Second Edition is completely updated, and expanded to reflect new advances. It contains over 80 entries on all currently cloned and characterized cell adhesion molecules, including the families of integrins, cadherins, selectins, members of the immunoglobulin superfamily, and other relevant receptors. It provides information on amino acid sequence of the most commonly studied organisms; gene organization and chromosomal location; alternative nomenclature; biological function; tissue distribution; disease association; database accession numbers.
The Biology of Cancer synthesizes the findings of three decades of recent cancer research and proposes a conceptual framework from which to teach about these discoveries. It provides the necessary structure, organization, and content for a course on cancer biology for advanced undergraduates and beginning doctoral students.
The book is comprehensive and offers many pedagogical features to assist teaching and learning. The text provides current information on topics such as tumor stem cells and recently introduced chemotherapeutics. State-of-the-art techniques are discussed throughout. Modern biomedical research is explored, helping readers to hone their analytical abilities and to assimilate and think clearly about complex biological processes.
The use of binatorial chemistry and peptide libraries in drug screening and development is a new and rapidly expanding technology. This first handbook on the topic contains background information and step-by-step experimental procedures. It will be indispensable for anyone working in the field.
This is a collection of protocols for investigating DNA structure, topology, and DNA topoisomerase function. These methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. Protocols detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases.
This book expertly summarizes the current status of development and application of deoxynucleoside analogs. Organized into several sections, the first part covers general aspects of drug uptake and metabolism and explains how novel technology has enabled a rapid expansion of this field. The second part is concerned with a number of specific drugs including cytarabine, gemcitabine, troxacitabine, clofarabine and ara-G. The final section covers pharmacokinetics, prodrugs, and specific applications such as radiosensitization, gene therapy, and the use of deoxynucleoside analogs as tracers. Throughout the book, the focus is on novel aspects of deoxynucleoside analogs in the clinical context, as well as on unexpected targets of these compounds, such as their specific activity against cell cycle-dependent kinases or oncogenes.
Techniques for manipulation of developmental gene expression and function, analysis of gene expression, characterization of tissue morphogenesis and development, in vitro study of differentiation and development, and genetic analysis of developmental models of diseases.
Recent developments have produced a wealth of experimental data on sequences and three-dimensional structures of biological macromolecules. As a result, the employment of various computational methods of analysis to obtain useful information is now a major new discipline: bioinformatics. This book provides a vital reference for important terms, offering a convenient summary of the core knowledge. With concise and accurate definitions of over 600 words, phrases, and concepts, this volume offers thorough A-Z coverage, lists references and Internet links, incorporates cross-referencing throughout, and features a comprehensive index of topics.
This book provides a guide to evaluate enzyme kinetics, using common software packages to perform enzymatic analyses. The treatment of enzyme kinetics here is radically different. Marangoni stresses an understanding of how researchers arrive at models, what the models′ limitations are, and how they can be used in ways to analyze enzyme kinetic data. Computers make linear transformations of models unnecessary, and this book does away with them, advancing the use of nonlinear regression techniques. Marangoni develops new ways to carry out analyses of enzyme kinetic data, particularly in the study of pH effects on catalytic activity and multisubstrate enzymes.
The sugar chains of cells-known collectively as glycans-play a variety of impressive, critical, and often surprising roles in biological systems. Glycobiology is the study of the roles of glycans in the growth and development, function, and survival of an organism. Glyco-related processes, described in vivid detail in the text, have become increasingly significant in many areas of basic research as well as biomedicine and biotechnology. This new edition of Essentials of Glycobiology covers the general principles and describes the structure and biosynthesis, diversity, and function of glycans and their relevance to both normal physiologic processes and human disease. Several new chapters present significant advances that have occurred since the publication of the first edition. Three sections of note describe organismal diversity, advances in our understanding of disease states and related therapeutic applications, and the genomic view of glycobiology. "Glycomics," analogous to genomics and proteomics, is the systematic study of all glycan structures of a given cell type or organism and paves the way for a more thorough understanding of the functions of these ubiquitous molecules. The first edition of Essentials of Glycobiology represented also a notable experiment in publishing, as it became one of the first electronic textbooks. And, now, in recognition of its wide audience and the changing ways in which researchers and students learn and access information, the new edition of Essentials will be made available online simultaneously with the print edition. This novel experiment is the result of the collaborative efforts of the Cold Spring Harbor Laboratory Press, the National Center for Biotechnology Information, and the editors of the book. Written and edited by glycobiologists with experience in teaching and in research, this volume will be an invaluable resource, both for students and for established investigators in fields such as developmental biology, cell biology, neuroscience, immunology, and biochemistry who require a complete yet concise introduction to this burgeoning field.
More than 700 amino acids (AAs) have been found in nature and their number is continually increasing. Internationally renowned scientists critically examine the chemistry of fluorine-containing amino acids (FAAs) and the existing knowledge regarding their biological properties. A synthesis of FAAs by methods of acid, organofluorine and organometallic chemistry; enzymatic and chromatographic resolution of racemic FAAs; biological characteristics of FAAs and their applications are among the subjects covered.
This new volume covers essential procedures for production of linear peptides and more advanced techniques for preparing cyclic, side-chain modified, phospho- and glycopeptides. Many other methods have been included: convergent peptide synthesis; peptide-protein conjugation; chemoselective ligation; and chemoselective purification. The difficult preparation of cysteine and methionine-containing peptides is covered, as well as methods for overcoming aggregation during peptide chain assembly.
This concise introduction to peptide chemistry serves as a step-by-step guide, emphasizing the principles of peptide chemistry, with its strong links to physical and organic chemistry and biochemistry. Intended primarily for advanced undergraduates, it reflects the increasing importance of peptide chemistry, particularly in relation to solid-phase synthesis, gene sequencing and x-ray crystallography. Chapters cover amino acids, purification and isolation, amino acid analysis, sequencing and synthesis. A concluding chapter discusses the structure of LH-RH.
This book is a simple-to-read laboratory manual describing the principles behind the commonly used expression systems, e.g. Escherichia coli, cell culture systems, Xenopus oocytes, baculovirus, vaccinia and yeast. Detailed experimental protocols for each system are provided in an easy-to-use, step-by-step format. The benefits of each system, as well as the likely pitfalls that newcomers might encounter, are discussed in each case.
Recognition of carbohydrates in biological systems has been gaining more and more attention in recent years. Although methodology for studying recognition has been developing, there is no volume that covers the wide area of methodology of carbohydrate recognition. This volume and its companion, Volume 363, present state-of-the-art methodologies, as well as the most recent biological observations in this area. It covers the isolation/synthesis of substances used in studying interactions involving carbohydrates and discusses the methodology for measuring such interactions. Biological roles for such interactions are also covered.
Recognition of carbohydrates in biological systems has been gaining more and more attention in recent years. Although methodology for studying recognition has been developing, there is no volume that covers the wide area of methodology of carbohydrate recognition. This volume and its companion, Volume 362, present state-of-the-art methodologies, as well as the most recent biological observations in this area. It covers carbohydrate-binding proteins and discusses glycoproteins and glycolipids. Polysaccharides, enzymes and cells are also covered
Telomeres, specialized structures at the ends of chromosomes, are involved in genetic phenomena with a potential role in the aging and malignant transformation of cells. This book introduces the central questions concerning telomeres, examines experimental data, and provides a large number of recent references.
Turn to What’s Wrong with My Mouse? to discover the wealth of mouse behavioral tasks and to get the guidance you need to select the best methods and necessary controls. Chapters are organized by behavioral domain, including measurements of general health, motor functions, sensory abilities, learning and memory, feeding and drinking, reproductive, social, emotional, and reward behaviors in mutant mice. Throughout the chapters, new behavioral tasks and new research discoveries have been added, bringing the Second Edition up to date with the latest science.
In addition, it includes two new chapters: "Neurodevelopment and Neurodegeneration" discusses mouse behavioral tasks relevant to neurodevelopmental diseases, such as mental retardation and autism, and to neurodegenerative diseases, such as Alzheimers, Parkinsons, Huntingtons, and amyotrophic lateral sclerosis. "Putting It All Together" recommends strategies for optimizing a battery of behavioral phenotyping tests to address your specific hypotheses about gene functions.