固相抽出(SPE)

Supel™-Select HLB SPE

 

親水性・親油性 逆相系ポリマー

SupelcoのSupel-Select HLB SPEは親水基を導入したスチレン系ポリマーで、幅広い極性の化合物を保持するようにデザインされました。 この保持メカニズムは主として疎水性相互作用に基づきますが、親水基を導入しているため高極性化合物も保持します。

hlb-spe01
担体: 親水性基導入スチレン/ジビニルベンゼン共重合体
使用pH範囲: 0-14
粒子径: 55-60 μm
MS使用: Yes
表面積: 400-410 m2/g
ポア容量: 0.88 mL/g
ポアサイズ: 87 Å

サンプルのご依頼はこちら

他社相当品との保持比較結果

Group 1EPA Method 1694 Pharmaceutical Compounds

回収率(%) Supel-Select HLB 他社相当品
メタノール/ アセトニトリル
50:50 溶出液
メタノール
溶出液
メタノール/ アセトニトリル
50:50 溶出液
メタノール
溶出液
1,7-Dimethylxanthine 14.41 56.15 83.59 68.65
Acetaminophen 119.5 112.3 136.48 141.18
Ampicillin 0 14.1 0 12.5
Azithromycin 101 52.99 58.9 50.34
Caffeine 70.71 86.23 81.43 85.51
Carbadox 80.77 73.1 59.76 69.46
Carbamazepine 81.47 79.63 83.38 92.86
Cefotaxime 45.49 74.14 20.44 61.79
Ciprofloxacin 21.73 57.14 50.58 94.74
Clinafloxacin 69.46 59.29 74.29 70.18
Cloxacillin 42.83 0 28.48 15
Codeine 3.75 8.72 66.02 64.18
Cotinine 9.48 8.12 65.5 34.2
Dehydronifedipine 63.84 77.11 87.74 74.4
Digoxigenin 69.96 80.12 76.86 83.28
Digoxin 80.05 NA 50.33 NA
Diltiazem 114.46 124.52 123.47 138.04
Diphenhydramine 100 119.02 99.38 82.48
Enrofloxacin 55.53 58.37 40.91 80.42
Erythromycin hydrate 95.65 63.87 59.68 57.1
Flumeqine 83.1 56.27 44.15 69.57
Fluoxetine 61.5 55.35 77.46 55.76
Lincomycin 2.62 7.98 44.74 31.98
Lomefloxacin 23.37 67.09 54.74 97.58
Miconazole 84.02 52.03 71.13 81.71
Norfloxacin 18.15 41.29 60.49 89.35
Norgestimate 392.24 39.36 215.3 41.35
oxacin 23.1 59.14 56.58 75.96
Ormetoprim 27.54 86.8 66.27 103.55
Oxacillin 0 19.12 0 6.24
Oxolinic acid 64.97 44.26 50.27 43.82
Penicillin G 0 6.08 0 6.06
Penicillin V 27.89 55.12 81.87 38.77
Roxithromycin 32.64 39.3 19.73 31.33
Sarafloxacin 53.21 56.24 58.18 67.63
Sulfachloropyridazine 78.05 67.09 49.19 62.39
Sulfadiazine 104.79 70.16 77.84 76.7
Sulfadimethoxine 73.65 51.34 45.27 63.09
Sulfamerazine 93.64 76.88 57.73 89.25
Sulfamethazine 94.59 74 78.11 73.4
Sulfamethizole 85.61 74.48 48.99 76.72
Sulfamethoxazole 72.46 56.14 49.35 71.43
Sulfanilamide 10.11 7.49 24.8 32.84
Sulfathiazole 104.27 73.91 66.82 68.12
Thiabenzadole 95.54 77.95 86.99 83.65
Trimethoprim 25.68 65.2 83.91 100.84
Tylosin 97.7 44.26 46.36 26.11
Virginiamycin 71.92 56.16 68.08 63.36

 

※各社保持力の強弱が見受けられるので溶出溶媒の微調整が必要です
詳細は“Reporter Volume 27.3  P8-10

低いバックグランド

Supel-Select HLB SPEのブランクはLC-UVとLC-MSの両方を用い測定しています。 高感度分析を必要とする場合、固相抽出カラムや使用器具のブランクが問題となります。 図2.はSupel-Select HLB SPEと他社固相抽出カラムをアセトニトリルで溶出させ、LC-UVで測定したものです。 その結果Supel-Select HLB SPEは他社製品に比べ1/4以下の低ブランク量でした。Supelco では厳格な品質管理によりロット差の無い安定した製品を提供します。

製品検査項目

  • 回収率
  • 充填剤ブランク:LC-UV およびLC-MS
  • 粒子径
  • 嵩比重
  • ポアサイズ
  • ポア容量
hlb-spe02
シリンジ型固相抽出管
充填剤量/シリンジサイズ
入数 CAT.NO.
30 mg/1 mL   100 54181-U
60 mg/3 mL   50 54182-U
200 mg/6 mL   30 54183-U
500 mg/12 mL   20 54184-U
1 g/20 mL    20 54186-U
96-ウェルプレート タイプ
充填剤量/well
入数 CAT.NO.
30 mg /well 1 575661-U
60 mg/ well 1 575662-U

関連資料

Supel-Select HLB SPE 取扱説明書 (59KB PDF)
Supel-Select HLB SPE パンフレット (583KB PDF)

参考文献

  • Sameera S. et al. "Characterization of Date(Deglet Nour) Seed Free and Bound Polyphenols by High-Performance Liquid Chromatography-Mass Spectrometry"Journal of Food Science,(2017) vol. 82
  • Anna H. et al."LC–MS measurment of free steroids in mussels (Mytilus trossulus) from the southern Baltic Sea"Journal of Pharmaceutical and Biomedical Analysis 117 (2016) 311–315
  • Anna H. et al."Simvastatin Effect on Calcium and Silicon Plasma Levels in Postmenopausal Women with Osteoarthritis"Biol Trace Elem Res (2016) 171:1–5
  • Wei C. et al. "Simultaneous determination of 20 trace organic chemicals in waters by solid-phase extraction (SPE) with triple-quadrupole mass spectrometer (QqQ-MS) and hybrid quadrupole Orbitrap high resolution MS (Q-Orbitrap-HRMS)"Chemosphere 163 (2016) 99-107
  • Ana Y. et al. "Development and Validation of Chronopotentiometric Method for Imidacloprid Determination in Pesticide Formulations and River Water Samples"Hindawi Publishing Corporation International Journal of Analytical Chemistry Volume 2016
  • Renata B. et al. "Selective determination of cocaine and its metabolite benzoylecgonine in environmenta samples by newly developed sorbent materials"Talanta 146(2016)401–409
  • Lucia B. et al. "Validated LC–MS-MS Method for Multiresidual Analysis of 13 Illicit Phenethylamines in Amniotic Fluid"Journal of Analytical Toxicology, Volume 40, Issue 3, 1  (2016), Pages 194–200
  • M. Waso et al. "Presence of microbial and chemical source tracking markers in roof-harvested rainwater and catchment systems for the detection of fecal contamination"Environmental Science and Pollution Research (2016)
  • Sopheak N. et al. "Distribution of phthalates, pesticides and drug residues in the dissolved, particulate and sedimentary phases from transboundary rivers"Science of the Total Environment 521–522 (2015) 152–159
  • 相澤ら"窒素をキャリヤーガスとする低圧GC/MSによる水道水中のハロ酢酸類,ホルムアルデヒド及びフェノール類の定量"BUNSEKI KAGAKU Vol.64 (2015) 705-713
  • Katerina J. et al. "Residues of selected antibiotics in the South Moravian Rivers, Czech Republic"Neuroendocrinology Letters,(2015) Volume 36 Suppl. 1
  • Sandra Z. et al. "Sex-related differences in steroid concentrations in the blue mussel (Mytilus edulis trossulus) from the southern Baltic Sea"Comparative Biochemistry and Physiology, Part A 183 (2015) 14–19
  • Stéphane B. et al."Analysis of selected antibiotics in surface freshwater and seawaterusing direct injection in liquid chromatography electrosprayionization tandem mass spectrometry"Journal of Chromatography A, 1338 (2014) 38–43
  • R. El-O. et al. "An experimental design approach to the optimisation of pesticide extraction from water"Anal. Methods, (2014), 6, 6514
  • El-Osmani R. et al. "Solid Phase Extraction of Organochlorine Pesticides Residues in Groundwater (Akkar Plain, North Lebanon)"Int. J. Environ. Res., 8(4):903-912, Autumn 2014
  • Bradley J. N. et al. "Green tea diet decreases PCB 126-induced oxidative stress in mice by upregulating antioxidant enzymes"J Nutr Biochem. (2014) February ; 25(2): 126–135.
  • Chiara Da P. et al. "Development of a liquid chromatography/tandem mass spectrometry method to investigate the presence of biomarkers of DNA damage in urine related to red meat consumption and risk of colorectal cancer"Rapid Commun. Mass Spectrom. (2013), 27, 2493–2503