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抗体

細胞小器官マーカー用抗体

新しいタンパク質が発見された際、その機能解析だけでなく細胞内局在も重要な情報の1つです。さらに、細胞内局在を知ることは、そのタンパク質の機能を知る上で大変な助けとなります。タンパク質の局在を調べる最も一般的な手法の1つは、タンパク質特異的な抗体と細胞小器官特異的な抗体を用いて2重染色を行うことです。

シグマアルドリッチでは、細胞小器官マーカーとして有用な細胞小器官特異的もしくは細胞小器官に局在するタンパク質特異的な多くの抗体ラインナップをご提供いたします。これらの細胞小器官マーカーを用いることで共局在の有無を調べることも可能ですし、細胞小器官を単離する際には濃縮率を測定することも可能です。

シグマアルドリッチの細胞小器官マーカー用抗体を用いることで、高解像度の蛍光標識画像を取得することが出来ます。抗体作製のために使用した抗原ペプチドは多くの生物種を認識し、且つ非特異な交差反応性を抑えたデザインとなっています。抗体の特異性はウエスタンブロッティングおよび抗原による阻害試験により検証されています。ですので、これらの抗体はウェスタンブロッティングや細胞染色以外にも組織染色や免疫沈降などの幅広いアプリケーションにも使用可能です。

 

ER and ERGIC

Endoplasmic Reticulum (ER): Anti-Protein Disulfide Isomerase (PDI) (Cat. No. P7496). Endoplasmic Reticulum-Golgi intermediate compartment (ERGIC): Anti-ERGIC-53/p58, Cy3 Conjugate (Cat. No. E6782).
Organelle Suborganelle Name Host Form Application
(略号一覧)
Species Reactivity Cat. No.
Endoplasmic
Reticulum (ER) 
ER Lumen Anti-GRP78/BiP (ET-21) rabbit IgG fraction ICC, WB Xenopus, chicken, hamster,
human, mouse, rat
G9043
Anti-GRP78/BiP (GL-19) rabbit IgG fraction ARR, ICC, WB hamster, human, mouse G8918
Anti-GRP94 (C-terminal) rabbit affinity isolated antibody ARR, ICC, IF (i), IP, WB canine, human, mouse G4420
Anti-Protein Disulfide Isomerase rabbit IgG fraction IF (i), IP, WB bovine, human, mouse, rat P7496
Anti-Protein Disulfide Isomerase
(MD-12)
rabbit affinity isolated antibody IF (i), IP, WB CL bovine, human, mouse, rat P7372
Anti-Protein Disulfide Isomerase
(DL-11)
rabbit Affinity isolated antibody IF (i), IP, WB CL human, mouse, rat P7122
Anti-Calreticulin rabbit IgG fraction ARR, IF (i), IP, WB canine, human C4606
Monoclonal Anti-Calreticulin mouse tissue culture supernatant ELISA (i), ICC, IHC,
WB, FC
human C7492
Monoclonal Anti-ERp57 mouse purified immunoglobulin ARR, ELISA (i), ICC, IHC,
WB, FC
human, mouse E5031
ER Membrane Anti-Calnexin rabbit IgG fraction ARR, IF (i), IP, WB canine, human, mouse, rat C4731
Monoclonal Anti-Calnexin mouse purified immunoglobulin ELISA (i), ICC, IHC,
WB, FC
human C7617
ERGIC Anti-ERGIC-53/p58 rabbit affinity isolated antibody IF (i), WB CL human, mouse, rat E1031
Anti-ERGIC-53/p58−Cy™3 rabbit affinity isolated antibody IF (d) human, mouse E6782

 

Golgi, COPI, COPII, GW Bodies, and Stress Granules

Golgi Complex: Anti-TGN38 (Cat. No. T9826). GW Bodies: Anti-DCP2 (Cat. No. D6319). COPII Vesicles: Anti-Sec23 (Cat. No. S7696). Stress Granules: Anti-FMR1 (C-terminal) (Cat. No. F4055).
Organelle Suborganelle Name Host Form Application
(略号一覧)
Species Reactivity Cat. No.
Golgi Apparatus cis-Golgi and
cis-Golgi
network (CGN)
Anti-GM130 (C-terminal) rabbit affinity isolated antibody IF (i), IP, WB CL human, mouse, rat G7295
Monoclonal Anti-Golgi 58K
Protein/Formiminotransferase
Cyclodeaminase (FTCD)
mouse ascites fluid EM, IF (i), WB bovine, canine, hamster,
human, kangaroo rat,
monkey, mouse, pig, rat
G2404
trans-Golgi and
trans-Golgi
network (TGN)
Monoclonal Anti-Syntaxin 6 mouse purified immunoglobulin ARR, ELISA (i), ICC, IP, WB human, mouse, rat S9067
Anti-TGN38 rabbit affinity isolated antibody IF (i) rat T9826
Anti-TGN46 rabbit affinity isolated antibody IF (i), IP, WB CL human T7576
Monoclonal Anti-TGN46 mouse purified immunoglobulin IP, WB human SAB4200235
CopI Vesicles Monoclonal Anti-β-COP mouse ascites fluid ICC, IF (i), IP bovine, canine, chicken,
goose, hamster, human,
kangaroo rat, monkey,
rabbit, rat
G2279
Monoclonal Anti-β-COP mouse ascites fluid ARR, IF (i), WB hamster, human, monkey,
rat
G6160
CopII Vesicles Anti-Sec23 rabbit affinity isolated antibody IF (i), WB CL human, mouse, rat S7696
P-Bodies/GW-Bodies Anti-DCP1A (C-terminal) rabbit affinity isolated antibody IF (i), IP, WB human, mouse, rat
(predicted)
D5444
Anti-DCP1A (N-terminal) rabbit affinity isolated antibody IF (i), IP, WB human, mouse, rat D5694
Anti-DCP2 (C-terminal) rabbit affinity isolated antibody IF (i), WB human, mouse, rat D6319
Anti-DCP2 (N-terminal) rabbit affinity isolated antibody IF (i), WB human, mouse (predicted),
rat (predicted)
D6194
Anti-GW182 rabbit affinity isolated antibody IF (i) human G5922
Stress Granules Anti-FMR1 (C-terminal) rabbit affinity isolated antibody IF (i), IP, WB human, mouse, rat F4055

 

Endosomes, Lysosomes, and Peroxisomes

Early Endosomes: Anti-Rab5 (Cat. No. R4654). Late Endosomes: Anti-Syntaxin 7 (Cat. No. S4819). Lysosome: Anti-LAMP1, Cy3
Conjugate (Cat. No. L0419).
Peroxisome: Anti-PMP70 (Cat. No. P0497).
Organelle Suborganelle Name Host Form Application
(略号一覧)
Species Reactivity Cat. No.
Endosomes Early endosomes Anti-Early Endosomal Antigen 1
(C-terminal)
rabbit affinity isolated antibody IF (i), WB CL human, mouse, rat E3906
Anti-Early Endosomal Antigen 1
(N-terminal)
rabbit affinity isolated antibody IF (i), WB CL human, mouse, rat E4156
Monoclonal Anti-EEA1 mouse purified immunoglobulin IF (i), IP, WB human, mouse, rat E7659
Monoclonal Anti-EEA1 mouse purified immunoglobulin WB human, mouse, rat E8034
Anti-Rab5 rabbit affinity isolated antibody IF (i), WB human, mouse, rat R4654
Monoclonal Anti-Rab5 mouse purified immunoglobulin ARR, ELISA (i), ICC, WB bovine, cat, human,
mouse, rat
R7904
Anti-Rab7 rabbit affinity isolated antibody IF (i), WB human, mouse, rat R4779
Monoclonal Anti-Rab7 mouse purified immunoglobulin ARR, ELISA (i), ICC, IP, WB bovine, chicken, human,
monkey, mouse, rat
R8779
Anti-Rab4 rabbit affinity isolated antibody IF (i), WB human, mouse, rat R5780
Anti-Syntaxin 8 rabbit affinity isolated antibody ARR, IP, WB rat S8945
Late endosomes Anti-Rab9 rabbit affinity isolated antibody IF (i), WB human, mouse, rat R5279
Monoclonal Anti-Rab9 mouse purified immunoglobulin ARR, ICC, IP, WB canine, hamster, human,
mouse, rat
R5404
Anti-Syntaxin 7 rabbit IgG fraction IF (i), WB rat S4819
Lysosomes Monoclonal Anti-Cathepsin D mouse ascites fluid ARR, ELISA (i), IHC (p),
WB
human C0715
Anti-LAMP1 rabbit affinity isolated antibody IF (i) human, mouse, rat L1418
Anti-LAMP2 rabbit affinity isolated antibody IF (i), WB CL human (predicted),
mouse, rat
L0668
Anti-LAMP1−Cy™3 rabbit affinity isolated antibody IF (d) human, mouse, rat L0419
Peroxisomes Anti-PMP70 rabbit affinity isolated antibody IF (i), IP, WB CL human, mouse, rat P0497
Monoclonal Anti-PMP70 mouse purified immunoglobulin WB human, mouse, rat SAB4200181
Monoclonal Anti-Catalase mouse purified immunoglobulin ARR, ELISA (i), ICC,
IHC, WB
bovine, human, mouse, rat C0979

 

Nucleus and Nuclear Bodies

Nucleolus: Anti-Nucleolin (Cat. No. N2662). SAM68 Body: Anti-SAM68 (C-Terminal) (Cat. No. S9575). Paraspeckles: Anti-PSPC1 (N-Terminal) (Cat. No. SAB4200068).
Organelle Suborganelle Name Host Form Application
(略号一覧)
Species Reactivity Cat. No.
Nucleus (Selected) Monoclonal Anti-LAP2 (TMPO) mouse purified immunoglobulin ARR, ICC, WB human L3414
Nuclear Bodies Nucleolus Anti-Nucleolin rabbit affinity isolated antibody IF (i), IP, WB human, mouse, rat
N2662
Anti-Nucleolin−Atto 488 rabbit affinity isolated antibody IF (d) human, mouse, rat N6288
Cajal bodies Monoclonal Anti-Coilin mouse purified immunoglobulin ARR, ICC, WB human C1862
SAM68 bodies Anti-Sam68 (C-terminal) rabbit affinity isolated antibody IF (i), IP, WB human S9575
Paraspeckels Anti-PSPC1 (N-terminal) rabbit affinity isolated antibody
IF (i), WB CL
human SAB4200068
Anti-NONO (N-terminal) rabbit affinity isolated antibody IF (i), IP, WB human N8789
Anti-NONO (C-terminal) rabbit affinity isolated antibody IF (i), IP, WB human N8664
PML bodies Monoclonal Anti-PML mouse purified immunoglobulin ARR, ELISA (i), ICC, IP, WB hamster, human, mouse P6746
Nuclear speckles Monoclonal Anti-Splicing Factor
SC-35
mouse ascites fluid ELISA (i), ICC, IF (i), IP Drosophila, frog, human,
newt, rat
S4045
Nuclear membrane
lamina
Anti-Lamin A (C-terminal) rabbit affinity isolated antibody IF (i), WB human, mouse, rat
L1293
Anti-Lamin A−Atto 647N rabbit affinity isolated antibody IF (d) human L3544
Centromere Anti-CENP-E rabbit IgG fraction ARR, ICC, WB human C7488
Anti-INCENP rabbit IgG fraction ARR, IF (i), WB CL human, mouse, rat I5283

 

Mitochondria, Centrosome, and Plasma Membrane

Mitochondria Outer Membrane:
Anti-Cytochrome c (Cat. No. C9616).
Caveolae: Monoclonal Anti-Caveolin
1 Clone: CAV1 (Cat. No. SAB4200216).
Lipid Rafts: Anti-Flotillin 1 (Cat. No. F1180).
Organelle Suborganelle Name Host Form Application
(略号一覧)
Species Reactivity Cat. No.
Mitochondria Inner-membrane Anti-UCP-1 rabbit affinity isolated antibody IHC (p), WB human, mouse, rat U6382
Anti-UCP-3 rabbit affinity isolated antibody IHC (p), WB human, mouse, rat U7757
Outer-membrane Anti-VDAC/Porin rabbit IgG fraction ARR, WB human, mouse, rat V2139
Monoclonal Anti-TOM22 mouse purified immunoglobulin ARR, ICC, WB human, monkey T6319
Monoclonal Anti-Tom22−Atto 488 mouse purified immunoglobulin IF (d) human, monkey T4327
Anti-Mitofusin-2 (N-Terminal) rabbit affinity isolated antibody IF (i), IP, WB CL human, mouse, rat M6319
Anti-Mitofusin 2 rabbit affinity isolated antibody IP, WB CL human, mouse, rat M6444
Anti-Cytochrome c sheep affinity isolated antibody IF (i), IHC (p), WB canine, human, mouse, rat C9616
Matrix Anti-Superoxide Dismutase
(MnSOD) (KC-19)
rabbit IgG fraction IF (i), WB human, rat S5569


Anti-Superoxide Dismutase
(MnSOD) (DD-17)
rabbit

affinity isolated antibody IF (i), IHC (p), WB human, rat S5069
Centrosome Anti-γ-Tubulin (AK-15) rabbit IgG fraction
ARR, ICC, WB
frog, human, rat
T3320
Anti-γ-Tubulin rabbit affinity isolated antibody IF (i), WB chicken, human, rat T5192
Monoclonal Anti-γ-Tubulin mouse ascites fluid

ELISA (i), ICC, WB bovine, canine, chicken,
hamster, human, mouse, rat

T6557
Monoclonal Anti-δ Tubulin mouse purified immunoglobulin ELISA (i), ICC, WB human T3950
Plasma
Membrane
  Anti-SNAP-23 (DS-19) rabbit IgG fraction IF (i), WB mouse S2319
Anti-SNAP-23 (TS-19) rabbit IgG fraction ARR, IF (i), WB mouse, rat S2194
Monoclonal Anti-Pan Cadherin mouse ascites fluid ARR, IF (i), IHC (p), WB hamster, mouse, rat,
bovine, chicken, human,
canine, sheep, frog, pig,
guinea pig, snake, goat,
rabbit
C1821
Clathrin coated pits Anti-Clathrin goat whole antiserum IF (i) human C8034
Monoclonal Anti-Clathrin
Heavy Chain
mouse purified immunoglobulin ARR, ICC, IP, WB bovine, human, mouse, rat C1860
Monoclonal Anti-Clathrin
Light Chain
mouse purified immunoglobulin ARR, ICC, IP, WB bovine, human, mouse,
rat, yeast
C1985
Caveolae Anti-Caveolin-1 rabbit IgG fraction ARR, IF (i), WB mouse, human, rat C3237
Anti-Caveolin-1 rabbit affinity isolated antibody IF (i), WB mouse, human, rat C4490
Anti-Caveolin-2 rabbit
affinity isolated antibody
IF (i), WB canine, human (predicted),
mouse, pig (predicted), rat
C9992
Monoclonal Anti-Caveolin-1 mouse tissue culture supernatant IF (i), IHC, WB, human, mouse, rat SAB4200216
Lipid Rafts Anti-Flotillin 1 rabbit affinity isolated antibody IF (i), WB human, mouse, rat F1180
Anti-Flotillin 1-Cy3 rabbit affinity isolated antibody IF (d) human, mouse, rat F8931
Anti-Flotillin 2 rabbit affinity isolated antibody IF (i), WB

human, mouse, rat F1805
Anti-Flotillin 2 (C-terminal) rabbit affinity isolated antibody IF (i), WB
human F1680

 

Application Abbreviation Table

 

Application Abbreviation
Array (antibody microarray) ARR
Capture ELISA ELISA (c)
Direct ELISA ELISA (d)
Flow Cytometry FC
Immunoblotting WB
Immunoblotting (chemiluminescent) WB CL
Immunocytochemistry ICC
Application Abbreviation
Immunoelectrophoresis IEP
Immunohistochemistry (formalin-fixed, paraffinembedded
sections)
IHC (p)
Immunohistochemistry (frozen sections) IHC (f )
Immunoprecipitation IP
Indirect ELISA ELISA (i)
Indirect immunofluorescence IF (i)