease Finder tool can help you identify proteases needed for precision protein cleavage. What endoprotease cleaves between two Proline residues?

  • Subtilisin

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