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Cell Culture Books


Best Books for the Best Minds



Our Cell Culture books provides a variety of methods resources that include topics such as stem cells and gene therapy.

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Z703036Adult Stem Cells This comprehensive review of our current understanding of adult stem cell types and their regulation examines the derivation, characterization, and utility of adult stem cells found in skeletal muscle, dental pulp, prostate, kidney, the eye, inner ear, and other tissues. The authors pay special attention to adult stem cell plasticity and their utility in normal tissue homeostasis, as well as their applications in regenerative medicine.
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A2844Animal Cell Biotechnology: Methods And Protocols Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection, cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity.
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Z730580Animal Cell Biotechnology: Methods and Protocols, 2nd ed. This second edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation. Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques.
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Z702943Animal Cell Culture and Technology, 2nd ed. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed. New topics are included to reflect the latest developments and trends in the field. They cover the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation and the humanization of antibodies for therapeutic use
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A0687Animal Cell Culture: A Practical Approach, 3rd ed. This edition includes new or updated chapters on: cell authentication; serum-free culture; apoptosis assays; FISH; genetic modification; stem cell assays; 3-dimensional culture; tissue engineering; cytotoxicity assays; scaling-up of cell culture. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible
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 Z361798Animal Cells: Culture and Media: Essential Data Series This book presents the most relevant data available on culture techniques in a concise form which can fit into a lab coat pocket. It does not aim to replace the excellent basic texts on cell culture that are available, but to complement them by being a ready reference.
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Z702919Basic Cell Culture Protocols 3rd ed. This third edition offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. This book should be in every cell culture laboratory and be of use to all who use cell cultures in research
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Z378186Basic Cell Culture Protocols, 2nd ed.: Methods in Molecular Biology, Vol. 75 pricing
B2183Basic Cell Culture: A Practical Approach, 2nd ed. This book is a guide to all those areas which are currently basic to the performance of animal (including human) cell culture. Experienced and novice researchers should find this book useful as a source of reference, when entering a new field, or when setting up a new laboratory.
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Z378496Cell Biology: A Laboratory Handbook, 2nd ed. A superb handbook, this four-volume set should be a required resource for any biology or biochemistry research facility. Contributors are world-renowned in their fields, and the content has been peer-reviewed. The 260 chapters are uniformly edited and beautifully illustrated; 40% of the material is new to this edition. As an encyclopedic manual of up-to-date instructions for cell biology techniques and transitions to molecular biology, it's the next best thing to having the expert at your side.
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Z701750Cell Biology: A Short Course, 2nd ed. Taking an "essentials only" approach, this text, a completely revision of the earlier textbook From Genes to Cells, tells the story of cells as the unit of life in an accessible, user-friendly manner. The authors combine their expertise to skillfully present key points and illustrate them with clear diagrams and numerous examples from current research. This book offers: a unified presentation of cell biology in both medicine and industry with extensive cross-referencing; copious illustrations that make material clear and easy to understand; numerous boxed inserts that tie concepts to real-world research and developments in medicine and industry; a detailed, complete description of intercellular signaling.
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Z705292Cell Culture Technology for Pharmaceutical and Cell-Based Therapies This reference presents a comprehensive overview of the state-of-the-art in cell culture technology. Each expertly written chapter covers research, principles, and equipment currently affecting advances in the discovery of genes, host cell selection, cloning and gene amplification, bioreactor design, protein purification, optimization and scale-up, product quality, and facility design--showcasing the importance of genomic and cell-based technologies in the pharmaceutical industry. Providing clear sections on aspects of cell biology and biochemical engineering used in pharmaceutical process development and manufacturing, this source describes manipulations and the genetic engineering of cells for the production of biotechnology products
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Z340669Cell Growth, Differentiation and Senescence: A Practical Approach This book provides a combination of succinctly expressed basic concepts of cell growth and cell death with detailed instructions and protocols on how to accurately measure these processes. Practical instructions are accompanied by explanatory material, which allows a researcher to choose which protocol is best for their purpose. Methods outlined range from simple techniques, such as autoradiography and cell staining, to more complex techniques, such as flow cytometry.
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Z718890Cell Growth: Control of Cell Size Recent breakthroughs in the field of cell growth, particularly in the control of cell size, are reviewed by experts in the three major divisions of the field: growth of individual cells, growth of organs, and regulation of cell growth in the contexts of development and cell division
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Z702528Cell Growth: Control of Cell Size Recent breakthroughs in the field of cell growth, particularly in the control of cell size, are reviewed by experts in the three major divisions of the field: growth of individual cells, growth of organs, and regulation of cell growth in the contexts of development and cell division. This book is an introductory overview of the field and should be adaptable as a textbook.
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Z703877Cell Migration: Developmental Methods and Protocols A collection of classic, novel, and state-of-the-art methods for the study of cell migration in cultured cells, different model organisms, and specialized cells in normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The protocols follow the Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls
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C0983Cell Movements: From Molecules to Motility, 2nd ed. Interiors of cells are busy with purposeful movement, as vesicles are transported from one location to another and chromosomes align and then separate during cell division. What powers these movements? How are they controlled and made responsive to external stimuli? This book describes how complex movements can arise from properties and behaviors of biological molecules. Biophysical parameters are highlighted, emphasizing importance of diffusion, viscosity, and mechanical forces in determining cellular responses.
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C9862Cell Physiology Sourcebook: A Molecular Approach, 3rd ed. This authoritative book gathers together a broad range of ideas and topics. It provides clear, concise, and comprehensive coverage of cellular physiology from fundamental concepts to more advanced topics and contains substantial new material. The book includes chapters on sensory transduction, the physiology of protozoa and bacteria, the regulation of cell division, and programmed cell death. Features include eight new chapters on such topics as membrane structure, intracellular chloride regulation, transport, sensory receptors, pressure, and olfactory/taste receptors; broad coverage of both animal and plant cells.
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C4724Cell Separation: A Practical Approach The successful separation of specific cells requires not only a knowledge of potential methods, but also detailed protocols that are known to work well in practice. This book is unique in that it brings together the best methods currently used for separating cells. For each approach, the authors provide clear explanations of the theoretical basis of the procedures as well as practical details of the technique. All researchers for whom cell separation is the starting point for their experimental work should read this book. As such, it is an invaluable laboratory manual for cell biologists, biochemists, molecular biologists, and physiologists.
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C9605Cell and Tissue Culture for Medical Research This is a collection of fundamental and specific applied procedures in cell and tissue culture for clinical research including those that form the basis for the new medical techniques of tissue engineering and gene therapy. It includes step-by-step protocols with troubleshooting tips, experimental examples to indicate expected results, and alternative procedures.
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Z382701Cell and Tissue Culture: Laboratory Procedures in Biotechnology This book introduces the reader to animal cell culture methods describing the key cells, core techniques, how to scale up the culture for commercial production, and regulatory aspects. It contains easy to follow, step-by-step protocols, with trouble-shooting tips and notes on time considerations. Other features include: Experimental examples to indicate expected results; Quick reference symbols such as safety icons with warning notes; A list of suppliers to allow easy access to laboratory products.
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C8485Cell-Cell Interactions: A Practical Approach, 2nd ed. The book comprises ten chapters written by experts in the field on cell-cell interactions. These areas have been selected for their breadth and relevance where these interactions have been shown to play a critical role in biological processes. Chapters include methodologies for specialized cell types, specialized domains of membrane contact, different developmental models and mammalian development.
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Z703990Culture of Animal Cells: A Manual of Basic Techniques, 5th ed. This fifth and fully revised edition of Culture of Animal Cells continues to provide the most comprehensive information on the techniques, equipment, principles, and practices of animal cell culture. It offers a complete background related to growth of animal cells in tissue culture, beginning with laboratory design, safety, and bioethics, then continuing to coverage of preparation through to contamination, molecular techniques, and troubleshooting.
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Z705314Culture of Cells for Tissue Engineering Specific applications of tissue engineering include studies of tissue development and function, investigating drug response, and tissue repair and replacement. This area is rapidly becoming one of the most promising treatment options for patients suffering from tissue failure. This book offers step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering. It offers a unique focus on tissue engineering methods for cell sourcing and utilization, combining theoretical overviews and detailed procedures. Each chapter covers: structural and functional properties of tissues, methodological principles, culture, cell selection/expansion, cell modifications, cell seeding, tissue culture, analytical assays, and a detailed description of representative studies
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C3612Culture of Epithelial Cells, 2nd ed. This edition, devoted to those cells that line the body protecting surfaces and secreting or absorbing material, provides revised descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. Each chapter is devoted to a different type of epithelium--epidermal, mammary, cervical, prostatic, oral, intestinal, pulmonary, hepatic, and urothelial. Freshney contributes a final chapter on a number of epithelia not covered in the main chapters, presenting them together in review form. Other features include alternate techniques coupled with critical comparative analyses and new chapters on the culture of oral, alveolar, intestinal, and urothelial epithelium.
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 C5228Culture of Hematopoietic Cells Provides scientists with step-by-step methods for easy use in the lab when cultivating and experimenting with blood cells. Covering a range of human and mouse cell types, this laboratory manual includes contributions from more than twenty leading investigators who offer their practical expertise on a wide range of topics. Features detailed instructions for reagent preparation and information on the latest specialized equipment and supplies.
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Z701696Culture of Human Tumor Cells This comprehensive resource provides stepwise protocols for the in vitro cultivation of the major types of human tumors. It is organized by specific tumor, discussing cultures for lung, gastric, colorectal, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. Detailed techniques for initiation, propagation, and characterization of established cell lines are presented. Drug treatment, selection, differentiation, assays for malignant cells, risks, and applications are discussed.
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 C5353Culture of Immortalized Cells pricing
 D7687DNA Transfer To Cultured Cells DNA Transfer to Cultured Cells describes updated methods and applications for transferring genetic material to cells in-vitro. Advances achieved in recent years to develop approaches for introducing DNA into cells have facilitated studies on the mechanism of expression of different important genes in the cells. Consequently, it has been possible to evaluate the role of different genes and their activation in a variety of important processes, such as growth and uncontrolled proliferation (cancer). Different cell types require different schemes for DNA transfer; this book describes these diverse methods, their application to specific cell types and their relevance to a biological problem.
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Z700495Dendritic Cells 2nd ed. This new edition substantially revises and expands the 1999 work with a section of color plates and new information on the interaction of dendritic cells and viruses, the role of dendritic cells in wound healing and atherosclerosis, delivery of apoptotic bodies, genetic engineering of dendritic cells, imaging, and practical aspects of clinical protocol development. The 45 chapters discuss the origin and molecular biology of dendritic cells, their interaction with other cells, and their roles in cancer, allergy, and infections. With the volume of literature on dendritic cells almost doubling every year, this book provides the most up-to-date synthesis of the literature, written by the very best authors.
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Z718327Development of Human Gene Therapy The idea of human gene therapy was accepted by the medical community and society at large long before believable clinical benefits began to emerge. In this book, some of the field’s most distinguished contributors chronicle the evolution of this momentous direction for medicine, illustrating how imaginative concepts shaped the development of technologies and brought the daring new idea to its current position of imminent practical success. This is a book designed to endure as clinical advances accumulate, a clear-eyed work of reference that will anchor the further development of this revolution in therapy. It is an essential addition to libraries of clinical medicine, biotechnology, and public policy, and a resource that no laboratory investigator with an interest in the biology of gene transfer should be without.
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C4849Dynamics Of Cell Division This volume focuses on the structural aspects of cell division, ranging from nuclear envelope breakdown to cytokinesis and partitioning of the cytoplasm. It examines spindle assembly and chromosome behavior in mitosis and meiosis, centromere and kinetochore structure and regulation, telomeres, the role of centrosomes, and mechanisms by which overall regulation is achieved. Written as a companion volume to Cell Cycle Control, this book provides an up-to-date account of developments in this exciting area of cell biology.
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E1404Embryonic Stem Cells: Methods and Protocols This book describes productive methods for using embryonic stem (ES) cells as in vitro developmental models for many cell and tissue types. These detailed protocols range from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries, to the generation of antibodies against very rare antigens, and to the identification of and characterization of protein and protein interactions. This will prove an invaluable resource for those actively using, or planning to use ES cells, to study fate choices and specific lineages.
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E2029Epithelial Cell Culture Protocols A team of experimenters and researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. The emphasis is on the analysis and assessment of epithelial cells, for example by looking at apoptosis and integrins, or by measuring membrane capacitance and confluence. Also described in detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria.
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Z369950Epithelial Cell Culture: A Practical Approach Cells in culture can sometimes organize into tissue-like structures. In vitro epithelial ¿tissue¿ serves as a useful model system for studying pharmacology, toxicology, and cell biology. Written by an international panel of researchers, this book details the methods for establishing such a culture and describes some interesting research applications.
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Z374067Epithelial Transport: A Guide to Methods and Experimental Analysis This volume contains an overview of the general principles underlying epithelial transport of ions, water and non-electrolytes, with guidance on practical approaches. Emphasis is placed on experimental techniques and methodologies.
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Z702617Gene and Cell Therapy: Therapeutic Mechanisms and Strategies, 2nd ed. This edition offers current data on delivery systems and therapeutic strategies, gene expression and detection, and disease targets and therapeutic strategies. It presents results and prospects in gene therapy clinical trials, other cell-based therapies and trials, and regulatory issues. Topics include optimization of non-viral gene therapeutics, gene therapy for cancer, and DNA vaccines.
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H6033Haematopoietic and Lymphoid Cell Culture This reference handbook describes the fundamental principles and procedures underlying the successful isolation of viable, functionally intact haematopoietic and lymphoid cells, and their maintenance as primary cultures. It provides technical information on the signals and mediators required for the differentiation and growth of these cells. Chapters discuss dendritic cells, T and B lymphocytes, monocytes and macrophages, NK and LAK cells, mast cells and basophils, hematopoietic differentiation of embryonal stem cells, and culturing of murine thymic explants.
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Z700509Handbook of Industrial Cell Culture: Mammalian, Microbial, and Plant Cells The authors describe the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.
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Z702927Human Cell Culture Protocols 2nd ed. A revised and updated collection readily reproducible techniques for culturing human cells. This edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine¿ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls
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H8786Human Cytogenetics: Constitutional Analysis, A Practical Approach, 3rd ed. This covers all basic aspects of human cytogenetic study other than malignancies and abnormalities. Since the 2nd edition, the largest new development has been the transition of fluorescence in situ hybridization to an essential tool for cytogenetecists. Its use in chromosome analysis is covered in detail. Another new technology described in detail is computerised image analysis. Conventional techniques have not been forgotten, with chapters on chromosome staining and banding techniques and meiotic studies. New authors take a fresh look at lymphocyte culture and prenatal diagnosis
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H8911Human Cytogenetics: Malignancy and acquired abnormalities, A Practical Approach, 3rd ed. The advent of molecular technologies has lead to a rapid acceleration in the cytogenetic study of malignancy and acquired abnormalities. As well as having a chapter devoted to molecular technologies (FISH, PRINS, and CGH), molecular methodology is emphasized in all chapters. There are three chapters on the specific methodology for the cytogenetic study of myeloid leukemia, acute lymphoblastic leukemia, and lymphomas and lymphoproliferative disorders. Also covered are chromosome instability syndromes, mutagen-induced chromosome damage in lymphocytes and the role of cytogenetics in the assessment of hematological disorders.
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Z701408Human Embryonic Stem Cells A discussion of all the key issues in the use of human pluripotent stem cells for treating degenerative diseases or for replacing tissues lost from trauma. On the practical side, the topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials. Regulatory issues are addressed in discussions of the ethical debate surrounding the derivation of human embryonic stem cells and the current policies governing their use in the United States and abroad, including the rules and conditions regulating federal funding and questions of intellectual property.
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Z704288Human Embryonic Stem Cells This text begins by addressing the basic biology of the cells, in the context of the other stem cell populations present in fetuses and adult organisms. Discussion then turns to how stem cells can be used to further the understanding of human developmental processes, which currently lags far behind that of model organisms. Several chapters describe attempts to generate stem cell derived specific lineages as potential tools for cell based therapeutics, and these are followed by discussion of the legal and moral issues which accompany this ground breaking research.
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Z701076Human Embryonic Stem Cells: An Introduction to the Science and Therapeutic Potential The social debate involving the creation of human embryos for stem cell therapy prompted the writing of this text. The emerging field of human embryonic stem cell biomedicine crosses many disciplinary boundaries - cell biology, reproductive biology, embryology, molecular biology, endocrinology, immunology, fetal medicine, transplantation medicine and surgery. This single reference provides basic information from these multiple disciplines as it pertains to the science of stem cells.
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Z652024Live Cell Imaging: A Laboratory Manual Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a CD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book.
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Z340766Lymphocytes: A Practical Approach Straight-forward protocols on preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification assays are contained in this book. It also covers advances such as the use of the gamma-interferon ELISpot assay and peptide-HLA tetrameric assay to quantify antigen-specific T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.
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 M9439Mechanics of the Cell Biological physics, the application of physics to provide an understanding of biological phenomena, is a new burgeoning subject. This explores the physics behind the architecture of a cell's envelope and internal scaffolding, as well as the properties of its soft components. It discusses the properties of individual flexible polymers, networks and membranes, and then considers simple composite assemblages such as bacteria and synthetic cells. Analysis is performed within a consistent mathematical framework, although readers can navigate from the introductory material to biological applications without working through the intervening mathematics.
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Z731137Medicines from Animal Cell Culture This book focuses on the use of animal cell culture, which has been used to produce human and veterinary vaccines, interferon, monoclonal antibodies and genetically engineered products such as tPA and erythropoietin. It also addresses the recent dramatic expansion in cell-based therapies, including the use of live cells for tissue regeneration and the culture of stem cells. It provides comprehensive descriptions of methods for cell culture and nutrition as well as the technologies for the preservation and characterization of both the cells and the derived products and describes the preparation of stem cells and others for use in cell-based therapies. The book includes experimental examples to indicate expected results and addresses the key issues of standardization and validation with chapters on GLP and GMP for cell culture processes
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N2909Natural Killer Cell Protocols A collection of classic techniques, new approaches to standard methods and unique models for manipulating and studying natural killer cells. Those include in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines and in vitro NK cell development. Techniques include vaccinia virus protein expression and calcium mobilization in cell conjugates.
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Z369969Neural Cell Culture: A Practical Approach Studying neural processes at the cellular or molecular level is immensely complex, but key breakthroughs have come through work with cells in culture. This a compilation of methods for preparing cultures from a wide variety of neural tissues. Key features are the illustrated dissection guides, methods for enriching distinct cell types, and optimization of viability and growth.
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Z704733Neural Development and Stem Cells, 2nd ed. This edition reflects the increase in our knowledge of the development of the nervous system. With an understanding that the regionalization of stem cells occurs early in development and that this bias appears to persist even after prolonged culture, Dr. Rao has added additional chapters on olfactory epithelial stem cells and retinal stem cells, both of which differ in their properties from ventricular zone and subventricular zone-derived neural stem cells. A new chapter on cell death summarizes the important changes in the death pathway that occurs as stem cells mature. Special attention is paid to the derivation of neural cells from embryonic stem cells. This book provides neuroscientists with a guide to stem cells in the nervous system, tracing with clarity the development of stem cells from differentiation to neurons, astrocytes, and oligodendrocytes.
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N3660Neural Stem Cells: Methods and Protocols Recognized experts describe the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their culture, and make it possible to define their properties in culture. The techniques are designed to help researchers identify endogenous stem cells as well as exogenous stem cells after transplantation in the brain. Protocols range from the simplest methods of isolation and characterization of neural cell properties to such sophisticated methods as characterizing gene expression, telomerase assays, and cell cycle kinetics.
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Z703001Plant Cell Culture Plant cell culture is an essential methodology in plant sciences, with numerous variant techniques depending on the cell type and organism. Plant Cell Culture: the basics provides the reader with a concise overview of these techniques, including basic plant biology for cell culture, basic sterile technique and media preparation, specific techniques for various plant cell and tissue types including applications, tissue culture in agriculture, horticulture and forestry and culture for genetic engineering and biotechnology.
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 Z358975Plant Cell Culture: A Practical Approach, 2nd ed. The second edition of this book incorporates a number of significant changes which reflect the impact of molecular biology on the plant sciences. The inclusion of new sections on cell selection for cold, salinity and toxin resistance will aid in understanding plant stress physiology at the molecular level. Methods for the establishment and manipulation of well-characterized cell culture systems, as well as general advice for protocol development, is presented.
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Z365599Plant Propagation by Tissue Culture, Part 1: The Technology Topics addressed include: methods available for in vitro propagation, variation in cultures and plants, the control of contaminants and diseases, genetic, environmental and tissue dependent factors affecting growth and morphogenesis in cultures, the nature and uses of plant growth regulators, and the components, preparation and uses of culture media.
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Z701831Plant Tissue Culture: Techniques and Experiments, 2nd ed. This second edition provides laboratory exercises in plant tissue culture that demonstrate major educational concepts. The experiments can be conducted with a variety of plant materials that are available year round from easily accessible sources. Students as well as new and experienced plant scientists in agriculture, university, and industry settings will find this concise manual to be of great value. The strength of this book lies in its descriptions of essential concepts, introduction to a wide range of major applications, and simple protocols to provide empirical demonstrations.
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P5738Principles of Tissue Engineering, 2nd ed. This edition combines prerequisites for a understanding of tissue growth and development, tools and theoretical information needed to design tissues and organs, and applications of tissue engineering to diseases affecting specific organ systems. Few experts would have predicted the explosion in knowledge of gene expression, cell growth and differentiation, variety of stem cells, new polymers and materials available, or introduction of the first tissue-engineered products into the marketplace. This provides detailed analysis of research on all of the major systems of the human body and has new chapters on fetal tissue engineering and the universal cell.
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Z379980Recombinant Proteins from Plants: Production and Isolation of Clinically Useful Compounds A comprehensive and detailed handbook of essential techniques for producing recombinant proteins in plant hosts. Focuses on large-scale, cost-effective production, but includes sufficient detail to permit methods development in single-cell expression systems. The authoritative panel of contributors also discuss techniques they believe will become more important in the future: efficient transformation with Agrobacterium, optimizing stability of plant-expressed proteins, and the immunotherapeutic potential of such proteins.
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R3401Role of Catechol Quinone Species in Cellular Toxicity This book provides in-depth description and analysis of the potential of catecholamine-quinones to destroy nerves in which they are synthesized, mechanisms by which this occurs, sites at which intervention is possible, and role of astroglia. Insight is gleaned from mechanisms of action of several neurotoxins, while analysis of mechanisms of chemically-related tetrahydroisoquinolines provides recognition of the neurotoxic potential of endogenously formed orthoquinones from catecholamines. This book is of value to those investigating catecholamine mechanisms of action, neurotoxins, Parkinson's Disease and other neurodegenerative disorders, carcinogenicity, particularly melanoma, and other cancers like leukemia.
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Z701726Short Protocols in Cell Biology Providing condensed descriptions of more than 500 methods compiled from "Current Protocols in Cell Biology", this text explores cell biology in an easily accessible, hands-on format. This is an authoritative and indispensable guide for all life scientists and researchers. The text is designed to provide quick access to step-by-step instructions for the essential methods used in every major area of cell biological research, and it contains methods from every aspect of cell biology everything needed to study the basic structure and functions of cells at both the molecular and cellular levels
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Z730254Stem Cell Tools and Other Experimental Protocols : Methods in Enzymology Volume 420 This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook” and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional” and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives.
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Z701475Stem Cells Handbook This survey the "state-of-the-science" of stem cell biology explains the origins of stem cells and describes how they function, how they can cause illness, and how they might be employed to cure or ameliorate disease. In particular, the authors discuss the roles of stem cells in development and organogenesis, in normal tissue turnover, in the repair response to injury, and in carcinogenesis. They also explain how to obtain stem cells from different organs, culture them in vitro, and manipulate them for therapeutic use.
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Z705268Surrogate Tissue Analysis: Genomic, Proteomic and Metabolomic Approaches This book describes initial applications and considerations for "omic" technologies in the field of surrogate tissue analysis. Highlighting important issues to consider when conducting profiling studies to identify novel biomarkers, the first section covers transcriptional approaches in surrogate tissues. The second section focuses on proteomic and protein-based methods for identifying markers in surrogate tissues, highlighting immunoassay and mass-spectrometry approaches for assessment of proteins in serum and other fluids, with a focus on the implications of protein-based biomarkers for detecting and monitoring early stages of cancer. The third section explores metabolomic approaches along with other novel molecular screens that can be applied in surrogate tissues to find biomarkers.
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Z703907Tissue Engineering: Essentials for Daily Laboratory Work This practical book provides a fundamental insight into the complex world of tissue development and artificial cell culture using tissue engineering. The introductory chapters cover basic cell biology and cellular development as well as cell culture, with a main emphasis on ways of differentiating tissue and the critical evaluation of the properties of maturing tissue constructs. The authors also focus on the use of stem cells from the most varied sources in tissue engineering. The whole is rounded off by an exceptionally wide-ranging glossary containing some 1,000 key words from the fields of cell biology, cell culture development and tissue engineering.
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Z702498Tumor Necrosis Factor: Methods and Protocols A comprehensive collection of readily reproducible techniques for TNF production, characterization, mutagenesis, and detection in biological specimens. Also included are several in vitro assays and animal models for studying the role of TNF in various TNF-related diseases and in cancer. The collection offers molecular and cellular biologists, pharmacologists, and toxicologists a diverse set of versatile, cutting-edge tools for illuminating the pathophysiological roles of TNF in disease and for identifying new drugs.
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T2442T Cell Protocols A compilation of step-by-step techniques for the study of T cell developmental biology and their subsequent activation. It includes treatment of both fundamental and novel experimental protocols for the analysis of the role T cells play in immune function and in the prevention and cure of human diseases.
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