UHPLC

Ascentis Express 2.0um UHPLCカラム

Index
・アプリケーション
・論文リスト
・製品リスト
 

On-demand webinar:
Efficient Separation in UHPLC
Using 2.0 µm Fused-Core Columns

Ordering Information
C18, F5, OH5, HILIC, C8,
Phenyl-Hexyl, RP-Amide, ES-Cyano



Ascentis® Express 2.0 μm
UHPLC Columns
(1.3MB PDF)

 

各カラムにおけるピーク形状と圧力比較

Conditions
    column:  Ascentis Express C18 ,10 cm x 2.1 mm I.D., 2.0 µm (50813-U)
    mobile phase: (A) water with 0.1% formic acid;
                       (B) acetonitrile with 0.1% formic acid; (65:35, A:B)
    flow rate:  0.8 mL/min
      temp.:  35 °C
      det:  UV, 240 nm
    injection:  0.5 µL
      sample:  50 µg/mL in 80:20, water:methanol
    instrument:  Dionex Ultimate 3000

 

Plates per Pressure Comparison Against Core-Type or Solid-Core sub-2 µm UHPLC Columns

Conditions
    column:      5 cm x 2.1 mm I.D.
    mobile phase:      A:acetonitrile; B:water; (50:50 – 47:53, A:B)
    flow rate:      0.5 mL/min
    temp.:      35 °C
    instrument:      Shimadzu Nexera

 

Columns based on Ascentis Express 2 µm particles produce 30% higher efficiency per unit pressure than UHPLC columns based on smaller sub-2 µm solid core particles.

 

Ascentis Express 2.0 µm Fused-Core Silica Characteristic

 

Pore Size 90 Å
Max Temp. 60 °C
Pressure 1000 bar (14500 psi)
Features Endcapped
Operating pH Range 2-9
Surface Area 120 m2/g

Ascentis Express アプリケーション例

 

論文リスト

・製薬・臨床
・食品
・環境

Ascentis Express 製品リスト

Ascentis Express Hyper-Fast Separations
High-Definition Resolution
Super-Sensitive
Super-Rugged
Technical Resource GuideTechnical Resources Fast HPLC Application SupportApplications Support
 
Product Selection Guide

Find the right Ascentis Express product to best suit your needs.

C18 HPLC Columns

2.0 µm | 2.7 µm | 5 µm
The first choice for starting a new method.

2.7 µm
Separate compounds that are challenging to resolve or elute early on C18 or other phenyl phases

RP-Amide HPLC Columns

2.0 µm | 2.7 µm | 5 µm
Combining an embedded polar group stationary phase and Fused-Core particles.

Phenyi-Hexyl HPLC Columns

2.0 µm | 2.7 µm | 5 µm
Combining the popular phenyi-hexyl stationary phase and Fused-Core particles.

C8 HPLC Columns

2.0 µm | 2.7 µm | 5 µm
Ideal for non-polar compounds that are reatined too strongly by C18.

ES-Cyano HPLC Columns

2.0 µm | 2.7 µm | 5 µm
Provides stable, reversed-phase packing for basic, acidic or neutral compounds.

HILIC Si HPLC Columns

2.0 µm | 2.7 µm | 5 µm
Ideal for many classes of polar compounds.

F5 HPLC Columns

2.0 µm | 2.7 µm | 5 µm
Separate closely related compounds.

OH5 HPLC Columns

2.0 µm | 2.7 µm | 5 µm
Pentahydroxy phase for fast analysis of polar compounds.

Peptide ES-C18 HPLC Columns

2.7 µm
Separate high molecular weight compounds such as peptides and small proteins.

 

Ascentis Express 固定相選択ガイド