 | A7226 | Adenoviral Vectors for Gene Therapy | This book provides detailed, comprehensive coverage of the gene delivery vehicles based on the adenovirus, which are important tools for gene therapy. These new therapeutic agents have great potential in the treatment of disease. The topics covered in this book range from the basic biology of adenoviruses, through the construction and purification of adenoviral vectors, cutting-edge vectorology and the use of adenoviral vectors in preclinical animal models, to regulatory issues which must be considered prior to the initiation of human clinical gene therapy trials. The broad scope provides a complete understanding of the development and use of adenoviral vectors.
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| | A2594 | Adenovirus: Methods and Protocols | A cutting-edge collection of readily reproducible methods for conducting research with adenoviruses, the premier and most widely used model in cell and molecular biology. The methods range from how to grow and titer adenoviruses and how to construct specific alterations in the adenovirus genome, to how to measure apoptosis induced by cells of the immune system, cytokines, and intrinsic apoptosis effectors. In addition, there are methods to study transcription and splicing with in vitro systems and for the adenovirus-mediated transformation of cells to a malignant state.
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 | A9721 | Adhesion Molecule Factsbook, 2nd ed. | Second Edition is completely updated, and expanded to reflect new advances. It contains over 80 entries on all currently cloned and characterized cell adhesion molecules, including the families of integrins, cadherins, selectins, members of the immunoglobulin superfamily, and other relevant receptors. It provides information on amino acid sequence of the most commonly studied organisms; gene organization and chromosomal location; alternative nomenclature; biological function; tissue distribution; disease association; database accession numbers.
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 | A6347 | Amino Acid Analysis Protocols | This book is a collection of classic and cutting-edge techniques for answering specific biological questions about amino acids. Methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high performance anion exchange chromatography, and mass spectrometry are presented. Each method is described in detail to ensure success and emphasizes sample preparation, particularly collection and storage of bodily fluids.
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 | A6222 | Angiotensin Protocols | The renin-angiotensin system has been in the spotlight for more than 100 years because of its vital role in blood pressure regulation, ion and fluid homeostasis, and tissue growth and remodeling. This book describes in step-by-step detail all the major molecular, biochemical, and functional techniques used in probing the renin-angiotensin system. These range from the use of embryonic gene targeting to create of mutant strains, to the functional investigation of components of the renin-angiotensin system. This comprehensive text translates expert knowledge into readily reproducible methods that enable all biomedical investigators successfully to explore the role of angiotensin in hypertension, congestive heart failure, chronic renal failure and stroke.
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 | A9221 | Antibiotic Resistance: Methods and Protocols | This book describes a series of molecular and physical methods designed to study the growing problem of antibiotic resistance, as well as facilitate new antibiotic research programs for its effective redress. Techniques range widely from those that provide rapid diagnosis via DNA amplification and phage display, to those for plotting the transmission of resistant organisms and investigating their epidemiology. Methods are readily adaptable to a wide range of resistant bacterial organisms. Techniques are provided for basic biology of antimicrobial resistance, and for developing new diagnostic tools.
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 | Z702986 | Antibiotics: Actions, Origins, Resistance | This text offers a comprehensive account of the structural classes of antibiotics. While most of the attention is on natural products, synthetic chemicals with antibiotic activity are also discussed. The book contains a section that examines how antibiotics block specific proteins acting in these essential bacterial processes and how the molecular structure of the small-molecule drugs enables their antibiotic activity. Section III explores the development of bacterial resistance to antibiotics. The fourth section addresses the molecular logic of antibiotic biosynthesis, starting with regulatory networks that control gene transcription of secondary metabolites in streptomycetes.
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 | A6346 | Antigen Processing and Presentation Protocols | Drawing on expertise, investigators describe step-by-step methods designed to question how major histocompatibility complex-binding peptides are generated, to test how peptides are delivered to MHC molecules, to analyze MHC peptide-binding patterns, and to assay the T-cell response to the MHC/peptide complex. Emphasis is given those technical steps critical for experimental success that are often omitted from methods published in the primary literature.
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| | Z718165 | Arabidopsis | The cruciferous weed Arabidopsis thialiana has become a model system for the study of an unusually wide variety of aspects of plant biology. As a result, nearly all aspects of Arabidopsis biology are now under investigation, more scientists are involved, and plant science as a whole has been invigorated.
This book is the first comprehensive account of what is known about the organism. The information is presented in the context of plant biology in general, with the properties of Arabidopsis mutants and the insights derived from their analysis as a unifying theme. The book's scope includes genetics, growth and development, biochemistry, physiology, and responses to pathogens and environmental stress.
This unique volume, in the classic tradition of the Cold Spring Harbor Monograph Series, is a landmark in plant science, essential reading for investigators at graduate student level and beyond, and a work of reference that will serve the field for years to come.
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 | Z705772 | Arabidopsis Protocols 2nd ed. | This collection of reproducible Arabidopsis protocols has been updated to reflect recent advances in plant biology, the completion of the Arabidopsis genome sequence—essential for studying plant function, and the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics. The authors have included nearly all techniques developed in Arabidopsis, others recently adapted from the traditional work in crop species, and the most recent ones using Arabidopsis as a model system. Highlights include the most recent methods—transcriptomics, proteomics, and metabolomics—and their novel applications (phosphoproteomics, DNA microarray-based genotyping, high throughput metabolite profiling, and single-cell RNA).
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 | Z705055 | Artificial Enzymes | This first book on this fascinating topic is edited by one of today's most famous and internationally respected organic chemists, who has been studying enzyme mimics and artificial enzymes for more than four decades. Ronald Breslow has brought together leading scientists in this expanding area for an in-depth survey of the synthesis and design of enzyme-like catalysts.
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 | Z702625 | Bio conjugation Protocols: Strategies and Methods | Experts update the classic bioconjugation methods and introduce techniques that go beyond pure biomedical applications to include elements from organic synthesis, molecular biology, and materials science. These methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address on-chip synthesis to generate microarrays for the high-throughput analysis of RNA and DNA.
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 | B9911 | Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology | This truly unique road map to modern biochemistry is an atlas of multi-step reactions in biological systems. The book graphically represents this network of reactions with a series of chemical formulas along with two-dimensional spatial arrangements demonstrating each molecule's cellular, organic, and structural aspects. It is an encyclopedic, comprehensive, and clearly organized reference for any biochemist.
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 | Z731005 | Biochemistry 6th ed. | In the new edition of Biochemistry, instructors will see the all the hallmark features that made this a consistent bestseller for the undergraduate biochemistry course: exceptional clarity and concision, a more biological focus, cutting-edge content, and an elegant, uncluttered design. Accomplished in both the classroom and the laboratory, coauthors Jeremy Berg and John Tymoczko draw on the field’s dynamic research to illustrate its fundamental ideas.
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 | B6308 | Biochemistry of Cell Signalling | This will serve as a reliable map through the maze of cell signaling pathways and help the reader understand how malfunctions in these pathways can lead to disease. Part 1 describes the machinery of signal transduction starting with the properties of signals, receptors (including receptor activation), regulators, and the molecules that link receptor and regulator. Part 2 describes the implementation of the signaling cascades focusing on the effect on gene transcription. Part 3 studies the global cellular regulatory programs for the control of cell growth and proliferation. Part 4 considers loss of regulatory control and its consequences with respect to the molecular basis of cancer.
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 | Z701297 | Biochemistry of Signal Transduction and Regulation, 3rd ed. | This book has become the "gold standard" in the field of signal transduction and offers unmatched quality from the pen of a single author. The author succeeds in balancing comprehensive coverage with a clear and didactic style. The new edition is completely updated. 50% of the content has been rewritten to incorporate recent findings, and many illustrations have been redrawn and are now presented in full color.
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 | Z731390 | Biochemistry of Signal Transduction and Regulation, 4th ed. | This all-new edition of a best-selling text has been thoroughly updated to keep pace with the rapid progress in signal transduction research. With skill and clarity, the molecular basis of signal transduction, regulated gene expression, the cell cycle, tumorigenesis and apoptosis is made transparent for everyone with a basic knowledge in biochemistry or molecular biology. "The clear and didactic presentation makes it a textbook very useful for students and researchers not familiar with all aspects of cell regulation." (Biochemistry) "This book is actually two books: Regulation and Signal Transduction." (Drug Research)
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 | Z700444 | Biochemistry:The Chemical Reactions of Living Cells, 2nd ed., Vol. 1 | The most comprehensive textbook/reference ever to cover the chemical basis of life, the "Green Bible of Biochemistry" has been a well-respected contribution to the field for more than twenty years. The complex structures that make up cells are described in detail, along with the forces that hold them together, and the chemical reactions that allow for recognition, signaling and movement. There is ample information on the human body, its genome, and the action of muscles, eyes, and the brain. The complete set deals with the natural world, treating the metabolism of bacteria, toxins, antibiotics, specialized compounds made by plants, photosynthesis, luminescence of fireflies, among many other topics.
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 | Z703559 | Biocomputing: Computer Tools For Biologists | BioComputing: Computer Tools for Biologists contains a selection of BioTechniques' best Biocomputing papers from 1996-2002 augmented with original author updates. This comprehensive collection provides researchers with simple programs and open source code that can be used as models to study, copy, and modify for developing new computer programs. It also contains useful tools and insights into how to design better queries and interpret results more carefully, thus making more effective use of websites such as BLAST and PubMed.
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 | B5056 | Bioinformatics Basics: Applications in Biological Science and Medicine | Scientists use an extensive databank to access structural information on proteins and nucleic acids and a specialized form of software to analyze the hybridization pattern of DNA chips. The success of biotechnology and pharmaceutical industries has been a result of technical advancements in genomics, proteomics, and bioinformatics. This book describes the organization of public domain databases with a tutorial for the services described. It concentrates on three major database clusters and relevant software tools that are maintained in the US, Europe, and Japan.
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 | Z700517 | Bioinformatics for Dummies | A practical introduction to bioinformatics-computer technologies that biochemical and pharmaceutical researchers use to analyze genetic and biological data * Shows how to do sophisticated bioinformatic analysis without learning UNIX first * Helps researchers choose the right bioinformatics tool, use it effectively, and interpret the results * Guides readers to the most helpful Web resources and freely available tools * Addresses common biological questions, problems, and projects, while also providing a UNIX/Linux overview and tips on using Perl * Companion Web site contains the data sets used in the book and useful related links
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 | Z730386 | Bioinformatics for Dummies 2nd ed. | Explore the human genome and analyze DNA — without leaving your desktop! All it takes is the basics of molecular biology and the wealth of information in this friendly guide. You'll discover the online tools and useful tips you need to ask the right questions, analyze sequences, and compare results. It's a plain-English introduction to the field! Discover how to do biological research online; Use gene-centric databases; Visualize proteins in 3-D; Interpret and analyze sequences; Produce and publish results.
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 | Z703591 | Bioinformatics: A Guide to the Analysis of Genes and Proteins, 3rd ed. | Using relevant biological examples, the book provides background on and strategies for using many of the most powerful and commonly used computational approaches for biological discovery. This new edition features new chapters on genomic databases, predictive methods using RNA sequences, sequence polymorphisms, protein structure prediction, intermolecular interactions, and proteomic approaches for protein identification. It contains detailed worked examples illustrating the use of the concepts presented. Special topic boxes and appendices highlighting experimental strategies and advanced concepts are included with annotated reference lists, comprehensive lists of relevant Web resources, and an extensive glossary of commonly used terms in bioinformatics, genomics, and proteomics
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 | B8308 | Bioinformatics: From Genomes to Drugs, 2 Volume Set | Bioinformatics promises to revolutionize the process of drug discovery and development. This book provides an application-oriented overview of this technology. In addition, the state-of-the-art in bioinformatics is evaluated from a global view by introducing real application scenarios such as genome projects that require the use of a whole set of bioinformatics tools. The knowledge on bioinformatics presented enables readers to go beyond a mere push-button approach to using bioinformatics software and interpreting the data generated, but also to assess the potential and limitations of today's bioinformatics software and future challenges.
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| | Z730866 | Bioinformatics: Methods Express | The book provides the clear advice and explicit protocols that non-bioinformaticians need in order to understand what to do - and how to avoid common pitfalls. Each chapter guides you through the major databases and tools with worked examples - all accompanied by sample data files available online.Topics covered include:*data access, *sequence searches and alignments, *identification and annotation of features, *the transcriptome, *protein structure and function, *comparisons and phylogeny. This book is a comprehensive manual for all wet-bench scientists who need to use bioinformatics - from postgraduate student to principal investigator.
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 | Z651931 | Bioinformatics: Sequence and Genome Analysis, 2nd Edition | As more species' genomes are sequenced, computational analysis of these data has become increasingly important. The second edition of this widely praised textbook provides a comprehensive and critical examination of the computational methods needed for analyzing DNA, RNA, and protein data, as well as genomes. New features include chapter guides and explanatory information panels and glossary terms. New chapters cover statistical analysis of sequence alignments, computer programming for bioinformatics, and data management and mining. Practically oriented problems at the ends of chapters enhance the value of the book.
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 | Z651923 | Bioinformatics: Sequence and Genome Analysis, 2nd Edition | As more species’ genomes are sequenced, computational analysis of these data has become increasingly important. The second, entirely updated edition of this widely praised textbook provides a comprehensive and critical examination of the computational methods needed for analyzing DNA, RNA, and protein data, as well as genomes. The book has been rewritten to make it more accessible to a wider audience, including advanced undergraduate and graduate students. New features include chapter guides and explanatory information panels and glossary terms. New chapters in this second edition cover statistical analysis of sequence alignments, computer programming for bioinformatics, and data management and mining. Practically oriented problems at the ends of chapters enhance the value of the book as a teaching resource. The book also serves as an essential reference for professionals in molecular biology, pharmaceutical, and genome laboratories.
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 | B8161 | Bioinformatics: Sequence, Structure and Databanks, A Practical Approach | Covers practical important topics in the analysis of protein sequences and structures. It compares amino acid sequences to structures comparing structures to each other, and presents searching information on entire protein families as well as searching with single sequences. It shows how to set up and use the SRS molecular biology database management system. There are chapters on multiple sequence alignment and protein secondary structure prediction.
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| | Z269670 | Biologically Active Peptides: Design, Synthesis, and Utilization | Investigation into basic and advanced peptide design, synthesis, evaluation and utilization. New therapeutic approaches from experimental systems.
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 | Z730343 | Biology of Cancer | The Biology of Cancer synthesizes the findings of three decades of recent cancer research and proposes a conceptual framework from which to teach about these discoveries. It provides the necessary structure, organization, and content for a course on cancer biology for advanced undergraduates and beginning doctoral students. The book is comprehensive and offers many pedagogical features to assist teaching and learning. The text provides current information on topics such as tumor stem cells and recently introduced chemotherapeutics. State-of-the-art techniques are discussed throughout. Modern biomedical research is explored, helping readers to hone their analytical abilities and to assimilate and think clearly about complex biological processes.
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 | B4556 | Biology of Phosphoinositides | This review of current lipid signaling research places emphasis on the integration of the use of lipid signals in signal transduction and membrane trafficking. The regulation of phospholipase C, phospholipase D, phosphoinositide-3-kinases, chloride channel conductance by inositol (3,4,5,6) tetraphosphate, and of cytoskeletal protein activity by inositol lipids are covered in depth. There is specific discussion of the PH and FYVE lipid domains that allow lipids to control the movement, location, and activation-state of membrane proteins.
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| | Z288179 | Biomedical Frontiers of Fluorine Chemistry | | pricing |
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| | Z369985 | Bioorganic Chemistry: Nucleic Acids | This inaugural volume in a major new series focuses on nucleic acids from an organic chemical viewpoint. From the structure and function of DNA and RNA to chemical and enzymatic synthesis to alternative structures and structures of macromolecular complexes, this unique approach offers important insights into biology at the molecular level.
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 | Z718351 | C. elegans II | Studies of the cells and genes of the nematode C. elegans have become a cornerstone of current biology. A classic 1988 Cold Spring Harbor monograph, The Nematode Caenorhabditis elegans, described the basic genetics, anatomy and development of the organism. Now, in that authoritative tradition, comes C. elegans II -- not a second edition but a book that breaks new ground and defines the current status of the field, providing a detailed molecular explanation of how development is regulated and the nervous system specifies varied aspects of behavior. This volume is a must for any investigator doing worm studies but it has been written and rigorously edited to illuminate for a wider community of investigators in cell and molecular biology who should know how new knowledge of C. elegans relates to their own specialty.
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 | Z340693 | C. elegans: A Practical Approach | Caenorhabditis elegans is currently the only multicellular animal to have its entire genome sequenced. This book provides all the essential background information required for the use of C. elegans as a model system and includes information on how to use the genome sequence.
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 | Z373710 | Calreticulin | | pricing |
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 | Z358851 | Carbohydrate Analysis: A Practical Approach, 2nd ed. | This book is the result of the need for a handbook of laboratory protocols in the complex field of carbohydrate analysis. Chapters are arranged on the basis of carbohydrate moiety to facilitate choice of analytical method for specific applications. New methods (supercritical fluid chromatography, capillary electrophoresis and NMR) have been added, while less used methods have been deleted.
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 | Z705098 | Carrier-bound Immobilized Enzymes: Principles, Applications and Design | The first systematic overview of this key technique since the early 1990s, this authoritative reference is the only handbook available to include all recent developments. The author draws on his wide-ranging experience in both academia and industry to systematically cover all types of enzyme immobilization methods, including such novel techniques as site-specific immobilization, imprinting and immobilization in organic solvents. Throughout, a careful review of materials and techniques for the preparation of functional immobilized enzymes that benefits both developers and users of carrier-bound enzymes.
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 | C3487 | Cell Adhesion | Cell adhesion to particular ligands can affect cytoskeletal organization and cell polarity, cell proliferation, and gene expression. This book provides a discussion of the structure and function of the major classes of cell adhestion molecules: integrins, cadherins, selectins, heparan sulfate proteoglycans, the immunoglobulin superfamily, the ADAMs proteins, and transmembrane protein tyrosine phosphatases. Their role in processes such as cell mediated immunity, development and disease are discussed. The molecular organization and function of junctional complexes are also examined.
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 | Z705578 | Cell Biology Protocols | As a modern composite scientific discipline, cell biology has expanded rapidly in recent years. Cell biologists now require a wide range of techniques, including those of analytical biochemistry and microscopy in all its diverse forms. These are often used alongside the techniques of molecular biology and molecular genetics. This book contains numerous useful protocols, covering light and electron microscopy, cell culture, cell separation, subcellular fractionation, organelle and membrane isolation, and the use of in vitro reassembly systems in cell biology. Many of these protocols feature helpful notes and safety information for practical application. The format favors easy use at the bench with space for notes and important safety information. An appendix contains essential analytical information that will prove invaluable.
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 | Z704865 | Cell Biology: A Laboratory Handbook, 3rd ed. 4 volume set | This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems. This thoroughly updated edition contains 165 new articles. Topics covered include:
Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1); Organelle and Cellular Structures, Assays (Volume 2); Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3); Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4)
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 | C9862 | Cell Physiology Sourcebook: A Molecular Approach, 3rd ed. | This authoritative book gathers together a broad range of ideas and topics. It provides clear, concise, and comprehensive coverage of cellular physiology from fundamental concepts to more advanced topics and contains substantial new material. The book includes chapters on sensory transduction, the physiology of protozoa and bacteria, the regulation of cell division, and programmed cell death. Features include eight new chapters on such topics as membrane structure, intracellular chloride regulation, transport, sensory receptors, pressure, and olfactory/taste receptors; broad coverage of both animal and plant cells.
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 | Z703265 | Cell Surface Proteases | This book provides a comprehensive overview of these important enzymes that catalyze the hydrolysis of a protein as it degrades to a simpler substance. In the 1990s, an explosion of new discoveries shed light on the role of cell surface proteases and extended it beyond degradation of extracellular matrix components to include its influence on growth factors, cell signaling, and other cellular events. This volume unites the scientific literature from across disciplines and teases out unified themes of interactions between cell surface proteases and interconnecting cell surface-related systems, including integrins and other adhesion molecules.
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| | Z364320 | Cell and Molecular Biology: Essential Data Series | This handy reference brings together a wide range of core information needed by all researchers in the biomolecular sciences and additional selected information from the areas of biochemistry, cell biology, genetics and molecular biology.
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 | C0613 | Cell-Penetrating Peptides: Processes and Applications | This book fills the need for a comprehensive update concerning cell-penetrating peptides (CPPs), to which the drug molecule can attach and pass through the cell membrane. It covers classes of cell-penetrating peptides; mechanisms of cell penetration, interactions of cell-penetrating peptides with plasma membranes, and lipid models; and applications of cell-penetrating peptides. These divisions cover information from basic biochemistry to clinical application, including a description of the general rules for cellular uptake of the transport peptides. This overview will lead to better designed novel transport peptides to achieve successful application of CPPs.
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 | Z730173 | Cell: A Molecular Approach, 4th ed. | This book provides students with not only the most current information, but also with a conceptual framework and an introduction to the experimental nature of contemporary research. The 4th edition includes the role of microRNAs in regulation of gene expression; Expanded discussions of genomics, proteomics, and signaling networks; Translational regulation and the mTOR signaling networks; Roles of specialized lipid domains in cell surface processes; Advances in stem cell biology; Oncogenes and the rational design of new anticancer drugs. Key Features of the Fourth Edition are three additional chapters, including "Cell Death and Cell Renewal"; Major new sections, including:; Proteomics: Large-Scale Analysis of Cell Proteins; Bioinformatics and Systems Biology; Cell-Matrix Interactions; Signaling Networks.
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 | C9853 | Chaperonin Protocols | This is a collection of readily reproducible protocols for the study of chaperonins, intracellular proteins critical to many biological processes. It focuses on the purification of chaperonins from different species along with their corresponding cofactors, and on chaperonin activity assays for in vivo as well as in vitro work.
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| | Z362352 | Communication within Animal Cells | A clearly written textbook with many illustrations for students in advanced undergraduate and graduate courses. It puts into perspective a large body of knowledge about intracellular signaling systems.
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 | Z704970 | Cytochrome P450 Protocols, 2nd ed. | For this edition, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.
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| | Z700347 | Cytochrome P450, Part C Methods in Enzymology Vol 357 | The exponential growth of known P450s over the past few yearsis a consequence of accelerated activity in genomics involving both eukaryotes and prokaryotes. Many of the articles in this volume provide insights into how function of P450s can be examined. Genomics captures a complete section and extensive details on determination or structure/mechanism and transcriptional regulation of P450 gene expression by nuclear proteins are found. Key features of this volume are Human Genomics and Genetics; Structure and Mechanism; Regulation of Expression; Metabolism; Invertebrate P450s.
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 | D9313 | DNA Topoisomerase Protocols | This is a collection of protocols for investigating DNA structure, topology, and DNA topoisomerase function. These methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. Protocols detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases.
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 | Z730017 | Deoxynucleoside Analogs in Cancer Therapy | This book expertly summarizes the current status of development and application of deoxynucleoside analogs. Organized into several sections, the first part covers general aspects of drug uptake and metabolism and explains how novel technology has enabled a rapid expansion of this field. The second part is concerned with a number of specific drugs including cytarabine, gemcitabine, troxacitabine, clofarabine and ara-G. The final section covers pharmacokinetics, prodrugs, and specific applications such as radiosensitization, gene therapy, and the use of deoxynucleoside analogs as tracers. Throughout the book, the focus is on novel aspects of deoxynucleoside analogs in the clinical context, as well as on unexpected targets of these compounds, such as their specific activity against cell cycle-dependent kinases or oncogenes.
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 | D8063 | Developmental Biology Protocols Volume 1 | Techniques for establishing and characterizing experimental model systems, for study of developmental patterns and morphogenesis, and for examination of embryo structure and function
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 | D8188 | Developmental Biology Protocols Volume 2 | Methods for studying organogenesis, analysis of abnormal development and teratology, screening and mapping of novel genes and mutations, and application of transgenesis
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 | D8313 | Developmental Biology Protocols Volume 3 | Techniques for manipulation of developmental gene expression and function, analysis of gene expression, characterization of tissue morphogenesis and development, in vitro study of differentiation and development, and genetic analysis of developmental models of diseases.
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 | Z702900 | Dictionary of Bioinformatics and Computational Biology | Recent developments have produced a wealth of experimental data on sequences and three-dimensional structures of biological macromolecules. As a result, the employment of various computational methods of analysis to obtain useful information is now a major new discipline: bioinformatics. This book provides a vital reference for important terms, offering a convenient summary of the core knowledge. With concise and accurate definitions of over 600 words, phrases, and concepts, this volume offers thorough A-Z coverage, lists references and Internet links, incorporates cross-referencing throughout, and features a comprehensive index of topics.
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 | Z518719 | Dictionary of Carbohydrates | Provides chemical, physical, and structural data on 20,000 carbohydrates together with selected literature references. Features easy-to-use text and substructure search software. This electronic version of the renowned Chapman and Hall dictionary of Carbohydrates offers complete coverage of:
• Parent monosaccharides together with a full range of their derivatives, e.g., deoxy, aminodeoxy and halogenodeoxy sugars
• Disaccharides and their derivatives
• Oligosaccharides, polysaccharides, and nucleosides
• Glycoside antibiotics and other structurally unusual glycosides
• Key carbohydrates used in modern synthetic methodology
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 | D0687 | Dictionary of Cell & Molecular Biology, 3rd ed. | This new edition includes 2000 new entries bringing the total to 7000 entries. Obsolete terms have been dropped and old ones revised. There is wider coverage of relevant molecular and neurobiological terms. Each entry has short, clear definitions and the presentation of certain information is in tabular format for clearer and easier reference.
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 | Z701319 | Directed Enzyme Evolution: Screening and Selection Methods | Practitioners from leading laboratories describe their best screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes. The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens. Described in detail, these methods will enable researchers to design and implement directed evolution strategies for the engineering of novel proteins. It describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function. An accompanying volume is Directed Evolution Library Creation: Methods and Protocols. (see Z70,121-1)
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 | Z701211 | Directed Evolution Library Creation: Methods and Protocols | A compendium of cutting-edge protocols for the generation of molecular diversity. These protocols include reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologus recombination, and for constructing in vivo libraries in bacteria and yeast. This volume also describes ways to analyze libraries, particularly those made by recombination. This volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. An accomapnying volume is Directed Enzyme Evolution: Screening and Selection Methods (see Z70,131-9)
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 | D4566 | Directed Molecular Evolution of Proteins, or How to Improve Enzymes for Biocatalysis | Optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio- and enantioselectivity. This book focuses on the directed evolution of proteins as a method for designing enzymes showing new substrate specificities. It includes a repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity.
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 | D4191 | Discovering Genomics, Proteomics, and Bioinformatics | This is the first textbook which combines integrated web exercises with a problem solving approach to train students in basic hands-on genomic analysis. The authors present global problems, then provide the tools of genomic analysis to help students dissect the answer. Microarray images and all art and figures from the text are available on the free CD-ROM. The Discovering Genomics, Proteomics, and Bioinformatics Companion Website www.geneticsplace.com helps one learn about cutting-edge research and visualize complex processes.
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 | D8561 | Drosophila Protocols | This exceptional laboratory manual describes procedures used for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation and rigorously edited for clarity and conciseness. The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development. Each method is introduced and illustrated with figures, tables, illustrations, and examples of data obtainable.
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 | Z651958 | Drosophila: A Laboratory Handbook, 2nd Edition | The second edition of the highly successful grey book Drosophila: A Laboratory Handbook, first published in 1989, has been completely updated to reflect advances in techniques and knowledge. The original format is maintained, providing a handy reference guide to most aspects of the biology of Drosophila. Each of the thirty-six chapters summarizes the present state of the subject, written with a historical perspective, but with an emphasis on the practical use of genetic and other methods. Extensively referenced.
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 | Z718270 | Early Development of Xenopus laevis: A Laboratory Manual | Xenopus embryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopus development has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation.
This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere.
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 | Z718572 | Early Development of Xenopus laevis: A Laboratory Manual | Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopus development has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation.
This manual contains a comprehensive collection of protocols for the study of early development in Xenopus embryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere.
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 | Z378151 | Endothelins in Biology and Medicine | The latest findings on function, effects and characteristics of endothelins (ETs), meticulously researched and thoroughly reviewed. Five sections cover ET receptors, synthesis and degradation, pathophysiology, structure-activity relationships, and clinical studies. Almost 2,000 citations to current literature put further resources at your fintertips.
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 | Z704962 | Enzyme Assays: High-throughput Screening, Genetic Selection and Fingerprinting | Nowadays, the characterization of enzymes using simple assays is a key technology for finding the optimum enzymes for, among others, catalysis in enzymology and drug discovery. Thus, enzymes have become an everyday tool not only for biologists, but also for chemists, and the number of applications is now overwhelming. This book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years.
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| | Z411256 | Enzyme Chemistry: Impact and Applications, 3rd ed. | The previous editions of this book established it firmly as an invaluable starting point and concise reference source for chemists, biochemists, microbiologists and others seeking an overview of enzyme chemistry and a gateway to the surrounding literature. This new edition covers these advances in understanding while retaining the basic structure which has proved so effective in earlier years. The aim remains the same: to provide those graduates with some knowledge of chemistry but a limited knowledge of enzymes, with a one-stop access point to this strategically critical field.
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 | Z700355 | Enzyme Kinetics and Mechanism, Part F: Detection and Characterization of Enzyme Reaction, Methods in Enzymology Vol 354 | In this volume of Methods in Enzymology, every chapter is devoted to the task of detecting and characterizing enzyme reaction intermediates. This volume contains detailed descriptions of a broad range of experimental approaches that are applied to a diversified group of enzymes and reaction types. Key features of this volume include Spectroscopic Detection of Reaction Intermediates; Isotopic and Kinetic Detection of Reaction Intermediates; Chemical Trapping and Inhibitor Methods for Detecting Reaction Intermediates.
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 | Z700363 | Enzyme Kinetics: A Modern Approach | This book provides a guide to evaluate enzyme kinetics, using common software packages to perform enzymatic analyses. The treatment of enzyme kinetics here is radically different. Marangoni stresses an understanding of how researchers arrive at models, what the models' limitations are, and how they can be used in ways to analyze enzyme kinetic data. Computers make linear transformations of models unnecessary, and this book does away with them, advancing the use of nonlinear regression techniques. Marangoni develops new ways to carry out analyses of enzyme kinetic data, particularly in the study of pH effects on catalytic activity and multisubstrate enzymes.
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 | E9017 | Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis, 2nd ed | This book explains the structural complexities of proteins and enzymes and the mechanisms by which enzymes perform their catalytic functions. New features include a new chapter on protein-ligand binding equilibria, expanded coverage of chemical mechanisms in enzyme catalysis and experimental measurements of enzyme activity, updated discussions of enzyme inhibitors and multiple substrate reactions, and coverage of current practical applications to the study of enzymology.
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 | Z702889 | Essential Cell Biology 2nd ed. | The second edition contains basic, core knowledge about how cells work. The text and figures have been prepared to be easy-to-follow, accurate, clear and engaging for the introductory student. Each section follows logically from the previous one, telling a story, rather than being a collection of facts. Questions integrated throughout each chapter encourage the reader to pause, think about what they have read, and attempt to apply the new knowledge in ways that test their understanding. Based on user feedback, this edition now offers increased coverage of genetics and more experimental background.
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 | Z701092 | Essential Cell Biology: A Practical Approach 2 volume set | The challenge was not to compile a collection of all the techniques available in cell biology, but to identify those methods that are key to research progress and most relevant to researchers new to the topic.
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| Z700924 | Essential Cell Biology: A Practical Approach Volume 1: Cell Structure | Volume 1 focuses on techniques for studying cell structure while volume 2 concentrates on understanding how the cell functions. Volume 1 details the essential background information and protocols for observing and understanding cell morphology and cell structure, including, for example, investigations of nucleic acids, lipids, and the cytoskeleton.
The challenge was not to compile a collection of all the techniques available in cell biology, but to identify those methods that are key to research progress and most relevant to researchers new to the topic.
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| Z700932 | Essential Cell Biology: A Practical Approach Volume 2: Cell Function | Volume 2 concentrates on understanding how the cell functions while Volume 1 focuses on techniques for studying cell structure. Volume 2 details the essential background information and protocols for investigating cell function, including, for example, investigations of cellular transport, receptors and gene expression.
The challenge was not to compile a collection of all the techniques available in cell biology, but to identify those methods that are key to research progress and most relevant to researchers new to the topic.
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 | Z718882 | Essentials from Cells: A Laboratory Manual (Laboratory Essentials on CD) | Published in 1998, the three-volume lab manual Cells contained a comprehensive collection of techniques for studying cellular structure and function. This new CD-ROM,Essentials from Cells: A Laboratory Manual, presents a selection from the book of methods with proven reliability that every investigator working with cellular systems should have available. Essentials from Cells: A Laboratory Manual provides precisely crafted, step-by-step protocols and essential background details in a readily accessible, searchable electronic format.
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 | E7154 | Essentials of Genomics and Bioinformatics | Genomics is a new discipline that is revolutionizing the way biological and medical research is done. The human genome is almost completely sequenced, which will help to accelerate research and development in Genomics even more. The chapters in this book capture the rapidly evolving field. Overviews of nucleic acid technologies, proteomics and bioinformatics are followed by chapters on applications, including human health, agriculture and comparative genomics. The book also includes two chapters on legal and ethical issues of Genomics, a topic that is becoming more and more important.
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 | E7279 | Essentials of Glycobiology | Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. They participate in activities as diverse as protein folding, leukocyte physiology, and microbial pathogenesis. Genetic defects in their assembly are at the root of a growing list of human diseases. This book is a gateway to the understanding of glycans, and is the first textbook approach to describing their biogenesis and function. Written and edited by glycobiologists, this volume will be invaluable for those who need a complete, concise introduction to this field.
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 | Z718459 | Essentials of Glycobiology | Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. They participate in activities as diverse as protein folding, leukocyte physiology, and microbial pathogenesis. Genetic defects in their assembly are at the root of a growing list of human diseases. Essentials of Glycobiology is an ideal gateway to the understanding of glycans, and is the first textbook approach to describing their biogenesis and function. Written and edited by glycobiologists with experience in both teaching and research, this volume will be invaluable for students and established investigators in developmental biology, cell biology, neuroscience, immunology, and biochemistry, who need a complete yet concise introduction to this burgeoning field.
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 | Z567159 | Essentials of Glycobiology, 2nd edition | The sugar chains of cells-known collectively as glycans-play a variety of impressive, critical, and often surprising roles in biological systems. Glycobiology is the study of the roles of glycans in the growth and development, function, and survival of an organism. Glyco-related processes, described in vivid detail in the text, have become increasingly significant in many areas of basic research as well as biomedicine and biotechnology. This new edition of Essentials of Glycobiology covers the general principles and describes the structure and biosynthesis, diversity, and function of glycans and their relevance to both normal physiologic processes and human disease. Several new chapters present significant advances that have occurred since the publication of the first edition. Three sections of note describe organismal diversity, advances in our understanding of disease states and related therapeutic applications, and the genomic view of glycobiology. “Glycomics,” analogous to genomics and proteomics, is the systematic study of all glycan structures of a given cell type or organism and paves the way for a more thorough understanding of the functions of these ubiquitous molecules. The first edition of Essentials of Glycobiology represented also a notable experiment in publishing, as it became one of the first electronic textbooks. And, now, in recognition of its wide audience and the changing ways in which researchers and students learn and access information, the new edition of Essentials will be made available online simultaneously with the print edition. This novel experiment is the result of the collaborative efforts of the Cold Spring Harbor Laboratory Press, the National Center for Biotechnology Information, and the editors of the book. Written and edited by glycobiologists with experience in teaching and in research, this volume will be an invaluable resource, both for students and for established investigators in fields such as developmental biology, cell biology, neuroscience, immunology, and biochemistry who require a complete yet concise introduction to this burgeoning field.
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 | Z730491 | Experimental Design for Biologists | Experimental Design for Biologists explains how to establish the framework for an experimental project, how to set up a system, design experiments within that system, and how to determine and use the correct set of controls. Separate chapters are devoted to negative controls, positive controls, and other categories of controls that are perhaps less recognized, such as "assumption controls," and "experimentalist controls." Furthermore, there are sections on establishing the experimental system, which include performing critical "system controls." Should all experimental plans be hypothesis-driven? Is a question/answer approach more appropriate? What was the hypothesis behind the Human Genome Project? The answers to these kinds of questions can be found in this book. It provides compelling lessons in framing an experimental question, establishing a validated system to answer the question, and deriving verifiable models from experimental data.
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 | E0641 | Extracellular Matrix Protocols | Leading bench-top experimentalists present state-of-the-art methods for probing the extracellular matrix (ECM), its mechanisms of assembly and how it influences cell behavior. These highly detailed biochemical, biophysical, genetic, molecular biological, and cell biological techniques have been optimized for study of ECM structure and assembly, mechanisms of cell–ECM interactions, and molecular genetics of ECM proteins and defects in human disease.
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| | Z306231 | Extracellular Matrix: AÂ Practical Approach | The importance of the extracellular matrix in maintaining the structural integrity of organisms, in regulating the normal biology of a cell, and in the development and progression of disease, has established this area as a major focus of contemporary research. This book is a compendium of the biochemical, immunological, and molecular biological techniques and approaches used to study both extracellular matrix macromolecules and their function in vertebrates.
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| | Z369721 | Fatty Acid and Lipid Chemistry | This book is written for those who, with some knowledge of chemistry and biochemistry, need to know more about the nature of lipids and fatty acids. Nomenclature, structure, biosyntheses, chemical syntheses, major sources, processing, analytical procedures, and physical properties are among the topics discussed.
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| | Z272876 | Fluorine-Containing Amino Acids | More than 700 amino acids (AAs) have been found in nature and their number is continually increasing. Internationally renowned scientists critically examine the chemistry of fluorine-containing amino acids (FAAs) and the existing knowledge regarding their biological properties. A synthesis of FAAs by methods of acid, organofluorine and organometallic chemistry; enzymatic and chromatographic resolution of racemic FAAs; biological characteristics of FAAs and their applications are among the subjects covered.
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 | Z340731 | Fmoc Solid Phase Peptide Synthesis: A Practical Approach | This new volume covers essential procedures for production of linear peptides and more advanced techniques for preparing cyclic, side-chain modified, phospho- and glycopeptides. Many other methods have been included: convergent peptide synthesis; peptide-protein conjugation; chemoselective ligation; and chemoselective purification. The difficult preparation of cysteine and methionine-containing peptides is covered, as well as methods for overcoming aggregation during peptide chain assembly.
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 | F2799 | Free Radical and Antioxidant Protocols | Seventy international experts join forces to present step-by-step instructions for a wide range of cutting-edge assays to study primary and secondary oxidative stress. Techniques for assays on free radicals, antioxidant enzymes, antioxidant vitamins and nonvitamins, trace metals, and cellular/membrane transport are included. They take advantage of new instrumentation such as probes, photon counting, chemiluminescence and caged compounds with an emphasis on HPLC. The methods are adaptable to a wide range of applications.
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 | Z731099 | Free Radicals in Biology and Medicine 4th ed. | This edition has been comprehensively rewritten and updated (over 80% of the text is new), while maintaining the clarity of its predecessor. There is expanded coverage of isoprostanes and related compounds, mechanisms of oxidative damage to DNA and proteins (and the repair of such damage), the free radical theory of aging and the roles played by reactive species in signal transduction, cell death, human reproduction, and other important biological events. Greater emphasis has also been placed on the methods available to measure reactive species and oxidative damage (and their potential pitfalls), as well as the importance of antioxidants in the human diet.
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 | F3049 | Functional Genomics: A Practical Approach | The complete genome of many organisms is available and there is a demand for techniques to utilize these resources. This book provides a comprehensive treatment of the range of methods available for gene and protein expression profiling in a variety of systems. These include large-scale methods, such as cDNA microarrays, serial analysis of gene expression and proteomics, and also methods suitable for non-specialist laboratories, such as differential display and suppression subtraction hybridization.
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 | Z705241 | Functional Lipidomics | Systematic analysis of the total lipid structure of a cell or organism, the lipodome, can reveal novel avenues of therapeutic intervention and diagnosis. This analysis is best modeled after the lessons learned from proteomics. It introduces lipidomics, explores the current state of the field, and provides a comprehensive background for future work. The first book dedicated to the topic, first defines and introduces the field before delving deeply into the methods, processes, analyses, and research aspects. The book uncovers the biosynthesis, metabolism, and function of fatty acids, acylglyerols, glycerophospholipids, sphingolipids, terpenes, and lipids that complex with proteins. Contributors elaborate upon cutting-edge approaches to lipid analysis, and the therapeutic significance of targeting proteins involved with the lipid signaling pathway.
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 | G3543 | GTPase Protocols: The Ras Superfamily | This is a collection of techniques to probe the function of these ubiquitous proteins both in vitro and in vivo. Each technique includes step-by-step instructions, tips on avoiding pitfalls and troubleshooting, and ancillary notes explaining how to adapt each procedure in the event of problems. The methods cover the spectrum of core techniques required for the five major GTPase subfamilies (Ras, Rho, Rab, Arf, and Ran) and permit a diversity of applications ranging from structural studies on a GTPase to real time in vivo analysis. This book illuminates the techniques used to study this family of proteins.
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 | G9403 | GTPases | GTPases are proteins that act as molecular switches to control biochemical pathways within a cell. This book provides a complete guide to the properties and cellular roles of all the major families of GTPases, including the heterotrimeric G proteins and the small GTPases Ras, Rho, Rab, Arf and Ran. These proteins have caught the attention of those studying control of proliferation, differentiation, cell polarity, cell movement and vesicle and protein trafficking.
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 | Z651974 | Gastrulation: From Cells to Embryo | Gastrulation is the complex process of cell differentiation, following the division of a fertilised embryo, during which tissue layers are formed and the overall body plan is established. This book is the definitive guide to this vitally important period in embryonic development, providing authoritative and up to date information that includes the first comprehensive interspecies comparison, cell movements and patterning events, the roles of individual genes and gene families, and the evolution of gastrulation.
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 | Z373974 | Gene Isolation and Mapping Protocols: Methods in Molecular Biology, Vol. 68 | A collection of the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using data base analyses. Also, numerous time-saving techniques and informative explanations about certain steps are included.
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| | Z306177 | Gene Probes 1 | Over 85 key protocols for the synthesis, labeling, detection, and quantification of DNA, RNA, oligodeoxynucleotide, and antibody probes are provided. It brings together key practical advice and step-by-step protocols for all of the major methods of probe labeling and detection.
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 | Z306185 | Gene Probes 2 | Over 100 protocols for using DNA, RNA, oligodeoxynucleotide, and antibody probes in screening gene libraries, mapping, gene structure determination, analysis of gene expression, and mutational studies, are presented.
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 | G1793 | Gene Probes: Principles and Protocols | Volume 179 of the Methods in Molecular Biology series contains an outstanding collection of time-tested protocols for designing and using genes probes in wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions
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 | Z730327 | Gene Regulation 5th ed. | Gene regulation is an essential process in the development and maintenance of a healthy body. This Fifth Edition provides a clear, up-to-date description of gene regulation in eukaryotes, distilling the vast and complex primary literature into a concise overview. For this edition, in addition to updating of existing material, sections on important methodologies have been expanded, and a new section included on regulation by small interfering RNAs. More detail has been added on the role of multi-protein complexes in transcriptional activation, and to the discussion of the regulation of transcription factor activity by specific modifications, to include acetylation and ubiquitination, as well as phosphorylation. The final chapter on gene regulation and human disease now includes additional novel examples, and a new section on the therapeutic potential of gene regulation.
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 | Z340790 | Gene Targeting: A Practical Approach, 2nd Edition | This updated version includes the major new techniques and tried-and-tested practical protocols. It concentrates on gene targeting in mouse ES cells, but the techniques can be adapted to applications in tissue culture, including human cells.
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 | G9291 | Gene Therapy: Therapeutic Mechanisms and Strategies | This reference covers all aspects of gene therapy, including viral and nonviral delivery systems, targeted diseases, regulatory issues, and FDA requirements. It investigates genetic bullets to block HIV-1 replication and genetic guns to deliver antiviral agents. It examines donor cells, vector systems, and functional genes; defines extra- and intracellular pathways and barriers for gene delivery; addresses the interaction of micelles and liposomes with nucleic acids; describes the formulations, pharmacology, and toxicology of oligonucleotides; considers RNA catalysis and decoy RNAs; and outlines hematopoietic stem cell procurement.
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 | G8416 | Gene Transfer Methods: Introducing DNA Into Living Cells and Organisms | A critical aspect of the molecular revolution is that DNA frequently must be re-introduced into living cells to determine activity. The goal of this book is to compile a core set of numerous DNA transfer methods within a single volume. Chapters discuss DNA transfer into cells of bacterial, fungal, and mammalian origin, as well as into the germlines of flies, mice, and plants. Each chapter includes supplementary information such as common problems and specific troubleshooting suggestions. Progress and hurdles in the field of human gene therapy are presented.
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 | Z730033 | Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual | Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. This book provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response.
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 | Z702617 | Gene and Cell Therapy: Therapeutic Mechanisms and Strategies, 2nd ed. | This edition offers current data on delivery systems and therapeutic strategies, gene expression and detection, and disease targets and therapeutic strategies. It presents results and prospects in gene therapy clinical trials, other cell-based therapies and trials, and regulatory issues. Topics include optimization of non-viral gene therapeutics, gene therapy for cancer, and DNA vaccines.
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 | Z702420 | Genes VIII | Two decades ago Benjamin Lewin's Genes revolutionized the teaching of molecular biology and molecular genetics by introducing a unified approach to bacteria and higher organisms. Genes has remained at the cutting edge of molecular biology, covering gene structure, organization, and expression. Originally the text opened with the genetic code and worked toward genome structure. Genes VIII changed the approach to begin with the sequence of the human and other genomes and starts with complete coverage of recent advances in genomics. The coverage of genomics is then integrated throughout the text.
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 | Z379662 | Glycoanalysis Protocols (2nd ed.): Methods in Molecular Biology, Vol. 76 | A compendium of structure elucidation techniques for scientists in the areas of glycoproteins, lipoproteins, proteoglycans, and complex carbohydrates. Includes oligosaccharide profiling, macromolecular structural analysis, and microorganism structure determination. Special attention is given to analysis of recombinant glycoproteins as therapeutics.
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 | Z730130 | Glycobiology Protocols | A panel of experts reviews the importance of glycobiology. This book highlights important methodological progress that has been made in the field of glycobiology, helping scientists to answer specific questions on glycoprotein structures, on the biosynthesis of glycoconjugates, and on the functions of lipid- or protein-bound carbohydrates. This book contains specific methods for the analysis of the structures or functions of glycoconjugates, as well as of glycosyltransferases and glycosidases involved in the biosynthesis of glycans. The methods described for specific systems can be modified for investigations of similar biomolecules and tissues or cell types. This book provides researchers with a solid basis for studying the many aspects of glycobiology with state-of-the-art methods.
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 | Z379972 | Guide to the Polyamines | A comprehensive survey of the physiological and structural functions of biological polyamines. Both normal and pathological functions are covered, the latter represented especially by cancer, viral infection, and protozoan parasitism. There is extensive coverage of the role of polyamines in polynucleotide organization.
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| | Z364215 | Guidebook to the Secretory Pathway | Addresses many aspects of the secretory pathway with almost two hundred entries. Provides succinct, up-to-date information with short bibliographies regarding cell-cycle control and membrane traffic.
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 | Z703087 | Handbook of ATPases: Biochemistry, Cell Biology, Pathophysiology | ATP-dependent active ion transport enables cells to regulate their pH value and to control their ion composition. The reverse process, transforming an ion imbalance into chemical energy, drives mitochondrial and chloroplast ATP synthesis. The mediators of these fundamental processes are ion-motive ATPases, highly conserved enzymes that play key roles in cell physiology from bacteria to man. This handbook summarizes recent knowledge about the molecular mechanism of ATPases, relating this information to the physiology and pathophysiology of ion transport, mitochondrial function, vesicle transport and lysosomal acidification. All important P-type, F-type and V-type ATPases are treated systematically, complemented by a special section on the cell biology and physiology of acidic compartments.
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 | Z702684 | Handbook of Proteolytic Enzymes, 2nd ed. | Extensively revised and updated, the new edition of this highly regarded handbook is an essential reference for biochemists, biotechnologists and molecular biologists. Edited by world-renowned experts in the field, this comprehensive work provides detailed information on all known proteolytic enzymes to date. This two-volume set unveils new developments on proteolytic enzymes which are being investigatedin pharmaceutical research for such diseases as HIV, Hepatitis C, and the common cold. Volume I covers aspartic and metallo petidases while Volume II examines peptidases of cysteine, serine, threonine and unknown catalytic type. A CD-ROM accompanies the book containing fully searchable text, specialised scissile bond searches, 3-D color structures and much more.
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 | Z705438 | Histone Deacetylases: Transcriptional Regulation and Other Cellular Functions | A panel of leading investigators summarizes and synthesizes the new discoveries in the rapidly evolving field of histone acetylation as a key regulatory mechanism for gene expression. The authors describe what has been learned about these proteins, including the identification of the enzymes, the elucidation of the enzymatic mechanisms of action, and the identification of their substrates and their partners. They also review the structures that have been solved for a number of enzymes—both alone and in complex with small molecule inhibitors—and the biological roles of the several histone deacetylases (HDAC) genes that have been knocked out in mice.
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 | Z705527 | Immobilization of Enzymes and Cells, 2nd ed. | The continuing rapid progress in work designed to improve the functional properties of enzymes and cells as industrial catalysts has led to this updated edition. This edition contains new and simplified protocols useful for industrial applications, novel techniques that will prove useful now or in the near future, and protocols for the prepapartion of immobilized derivatives suitable for a wide variety of nonconventional reaction media. The authors also offer tools for the development of new immobilization techniques, methods for preparing immobilized derivatives for therapeutic and industrial uses, and new chemical reactors designed to overcome the limitations of immobilized derivatives. The emphasis is on improving enzyme and cell properties via very simple immobilization protocols along with the development of new and better methods.
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| | Z375543 | Immobilization of Enzymes and Cells: Methods in Biotechnology 1 | | pricing |
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| | B9053 | Immobilized Biomolecules in Analysis: A Practical Approach | An important new technology, biosensors are made from biomolecules or cells which are immobilized, but retain their functionality. This book is an up-to-date collection of reliable methods for immobilizing biomolecules. It includes techniques for measuring the functionality of biomolecules.
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| | I9406 | Instant Notes in Biochemistry, 2nd ed. | This is up-to-date edition of the best selling "Instant Notes in Biochemistry," providing a valuable revision and information source for all undergraduates taking biochemistry courses. This best-selling Instant Notes book has now been thoroughly updated and reorganized for improved clarity. Many topics have been completely rewritten and new topics on RNA synthesis and processing, and thermodynamics have been added. Remaining concise yet comprehensive, Instant Notes in Biochemistry 2/e continues to provide easy access to the core information. The format remains ideal for learning and rapid review
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| | Z243418 | Introduction To Peptide Chemistry | This concise introduction to peptide chemistry serves as a step-by-step guide, emphasizing the principles of peptide chemistry, with its strong links to physical and organic chemistry and biochemistry. Intended primarily for advanced undergraduates, it reflects the increasing importance of peptide chemistry, particularly in relation to solid-phase synthesis, gene sequencing and x-ray crystallography. Chapters cover amino acids, purification and isolation, amino acid analysis, sequencing and synthesis. A concluding chapter discusses the structure of LH-RH.
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 | Z700525 | Introduction to Bioinformatics: A Theoretical and Practical Approach | The first section of this book describes basic cellular structure and the decoding of the genome. Second section covers regulation of genomes and molecular genetic basis of disease as a consequence of genetic replication. Third section demystifies the Unix Operating System and gives examples of command line sequence analyses. Fourth section provides information on management and analysis of DNA sequencing projects, along with a review of how DNA can be modeled as a statistical series of patterns. It follows with a discussion of the various genome databases. Included is a CD-ROM containing valuable software programs including BioDiscovery, ClustalX, Ensembl, MicroAnalyser, Staden Sequence Analysis Package, Tree View and others.
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 | Z718610 | Landmark Papers in Cell Biology: Selected Research Articles Celebrating Forty Years of The American Society for Cell Biology | Spectacular advances are being made in our understanding of cells, from the beautiful intricacy of their structure to the incredible complexity of the biochemical reactions and pathways that regulate their behavior. Published to mark the 40th anniversary of The American Society for Cell Biology, Landmark Papers in Cell Biology contains reprints of 42 seminal papers that illustrate the field’s great advances along with brief commentaries that place the papers in historical and thematic context.
Highlighting advances in cell biology since the founding of the Society in 1960, the papers - all studies of eukaryotes - were carefully screened and chosen for their significance in the field by eminent cell biologists Joseph Gall and Richard McIntosh. The articles in this timely reference are grouped according to the following themes:
Genome organization and replication
Transcription
Nuclear envelope and nuclear import
Mitosis and cell cycle control
Cell membrane and extracellular matrix
Protein synthesis and membrane traffic
Cytoskeleton
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| | Z718807 | Landmark Papers in Yeast Biology | The yeasts have been important experimental organisms for more than 50 years. This volume contains introductions to over 60 selected papers, in sections that describe the process of discovery and the context and significance of the research described in the papers. The papers referenced include early classics as well as recent advances in areas such as signal transduction, membrane trafficking, protein turnover, and genomics. This book is designed as a guide for a literature–based course.
The Landmarks Web Site: Accompanying the book, the Landmarks Web site lists the selected papers, along with links to their abstracts in PubMed and to the journals' home pages, where the full text will be available if the readers or their institutions have appropriate subscriptions or if the journals have released their back issues free. The Landmarks Web site is free to access but will require registration.
THIS BOOK WILL PUBLISH IN THE FUTURE. SIGMA-ALDRICH CAN ACCEPT ORDERS NOW FOR FULFILLMENT WHEN PUBLISHED.
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 | L7289 | Lateral DNA Transfer: Mechanisms and Consequences | This book is about mobile genes - the transfer of DNA between unrelated cells. It discusses the machinery of gene transfer and its wide-ranging biological and health consequences. Mobile DNA makes possible the development of antibiotic resistance in microbes, the conversion of harmless to pathogenic bacteria, and the triggering of cancerous growth in cells. It also contributes to human evolution.
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| | Z703915 | Lehninger Principles of Biochemistry, 4th ed. | The Fourth edition presents the fundamentals of biochemistry through selected topics, incorporating the most important recent developments and applications into its singular presentation of the field's classic core. For the new edition, authors David Nelson and Michael Cox offer groundbreaking new treatment of metabolic regulation, up-to-date coverage of DNA-based information technologies, and many new medically relevant applications. Also new is an innovative graphical style for depicting enzyme reaction mechanisms, step by step. While incorporating this essential information, the new edition has been streamlined to be 10% shorter.
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 | Z700959 | Liposomes 2nd ed., A Practical Approach | This edition contains significantly updated chapters from the 1st edition and several new chapters. The first half of the book addresses a number of essential experimental protocols such as the preparation, characterization, and storage of liposomes. Also included are methods for drug encapsulation into liposomes, modification of the liposomal surface to control drug behavior in biological environments, long-circulating liposomes and the use of cationic liposomes as transfection vectors. The second half discusses specific areas of liposome research and application, such as pH-sensitive liposomes, liposomes for diagnostic imaging, liposomal DNA vaccines, isothermic titration calorimetry of liposomes and vesicular phospholipids gels.
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| | Z378143 | Liposomes in Gene Delivery | Pharmacologists are familiar with liposomal drug delivery systems, and molecular biologists are comfortable with the concepts of genetic manipulation with DNA vectors. Gene delivery with liposomes is where the twain meet, and this volume aims to clarify the interface. An introductory text for nonviral gene therapy, it uses clear explanations and illustrations to prepare readers to perform well designed experiments with appropriate controls.
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 | Z702188 | Macrolide Antibiotics: Chemistry, Biology and Practice, 2nd ed. | This book explores the discovery of new macrolide antibiotics, their function, and their clinical use in diseases such as cancer, AIDS, cystic fibrosis and pneumonia. It discusses the creation of synthetic macrolides and the mechanisms of antibiotic activity. The uses for antimicrobial macrolides in clinical practice are also covered. This book is designed to appeal to both the basic and applied research communities interested in microbiology, bacteriology, and antibiotic/antifungal research and treatment
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| | Z718289 | Manipulating the Early Embryo of Xenopus laevis: A Video Guide | The large, readily available eggs of enopus laevis are a valuable subject for study. This 3-volume video set contains 68 demonstrations of the manipulation of living eggs using techniques taught in the course on the Early Development of Xenopus laevis held at Cold Spring Harbor Laboratory, New York, for the past four years. The video was produced using digital techniques to ensure no loss of resolution of the fine images captured by microphotography. Recorded live among the course participants, the videotape accurately reflects the triumphs and tribulations of real investigators as they attempt painstaking procedures while commenting on their actions for the students’ benefit. This video guide is an essential learning tool for investigators considering work with Xenopus eggs and embryos.
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 | Z718556 | Manipulating the Mouse Embryo: A Laboratory Manual (Third Edition) | Regarded as the "Bible" of mammalian embryo manipulation techniques since the 1986 publication of the first edition of Manipulating the Mouse Embryo: A Laboratory Manual, the third edition of this essential laboratory resource has now been completely reorganized, rewritten, and updated by a new cast of authors. The result is a compilation of new, cutting–edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques. The "gold standard" techniques for applying recombinant DNA technology to investigations of mammalian embryonic development included in the first two editions of this book are also updated and recast, as is the summary of the current state of understanding of mouse development at the molecular level. This book is the premier authoritative and comprehensive source of technical and theoretical guidance for mouse developmental biologists and geneticists and is an essential resource for newcomers to these fields.
Key features of the third edition:
New authors are leading authorities in mouse development and genetics, as were authors of prior editions.
All new illustration and new design: All illustrations have been redrawn; extensive use of new illustrations and photographs of techniques and results; a new, contemporary design presents techniques more clearly.
New explanatory text: More background information for techniques is presented as well as more extensive troubleshooting information.
New, functional organization and techniques.
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 | Z700401 | Manipulating the Mouse Embryo: A Laboratory Manual, 3rd ed. | Regarded as the "Bible" of mammalian embryo manipulation techniques, the third edition has been completely reorganized and rewritten. The result is a compilation of new, cutting-edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques.
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 | M1563 | Matrix Metalloproteinase Protocols | Experts detail proven laboratory techniques for the study of MMPs. Methods include those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a variety of circumstances. Each method includes step-by-step instructions and notes variant applications and pitfalls to avoid. A selective overview of the MMP arena states where the field has been, where it is, and where it is going.
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 | M7184 | Matrix Metalloproteinases and TIMPs | This book contains comprehensive data about matrix metalloproteinases (MMPs), enzymes which break down the extracellular matrix. It covers their sequences, three-dimensional structure, activation, substrates, specificity, inhibition, biological roles and function, along with protein sequence alignments. MMPs are regulated by their inhibitors (TIMPs). This volume contains sequences, three-dimensional structures and biological roles of TIMPs along with over 1700 references on MMPs and TIMPs.
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 | Z730297 | Measuring Gene Expression | In the post-genomic age, much biomedical research looks at when, where, and at what level genes are expressed. Measuring Gene Expression is an all-in-one introduction to the main methods of measuring gene expression, including RT-PCR, differential display, RNA interference, reporter genes, microarrays, and proteomics, as well as a section on RNA isolation and analysis. There is an overview of each method: its pros and cons, sample preparation, sources of error, and data interpretation.
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 | Z306290 | Medical Virology: AÂ Practical Approach | Like other biomedical sciences, medical virology has undergone a revolution of diagnostic and scientific approaches through the advent of molecular biological techniques. Developing and maintaining an appropriate mixture of classical and molecular techniques for viral analysis is one of the challenges of medical virology today, and this volume addresses these issues. Topics covered include a broad description of "classical" techniques in viral diagnosis, nucleic acid detection by extraction and hybridization, use of the polymerase chain reaction, the application of various molecular techniques to aspects of the epidemiology of virus infections, and the principles and practical approaches to the analysis of viral evolution.
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| | Z364053 | Membrane Protein Expression Systems: AÂ User’s Guide | This book is a simple-to-read laboratory manual describing the principles behind the commonly used expression systems, e.g. Escherichia coli, cell culture systems, Xenopus oocytes, baculovirus, vaccinia and yeast. Detailed experimental protocols for each system are provided in an easy-to-use, step-by-step format. The benefits of each system, as well as the likely pitfalls that newcomers might encounter, are discussed in each case.
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 | M4684 | Membrane Transport: A Practical Approach | This book will be of interest to those working on the mechanism of solute transport across biological membranes. Its scope is broad, ranging from the techniques required to study transport itself, through to the expression, purification and reconstitution of transporters, and techniques for investigation of their structure. It includes computer prediction of transporter topology and structure. Most of the protocols can be implemented in any well-found laboratory.
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 | Z730270 | Metabolomics: Methods and Protocols | This book contains protocols, which provide the essential groundwork for efforts in elucidating the structure of the unknowns detected in metabolomic studies. Part I focuses on GC-MS techniques; specifically to metabolite profiling in blood plasma. In Part II, emphasis shifts to data integration and data mining techniques, and specific protocols discuss improving pattern recognition and biomarker selection for systems level approaches. Parts III, IV, and V examine the coupling of capillary electrophoresis and mass spectrometry, classical reversed phase liquid chromatography-mass spectrometry, and electrochemical detection of metabolites using HPLC separations coupled with coulometric electrode array detectors. The volume concludes with an examination of cutting-edge NMR protocols.
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 | MIE272 | Methods in Enzymology Series | A selection of titles from the long-running series published by Academic Press.
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 | Z703869 | Microbial Enzymes and Biotransformations | Experts in enzyme manipulation describe in detail their techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Overview chapters on microorganisms as a source of metabolic and enzymatic diversity, and on the fast-moving field of enzyme biosensors are presented. This book offers proven enzymatic protocols that show clearly how to go from laboratory results to industrial applications.
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 | M0183 | Molecular Biomethods Handbook | Investigators illuminate the core bioanalytical techniques used in their own laboratories. These highly experienced scientists fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few.
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 | Z718181 | Mutants of Maize | This unique work of reference provides quick and easy access to the remarkable diversity of mutant phenotypes expressed by the maize plant. Its major portion consists of over 400 color photographs of well-established mutants arranged by chromosome position, detailed descriptions of the mutants' gene loci, and a current summary of the physical structure of genes that have been characterized at the molecular level. First published in 1968 and long out of print, Mutants of Maize has been entirely revised by three of the corn plant's most distinguished investigators, enlarged to cover twice the number of mutants, and redesigned with clarity and elegance. All maize biologists will want this magnificent book in their personal library. In addition, because mutant maize genes have counterparts in most species, the book can be considered a general guide to the consequences of mutation in the entire plant kingdom. All maize biologists will want this magnificent book in their personal library. In addition, because mutant maize genes have counterparts in most species, the book can be considered a general guide to the consequences of mutation in the entire plant kingdom.
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 | Z718203 | Mutants of Maize | This unique work of reference provides quick and easy access to the remarkable diversity of mutant phenotypes expressed by the maize plant. Its major portion consists of over 400 color photographs of well-established mutants arranged by chromosome position, detailed descriptions of the mutants' gene loci, and a current summary of the physical structure of genes that have been characterized at the molecular level. First published in 1968 and long out of print, Mutants of Maize has been entirely revised by three of the corn plant's most distinguished investigators, enlarged to cover twice the number of mutants, and redesigned with clarity and elegance. All maize biologists will want this magnificent book in their personal library. In addition, because mutant maize genes have counterparts in most species, the book can be considered a general guide to the consequences of mutation in the entire plant kingdom. All maize biologists will want this magnificent book in their personal library. In addition, because mutant maize genes have counterparts in most species, the book can be considered a general guide to the consequences of mutation in the entire plant kingdom.
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| | Z369314 | New Methods in Peptide Mapping for the Characterization of Proteins | New procedures for characterizing recombinant DNA-derived proteins by peptide mapping are presented. Among these are capillary electrophoresis, analysis of glycopeptides, and the use of electrospray and matrix-assisted laser desorption mass spectrometry. Practical procedures for sample preparation, enzyme digestion, separation, and structural analysis of the separated species are also provided.
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 | Z704229 | Nitric Oxide Donors: For Pharmaceutical and Biological Applications | Nitric oxide is a highly potent regulatory molecule with great pharmaceutical potential. This handbook fills a real gap in combining the chemistry of nitric oxide releasing substances with their practical applications in biology and drug design. It covers all classes of nitric oxide donors, from organic nitrates to nitroso compounds, guanidines and metal-NO complexes. In addition to a detailed treatment of the chemistry of NO donors, numerous examples of successful diagnostic and pharmacological applications are discussed, as well as further therapeutic targets for these substances.
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 | N7654 | Nuclease Methods and Protocols | Leading scientists offer their methodologies for studying protein nucleases to determine their activity, structure, interaction with other molecules, and physiological role. Protocols range from methods for characterizing nucleases, to correlating nuclease structure and function, to the use of nucleases in cloning. Recombinant techniques for expressing and isolating nucleases are covered, as well as methods specific for dealing with protein/nucleic acid complexes. Each chapter contains a review of a specific nuclease or nuclease-related theme, a discussion of why and when to use the assays described, and an explanation how the assay was developed.
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| | Z357472 | Nucleases, 2nd ed. | This new edition provides a comprehensive review of all classes of nuclease, their modes of action and biological significance. It is an invaluable source of information for investigators of DNA replication, recombination and repair and RNA processing.
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 | O1389 | Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols | This book presents cutting-edge molecular and ultrastructure techniques for investigating the pathophysiology of oxidative stress. Described in step-by-step detail, these readily reproducible methods use the latest in immunodetection, x-ray diffraction, confocal microscopy, in situ hybridization, chemiluminescence, and cytofluorescence. Additional techniques take advantage of electron paramagnetic resonance, advanced glycation endproducts, Western blotting analysis, transcription factors, p47 phox translocation, and microsphere delivery systems.
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 | O1264 | Oxidative Stress Biomarkers and Antioxidant Protocols | This is a collection of methods for evaluating perturbations in cell function resulting from increased oxidative stress. These techniques cover free radical-derived and antioxidant biomarkers. Methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, GC-MS, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation.
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 | P1994 | Peptide Antibiotics: Discovery, Modes of Action and Applications | This reference summarizes the latest research on the structure, function, and design of synthetic and natural peptide antibiotics, describing practical applications in food preservation and packaging, and in the prevention and treatment of infectious diseases by direct antibacterial action and as part of the adaptive immune response. It discusses these compounds and their applications including: the distribution and classification of diverse, antimicrobial peptides throughout nature, the role in host defense of mucosal surface peptide antibiotics such as defensins and cathepsins, and the genetic basis determining the production of bacterial peptide antibiotics.
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 | Z703826 | Peptide Synthesis and Applications | Experts describe in detail the methodologies of contemporary peptide synthesis and illustrate their numerous applications. Techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.
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 | Z553751 | Peptides: Biology and Chemistry | Peptides play a decisive role in many physiological processes, whether as neurotransmitters, hormones or antibiotics. The rapid developments in peptide research over the past few decades make it almost impossible for newcomers to gain an overview. This unique work covers all the important aspects of this wide-ranging field in one handy volume. On the basis of the fundamental chemical and structural properties of peptides, this reference runs the gamut from analysis, the occurrence and biological importance of peptides, via chemical, biochemical and genetic methods of peptide synthesis, right up to peptide libraries, peptide design and their role in drug research. Yet this book offers much more than a mere overview of the latest level of research. An encyclopedic appendix with valuable data on more than 500 biological relevant peptides and proteins, a comprehensive register and details of further literature references make this the ideal reference for all questions regarding peptide research.
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 | Z704423 | Phosphodiesterase Methods and Protocols | Research leaders describe new concepts and techniques for investigating the role of PDEs in orchestrating normal and pathophysiological responses. These methods allow the measurement of cyclic nucleotide variations in living cells, as well as their visualization in a spatio-temporal manner, the localization and characterization of their activities in tissues and living cells, and the assessment of targeted PDEs in creating specific tools and drugs. Additional chapters discuss the generation of PDE4 knockout mice to demonstrate not only the potential role of targeted PDEs, but also their use in further studies of the central role of PDE regulation in intracellular signaling control. Alterations of intracellular signaling related to PDE deregulation in such pathologies as inflammation, neurodegeneration, and cancer may explain the difficulties observed in their prevention and treatment.
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 | P1603 | Phospholipid Signaling Protocols | This collection of state-of-the-art techniques for the assay of phospholipid and phospholid-derived second messengers allows the identification and quantitation of signal pathway activation. Techniques cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase activities and for the separation and identification of phospholipids, diacylglycerol and sphingolipids, as well as their phosphoinositol, choline and fatty acid metabolites.
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 | Z701815 | Plant Protease Inhibitors Significance in Nutrition, Plant Protection, Cancer Prevention and Genetic Engineering | Plant protease inhibitors are diverse in number and in specificity towards various proteolytic enzymes. This book focuses on the isolation, structure, characterization, physiochemical and physiological properties, synthesis, functions and mechanisms of action of plant protease inhibitors. It deals with their inhibitory effects on insects and pathogens and with their induction and introduction into plants by genetic engineering for plant protection. The induced resistance of insects to the inhibitors and the hazards to friendly insects are also discussed. Findings are presented on the cancer chemopreventive properties of the inhibitors at the cellular, tissue and organ levels and in the organism
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 | Z378402 | Practical Approach Series on CD-ROM | This CD-ROM contains 68 Practical Approach titles in encrypted form. Purchase entitles you to unlock any 10 titles of your choice. Titles can be browsed and searched with a sophisticated and attractive user interface. Search chapter, volume, or all volumes with powerful Boolean logic. Personalization features allow you to set annotated bookmarks and attach notes to sections of a book. Any portion of the series can be copied to the Clipboard or printed out. You can even search titles that are still locked, and that way determine which additional titles you might want to unlock. By contacting the publisher directly, you can purchase access to additional titles.
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 | Z702781 | Practical Enzymology | Providing a broad experimental approach, this book contains a theoretical introduction together with practical protocols, considering all aspects of enzymology. The fundamental experiments in enzymology are presented in very clear and easily realizable protocols. Each experiment is accompanied by a section giving the theoretical background. This practical guide is an ideal supplement to "Enzyme Kinetics: Principles and Methods" (E2529). Essential reading for all scientists and practitioners working with enzymes.
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 | P7242 | Principles of Development, 2nd ed. | The authors’ approach is the idea that understanding how genes control cell behavior is the key to understanding development. Resisting the temptation to cover every aspect of developmental biology, the authors have instead focused on those systems that best illuminate common principles. The theme is that universal principles govern the process of development. The text also focuses on vertebrates and Drosophila, but without excluding other systems, such as the nematode and the sea urchin. Another feature is the inclusion of the development of plants--usually neglected in other textbooks.
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 | Z701912 | Prion Biology and Diseases,2nd ed. | This volume is a new edition of the most authoritative book on Prion Biology, first published in 1999 and edited by the Nobel Prize–winning founder of the field. This expanded edition has been completely updated, and includes chapters on therapeutics, and diagnostic methods and approaches This book is by far the most authoritative summary of the complex data that form the basis of our understanding of the molecular basis of this esoteric group of diseases.
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 | Z701009 | Protein Kinase C Protocols | The first compilation of classic and emerging laboratory techniques for the study of the biochemistry and cell biology of protein kinase C (PKC). These detailed methods can be used to explore the structure, function, regulation, subcellular localization, and macromolecular interactions of protein kinase C.. Each protocol is introduced in the context of PKC function and regulation and contains many notes on how best to deal with the problems that may occur. It is a compilation of biophysical, biochemical, cell biological, and molecular biological approaches that brings protein kinase C research into any laboratory.
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 | P3113 | Protein Kinase Functions | This provides a new focus on context and function of protein kinases, thus reflecting recent advances in kinase biology. Chapters on genetic approaches to protein kinase functions, MAP kinase pathway, and cyclin-dependent kinases are updated. Topics covered in depth are phosphatidylinositol 3-kinase signaling, JAK-STAT signaling, suppression of tyrosine kinases by the SOCS family proteins, TGFss superfamily, and involvement of protein kinases in response to DNA damage. Throughout, emphasis is placed not on individual kinases, but on functional aspects of the whole system and relationship between processes and molecules.
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 | P5611 | Protein Kinase Protocols | Basic and more sophisticated methods are described for the analysis of kinase-mediated signaling cascades, with emphasis on the identification of proteins according to their interactive relationships and the analysis of their functional properties. Emphasis is given to critical technical steps that are often omitted from other published methods. This offers tools for understanding the functional roles of specific protein kinases within signaling cascades and for identification and evaluation of novel therapeutic targets.
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 | Z704725 | Protein Tyrosine Kinases: From Inhibition to Useful Drugs | Leading researchers survey the state of the art in the drug discovery processes aimed at generating PTK inhibitors for the treatment of various diseases, including cancer. Highlights include a discussion of the rationale and the progress made towards generating “selective” low molecular-weight kinase inhibitors; an analysis of the normal function, role in disease, and application of platelet-derived growth factor antagonists, and a summary of the factors involved in successful structure-based drug design. Additional chapters address the advantages and disadvantages of in vivo preclinical models for testing protein kinase inhibitors with antitumor activity and the utility of different methods in the drug discovery and development process for determining “on-target” vs “off-target” effects of kinase inhibitors.
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 | Z702307 | Proteinase and Peptidase Inhibition | Cellular proteinases and their physiological role in normal and disease states have been the subject of great interest recently. Proteinase and Peptidase Inhibition presents new reports on a wide range of cellular proteinases whose inhibitors may hold the key to the development of new drugs. It deals with enzyme classes, inhibitor types and regulatory mechanisms governing proteinase activity. Target enzymes are fully described and classified by structural similarity, rather than by pharmacological effects. This is a valuable reference for researchers in medicinal chemistry, drug design and development, pharmacology, biochemistry, and molecular and cell biology.
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 | P0868 | Proteins, Peptides and Amino Acids SourceBook | Building on the success of their Source Book of Enzymes, the authors have assembled a catalog of over 26,000 commercially available proteins, peptides, and amino acids. All are arranged alphabetically and by sequence for fast access, and are replete with technical details and vendor information. Compounds can be easily located by either directly searching the appropriate section by chemical name or by consulting the general index by name, synonym, or derivative formula. The data covers sequence, sequence modification, chemical derivatives, preparation form, purity, composition, activity, functionality, as well as applications and literature references.
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 | P7492 | Proteins: Biochemistry and Biotechnology | This book provides an overview of the various medical, diagnostic and industrial uses of proteins. It has evolved from an earlier text “Protein Biotechnology” and includes the latest developments in the field and offers a varied selection of relevant case study material. Initial chapters focus upon issues such as protein structure, folding, stability, purification and characterization. Later chapters detail the industrial production of proteins, and their applications in medicine, analysis and industry. It presents a comprehensive review of proteins and enzymes used in the diagnostic, therapeutic and industrial areas.
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 | P2863 | Proteolytic Enzymes: A Practical Approach, 2nd ed. | This second edition has been thoroughly updated to take into account advances in the last 10 years in both the increased understanding of the role of peptidases in many critical cellular processes, e.g. apoptosis, as well as their use as enzymatic tools in such diverse areas as proteomics, protein sequencing, peptide synthesis and protein structure studies. Some topics covered are: inhibition and prevention of unwanted proteolytic activity; characterizing natural inhibitors; proteolytic enzymes in peptide mapping and primary structure elucidation by mass spectrometry and Edman sequencing; limited proteolysis as a structural probe.
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 | Z702110 | Recognition of Carbohydrates in Biological Systems, Part A: General Procedures, MIE Vol. 362 | Recognition of carbohydrates in biological systems has been gaining more and more attention in recent years. Although methodology for studying recognition has been developing, there is no volume that covers the wide area of methodology of carbohydrate recognition. This volume and its companion, Volume 363, present state-of-the-art methodologies, as well as the most recent biological observations in this area. It covers the isolation/synthesis of substances used in studying interactions involving carbohydrates and discusses the methodology for measuring such interactions. Biological roles for such interactions are also covered.
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 | Z702129 | Recognition of Carbohydrates in Biological Systems, Part B: Specific Applications, MIE Vol, 363 | Recognition of carbohydrates in biological systems has been gaining more and more attention in recent years. Although methodology for studying recognition has been developing, there is no volume that covers the wide area of methodology of carbohydrate recognition. This volume and its companion, Volume 362, present state-of-the-art methodologies, as well as the most recent biological observations in this area. It covers carbohydrate-binding proteins and discusses glycoproteins and glycolipids. Polysaccharides, enzymes and cells are also covered
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 | Z702854 | Recombinant Gene Expression:Reviews and Protocols, 2nd ed. | In this edition, the editors have added critical reviews to a collection of protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of non-protein molecules. The protocols offer new host-vector systems, such as Anarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding.
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 | Z718521 | Retroviruses | For over 25 years the study of retroviruses has underpinned much of what is known about information transfer in cells and the genetic and biochemical mechanisms that underlie cell growth and cancer induction. Emergent diseases such as AIDS and adult T-cell lymphoma have widened even further the community of investigators directly concerned with retroviruses, a development that has highlighted the need for an integrated understanding of their biology and their unique association with host genomes. This remarkable volume satisfies that need. Written by a group of the field's most distinguished investigators, rigorously edited to provide a seamless narrative, and elegantly designed for clarity and readability, this book is an instant classic that demands attention from scientists and physicians studying retroviruses and the disorders in which they play a role.
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 | Z718858 | Retroviruses CD | For over 25 years the study of retroviruses has underpinned much of what is known about information transfer in cells and the genetic and biochemical mechanisms that underlie cell growth and cancer induction. Emergent diseases such as AIDS and adult T-cell lymphoma have widened even further the community of investigators directly concerned with retroviruses, a development that has highlighted the need for an integrated understanding of their biology and their unique association with host genomes. This remarkable volume satisfies that need. Written by a group of the field's most distinguished investigators, rigorously edited to provide a seamless narrative, and elegantly designed for clarity and readability, this book is an instant classic that demands attention from scientists and physicians studying retroviruses and the disorders in which they play a role.
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 | Z375667 | Ribozyme Protocols | A comprehensive collection of essential and easily reproducible techniques currently used by leading researchers to manipulate RNA and to inactivate gene expression in vivo. An indispensable volume not only for workers new to the field, but also for experienced researchers who want to expand their area of expertise. Some of the topics covered:
• Synthesis of ribozymes in vitro and their application in vivo
• Ribozyme design rules and production by transcription
• Clinical potential of ribozymes
• Computer programs that predict target Rna folding and help select ribozyme cleavage sites
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 | S2564 | SV40 Protocols | Volume 165 of the Methods in Molecular Biology Series describes key techniques for experimentally detecting SV40 in human tumors, for exploiting its use in human gene therapy, and for studying its replication and its mechanisms of neoplastic transformation. Included are methods for growing SV40 and related viruses in tissue culture, for in vivo and in vitro replication and transcription of SV40 DNA, for use of retroviral vectors to express SV40 tumor antigens in cultured cells, and for use of transgenic mouse models based on the SV40 large T antigen.
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| | Z369225 | Stealth Liposomes | This book examines stealth liposomes from a multidisciplinary approach, which includes theoretical polymer physics, organic synthesis, colloid science and biology. Discussions include theory, chemistry, biochemistry, pharmacology, preclinical studies in model systems, and medical applications in humans.
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 | Z700533 | Structural Bioinformatics | This text provides an understanding of the theories, algorithms, resources, and tools used in structural bioinformatics. Reader emerges with the ability to make effective use of protein, DNA, RNA, carbohydrate, and complex structures to better understand biological function. It draws a clear connection between structural studies and the rational design of new therapies. This text covers the theory, biology, resources, and practical applications for computational approaches to biomolecular structural studies and features editors who have made significant contributions to the Protein Data Bank, the central repository for structural information.
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 | Z718432 | Structure and Reproduction of Corn | The 1949 publication The Structure and Reproduction of Corn, by Theodore Kiesselbach is an indispensable source of knowledge for plant biologists throughout the world but has long been unavailable in printed form. To mark the fiftieth anniversary of the appearance of this important work, a new edition has been published on high quality paper that brings out the best in the text’s finely detailed illustrations. This edition includes a historical introduction that illuminates the man behind the science, an investigator who applied the lessons of studies of hybrid vigor to create a revolution in corn breeding that transformed the economy of the American prairie states. This small, attractive book is a must for everyone interested in plant development.
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 | S0317 | Superoxide Dismutase: Methods in Enzymology Volume 349 | Many of the new and updated methods and techniques necessary for the investigation of SOD and the superoxide radical are described in this volume. Topics covered include superoxide reactions and mechanisms; mutants, knockouts, and transgenics; superoxide dismutase mimics; in vivo sources and cell signaling; and superoxide dismutase in aging and disease therapy. It serves as a standard to understanding the role of superoxide and SOD in basic biological systems and biological science.
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 | Z704350 | Systems Biology in Practice | Presenting the main concepts, this book leads reader from different disciplines to an understanding of current ideas in the complex field of comprehensive experimental investigation of biological objects, analysis of data, development of models, simulation, and hypothesis generation. It provides readers with guidance on how a specific complex biological question may be tackled: How to formulate questions that can be answered; Which experiments to perform; Where to find information in databases and on the Internet; What kinds of models are appropriate; How to use simulation tools; What can be learned from the comparison of experimental data and modeling results; How to make testable predictions.
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 | Z369470 | Telomeres | A comprehensive account of telomere structure and function in a variety of organisms including yeast, Drosophila, ciliates, plants and mammals is presented. Topics covered include: an historical overview, telomere dynamics, localization, transcriptional silencing and the significance of length regulation.
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 | Z704512 | Textbook of Biochemistry with Clinical Correlations, 6th ed. | This textbook presents the biochemistry of mammalian cells, relates events at a cellular level to the subsequent physiological processes in the whole animal, and cites examples of human diseases derived from aberrant biochemical processes. The organization and content are tied together to provide the complete picture of biochemistry and how it relates to humans. Loaded with new material and chapters and brimming with detailed, full-color illustrations that clearly explain associated concepts, this sixth edition is an indispensable tool for students and professionals in the medical or health sciences.
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 | Z364223 | The Telomere | Telomeres, specialized structures at the ends of chromosomes, are involved in genetic phenomena with a potential role in the aging and malignant transformation of cells. This book introduces the central questions concerning telomeres, examines experimental data, and provides a large number of recent references.
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 | Z718696 | Translational Control of Gene Expression | Since the 1996 publication of Translational Control, there has been fresh interest in protein synthesis and recognition of the key role of translational control mechanisms in regulating gene expression. This new monograph updates and expands the scope of the earlier book but it also takes a fresh look at the field. In a new format, the first eight chapters provide broad overviews, while each of the additional twenty-eight has a focus on a research topic of more specific interest. The result is a thoroughly up-to-date account of initiation, elongation, and termination of translation, control mechanisms in development in response to extracellular stimuli, and the effects on the translational machinery of virus infection and disease.
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 | T0191 | Tyrosine Phosphoprotein Phosphatases, Second Edition | This edition provides a practical and accessible guide to this major family of proteins with particularly important insights into their molecular biology, biochemistry, and role in cellular physiology. Topics include the sequence, structure, and biochemical properties of PTPases, regulation of PTPase expression, substrate preferences and catalytic mechanisms, physiology, covalent modification, modification by chemical agents and proteolysis, and interactions with other proteins, peptides, and macromolecules. Featuring comprehensive coverage of the subject, including an extensive bibliography, this volume will provide an important resource for researchers and graduate students in molecular biology and biochemistry
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 | Z340898 | Virus Culture: A Practical Approach | This book provides a broad treatment of the principles and practice of virus culture. The first section is an overview of cell culture techniques essential for virologists. Other techniques covered are isolating, identifying, concentrating, and purifying viruses. Electron microscopy as applied to virology is explained. Topics include creating virus vaccines, antiserum production, monoclonal antibodies, antiviral drug testing and methods to study plant viruses.
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 | Z730521 | What’s Wrong With My Mouse Behavioral Phenotyping of Transgenic and Knockout Mice, 2nd ed. | Turn to What’s Wrong with My Mouse? to discover the wealth of mouse behavioral tasks and to get the guidance you need to select the best methods and necessary controls. Chapters are organized by behavioral domain, including measurements of general health, motor functions, sensory abilities, learning and memory, feeding and drinking, reproductive, social, emotional, and reward behaviors in mutant mice. Throughout the chapters, new behavioral tasks and new research discoveries have been added, bringing the Second Edition up to date with the latest science. In addition, it includes two new chapters: "Neurodevelopment and Neurodegeneration" discusses mouse behavioral tasks relevant to neurodevelopmental diseases, such as mental retardation and autism, and to neurodegenerative diseases, such as Alzheimers, Parkinsons, Huntingtons, and amyotrophic lateral sclerosis. "Putting It All Together" recommends strategies for optimizing a battery of behavioral phenotyping tests to address your specific hypotheses about gene functions.
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 | W2390 | What’s Wrong with My Mouse Behavioral Phenotyping of Transgenic and Knockout Mice | This concise review explains the differences between transgenic and knockout mice and their wild-type controls, while providing critical information about gene function and expression. Newly identified genes can provide insights into brain malfunctioning in disease states. It discusses how to generate a transgenic or knockout mouse, functions (open field, holeboard, rotarod, balance, grip, circadian activity, etc.), sensory abilities (olfaction, vision, hearing, taste, touch, nociception), reproductive behavior, social behavior, and emotional behavior.
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 | Z704776 | Yeast Protocols, 2nd ed. | This edition is a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components,including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.
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 | Z700398 | Zebrafish: A Practical Approach | The zebrafish has become one of the most important model organisms to study biological processes within a living body. As a vertebrate that has many of the strengths of invertebrate model systems, it offers numerous advantages to researchers interested in many aspects of embryonic development, physiology and disease. This book provides a complete set of instructions that will allow researchers to establish the zebrafish in their laboratory, and also gives a broad overview of commonly used methods and a collection of protocols describing the most powerful techniques.
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 | Z701262 | p53 Protocols | This collection of novel techniques has proven useful for studying the physiological properties of p53 both in vitro and in vivo. The techniques provide solutions to problems in studying the purification, target identification, gene expression, quantitation, interaction, signaling, transactivation, and transrepression of p53. The methods are also useful for delineating the functions of other proteins that may act as tumor or growth suppressors. Each technique includes step-by-step instructions, troubleshooting notes, a theoretical review, and discussion of associated problems.
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