|
| http://www.sigma-aldrich.com/handh |
|
||
|
||
|
||
|
||
|
||
| Celestine Blue Method - Lendrum |
| Product No. | Name |
| 221260 | Ammonium iron(III) sulfate dodecahydrate |
| 206342 | Celestine blue |
| 258105 | Sulfuric acid |
| R3154 | Reagent Alcohol, 70% |
| 258148 | Hydrochloric acid |
| G7893 | Glycerol |
| R3404 | Reagent Alcohol, 95% |
| E4382 | Eosin Y disodium salt |
| 242853 | Acetic acid |
| 50% off our new preblended reagent alcohols used for histological processing and staining procedures. (US Customers Only) New to our histology consumables collection, our preblended reagent alcohols are available in convenient 70%, 80% and 95% concentrations, R3154, R3279 and R3404 respectively. Reagent alcohols are denatured ethanol with methanol and isopropyl alcohols. Used in combination with our existing 100% Reagent Alcohol (Product no. R8382), these non-controlled alcohols are designed to fulfill your specimen processing and staining needs. When ordering please reference promo code 467. Order now, offer expires December 31, 2006. No limit on purchases |
| REFERENCES: Lendrum, AC; Celestin Blue as a nuclear stain. J Pathol Bacteriol 40:415-16, 1935 Brown, GG; An Introduction to Histotechnology, Appleton-Century-Crofts, New York 1978 |
| Amount | Name |
| 5% Ammonium Iron(III) Sulfate Solution | |
| 15 g | Ammonium Iron(III) Sulfate, Dodecahydrate |
| 300 ml | Distilled or deionized water |
| Procedure: Dissolve the Ammonium Iron(III) Sulfate in the water. Mix on a stire late overnight to ensure complete dissolution. | |
| Celestine Blue Solution | |
| 1.5 g | Sodium Phosphate Monobasic |
| 6 ml | Sulfuric Acid |
| 42 ml | Glycerol, ACS |
| Procedure: Add the Celestine Blue to the 5% Ammonium Iron(III) Sulfate Solution and shake well in a flask. Boil for 3 minutes and allow to cool. Add the Sulfuric Acid and mix well. Add the Glycerol and mix well. Store in a brown bottle. Solution should be stable for several months. | |
| Acid Alcohol | |
| 300 ml | Reagent Alcohol, 70% |
| 6 ml | Hydrochloric Acid |
| Procedure: Add the Hydrochloric Acid to the Reagent Aclohol and mix well. Store at room temperature. | |
| Alcoholic Eosin Y Solution | |
| 300 ml | Reagent Alcohol, 95% |
| 1.5 g | Eosin Y, disodium salt, certified by the BSC |
| 1.5 ml | Acetic Acid, ACS Reagent |
| Procedure: Add the Eosin Y to the 95% Reagent Alcohol and mix 1-2 hours. Slowly add the Acetic Acid and mix overnight. Seal the mixing container with Parafilm while mixing overnight. Allow to age at least 2-3 days before use. Store at room temperature. Solution should be stable for several months. |