 | P2481 | 2-D Proteome Analysis Protocols | Leading investigators in the field show how to make protein extracts, reproducibly run them on 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains.
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 | Z730394 | Advanced Technologies in Biopharmaceutical Processing | An increasing number of pharmaceuticals in human and veterinary medicine are being developed using advanced genetic and other methods that focus on modification of somatic and embryonic cells. These methods call for new processes that go beyond the traditional unit processes of chemical and biological production. This book is the first to describe these advanced biological processes and show how they are applied to the production of biopharmaceuticals, from product generation and purification to fill-finish operations. The work explains how these technologies function similarly to unit operations for producing advanced biopharmaceuticals, such as hormones, modified proteins, etc. From large-scale animal cell bioreactors to patient-customized products, this volume guides users on how to apply new technologies in process development.
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 | Z702226 | Affinity Capillary Electrophoresis in Pharmaceutics and Biopharmaceutics | This reference presents recent breakthroughs and techniques in affinity capillary electrophoresis (ACE) to measure and determine the physicochemical and thermodynamic parameters of drug compounds—offering strategies to explore and characterize interactions between drugs, drug vehicles, and biological membranes to facilitate developments in controlled drug delivery and targeting. This book contains current applications and procedures to identify binding constants between drugs, biological structures, proteins, nucleic acids, and plasmids; increase drug bioavailability; enhance the permeability, solubility, strength, and function of drug formulations; examine protein-protein exchanges and reactions; calculate pKa values.
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 | A5847 | Affinity and Immunoaffinity Purification Techniques | Application of affinity and immunoaffinity ligands is an essential aid in the selective isolation of specific analytes. Techniques have nuances, such as ligand orientation, ligand stability, and bound analyte recovery, that can hinder successful isolation of the desired material and result in suboptimal or nonrecovery. Other reasons for failure are that separation science approaches an art form, and that tricks essential for success are lost in the text or missing from published articles. This is a sound guide for those wishing to use these techniques in their experimental designs.
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 | A6341 | Automation Technologies for Genome Characterization | For end users of automated laboratory systems, this book will be a valuable reference for learning about appropriate technologies used in human genome characterization. It outlines how to access information about organizations and laboratories active in this field.
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 | Z719102 | Basic Methods in Microscopy | Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manuals Cells: A Laboratory Manual and Live Cell Imaging provide a solid course in the basics of using the microscope in a biology laboratory. This book provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events.
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| Z719099 | Basic Methods in Microscopy | Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manuals Cells: A Laboratory Manual and Live Cell Imaging provide a solid course in the basics of using the microscope in a biology laboratory. This book provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events.
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 | Z702625 | Bio conjugation Protocols: Strategies and Methods | Experts update the classic bioconjugation methods and introduce techniques that go beyond pure biomedical applications to include elements from organic synthesis, molecular biology, and materials science. These methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address on-chip synthesis to generate microarrays for the high-throughput analysis of RNA and DNA.
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 | Z704768 | Biocatalysts and Enzyme Technology | A comprehensive overview of current knowledge of biocatalysis and enzyme technology. Following an introduction to the history of enzyme applications and the motivations for using these highly selective and environmentally friendly methods, the book goes on to cover enzyme mechanisms and kinetics, production, recovery, characterization and their design, including recombinant methods. Alongside the application of soluble and immobilized biocatalysts, including whole-cell systems, the authors treat the use of non-aqueous reaction systems, applications in organic synthesis, bioreactor design and reaction engineering. In line with the book's didactic approach, a number of case studies further exemplify the advantages of enzyme processes. Each topic includes exercises, designed to facilitate access to this area of research.
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 | B1933 | Biochemical Methods:A Concise Guide for Students and Researchers | This book is the completely revised and updated English version of the very successful German language textbook, Methoden der Biochemie. It refers to laboratory equipment and safety aspects and explains how to obtain relevant biochemical information. It provides an introduction into physical-chemical processes and mathematical methods required for the interpretation of data. Principles of expensive instrumental analysis are also explained. In particular the presentation of safety considerations and regulatory issues has been adapted according to international requirements. It also contains a CD-ROM on quantitative analysis of biochemical experiments.
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 | Z374210 | Bioconjugate Techniques | A practical guide to modification and cross-linking technology for research, diagnostics and therapeutics. Contains detailed information on the chemistry, reagent systems and practical applications for creating labeled or conjugated molecules.
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| | Z269662 | Biodegradable Hydrogels for Drug Delivery | This book provides an in-depth investigation of recent advances in biodegradable hydrogels. Based on extensive research and experience, the book is the first to deal exclusively with this important area in drug delivery. A valuable guide for all, the book begins with a detailed introduction to current biodegradable hydrogel technology. Following chapters present advanced R&D, delivery systems, and more. It covers the mechanisms of biodegradation, types of biodegradable hydrogels, chemical and physical gels, chemical and enzymatic degradation, and examples of biodegradable drug delivery systems.
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 | Z379565 | Bioluminescence Methods and Protocols: Methods in Molecular Biology, Vol. 102 | A collection of methods for using luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, and cell-based assays. The contributors are those who have developed and perfected their methods. The wide variety of illustrative applications ranges from specific molecular titer to microbial detection and analysis. Other topics include the manipulation of the lux, luc, and gfp genes and construction of recombinant organisms. Cutting-edge techniques, detailed instructions, and diverse examples should make this book a standard reference.
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 | Z701106 | Biotechnology and Biopharmaceuticals: Transforming Proteins and Genes into Drugs | This book defines biotechnology from the perspective of pharmaceuticals. The first section focuses on the process of transforming a biologic macromolecule into a therapeutic agent, while the second section provides a brief overview of each class of macromolecule with respect to physiological role and clinical application. Additional detail is also provided in the second section for each FDA approved, recombinantly derived biopharmaceutical for each category of macromolecule. The final section looks to the future and the new advances that will enhance our ability to develop new macromolecules into effective biopharmaceuticals. This last section discusses various drug delivery strategies while also describing gene and cell therapy strategies.
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| | B5160 | Biotechnology from A to Z, 2nd ed. | Fully updated to incorporate the most currently terminology, this second edition offers an illuminating and practical introduction to the ideas of biotechnology. From ADEPT to zoonosis, this book provides a concise and readable overview of the field. It explains the usefulness of many techniques in this fascinating and controversial industry.
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 | Z373680 | Biotechnology: A Laboratory Course, 2nd ed. | this second edition consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry. New exercises on PCR and western blotting have been added, as well as new procedures on large-scale plasmid isolation and DNA quantitation. The procedures and principles demonstrated are widely applicable in the biological sciences.
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 | Z423319 | Biotransformations in Organic Chemistry: A Textbook, 4th Ed. | The use of biocatalysts, employed either as isolated enzymes or whole cells, offers a remarkable arsenal of highly selective transformations for modern preparative organic chemistry. During the last decade, this methodology has now generally been accepted as a complementary method to the already existing tools. After a basic introduction into the use of biocatalysts - principles of stereoselective transformations, kinetics, enzyme nomenclature and handling - the different types of reactions are explained according to the "reaction principle". The 4th edition includes new chapters on peroxidase reactions and on handling of biocatalysts.
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 | Z702838 | Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory | This book is the first comprehensive guide devoted exclusively to calculations encountered in the genetic engineering laboratory. This guide, written for students, technicians, and scientists, provides example calculations for the most frequently confronted problems encountered in gene discovery and analysis. The text and sample calculations are written in an easy-to-follow format. It is the perfect laboratory companion for anyone working in DNA manipulation and analysis.
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| | Z364347 | Capillary Electrophoresis | This is a complete, practical guide to the principles, forms, and instrumentation of capillary electrophoresis. The material on method development will be especially useful to those who are new to the technique.
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 | Z369411 | Capillary Electrophoresis Guidebook: Principles, Operation and Applications | Method development, quantitative procedures, optimization of precision and sensitivity, fraction collection and method validation are among the practical areas covered by this text. Specific techniques addressed include micellar electrokinetic capillary chromatography, capillary gel electrophoresis, advanced sampling techniques and electrochromatography.
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 | Z378135 | Capillary Electrophoresis in Analytical Biotechnology | The unique focus makes this methods book especially valuable to life scientists. Applications of CE to peptides for mapping proteins, to recombinant-expressed proteins for analysis of post-translational modification, to glycoproteins and oligosaccharides, and to nucleic acids round out the practical sections. A balanced account of the theory of CE helps practitioners develop an appreciation for methods development and for the capabilities and limitations of the technique.
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 | Z702641 | Capillary Electrophoresis of Proteins and Peptides | Expert academic and pharmaceutical researchers describe their best capillary electrophoresis techniques for protein and peptide analysis. The authors present cutting-edge techniques for both capillary coatings and analytic detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Areas of special interest covered include combining CE and capillary isoelectric focusing with electrospray mass spectrometry detection to perform proteomic studies and applications in the field of protein-ligand binding
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 | Z379832 | Capillary Electrophoresis: Theory and Practice, 2nd ed. | From the basic theory of separation and detection through real-world analytical problems, this up-to-date volume will appeal to students and everyday practitioners alike. Features new chapters on separation of inorganic ions and carbohydrates, as well as traditional biochemistry and biotechnology applications. Appendices provide lists of standard buffer solutions, instrument suppliers, and details of several student experiments.
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 | Z730661 | Centrifugal Separations in Biotechnology | This book is the first devoted to centrifugal separation in biotechnology. It is of value to professionals in the chemical, bioprocess, and biotech sectors, and all those concerned with bioseparation, bioprocessing, unit-operations and process engineering. Key topics covered include a full introduction to centrifugation, sedimentation and separation; detailed coverage of centrifuge types, including batch and semi-batch centrifuges, disk-stack and tubular decanter centrifuges; methods for increasing solids concentration; laboratory and pilot testing of centrifuges; selection and sizing centrifuges; scale-up of equipment, performance prediction and analysis of test results using numerical simulation.
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 | Z357421 | Centrifugation: Essential Data Series | This volume provides the core data required to carry out any centrifugal separation, whether working with cells, organelles, or macromolecules. Contents include types of centrifuges and rotors; gradient choice, preparation, fractionation and analysis; and other useful information.
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 | Z702609 | Chemical Genomics | The most comprehensive reference on this new and rapidly accelerating area of biotechnological innovation.This highly interdisciplinary reference/text identifies and elucidates the most influential developments in chemical genomics for improved research and advancement in a diverse array of fields ranging from proteomics, bioinformatics, and microfluidics to drug discovery and toxicology.
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 | Z702676 | Chemoinformatics: Concepts, Methods and Tools for Drug Discovery | The authors explain the theory behind the crucial concepts of molecular similarity and diversity, describe the challenging efforts to use chemoinformatics approaches to virtual and high-throughput screening, and illuminate the latest developments in multidimensional QSAR analysis. Other topics of interest include the use of partitioning algorithms and classification methods for analyzing large compound databases, screening sets, and virtual screening for active molecules; different approaches to target class-specific library design; and the generation of a novel class of molecular surface properties descriptors that can be readily calculated from 2D representations of molecular structures.
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| | Z364193 | Cryopreservation and Freeze-Drying Protocols: Methods in Molecular Biology, Vol. 38 | Each protocol addresses the preservation of a particular organelle, cell, or tissue type. Robust, reproducible survival is the goal of each technique; the reader's goal should be to attain the insights necessary to develop new protocols for novel situations.
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 | Z730688 | Crystallography Made Crystal Clear 3rd ed. | This book is the most comprehensive and concise reference for beginning Macromolecular crystallographers, written by an expert in the field. Rhodes' uses visual and geometric models to help readers understand the mathematics that form the basis of x-ray crystallography. He has invested a great deal of time and effort on World Wide Web tools for users of models. Rhodes' personal CMCC Home Page also provides access to tools and links to resources discussed in the text. Most significantly, the final chapter introduces the reader to macromolecular modeling on personal computers-featuring SwissPdbViewer, a free, powerful modeling program now available for PC, Power Macintosh, and Unix computers. This updated and expanded new edition uses attractive four-color art, web tool access for further study, and concise language to explain the basis of X-ray crystallography, increasingly vital in today's research labs.
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 | Z703222 | Crystallography Made Crystal Clear, 2nd ed. | This second edition explains how scientists discover the structures of the macromolecules. Scientists do not see these molecules directly. Instead, they build models as a means of interpreting data from x-ray diffraction by crystals, or by irradiation by other forms of energy. Users of these models need to know how they are obtained in order to know what they are seeing when they study a model of a macromolecule. They also need to know how to judge whether conclusions they draw from the molecular models are really supported by the models. This book uses visual and geometric models to help readers understand the mathematics that forms the basis of x-ray crystallography. The field of protein crystallography has been instrumental in discovering the molecular principles of biology and in discovering new drugs.
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 | C5231 | Cytoskeleton Methods and Protocols | Recent advances in experimental instrumentation, reagents and imaging technology have expanded the range of tools available for the study of the cytoskeleton. This book details reproducible methods for investigating cytoskeleton structure and function utilizing diverse model systems in a variety of organisms. Microscopy applications include digital-video microscopy and computer-assisted systems for the evaluation of cell motility and morphology. Help notes and tips accompany each protocol and provide additional information.
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| | Z511854 | Electrochemical Methods: Fundamentals and Applications, 2nd Edition | This edition is fully revised to reflect the current state off the field. Significant additions include ultramicroelectrodes, modified electrodes, and scanning probe methods. Many chapters have been modified and improved, including electrode kinetics, voltammetric methods, and mechanisms of coupled chemical reactions. Takes the reader from the most basic chemical and physical principles through fundamentals of thermodynamics, kinetics, and mass transfer, to a thorough treatment of all important experimental methods. Treats application of electrochemical methods to elucidation of reaction mechanisms; double layer structure and surface processes, and their effects on electrode processes are developed from first principles; other key features include a chapter on operational amplifier circuits and electrochemical instrumentation, unique coverage of spectrometric and photochemical experiments, and Laplace transform and digital simulation techniques.
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 | E8025 | Electron Microscopy Methods and Protocols | Experts describe key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological x-ray microanalysis, this book includes methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Immunogold labeling techniques for ultrastructural localization, distribution, and quantitation of macromolecules in cryofixed or chemically-fixed cells are described in detail to provide insight into their advantages and limitations.
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 | E8890 | Electron Microscopy: Principles and Techniques for Biologists. 2nd ed. | A direct, uncomplicated introduction to electron microscopy for biologists. This second edition has enhanced layout, illustrations, and text so that it is more accessible to the reader. Over 200 new line drawings and micrographs have been added.
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| | Z350125 | Electronic Light Microscopy: Techniques in Modern Biomedical Microscopy | This laboratory reference text offers guidelines for applying digital image processing methods to the analysis of electronic light microscope images of fixed and living biological specimens. It contains detailed discussions on: Image acquisition and sampling, image averaging and background “mottle” subtraction, and three dimensional image processing, animation, and display.
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 | Z703575 | Electrophoresis in Practice: A Guide to Methods and Applications of DNA and Protein Separations, 4th ed. | This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in use. This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references.
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 | E8778 | Enzyme Assays: A Practical Approach, 2nd ed. | This edition combines revised chapters with new contributions in describing the design and execution of enzyme assays. Topics include protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. Theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high-throughout screening; statistical analysis of enzyme kinetic data; and determination of active site concentration
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 | E7154 | Essentials of Genomics and Bioinformatics | Genomics is a new discipline that is revolutionizing the way biological and medical research is done. The human genome is almost completely sequenced, which will help to accelerate research and development in Genomics even more. The chapters in this book capture the rapidly evolving field. Overviews of nucleic acid technologies, proteomics and bioinformatics are followed by chapters on applications, including human health, agriculture and comparative genomics. The book also includes two chapters on legal and ethical issues of Genomics, a topic that is becoming more and more important.
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 | Z700940 | FISH, A Practical Approach | This laboratory manual covers the theory and application of the commonly used FISH procedures. Readers are provided with the theoretical principles which underlie each procedure, and are then guided through the protocols in a systematic manner. Because FISH procedures are particularly sensitive to background and subtle variations in sample quality, the trouble-shooting sections are invaluable to the beginner interested in developing FISH capabilities in their laboratory. Areas covered include probe production and labeling; FISH gene mapping; murine, fiber, and RNA FISH; CGH and microchips; 3D FISH; SKY and M-FISH; FISH and microarrays; and FISH in clinical cytogenetics.
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 | F0178 | Flow Cytometry: First Principles, 2nd ed. | With advances in flow cytometry, this edition provides an update, reflecting innovations while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. It explains the basic principles of flow cytometry, surveys scientific and clinical applications and highlights state-of-the-art techniques. This edition contains revisions of all chapters with new discussions on fluorochrome and laser options for multicolor analysis, an additional section on apoptosis in the chapter on DNA, and new chapters on intracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology.
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| | F6021 | Fluorescence Microscopy of Living Cells in Culture Part B | Fluorescence Techniques are uniquely suitable for probing living cells due to their sensitivity and specificity. Quantitative fluorescence microscopy − imaging and spectroscopy.
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 | F3426 | Fundamental Laboratory Approaches for Biochemistry and Biotechnology | Written by active scientists, the book provides readers with a logical framework for conducting modern biochemical and molecular biology research. It features 13 chapters on principles of biochemistry and molecular biology techniques, and 26 laboratory experiments covering basic methods. All reagents and supplies are listed with their Sigma Catalog numbers for ease of implementation. Unique chapter on using the Web for biochemistry research is provided along with a comprehensive Website that supports the book. (http://www.umich.edu/~aninfa/book/ )
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 | F9927 | Fundamentals of Light Microscopy and Electronic Imaging | Researchers need new skills in order to use a modern research microscope, such as how to align microscope optics and perform image processing. This book explores the basics of microscope design and use. It discusses optical principles involved in diffraction and image formation in the light microscope, basic modes of light microscopy, components of modern electronic imaging systems, and image processing operations necessary to acquire and prepare an image. It also reviews such topics as Illuminators, filters, and isolation of specific wavelengths; Phase contrast and differential interference contrast; Fluorescence and confocal laser scanning microscopy; Digital CCD microscopy and image processing.
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 | G4647 | Gel Electrophoresis of Proteins: A Practical Approach, 3rd ed. | | pricing |
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| | Z357413 | Gel Electrophoresis: Essential Data Series | This volume presents comprehensive data on gel electrophoresis of proteins, nucleic acids, nucleoproteins and carbohydrates. Also presented are sections on electrophoresis chemicals, types of apparatus, troubleshooting, and manufacturers and suppliers.
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 | G7541 | Genome Visualization by Classic Methods in Light Microscopy | In a two column format, the author investigates the classic techniques employed in the visualization of nucleic acids by light microscopy. The book discusses the chemical structure of nucleic acids, general principles of staining, nuclear dyes, and histochemical methods. Each chapter features different methods of staining, along with chemical principles and protocols. It also contains a color section with examples of observations.
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 | G2793 | Gold and Silver Staining: Techniques in Molecular Morphology | Immunogold silver staining is one of the most sensitive techniques for visualizing the location of antibodies and nucleotide probes. Contributors cover the latest developments and a range of applications from highly sensitive detection of antigens to single copy detection of DNA and RNA. This book presents descriptions that allow visualization of molecules never localized before. It also provides a selection of protocols with high sensitivity; presents techniques for cytochemistry, histochemistry, in situ hybridization, and immunoblotting applications; discusses the applications to cancer research; offers suggestions, tips, and guidelines to avoid unspecific precipitations; analyzes advantages and drawbacks of staining methods.
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 | G3793 | Green Fluorescent Protein: Applications and Protocols | This book demonstrates the power and the versatility of the green fluorescent protein (GFP) marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging); for using GFP to create and select for transgenic organisms, for its use as a biosensor; and for studying viruses. A valuable companion CD-ROM displays many color figures and videos from over half the chapters.
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 | Z378178 | Handbook of Capillary Electrophoresis, 2nd ed. | This fully updated edition pulls together diverse areas and applications of CE; the result is a volume that serves scientists in biotechnology, clinical, and pharmaceutical chemistry. The clear organization distinguishes general information − theory, modes of CE, detection, and data analysis − from specifics − with chapters on small molecules, peptides, proteins, carbohydrates, and nucleic acids as analytes. Some of the most useful chapters explore highly specialized applications of CE to large-scale DNA sequencing, drug analysis in biological fluids, binding studies, and enzyme assays.
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 | Z702277 | Handbook of Detection of Enzymes on Electrophoretic Gels, 2nd ed. | This edition makes significant additions to the set of reliable gene markers suitable for electrophoretic analysis. It also strengthens the value of enzyme electrophoresis as a tool proven successful in solving a variety of problems. New is an overview of applications of enzyme electrophoresis and zymogram techniques; zymogram techniques for approximately 100 enzymes not in the first edition, bringing the total to more than 900 methods for detecting more than 400 different enzymes; information on subunit structure included within the enzyme sheets to facilitate interpretation of isozyme patterns detected on zymograms; an appendix containing information on the buffer systems most commonly used for enzyme electrophoresis in starch, cellulose acetate, and polyacrylamide gels.
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 | Z731129 | Handbook of Pharmaceutical Biotechnology | This Handbook helps pharmaceutical scientists develop biotech drugs through a comprehensive framework that spans the process from discovery, development, and manufacturing through validation and registration. With chapters written by leading practitioners in their specialty areas, this reference provides an overview of biotechnology used in the drug development process, covers extensive applications, plus regulations and validation methods, and features fifty chapters covering all the major approaches to the challenge of identifying, producing, and formulating new biologically derived therapeutics
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 | C2349 | High Performance Capillary Electrophoresis: Theory, Techniques and Applications | | pricing |
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 | Z701033 | Introduction to Macromolecular Crystallography | This book provides a comprehensive summary of the field of crystallography, fundamental theory of diffraction and properties of crystals, and applications in determining macromolecular structure. Without assuming a background in physics or math, book’s contents flow logically from basic principles to methods, such as those for solving phase problems, interpretation of Patterson maps, and the difference Fourier method. This introduction includes practical instruction on interpretation of data and methods for determining phases and also illustrates concepts throughout to clarify difficult ideas; integrates analogies to illuminate the text; equips the reader to employ the range of programs in crystallography.
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 | L3165 | Luminescence Biotechnology: Instruments and Applications | The dangers inherent in radioactivity-based methods have caused a shift towards luminescence measurements and visualization techniques. This up-to-date review covers the fundamentals of different luminescence-based assay systems, calculation methods, and instruments through the spectrum of applications and research advances. Topics include gene and protein assays, oxidative stress and tissue aging, applications of luminescent microspheres, and proton image analysis. This book clearly identifies the advantages of luminescence over other assay techniques, discusses its potential pitfalls, and illustrates the broad range of its utility.
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 | Z378488 | Membrane Analysis | | pricing |
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 | Z702293 | Methods for Affinity-Based Separations of Enzymes and Proteins | One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or downstream processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and cutting-edge methods.
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 | Z702404 | Microarray Quality Control | Microarray technology is a powerful tool for measuring gene expression, but the multiple steps involved, from sample selection to data analysis, are susceptible to potentially costly errors. Without sound quality control, experimental microarrays may produce useless or, even worse, misleading results. From experimental design to data processing, analysis, and interpretation, the text's emphasis remains on practical advice for each stage of planning and running a microarray study. Chapters cover: Quality of biological samples; Quality of DNA; Hybridization protocols scanning; Data acquisition; Image analysis; Data analysis.
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 | Z703486 | Microarrays & Microplates: Applications in Biomedical Science | This book describes the new microarray and microplate based technologies which facilitate large scale analysis of DNA sequence variants, mRNA molecules and proteins. It provides a review of the various methodologies being used to identify genetic variants and gene regulation and guides readers through both the application of these methodologies and experimental procedures.
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 | Z705020 | Microfluidic Techniques: Reviews and Protocols | Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer laboratory instructions, principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting.
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 | Z701238 | Microscopic Techniques in Biotechnology | Microscopic analysis of a visual image of a cell or cellular constituent, a biofilm or a tissue sample allows exploration of a variety of features. Standardized techniques detect and identify cellular organelles, specific nucleotide sequences, proteins, polymers and products of low molecular weight. In this book, the biotechnologist will find examples for the possibilities of a certain microscopic technique. He is also introduced into the structural features of a biological specimen, a subcellular compartment, a cellular tissue or a biofilm, as exemplified by a certain preparation and imaging technique. In a methods section, the user is introduced into instrumentation and will find a selection of preparation techniques.
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| | Z701378 | Molecular Biotechnology: Principles and Applications of Recombinant DNA, 3rd ed. | Completely revised, updated, and expanded, this edition covers the underlying scientific principles and deals extensively with the many industrial, agricultural, pharmaceutical, and biomedical applications of recombinant DNA technology. It offers greatly expanded coverage of directed mutagenesis and protein engineering, therapeutic agents, and genetic engineering of plants. Updated chapters reflect recent developments in biotechnology, such as cloning, gene therapy, and patenting and releasing genetically engineered organisms. Over 500 figures, including 200 that are new in this edition, illustrate all key concepts.
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 | M0566 | Molecular Fluorescence: Principles and Applications | Fluorescence spectroscopy is an important tool of investigation in many areas. Its advantage is extremely high sensitivity and selectivity - even single molecules can be detected - and it achieves a high spatial resolution and time resolution in combination with microscopic techniques or laser techniques, respectively. In biochemistry and molecular genetics, fluorescence spectroscopy has become a dominating technique. Together with the latest imaging techniques, fluorescence spectroscopy allows a real-time observation of the dynamics of intact biological systems with an unprecedented resolution.
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 | Z701661 | Molecular Modeling: Basic Principles and Applications, 2nd ed. | Written by experts, this book describes the basics to the extent that is necessary to reliably judge the results from molecular modeling calculations. It leads the reader from simple calculations on small molecules to the modeling of proteins and other relevant biomolecules. The beginner is guided through the first modeling experiment, and the routine user of modeling software provided with invaluable troubleshooting hints. “If currently popular ‘Dummies’ series of computer books were to publish a volume on molecular modeling this would be it” (Journal of the American Chemical Society). The authors provide practical hints for the application of available programs in computational chemistry.
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| | Z379786 | Mutation Detection: A Practical Approach | | pricing |
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 | Z704091 | NanoBiotechnology Protocols | Experts in nanomaterial synthesis and application describe the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The authors emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used a building block in larger assemblies. These materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology.
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 | N6398 | Nucleic Acid Electrophoresis | Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA). Various techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described in detail. Required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers addresses, helpful Internet resources as well as specific applications are presented.
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 | Z373583 | Platelets: A Practical Approach | This book describes state-of-the-art techniques for the experienced researcher, both in academe and industry. The seventeen chapters cover techniques from basic platelet preparation to the latest procedures such as monitoring calcium levels in single cells, tyrosine phosphorylation, and PER.
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 | Z700827 | Pocket Guide to Biotechnology and Genetic Engineering | Biotechnology and genetic engineering are the key technologies of the 21st century. They allow the findings in cell biology and genetics, biochemistry and microbiology, biochemical engineering and bioinformatics to be applied to health care, agriculture, food production, environmental protection and alternative production methods for chemicals. This pocket guide provides a broad overview of the relevant facts on products, methods and applications, while discussing the opportunities and risks of these new technologies.
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| | Z357502 | Practical Protein Electrophoresis For Genetic Research | This methodologically oriented book includes principles, gel interpretation, troubleshooting, isozyme nomenclature, data collection and analysis, and genetic and statistical procedures for studies in starch and polyacrylamide gels.
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 | P7492 | Proteins: Biochemistry and Biotechnology | This book provides an overview of the various medical, diagnostic and industrial uses of proteins. It has evolved from an earlier text “Protein Biotechnology” and includes the latest developments in the field and offers a varied selection of relevant case study material. Initial chapters focus upon issues such as protein structure, folding, stability, purification and characterization. Later chapters detail the industrial production of proteins, and their applications in medicine, analysis and industry. It presents a comprehensive review of proteins and enzymes used in the diagnostic, therapeutic and industrial areas.
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 | P4617 | Proteome Revisited: Theory and Practice of all Relevant Electrophoretic Steps | This covers electrophoretic steps leading to proteome analysis, i.e. isoelectric focusing (including immobilized pH gradients), SDS-PAGE electrophoresis and finally two-dimensional maps. It is a collection of modern methodologies leading to successful fractionation, analysis and characterization of every polypeptide spot in 2-D map analysis. It includes chapters on sophisticated mass spectrometry developments and navigating important databases. It contains several as yet unpublished protocols, correcting some of the existing ones and showing the pitfalls and limitations of others.
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| | Z357391 | Protocols for Gene Analysis: Methods in Molecular Biology, Vol. 31 | A set of techniques is presented for the basic manipulation and mutagenesis of the cloned sequences. Also addressed are genome structure, sequence variation, gene expression, protein function, and methods used to take the step from the first gene to those with which it interacts.
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 | Z730424 | Pyrosequencing Protocols | This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method. Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5–7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8–13, and the important aspects of data storage is discussed in Chapter 14. This text both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.
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 | Z700975 | Radioisotopes in Biology 2nd ed., A Practical Approach | This new edition is completely updated and a reference source for using radioactivity in the bioscience laboratory. It starts with three chapters that provide the reader with a thorough understanding of the issues to be considered in choosing a radioisotope and in its safe use. The key techniques of autoradiography and scintillation counting for the detection and quantitation of radioactivity are then covered in full practical detail. Four other chapters describe methods and approaches for the radiolabelling of nucleic acids and proteins, subcellular localization and identification of macromolecules, immunoassay and pharmacological techniques. Summaries of the legislative requirements for using radioisotopes in the USA and European Union are included.
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 | Z375438 | Subcellular Fractionation: A Practical Approach | Provides step-by-step protocols and clear practical guidance on techniques needed in all cell biology laboratories. Comprehensively covers the wide range of separation methods and characterization procedures for all the major subcellular organelles: nuclei, mitochondria, chloroplasts, peroxisomes and membrane systems. Also covers the isolation of chromosomes, nucleoli and nucleoprotein complexes.
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 | Z730106 | Translational Control in Biology and Medicine | The new edition of this successful monograph has been both updated and broadened. Since the previous (second) edition was published in 2000, the structures of the bacterial and eukaryotic ribosomes have been published, advancing our basic understanding of translation and mechanisms involving protein and RNA regulators. In addition, as the title indicates, this edition has a new focus on the role of translational control in human development and disease. This book, with 30 chapters written by experts in the field, is essential reading for anyone interested in the process of translation, its regulation, and how its failure can be the cause of disease.
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 | V1888 | Visualization of Receptors in situ: Applications of Radioligand Binding | Radioligand binding in situ is the most sensitive method for detecting available receptors. But the success and reliability of this approach are determined by a number of technical requirements and constraints. This book combines theory and practice. It covers procedures such as radioligand and tissue preparations, in vitro assays, autoradiographic emulsion handling and image analysis. It also provides detailed protocols and uses parallel columns to convey practical comments and advise, often not encountered in original research papers.
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