3D Hydrogels

Sigma® offers a broad portfolio of three-dimensional cell culture systems including MaxGel human Extracellular Matrix (ECM), HydroMatrix synthetic peptide, and mouse ECM, to support stem cell and other cell cultures. These products provide three-dimensional environments in which cells are better able to mimic their in vivo counterparts.

Culture cells in a way that resembles their in vivo environments
Promote cell growth and migration to support the proliferation of stem cell and other mammalian cells
Reduced level of growth factors promotes lot-to-lot consistency

HydroMatrix Peptide Hydrogel

A synthetic peptide nanofiber scaffold, HydroMatrix offers the precision and control of a synthesized matrix with the natural three-dimensional architecture of highly crosslinked peptide hydrogel. The HydroMatrix scaffold self-assembles from fluid precursors into a highly cross-linked peptide 3-dimensional hydrogel in response to changes in temperature or ionic strength.
By adjusting the concentration of the HydroMatrix solution, researchers are able to control the flexibility of the 3-D architecture, and tailor the structure to meet their individual needs. HydroMatrix promotes cell growth and migration and has been shown to support the proliferation of many cell types, including neural stem cells, neurons, glia, astrocytes, fibroblasts, and keratinocytes.

MaxGel Human ECM

Produced in vitro, MaxGel human ECM provides a rich three dimensional environment to promote cellular proliferation. MaxGel ECM contains extracellular matrix components including collagens, laminin, fibronectin, tenascin, elastin, a number of proteoglycans and glycosaminoglycans. The cell-cultured derived ECM effectively reproduces the cooperative interaction of epithelia and mesenchyme during development and in organotypic cell culture of skin. The human MaxGel ECM promotes cell growth and migration and has been shown to support the proliferation of many cell types, including neural stem cells, neurons, glia, astrocytes, fibroblasts, hepatocytes and keratinocytes.


Hystem® Stem Cell Culture

The HyStem platform offers you, the researcher, control over growth factor incorporation, attachment factor incorporation, ECM protein incorporation, rigidity of the hydrogel, and cell encapsulation vs. top plating Because HyStem is a synthesized matrix and not a biological extract, researchers are able to closely control the composition of their cells’ environment. HyStem’s components include chemically synthesized HyStem (thiolated Hyaluronic acid), Extralink (thiol-reactive crosslinker), and degassed Water and biologically purified Gelin-S (denatured collagen). HyStem kits are optimal for culturing stem cells whose natural environments are rich in hyaluronic acid. The HyStem hydrogel scaffold closely mimics the rich, natural extracellular matrix environment, complete with hyaluronic acid and collagen fibrils, while offering the flexibility to customize with appropriate growth factors, attachment factors, and proteins.

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CIB001 Cell-in-a-Box® Cell Encapsulation Kit
E1270 ECM Gel from Engelbreth-Holm-Swarm murine sarcoma liquid, BioReagent, suitable for cell culture
E6909 ECM Gel from Engelbreth-Holm-Swarm murine sarcoma growth-factor reduced, without phenol red, liquid, BioReagent, suitable for cell culture
A6982 HydroMatrix Peptide Cell Culture Scaffold mixture, powder
H4040 HydroMatrix Peptide Cell Culture Scaffold 24 well plate
H3915 HydroMatrix Peptide Cell Culture Scaffold 6 well plate
HYS020 HyStem® Cell Culture Scaffold Kit For 7.5 mL of hydrogel scaffold solution
HYSC020 HyStem®-C Cell Culture Scaffold Kit For 7.5 mL of hydrogel scaffold solution
HYSHP020 HyStem®-HP Cell Culture Scaffold Kit For 7.5 mL of hydrogel scaffold solution
L2020 Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane 1-2 mg/mL in Tris buffered NaCl, 0.2 μm filtered, BioReagent, suitable for cell culture
L6274 Laminin from human placenta liquid, BioReagent, suitable for cell culture
E0282 MaxGel ECM mixture, liquid
M1073 MaxGel ECM 96 well plate