 | Z702595 | A-β Metabolism and Alzheimer’s Disease | This book explores the aggregation of amyloid-β peptide in the brain which plays a primary role in the pathogenesis of Alzheimer’s disease. It provides current information regarding generation, degradation within the brain, and transport out of the brain.
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 | Z705187 | Adrenergic Receptors in the 21st Century | An authoritative review of the current state-of-the-art understanding of the structure and function of the adrenergic receptor subtypes, as well as of the role played by these receptors in physiological and pathophysiological settings. Topics range from structure-function studies and the imaging of adrenergic receptors to the use of genetically altered mouse models and pharmacogenomics. Highlights include a survey of the knockout and overexpressed mouse models, a review of the new ways that adrenergic receptors can signal, and the effects of polymorphisms on clinical outcomes and on potential gene therapy applications. The side-by-side comparison of all the adrenergic receptors (a1, a2, and b) provides the reader with an excellent survey of the field, including the rationale for designing better drugs to control blood pressure and heart function.
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 | Z703702 | Amyloid Precursor Protein: A Practical Approach | Amyloid precursor protein (APP) is an extensively studied transmembrane protein, and its derivative, amyloid β-protein, has been implicated as the primary cause of Alzheimer's disease. In the search for an effective treatment, APP is a unique model protein that illustrates the wide array of techniques available for characterization. This book presents a variety of biological techniques to elucidate the structure and function of this transmembrane protein. Each technique is presented with detailed protocols to achieve reproducible results and provide a framework for studying other membrane proteins. These protocols also form the core of the authors' approaches to elucidating the function of APP. This book contrasts methods to explain how the development of biological techniques affects the way molecular targets are examined.
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 | A8721 | Angiogenesis Protocols | Although angiogenesis-formation of new blood vessels from pre-existing ones-has emerged as a target for the treatment of many disorders, fundamental aspects remain poorly understood. To address those needs, this book is a collection of key techniques for assessing the formation of new blood vessels in both whole tissue and cell culture. The methods range from low-cost in vitro assays to cutting-edge in vivo models. Included are details of the associated techniques for the isolation, identification, and culture of microvessel endothelial cells from human adipose tissues, and for the transfection and transduction of primary human endothelial tissues.
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 | A9843 | Apoptosis Detection and Assay Methods | This book provides cutting-edge methods in apoptosis detection, and broad in-depth coverage of current apoptosis research methodologies. Step-by-step protocols for apoptosis detection, such as use of the apoptotic cell surface marker annexin V assay, single strand DNA antibody assays, caspase substrate assays, and several improved ways of detecting oligonucleosomal fragments are detailed. This book contains updated, comprehensive reviews of apoptosis technology development and applications, including the FACS method, in vitro biochemical approaches, and newly emerging yeast methods.
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 | Z702668 | Apoptosis Methods and Protocols | A collection of cutting-edge techniques for detecting and quantifying apoptosis, understanding its biochemistry, and for identifying the genes and proteins that regulate and carry it out. Described in detail, these reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. This book constitutes a key technical reference to the significant methodologies used in the field and powerful tools to illuminate the phenomenon of programmed cell death.
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 | A6975 | Apoptosis Techniques and Protocols, 2nd ed. | This edition describes in detail state-of-the-art techniques for studying neuronal cell death. These methods solve a variety of research problems that include the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of pro-apoptotic proteins in situ. Included are hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays.
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 | Z700711 | Apoptosis and Autoimmunity: From Mechanisms to Treatments | This is the first comprehensive book about the relationship between apoptosis and autoimmune diseases. It offers a unique up-to-date overview on research results on the defective execution of apoptosis and the incomplete clearance of apoptotic cells. The molecular and cellular mechanisms involved are described in detail. As a possible consequence of apoptotic dysfunction, the development of severe autoimmune diseases (e.g., rheumatoid arthritis, systemic lupus erythematosus) is discussed. An outlook on future research topics includes the evaluation of novel therapeutic strategies.
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 | Z340677 | Apoptosis: A Practical Approach | This text provides conceptual outlines and detailed procedures for basic and advanced studies of cell death by apoptosis. Chapters on the recognition of apoptosis as distinguished from necrosis and nonspecific cell DNA damage are followed by a systematic examination of the established and the principal noval methodologies utilized in apoptosis research.
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 | A7101 | Apoptosis: the Molecular Biology of Programmed Cell Death | Apoptosis is a regulated form of cell demise that can be induced or blocked by groups of specific stimuli. It is thought critical to maintenance of homeostasis and is implicated in lowering susceptibility to tumor growth. Conversely, oversensitivity to apoptotic triggers can cause cells to be lost inappropriately from tissues, as in neurodegenerative diseases. This book examines molecular pathways regulating apoptosis, progressing from the nematode worm through Drosaphila to more complex pathways evident in mammalian cells. Differences in cell death pathway within specific tissues are addressed, as are different genes that may act to regulate progressive steps.
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 | B8305 | Beyond Neurotransmission | This book provides the foundations for understanding the cellular and molecular basis for neuromodulatory effects. It illustrates key examples of the roles played by neuromodulation in sensory processing, neuromuscular transmission, generation of motor behaviors, and learning. The book also underscores the importance of information processing by the nervous system in future studies. By spanning the levels of analysis from sub-cellular mechanisms through cellular properties and neuronal systems to behavior, the book provides a framework for understanding this field.
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 | B6308 | Biochemistry of Cell Signalling | This will serve as a reliable map through the maze of cell signaling pathways and help the reader understand how malfunctions in these pathways can lead to disease. Part 1 describes the machinery of signal transduction starting with the properties of signals, receptors (including receptor activation), regulators, and the molecules that link receptor and regulator. Part 2 describes the implementation of the signaling cascades focusing on the effect on gene transcription. Part 3 studies the global cellular regulatory programs for the control of cell growth and proliferation. Part 4 considers loss of regulatory control and its consequences with respect to the molecular basis of cancer.
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 | Z701297 | Biochemistry of Signal Transduction and Regulation, 3rd ed. | This book has become the "gold standard" in the field of signal transduction and offers unmatched quality from the pen of a single author. The author succeeds in balancing comprehensive coverage with a clear and didactic style. The new edition is completely updated. 50% of the content has been rewritten to incorporate recent findings, and many illustrations have been redrawn and are now presented in full color.
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 | B4556 | Biology of Phosphoinositides | This review of current lipid signaling research places emphasis on the integration of the use of lipid signals in signal transduction and membrane trafficking. The regulation of phospholipase C, phospholipase D, phosphoinositide-3-kinases, chloride channel conductance by inositol (3,4,5,6) tetraphosphate, and of cytoskeletal protein activity by inositol lipids are covered in depth. There is specific discussion of the PH and FYVE lipid domains that allow lipids to control the movement, location, and activation-state of membrane proteins.
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 | Z704717 | Calcium Signaling Protocols, 2nd ed. | This extensively updated and expanded second edition contains new chapters on the electrophysiological measurement of Ca2+ channel activity, enhanced coverage of confocal microscopy, and practical tutorials on two of the most common industrial high-throughput machines, the Fluormetric Imaging Plate Reader (FLIPR), and the FlexStation. All experimental chapters provide readily reproducible techniques for measuring the many facets of Ca2+ signaling. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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 | Z705225 | Calcium Signaling, 2nd ed. | In this edition, Putney focuses on those processes that generate calcium signals to compile the first comprehensive exploration of calcium signaling research from a methodological standpoint. This edition deals with methods for studying calcium from a variety of perspectives. Several chapters discuss calcium indicators and other tools, and look at microscopic and electrophysiological techniques, as well as other special methodological aspects of calcium signaling research. Other chapters examine the study of different systems, ranging from those found in yeast to those found in mammals, and several more are devoted to the cellular and molecular basis for calcium signaling, including explorations of receptors, calcium pumps, apoptosis, and drug delivery.
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 | C3362 | Calcium-Binding Protein Protocols, Vol. 2: Methods and Techniques | Researchers review protein chemistry and behavior of this class of proteins, and provide experimental techniques for studying it both in vitro and in vivo. This second volume focuses on techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding proteins in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium-binding proteins in cells.
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 | Z701785 | Caspases: Their Role in Cell Death and Cell Survival | The volume offers insight into the key research topics that involve caspases. Leading experts from various areas of biology and medicine have compiled this volume so that both the novice and the professional will use this as the main reference for caspases for years to come. The reader will learn about the role of caspases in apoptosis signaling, inflammation and cancer therapy while being updated on the methodology used to study caspases and the efforts of pharmaceutical research in targeting caspases.
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 | Z704806 | Cell Biology of Addiction | This monograph, written by experts in the field, is devoted to the molecular analysis of addiction pathways in the brain. It provides an intensive overview of the fundamentals, state–of–the–art advances, and major gaps in the cell and molecular biology of drug addiction within the broader context of neuroscience. Addiction research is a branch of neuroscience and psychology. The emphasis in this book is on hard science and the market for it will be found among research investigators and grad students within the field of neuroscience. The research presented is not only applicable to the study of drug abuse and addiction, but has clear implications for clarifying mechanisms of learning and memory, neuroadaptation, perception, volitional behavior, motivation, reward, and other disciplines of neuroscience.
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 | Z701750 | Cell Biology: A Short Course, 2nd ed. | Taking an "essentials only" approach, this text, a completely revision of the earlier textbook From Genes to Cells, tells the story of cells as the unit of life in an accessible, user-friendly manner. The authors combine their expertise to skillfully present key points and illustrate them with clear diagrams and numerous examples from current research. This book offers: a unified presentation of cell biology in both medicine and industry with extensive cross-referencing; copious illustrations that make material clear and easy to understand; numerous boxed inserts that tie concepts to real-world research and developments in medicine and industry; a detailed, complete description of intercellular signaling.
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 | Z701866 | Cell Cycle Checkpoint Control Protocols | A collection of cutting-edge techniques for studying the mechanisms underlying cell cycle regulation and checkpoint control. Using mammalian, yeast, and frog systems, these readily reproducible methods can be used to induce cell cycle checkpoints, detect changes in cell cycle progression, identify and analyze genes and proteins that regulate the process, and characterize chromosomal status as a function of cell cycle phase and progression. Each fully tested technique includes step-by-step instructions, an introduction explaining the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls
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| | Z369446 | Cell Cycle Control | This is the first book to cover the cell cycle field in the wake of groundbreaking research from the past five years. A historical look at cell cycle findings places this new knowledge into perspective and demonstrates the universality of cell cycle control, from the evolutionary process to cancer research and mitotic regulation.
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 | Z703753 | Cell Cycle Control: Mechanisms and Protocols | This collection of techniques for the study of the eukaryotic cell cycle and its key regulatory molecules includes overviews of cell cycle regulatory mechanisms in many major research organisms. Described in step-by-step detail, these reproducible methods enable fundamental research on well-defined cell cycle regulators and those more recently defined in yeasts, bacteria, plants, Drosophila, Xenopus, and mammals. The book offers all cell researchers indispensable techniques essential to understanding how normal cells divide and how this is altered in disease.
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 | Z702528 | Cell Growth: Control of Cell Size | Recent breakthroughs in the field of cell growth, particularly in the control of cell size, are reviewed by experts in the three major divisions of the field: growth of individual cells, growth of organs, and regulation of cell growth in the contexts of development and cell division. This book is an introductory overview of the field and should be adaptable as a textbook.
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 | Z719242 | Cell Imaging: Methods Express Series hard cover | Cell Imaging: Methods Express covers the key techniques that can be employed in any lab with access to cell imaging equipment, even if they do not currently specialize in imaging. It focuses on live cell imaging and light microscopy applications, but many of the techniques and approaches are equally relevant to the imaging of fixed specimens
The topics in this comprehensive research guide include:
Confocal versus widefield imaging; Transmitted light imaging; Photobleaching and photoactivation; Deconvolution and 3D reconstruction; Spectral imaging; Quantification and image analysis; Imaging calcium signals and activation of calcium binding proteins; Fluorescence Resonance Energy Transfer; Total Internal Reflection Fluorescence Microscopy; Correlative light-electron microscopy; Fluorescence Lifetime imaging; High Content and High Throughput imaging
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| Z719234 | Cell Imaging: Methods Express Series soft cover | Cell Imaging: Methods Express covers the key techniques that can be employed in any lab with access to cell imaging equipment, even if they do not currently specialize in imaging. It focuses on live cell imaging and light microscopy applications, but many of the techniques and approaches are equally relevant to the imaging of fixed specimens
The topics in this comprehensive research guide include:
Confocal versus widefield imaging; Transmitted light imaging; Photobleaching and photoactivation; Deconvolution and 3D reconstruction; Spectral imaging; Quantification and image analysis; Imaging calcium signals and activation of calcium binding proteins; Fluorescence Resonance Energy Transfer; Total Internal Reflection Fluorescence Microscopy; Correlative light-electron microscopy; Fluorescence Lifetime imaging; High Content and High Throughput imaging
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 | C1729 | Cell Neurobiology Techniques | This book details cutting-edge techniques and readily reproducible methods for establishing neural cell cultures, measuring enzymes and their inhibitors, and using quantitative autoradiography to study monoamine uptake sites and receptors in the brain. Additional methods cover the use of flow cytometry to study developmental neurobiology, applications of magnetic resonance spectroscopy (MRS) to human brain metabolism, and the study of drug metabolism.
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 | Z730416 | Cell Signalling, 2nd ed. | Cell Signalling presents a carefully structured introduction to this intricate subject, introducing those conserved features which underlie many different extra-and intracellular signalling systems. Starting with an overview of cell signalling, which highlights its importance in many biological systems, the book goes on to explore the key components of extracellular and intracellular signalling mechanisms, before examining how these components come together to create the signalling pathways, which are so crucial to the survival of all living organisms.
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 | Z705705 | Cell-Cell Interactions: Methods and Protocols | A versatile collection of readily reproducible cell–cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell–cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls
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| | Z369713 | Cellular Aging and Cell Death | This book bridges the rapidly growing fields of cellular aging and programmed cell death. It presents an authoritative overview of both fields, and demonstrates how both fields are critical to understanding the aging process. The role of apoptosis in a variety of age-associated diseases and disabilities is also covered.
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 | C9728 | Cerebral Signal Transduction | This book emphasizes the functional role of the various signaling pathways in memory, apoptosis and degeneration, depression, and drug dependence in both health and diseases states. With the ability to selectively remove, reduce or enhance specific components of the signaling pathways, the book highlights the varied signaling cascades, their crosstalk and possibilities for changes in gene expression underlying changes in brain function. It discusses the development of novel therapeutics targeting downstream events in the cascade of second, third and fourth messengers.
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| | Z362352 | Communication within Animal Cells | A clearly written textbook with many illustrations for students in advanced undergraduate and graduate courses. It puts into perspective a large body of knowledge about intracellular signaling systems.
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 | C1858 | Connexin Methods and Protocols | Volume 154 in Methods in Molecular Biology Series describes the best techniques to study cellular and molecular biology of connexins, as well as physiological properties. These methods are specifically designed to investigate the life of connexins from biosynthesis to assembly into gap junction channels, and include powerful assays to perform functional studies on these routes of cell-cell communication. It offers a collection of methods for studying the role of connexins in intercellular communication and human genetic disorders, as well as finding new therapeutics.
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 | Z701629 | Cytokine Handbook, 4th ed. 2 volume set | The fourth edition provides encyclopedic coverage of the molecules that induce and regulate immune responses. Now expanded to two volumes with contributions from more than 120 international experts, the scope of the book has been broadened to include a major emphasis on the clinical applications of cytokines. The early chapters discuss individual cytokines, chemokines and receptors. Additional chapters discuss the clinical implications and applications of cytokines, including cytokine gene transfer, antisense therapy and assay systems. This book is essential for anyone interested in cytokines and working in cancer biology, transplantation, infectious diseases, autoimmunity or bioinformatics.
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 | Z701467 | Cytokine Knockouts, 2nd ed. | Giamila Fantuzzi and a panel of experts have generated completely new chapters to reflect the use of many novel mouse strains and the hundreds of recent studies on cytokine physiology. Comprehensive reviews of the numerous often-surprising results obtained using cytokine knockout mice are provided, along with much important information about cytokine biology and physiology. For those not familiar with cytokine research, the authors present a critical discussion of the advantages and disadvantages of using cytokine knockout mice in various fields of research.
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 | C2606 | Cytokine Molecular Biology: A Practical Approach | This manual concentrates on molecular biology techniques for the study of cytokines, cytokine receptors, and cytokine driven processes. Topics include the cloning and expressing of cytokine genes, the analysis of cytokine mRNA by RT-PCR, cDNA array systems and in situ hybridization, the study of cytokine gene polymorphisms, the analysis of cytokine signaling via PC-specific phospholipase C and sphingomyelinases, the Jak/STAT and MAPK signaling pathways, and gene modification in transgenic and mutant mice.
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 | C2479 | Cytokine Network | Cytokines act in a complex network where one cytokine can influence production of, and response to other cytokines. This book covers the study of cytokine and cytokine receptor structure, elucidation of convergent intracellular signaling pathways, molecular genetics, and targeted gene disruption to 'knock-out' production of individual cytokines in mice. It is not a survey of individual cytokines, but a guide to the cytokine network covering genomics, signaling pathways, control of the immune response, and therapeutics.
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 | Z701432 | Cytokine Protocols | A collection of biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization
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 | C5231 | Cytoskeleton Methods and Protocols | Recent advances in experimental instrumentation, reagents and imaging technology have expanded the range of tools available for the study of the cytoskeleton. This book details reproducible methods for investigating cytoskeleton structure and function utilizing diverse model systems in a variety of organisms. Microscopy applications include digital-video microscopy and computer-assisted systems for the evaluation of cell motility and morphology. Help notes and tips accompany each protocol and provide additional information.
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 | Z340723 | Cytoskeleton: Signalling and Cell Regulation: A Practical Approach | | pricing |
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 | Z718211 | Discovering Neurons: The Experimental Basis of Neuroscience | Discovering Neurons is a collection of investigative laboratory experiments designed for the advanced undergraduate and the first or second year graduate student. In its three major sections, neurons are studied as: individual cells, including topics such as intracellular recording, immunocytochemistry and HPLC forming connections, with experiments on tract tracing, information coding and LTP, among others as coordinators of behavior, through approaches such as pharmacology, lesioning and ethology.
The fourth section focuses on techniques applicable to several experiments in the collection, including electrophysiology recording, surgery, anesthetics and staining methods. The experiments introduce students to the techniques used in today's leading neuroscience research labs. They have been thoroughly tested in the student laboratory and are written and designed to be easily integrated into existing neuroscience courses of any length or emphasis. This is a unique volume that is an important resource for all teachers of neuroscience.
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 | Z703621 | Drug Metabolism and Transport: Molecular Methods and Mechanisms | A compendium of proven approaches for studying the bioactivation, detoxification, tissue distribution, and elimination of xenobiotics in the metabolism and/or transport of various chemicals. Authors address several of the major drug metabolizing systems, including the cytochrome P450 family, flavin-containing monooxygenases, glutathione, S-transferase, glucuronidation, N-acetylation, and sulfotransferases. Additional chapters present novel approaches to the study of: signaling pathways in the regulation of drug metabolism enzymes, how the modulation of thiols and other low molecular weight cofactors can alter drug metabolism, and how modulation of drug metabolism pathways can influence antiviral therapy.
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 | Z705101 | Epidermal Growth Factor: Methods and Protocols | A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols format offers step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls
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 | E0641 | Extracellular Matrix Protocols | Leading bench-top experimentalists present state-of-the-art methods for probing the extracellular matrix (ECM), its mechanisms of assembly and how it influences cell behavior. These highly detailed biochemical, biophysical, genetic, molecular biological, and cell biological techniques have been optimized for study of ECM structure and assembly, mechanisms of cell–ECM interactions, and molecular genetics of ECM proteins and defects in human disease.
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 | Z702153 | Extracellular Matrix and the Liver: Approach to Gene Therapy | This book addresses the basic science of the extracellular matrix and discusses new strategies for the treatment of cirrhosis of the liver, with a primary focus on possible gene therapy approaches. The chapters are divided into six sections as follows: Basic science of extracellular matrix; Cells responsible for extracellular matrix production; Activation mechanism of hepatic cells and signal transduction; Basic science for extracellular matrix metabolism including enzymes and their inhibitors; Matrix metalloproteinases and tissue inhibitors for matrix metalloproteinases; New strategies for the treatment of liver cirrhosis.
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 | Z379697 | Flow Cytometry Protocols: Methods in Molecular Biology, Vol. 91 | This volume includes details on new methods that rely on recently developed instrumentation, without short-changing the time-tested standard techniques. Methods are given for detecting intracellular cytokines, nuclear antigens in leukemia and solid tumors, and cyclins. Protocols are included for performing functional studies like assessing viability, membrane potential, oxidative metabolism, drug resistance, and pharmacokinetics. Nucleic acid methods include cell cycle analysis, chromosome sorting, solid tumor ploidy analysis, apoptosis, and a flow cytometric application of FISH.
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 | F2799 | Free Radical and Antioxidant Protocols | Seventy international experts join forces to present step-by-step instructions for a wide range of cutting-edge assays to study primary and secondary oxidative stress. Techniques for assays on free radicals, antioxidant enzymes, antioxidant vitamins and nonvitamins, trace metals, and cellular/membrane transport are included. They take advantage of new instrumentation such as probes, photon counting, chemiluminescence and caged compounds with an emphasis on HPLC. The methods are adaptable to a wide range of applications.
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 | Z701440 | Fundamental Neuroscience, 2nd ed. | The demand is great for a textbook that both introduces graduate students to the full range of neuroscience, from molecular biology to clinical science, but also assists instructors in offering an in-depth course in neuroscience. This second edition accomplishes this and more. The revised text features over 25% new material including new chapters, illustrations, and a CD-ROM containing the figures from the text. Logically organized into 7 sections, this edition includes numerous text boxes with concise, detailed descriptions of specific experiments, disorders, methodological approaches, and concepts. It is well-illustrated with over 850 full color figures which are included on the accompanying CD-ROM.
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 | Z703761 | G Protein Coupled Receptor-Protein Interactions | This exciting volume elucidates how GPCRs organize signal transduction and control intracellular activities. Chapters written by the recognized expert in each respective topic offer state-of-the-art updates on the principles and technology, as well as stepwise protocols for methods currently applied to the analysis of protein-receptor interactions
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 | Z704148 | G Protein Coupled Receptors Handbook | Authors describe the knowledge of GPCR receptor structure and function, the different mechanisms involved in the regulation of GPCR function, and the role of pharmacological chaperones in GPCR folding and maturation. They also present new findings about how GPCR dimerization/ oligomerization modifies the properties of individual receptors and show how recent developments are leading to significant advances in drug discovery, such as the detection of ligands for orphan GPCRs. Also discussed are the most recent developments that could lead to new drug discoveries: role of GPCRs in mediating pain, development of receptor-type selective drugs based on the structural plasticity of receptor activation, and identification of natural ligands of orphan GPCRs (deorphanization) as possible drug targets.
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 | M5565 | G Protein Pathways Part B, G Proteins and their Regulators, Methods in Enzymology Vol 344 | This volume covers topics such as the structure and identification of functional domains of G proteins, and activation of G proteins by receptors or other regulators. The text takes an integrated approach to studying common experimental questions at many different levels related to G proteins. Methods related to G proteins using molecular modelling, systems biology, protein engineering, protein biochemistry, cell biology, and physiology are all accessible in the same volume.
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 | M5440 | G Protein Pathways, Part A Receptors, Methods in Enzymology Vol 343 | G Protein Pathways is the first of three volumes examining the nature of heterotrimeric G proteins. The text takes an integrated approach to studying common experimental questions at many different levels related to G proteins. Methods related to G proteins using molecular modeling, systems biology, protein engineering, protein biochemistry, cell biology, and physiology are all accessible in the same volume.
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 | Z701416 | G Protein Signaling: Methods and Protocols | The involvement of G protein coupled systems in a range of biological processes in organisms from yeast to man has generated a large degree of interest. Due to the interdisciplinary nature of G protein coupled systems the types of techniques used to study their functions varies considerably. The methods contained in this text contain complementary biochemical, molecular biological and cell biological approaches to addressing questions so that they can be studied from multiple perspectives and adopted by different types of laboratories. These methods are of interest to scientists studying physiological roles of G protein systems, the signal transduction systems associated with these G proteins systems as well as the molecular nature of G proteins themselves.
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 | G1540 | G Protein-Coupled Receptors | Covering recently developed methods in membrane-bound receptors, this book emphasizes receptor structure and function, knowledge of which is essential to the study of signal transduction. G Protein-Coupled Receptors has utilized contributors from domestic and international sources, providing a broad base of knowledge. Some topics covered are the regulation of receptors; phosphorylation of G-protein-coupled receptors and ion channel receptors; desensitization studies using electrophysiological or confocal microscopic methods and the search for endogenous ligands for orphan receptors.
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 | G2415 | G Proteins: Techniques of Analysis | This book covers essential methods - with a commitment to those techniques of proven and current utility. This title includes expression and functional analysis of G proteins; evaluation of covalent modifications and other regulatory phenomena; and, mapping pathways established among receptors, G proteins, and effectors. Includes contributors from key institutions. Each author offers clear instructions to establish a synthesized, concise and consistent approach to each chapter, beneficial to all.
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 | Z718777 | Genes & Signals | Genes & Signals analyzes gene regulation from a new perspective. The first chapter describes mechanisms found in bacteria, and two subsequent chapters discuss which of these is most highly exploited in higher organisms. A final chapter relates these molecular strategies to other enzymatic processes, including those involving kinases, RNA splicing enzymes, proteases, and others. A general theme emerges, one that proposes how a rather restricted set of signals and enzymatic functions has been used in evolution to generate complex life forms of different types.
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 | G6292 | Genes and Signals: Imposing Specificity on Enzymes by Recruitment | Many cellular functions require the orderly switching on and off of gene transcription, often in response to signals from other cells or the environment. This concise, attractively illustrated volume is a textbook on gene regulation and its importance in development and evolution. But the book also argues that understanding how transcription-related enzymes are controlled will reveal much about the regulation of enzymes in other biological processes as well.
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| | G4789 | Growth Factors and Receptors: A Practical Approach | Provides comprehensive protocols for studies of growth factors and their interactions with receptors. Describes important novel approaches, such as the use of RNA aptamers as receptor antagonists, and the development of receptor superantagonists. Covers a wide range from simple analytical techniques to sophisticated in vivo applications including:
• RT-PCR and immunocytochemistry for detection of growth factors and receptors
• Production and purification of recombinant growth factors and receptors
• Labeling of growth factors for binding studies
• Yeast two-hybrid assay of protein-protein interactions
• Analysis of important factor domains using chimeric proteins
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| | Z350044 | Growth Factors: A Practical Approach | This manual is a step-by-step guide to studying growth factors from the initial search for and characterization of new activities, to sophisticated in vivo analysis and molecular biology approaches, such as DNA cloning.
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 | Z701920 | Growth and Lactogenic Hormones: Neuroimmune Biology Vol. 2 | For seventy years evidence has accumulated documenting the existence of a bi-directional communication network between growth hormone and the immune system. In the past twenty years there has been a tremendous proliferation of information detailing the workings of the growth hormone and insulin-like growth factor axis. A multitude of growth factors and binding proteins have been identified. More evidence supporting the important role of the growth hormone IGF network in the well functioning of the normal immune system has been documented. The challenge today is not to prove, but to understand, the neuroimmune regulatory role of GLH in its entire complexity.
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 | Z378070 | Guidebook to Protein Toxins and Their Use in Cell Biology | Toxins are presented in groups according to their modes of action and cellular effects. Groups include membrane-permeabilizing toxins, toxins affecting signal transduction and protein synthesis, cytoskeletal toxins, toxins affecting membrane traffic, toxins that target ion channels, and presynaptic toxins.
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 | Z379611 | Highly Selective Neurotoxins: Basic and Clinical Applications | Provides the background knowledge necessary to obtain a predictable experimental outcome with drugs that are used to destroy selected neurons. Each chapter discusses the mechanism of action of a neurotoxin and the limitations of its experimental use. Includes a discussion of xenobiotics, drugs of abuse, and medicines with neurotoxic potential. Provides insights into neuron function and response to chemical insults.
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 | Z704342 | Human Nervous System, 6th ed. | Authors take advantage of recent advances in neurobiology and molecular biology to include new coverage of such critical discoveries as stem cells, apoptosis, role of the amygdala in stress, and significance of dendritic spines. Among the core topics are pain and pain pathways, the visual system, development and growth of the nervous system, the chemical senses of smell and taste, and the limbic system. There is also fresh material on neuronal stem cells, the auditory and vestibular systems, neurotransmitters as the chemical messengers of certain brain circuits, the cerebellum, and lesions of the spinal nerves, spinal cord, and brainstem. The superb illustrations are designed to extract and visually highlight the essence of the neuroanatomical features embedded in the complexities of the nervous system.
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 | Z705756 | IGF and Nutrition in Health and Disease | A benchmark review on how specific nutrients or adverse nutritional states impact the components of the IGF (Insulin-like Growth Factor) system and affect human health and disease. The authors describe the fundamentals of the IGF system, explain its basic mechanisms of action, illuminate its essential role in physical development across the life span, and highlight the link between normal and abnormal cellular growth resulting in tumor formation. They also fully examine the clinical aspects of nutrition and IGF. Special attention is given to critical illness, diabetes, gastrointestinal diseases, kidney failure, endocrine dysfunctions, cardiovascular disease, neurological disorders, and cancer, as well as to their potential for IGF therapy.
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 | Z652008 | Imaging in Neuroscience and Development: A Laboratory Manual | As imaging studies have continued to expand in scope and sophistication, this edition has expanded to encompass applications in developmental biology. It has more than 20 new chapters on such topics as in vivo imaging in a variety of model systems, calcium imaging, imaging early developmental events, and labeling single neurons. Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes an additional forty shorter chapters of protocols for imaging techniques.
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 | Z651990 | Imaging in Neuroscience and Development: A Laboratory Manual | As imaging studies have continued to expand in scope and sophistication, this new edition of the highly successful and well-received Imaging Neurons: A Laboratory Manual has expanded to include Development, with over 20 new chapters on such topics as MRI microscopy, imaging early developmental events, and labeling single neurons.
Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes over forty sections of protocols for imaging techniques.
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 | I0147 | In Vitro Neurochemical Techniques | Techniques range from isolation of neuronal genes and their expression to analysis of receptor-ligand interactions, to characterization of consequences of receptor activation. Methods include electrophysiological techniques to explore functional properties of receptors present in membranes of excitable cells and those to isolate novel genes central to neurobiological processes. Others cover measuring changes in gene expression, rapid identification of gene polymorphisms, and identification and characterization of second messenger pathways.
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 | Z704431 | Interferon Methods and Protocols | A compendium of optimized methods to measure type I interferon efficacy as an antiproliferative or an antiviral agent. These cutting-edge techniques range from the simple to the highly complex and serve to illuminate the signaling cascades and the activation of enzymatic pathways prompted by interferon. Each protocol offers step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. This book offers researchers powerful tools not only to ascertain the functions of IFN-stimulatory gene products, but also to identify additional molecular pathways that will clarify our understanding of the many biological events influenced by IFNs.
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 | C1601 | Introduction to Cellular Signal Transduction | This book is designed for those interested in a basic understanding of the major participants in the cellular Signal Transduction pathways. The topics are covered with the goal of preparing the reader for a subsequent in-depth examination of specific areas of interest. The book covers most of the major topics in Signal Transduction: receptors, which recognize the signals at the cell surface; G-proteins which transduce the signals; and adenylate cyclases, guanlate cyclased, and phospholipases which generate second messengers. The discussion also focuses on ion channels, phosphodiesterases, protein kinases, phosphoprotein phosphatases and nitric oxide which participate in the cellular response to signals, the health consequences of defects in Signal Transduction proteins, and the central role Signal Transduction plays in drug abuse.
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| | Z730904 | Introduction to Nervous Systems Hardback | This book presents the principles of neurobiology from an evolutionary perspective-from single-celled organisms to complex invertebrates such as flies-and is ideal for use as a supplemental textbook. Greenspan describes the mechanisms that allow behavior to become ever more sophisticated-from simple avoidance behavior of Paramecium through to the complex cognitive behaviors of the honeybee-and shows how these mechanisms produce the increasing neural complexity found in these organisms. The book ends with a discussion of what is universal about nervous systems and what may be required, neurobiologically, to be human.
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 | Z730742 | Invertebrate Neurobiology | This monograph captures the current excitement in invertebrate neurobiology, from the manipulation of activity in specified neurons to the investigation of behavior. The monograph emphasizes the evolutionary aspects of neurobiology and work on non-model species. The chapters, from internationally respected contributors, incorporate trenchant findings but also identify important outstanding questions. The volume will inspire graduate students and post-docs, and provide a good starting point for any scientist who wants to learn about this resurgent field.
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 | I4029 | Ion Channels and Disease | A number of human and animal diseases have been found to result from mutations in the genes encoding ion channel proteins, and are referred to as channelopathies. This chronicles our understanding of the molecular basis of ion channel diseases. It includes the relevant aspects of molecular biology and biophysics and a description of methods used to study channelopathies. For each channel, the relationship between its molecular structure and its functional properties is discussed and ways in which genetic mutations produce the disease phenotype are considered.
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 | Z705519 | Ion Channels: Methods and Protocols | It is now clear that ion channels play essential roles in cell biology and physiology and that their dysfunction is the root cause of many human diseases. In this book internationally recognized biomedical scientists describe recent technological braekthroughs and demonstrate their use in successful experimental designs. The diverse applications range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterogenous expression of ion channels in cells for determining channel structure-function, for studying channel regulation and physiological function and for genetic screening and investigating channelopathies.
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 | Z701068 | Ionotropic Glutamate Receptors as Therapeutic Targets | This book provides an overview of therapeutic strategies utilizing modulators of ionotropic glutamate receptor function. Each chapter focuses on the role of glutamate in a particular disorder, and preclinical/clinical experience with agents targeting ionotropic glutamate receptors. The chapters cover: AIDS, Anxiety, Brain Trauma, Dementia, Depression, Drug Abuse, Drug Tolerance, Epilepsy, Glaucoma, Tinnitus, Pain, Schizophrenia, Neurodegenerative Disorders (ALS - Huntington’s Disease - Parkinson’s Disease), L-DOPA-induced Dyskinesia, Spinal Cord Trauma, Stroke. Symptomatological and neuroprotective effects have been discussed separately.
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 | K4637 | Kinesin Protocols | Kinesin-like proteins (KLP) are thought to be responsible for transport of cellular cargoes and involved in many cellular processes. This book presents reproducible techniques for identification, purification, and characterization of the kinesin superfamily of microtubule-dependent motors. Methods include step-by-step instructions and cautionary notes. Considered are methods to express and purify kinesins in different systems, to characterize microtubule-enhanced ATPase activity and motility properties, and to test microtubule destabilizing activity.
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 | Z731234 | Ligand Design for G Protein Coupled Receptors | G protein-coupled receptors (GPCRs) are one of the most important target classes in pharmacology and are the target of many blockbuster drugs. Yet only with the recent elucidation of the rhodopsin structure have these receptors become amenable to a rational drug design. Based on recent examples from academia and the pharmaceutical industry, this book demonstrates how to apply the whole range of bioinformatics, chemoinformatics and molecular modeling tools to the rational design of novel drugs targeting GPCRs.
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 | Z652016 | Live Cell Imaging: A Laboratory Manual | Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a CD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book.The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
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 | Z701947 | MAP Kinase Signaling Protocols | A collection of techniques for the detection and measurement of mitogen-activated protein kinase (MAPK) signaling cascades. Protocols include methods for the determination of the subcellular localization of these components, structural and biophysical analysis of the components, and identification of novel components of known and unknown signaling cascades. Additional methods examine the upstream mechanisms of activation of MAPK cascades by various receptors, mechanisms involved in the down regulation of the MAPK cascades, and identification of targets of the MAPK cascades. It offers all the techniques needed in to conduct productive studies of MAPK signaling in health and disease, in measuring the influence of drugs, and in a broad range of experimental systems.
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 | M6441 | Mass Spectrometry and Hyphenated Techniques in Neuropeptide Research | In analyzing the effects of experimental drugs on the brain, mass spectrometry is the sole technique for identifying the presence and structure of neuropeptides–substances that indicate the effect of the drug. This book explains how to apply the technology to this process. It is written by mass spectrometry users, not mass spectrometrists, so the focus remains on practical applications. Readers will learn why mass spectrometry provides more outcome features than other techniques in neuropeptide analysis, including simultaneous detection, identification of substances present in mixtures, and sequence information even when the residues are modified, blocked, or unusual.
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 | Z703613 | Membrane Microdomain Signaling: Lipid Rafts in Biology and Medicine | Cutting-edge articles review the current understanding of lipid microdomain signaling mechanisms and their physiological and pathological importance. The book describes the role of lipid rafts in learning, memory, and cancer, presents the emerging evidence that lipid rafts play critical roles in signaling pathways and the regulation of synaptic function in the nervous system, and shows how alterations in lipid raft metabolism are implicated in the pathogenesis of neurodegenerative disorders. Techniques are also described for the isolation of lipid rafts, the analysis of the lipid and protein components of lipid rafts, the imaging of lipid rafts in living cells, and the analysis of signal transduction in lipid rafts.
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 | Z701122 | Membrane Transporters: Methods and Protocols | This collection of methodologies for studying membrane transporters and channels takes advantage of all the developments in biomedical research, including pharmacogenomcs, bioinformatics, and microarray technologies. The authors explain databases and tools for bioinformatics studies, provide practical guidelines for microarray experiments and data analysis, and illustrate the use of small angle x-ray scattering, nuclear magnetic resonance (NMR), and molecular modeling to study the structural biology of membrane transporters. Methods for exploring structure-function correlation, such as site-directed mutagenesis, immunocytochemistry, and confocal microcopy are also described.
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 | Z700304 | Microarrays for the Neurosciences | This book provides a guide to the use of DNA microarrays in neuroscience. Human nervous system tissue is remarkably complex. The number of cell types, the architecture, the developmental program, and the importance of environmental factors in development and functioning all pose particular challenges to researchers using gene expression studies. After an overview of the technology, the book discusses array scanning and image application, statistical methods for array analysis, specific applications of gene expression studies in the central nervous system, the use of postmortem human tissue, and novel methods for using microarray data to develop hypotheses about regulatory networks.
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 | Z700274 | Molecular Biology of Drug Addiction | A review of the most relevant molecular, genetic, and behavioral approaches used to investigate the neurobiological basis of drug addiction. Latest findings on opioid, psychostimulant, cannabinoid, alcohol, and nicotine addiction are presented and provide insights into the genetic basis of drug addiction and new therapeutic perspectives these have opened. Authors describe the technology available to generate conditional knockout mice and show how these mice can reveal the molecular basis of opioid, psychostimulant, and cannabinoid addiction. They also review the different behavioral models available to evaluate the rewarding effects of drugs and analyze the genes involved in alcohol dependence.
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 | Z703842 | Molecular Biology of the Neuron, 2nd ed. | Nerve cells - neurons - are arguably the most complex of all cells. From the action of these cells come movement, thought and consciousness. It is a challenging task to understand what molecules direct the various diverse aspects of their function. In the six years since the first edition there has been an explosion in the molecular information about neurons that has been discovered, and this information is incorporated into this second edition. Entirely new chapters have been introduced where recent advances have made a new aspect of neuronal function more comprehensible at the molecular level. The book provides an essential overview of the molecular structure and function of neurons.
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 | Z700894 | Multiple Drug Resistant Bacteria | This book focuses on the problem posed by resistant organisms: multi-resistance. Resistance and multi-resistance arise as consequences of a complex set of biological, social and economic forces. Bacteria gain multi-resistance in a number of ways, gathering individual resistance genes in single genetic elements, activating responses to environmental stress, and growing in biofilms. Two of the most dangerous pathogenic organisms (methicillin-resistant S. aureus and vancomycin-resistant enterococci) pose a threat because they are always multi-resistant are covered. Chapter 7 is devoted to explain a different way to look into the evolution of resistance and a way to search for new antimicrobial drugs.
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| | Z375535 | Neuropeptide Protocols: Methods in Molecular Biology, Vol. 73 | | pricing |
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 | N2773 | Neurotransmitter Release | This book emphasizes the rationale by which proteins are assigned specific functions rather than just providing facts about function. Chapters cover properties of the synapse, techniques used to elucidate aspects of release, approaches used to identify proteins involved in neurotransmitter release, aspects of synaptic transmission, proteins that transport neurotransmitters and role of phospholipids in the process. Other topics include approaches to unravel the function of proteins in vivo along with endocytosis and recycling.
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 | N4410 | Neurotransmitter Transporters: Structure, Function and Regulation, 2nd ed. | This edition is updated to include progress in characterizing the structure, function, regulation, and physiological relevance of each transporter. It includes discussions of gene organization and the relationship of polymorphisms of monoamine transporters with a variety of diseases, transgenic animals carrying altered genes for plasma membrane monoamine transporters, and the regulation of transporters by trafficking in relation to their phosphorylation state. Also addressed are transporter imaging techniques that allow in vivo measurement in men and animals, chimera and site-directed mutagenesis of transporters, and transporter electrophysiology.
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 | Z701165 | Neurotransmitters and Neuromodulators: Handbook of Receptors and Biological Effects | What are the biological effects of neuropeptides and other neuroactive substances? What are their receptors, their pharmaceutical applications? This handbook gives a detailed overview of neuroactive substances, covering all neurotransmitters and most neuromodulators. It provides comprehensive information on their biosynthesis and degradation, localization, receptors, signal transduction pathways, and biological effects in the central nervous system. Chapters on general aspects and on methods for analyzing neuroactive substances make this a valuable tool.
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 | Z704229 | Nitric Oxide Donors: For Pharmaceutical and Biological Applications | Nitric oxide is a highly potent regulatory molecule with great pharmaceutical potential. This handbook fills a real gap in combining the chemistry of nitric oxide releasing substances with their practical applications in biology and drug design. It covers all classes of nitric oxide donors, from organic nitrates to nitroso compounds, guanidines and metal-NO complexes. In addition to a detailed treatment of the chemistry of NO donors, numerous examples of successful diagnostic and pharmacological applications are discussed, as well as further therapeutic targets for these substances.
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 | Z702722 | Nitric Oxide Protocols, 2nd ed. | A collection of cutting-edge techniques for measuring nitric oxide and the enzyme that produces it in biological tissues and fluids. These readily reproducible methods can be used to measure novel nitric oxide-related products such as protein nitration and nitrosation, as well as to express nitric oxide synthase in basic research and gene therapy using viral vectors. This book offers a wealth of important practical information that will be of inestimable value both to newcomers and experienced research groups along with being an essential resource for every laboratory involved in NO-related work
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 | Z374148 | Nitric Oxide Synthase: Characterization and Functional Analysis | This book contains comprehensive protocols for the detection of nitric oxide (NO) and related compounds by chemical, immunohistochemical, and in situ hybridization techniques. Newly developed methods for the purification of neuronal and endothelial NO synthase, and molecular cloning and expression of NO synthase are included.
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| | Z374156 | Nitric Oxide: Principles and Actions | | pricing |
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 | Z340812 | Nuclear Receptors: A Practical Approach | The steroid or nuclear receptor superfamily is an important group of transcription factors of growing importance to a large number of researchers. Topics include how to clone, characterize, identify, assay for, monitor changes, and analyze kinetics of novel receptors along with characterization of co-activators, receptor interactions, ligand binding studies, DNA binding studies, and assays such as CARLA.
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 | Z700193 | Nuclear Transport | Bidirectional traffic of macromolecules across the nuclear envelope is an active and essential transport process in all eukaryotic cells. Work on various model systems has led to an increase in our understanding of nuclear transport in recent years. This volume brings together our current knowledge of protein and RNA transport into and out of the nucleus. It contains nine up-to-date reviews which cover various aspects of nucleocytoplasmic transport including the structure and function of the nuclear pore complex, the role of soluble transport factors in protein or RNA transport, and the regulation of protein transport through the nuclear pore.
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 | O1264 | Oxidative Stress Biomarkers and Antioxidant Protocols | This is a collection of methods for evaluating perturbations in cell function resulting from increased oxidative stress. These techniques cover free radical-derived and antioxidant biomarkers. Methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, GC-MS, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation.
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 | Z700657 | Patch-Clamp Analysis: Advanced Techniques | This book describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, it presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.
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 | P2988 | Pharmacology of Cytokines | Modulation of production and action of cytokines has been the object of intense research. This book overviews the field of cytokine research and describes the various approaches that have been taken to develop the pharmacology of these novel mediators. The book in the framework of the immunobiology of cytokines, examines interactions with the cytokine system of a variety of compounds ranging from simple synthetic chemicals to biotechnological products. It analyzes specific contexts for the pathophysiology of these mediators as well as pharmacological approaches for their control.
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 | Z704423 | Phosphodiesterase Methods and Protocols | Research leaders describe new concepts and techniques for investigating the role of PDEs in orchestrating normal and pathophysiological responses. These methods allow the measurement of cyclic nucleotide variations in living cells, as well as their visualization in a spatio-temporal manner, the localization and characterization of their activities in tissues and living cells, and the assessment of targeted PDEs in creating specific tools and drugs. Additional chapters discuss the generation of PDE4 knockout mice to demonstrate not only the potential role of targeted PDEs, but also their use in further studies of the central role of PDE regulation in intracellular signaling control. Alterations of intracellular signaling related to PDE deregulation in such pathologies as inflammation, neurodegeneration, and cancer may explain the difficulties observed in their prevention and treatment.
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 | P1603 | Phospholipid Signaling Protocols | This collection of state-of-the-art techniques for the assay of phospholipid and phospholid-derived second messengers allows the identification and quantitation of signal pathway activation. Techniques cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase activities and for the separation and identification of phospholipids, diacylglycerol and sphingolipids, as well as their phosphoinositol, choline and fatty acid metabolites.
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 | P5736 | Principles Of Molecular Recognition | Conn and Means provide an integrated review of the key areas—membrane receptor-initiated cell signaling and nuclear-receptor initiated gene regulation. These authors illuminate the molecular machinery underlying the regulatory processes of cells. In addition to their review of the signaling mechanisms involving cell surface receptors, nuclear receptors, and ion channels, they detail the roles of calcium, lipids, cyclic AMP, protein kinases, and protein phosphatases.
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 | Z702285 | Programmed Cell Death in Plants | This single volume draws together new information from researchers on this emerging topic. The book refers to relevant PCD paradigms in other organisms, describes the cell death events in reproductive tissues, and discusses PCD events in vegetative tissues and the evidence that links PCD regulation to cell cycle regulation. It also investigates the important role of PCD in plant responses to biotic stress. The author distills the difficult literature and summarizes the various signaling pathways involved, clarifying a subject critical to plant breeding. This volume concludes by stating how new model systems, careful assessment of cell death events, and markers specific to plant PCD are each required for additional insight into plant PCD processes
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 | Z340855 | Protein Phosphorylation: A Practical Approach, 2nd Edition | This new edition provides a description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells, analysis of signal transduction pathways, kinase specificity and kinase interactions, assay and purification of kinases and phosphatases, and identification of substrates.
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 | Z705349 | Quest for Consciousness: A Neurobiological Approach | Consciousness is the major unsolved problem in biology. How do the elemental feelings and sensations making up conscious experience, the redness of red and the painfulness of pain, arise from the concerted actions of nerve cells and their associated synaptic and molecular processes? Can such feelings be explained by modern science, or is some different kind of explanation needed? And how can this seemingly intractable problem be approached experimentally? Designed as an introduction to the field and drawing upon anatomical, physiological, clinical and psychological observations, this book seeks answers to these questions with a neurobioloigcal framework; that is, how do the operations of the conscious mind emerge out of the specific interactions of myriads of neurons?
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 | Z704369 | Receptor Binding Techniques, 2nd ed. | A collection of methods for studying receptors in silico, in vitro, and in vivo. These techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knockout mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan PCR. This book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging
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 | Z379816 | Receptor Localization: Laboratory Methods and Procedures | A user-friendly guide to the latest techniques for localizing neurotransmitter receptors, in both the central and peripheral systems. It covers generalized procedures like genes and ligands and in vitro receptors. Additional focus is given to specialized applications like receptor sub-types. Standard laboratory methods and “tricks of the trade” are discussed by leading experts, and are fully illustrated.
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 | Z702536 | Receptor Signal Transduction Protocols, 2nd ed. | This edition contains new key methodologies that have emerged recently for studying G protein-coupled receptors (GPCRs). The methods are focused primarily on events at the receptor level, including ligand binding, the genetic manipulation of receptors, the generation of model cell lines in which to study them, and the interaction and activation of G-proteins. Additional methods concentrate on receptor expression and localization, receptor internalization and post-translational modification, GPCR protein interactions, and the use of knock-out and knock-in strategies for determining the physiological roles of receptors.
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 | Z700983 | Receptors: Structure and Function 2nd ed., A Practical Approach | Given the number of exciting developments across the spectrum of receptor research in recent years, the editors have not restricted themselves to one particular approach or class of receptors. Thus they include studies from G protein-coupled surface receptors to the delivery of antisense DNA inside living cells systems. This is a review of new methods for studying cellular receptors and examines how each technique can be used on different receptor types.
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 | R0402 | Receptors: Structure and Function, A Practical Approach | Given the number of exciting developments across the whole spectrum of receptor research in recent years, the editors have not restricted themselves to one particular approach or class of receptors. Thus the studies within range from G protein-coupled surface receptors to the delivery of antisense DNA inside living cells systems. This is a comprehesive review of new methods that can be used for studying cellular receptors and examines how each technique can be used on different receptor types.
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 | Z701726 | Short Protocols in Cell Biology | Providing condensed descriptions of more than 500 methods compiled from "Current Protocols in Cell Biology", this text explores cell biology in an easily accessible, hands-on format. This is an authoritative and indispensable guide for all life scientists and researchers. The text is designed to provide quick access to step-by-step instructions for the essential methods used in every major area of cell biological research, and it contains methods from every aspect of cell biology everything needed to study the basic structure and functions of cells at both the molecular and cellular levels
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 | Z705985 | Short Protocols in Neuroscience: Cellular and Molecular Methods | This volume is an easy-to-use compendium of cellular and molecular methods from Current Protocols in Neuroscience, part of the acclaimed series that has set the standard of excellence for laboratory manuals. It provides a portable and streamlined at-the-bench resource, covering methods for analysis of neural cell strucutre and function, isolation and culture of neural cells and characterization of neural cell gene expression.
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 | Z705993 | Short Protocols in Neuroscience: Systems and Behavioral Methods | Short Protocols in Neuroscience: Systems and Behavioral Methods provides a portable and streamlined at-the-bench resource of systems and behavorial methods from the acclaimed Current Protocols in Neuroscience. It covers areas such as electrophysiological analysis of neural cells, the chemistry and pharmacology of the nervous system, behavioral analysis, and animal models of neurologic and psychiatric disorders.
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 | S8188 | Sigma-RBI Handbook of Receptor Classification and Signal Transduction | Over 100 research scientists collaborated with Sigma-RBI to compile new and up-to-date information on receptors and enzymes involved in cell signaling mechanisms. Information is detailed in over 90 separate entries covering a range of receptors, ion channels and key cell signaling enzymes. New entries include Adenylyl Cyclases, GABA Transporters, Glycine Transporters, Lysophosphatidic Acid (EDG) Receptors, Neurotrophin Receptors, Orexin Receptors, Peroxisome Proliferator Activated Receptors and Small Molecular Weight G Proteins. An overview is provided for each entry with a list of 12 key references and a chart summarizing the classification of the subject receptors, ion channels or enzymes and detailing key ligands.
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 | Z705403 | Sigma-RBI Handbook of Receptor Classification and Signal Transduction, 5th ed. | The expanded 5th edition presents concise information on the characterization and classification of a wide range of receptors, ion channels, transporters, neurotransmitter synthesis/metabolism pathways and intracellular signaling enzymes. In addition to updating and revising over 80 previous handbook entries, 26 new entries have been added with a strong focus on kinases and ion channels. Each peer-reviewed entry consists of an easy-to-read and up-to-date overview of the available information in the area, as well as a list of 12 key references to guide both the new investigator and the experienced researcher in their searches and reviews of the current literature. Each entry includes a table that presents the currently accepted classification of a given receptor, ion channel, enzyme or transporter into its component subtypes and subgroups. It also highlights the pharmacological tools with which to investigate their function and regulation.
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 | S2066 | Signal Transduction | This text explains how cells respond to external cues (hormones, cytokines, neurotransmitters, adhesion molecules, extracellular matrix etc), and shows how these inputs are integrated and coordinated. The first half explains the formation and action of second messengers, particularly cyclic nucleotides and calcium, and the mediation of signal pathways by GTP-binding proteins. Later it deals with the formation of complex signaling cascades employed by cytokines and adhesion molecules, starting at the membrane and ending in the nucleus, to regulate gene transcription. It ends with a description at the molecular level of how signaling proteins interact with their environment and with each other through their structural domains.
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 | Z702897 | Signal Transduction Protocols 2nd ed. | Carrying on the high standards of the acclaimed first edition, highly experienced investigators have extensively updated the first edition with many of the new approaches that have been transforming the field. Included in this edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class.
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| | Z364177 | Signal Transduction Protocols: Methods in Molecular Biology, Vol. 41 | | pricing |
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 | Z701084 | Signal Transduction and Human Disease | This book uniquely relates the broad impact of signal transduction research on the understanding and treatment of human disease. There have been significant advances in the area of signaling in disease processes, yet no resource presently connects these advances with understanding of disease processes and applications for novel therapeutics. Given the emphasis on translational research and biological relevance in biotechnology, and, conversely, the importance of molecular approaches for clinical research, it is evident that a single resource bridging signaling research and human disease will be invaluable
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 | Z702757 | Signal Transduction by Reactive Oxygen and Nitrogen Species: Pathways and Chemical Principles | Reactive oxygen and nitrogen species (ROS/RNS) have taken center stage in the field of signal transduction. The enzymes responsible for the production of ROS and RNS have been unraveled and the participation of these species in numerous signaling pathways has been documented. The next step is to identify the targets of ROS and RNS and the mechanisms by which they alter their activity in the affected signaling pathway. . This book provides relevant chemistry that can be applied across signaling systems and summarizes the current state of knowledge in the area of redox signaling. ROS and RNS have been implicated in inflammation, aging and cancer.
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 | Z718505 | Signaling & Gene Expression in the Immune System | For the past two decades, much effort has been made to understand the complex interactions between lymphocytes and antigen-presenting cells that result in immune responses. Immunologists have identified an extraordinary diversity of cytokines as players in these interactions, some the products of cellular activation which themselves activate surrounding cells. Meanwhile, another community of investigators, interested in how genes turn on, were using lymphocytes as easily cultivated cells with which to study transcriptional regulation from a molecular perspective. Connections between these areas of study, with their quite different technical vocabularies and experimental approaches, were limited. The 64th Annual Cold Spring Harbor Symposium was therefore a particularly imaginative meeting, intended to provoke a dialogue on the nature of the membrane signals and intracytoplasmic events that provoke the generation of immunity. It did. The debate ranged over biochemistry, physiology, and molecular genetics as well as classical cellular immunology, involving over 70 of the world's leading investigators in a discussion described by the summarizer as the best he had ever attended. The book prompted by the meeting contains personal summaries of the contributions these investigators have made to their fields over the years, with reference to their most recently reported data. This volume therefore contains a remarkably fresh and original perspective on an important aspect of immunology that is necessary to understand in order to undertake rational manipulation of immune responses. All laboratories committed to the future of immunology should have this book, as well as investigators in gene regulation seeking a wide-ranging survey of results achieved with lymphocytes.
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 | Z718513 | Signaling & Gene Expression in the Immune System | For the past two decades, much effort has been made to understand the complex interactions between lymphocytes and antigen-presenting cells that result in immune responses. Immunologists have identified an extraordinary diversity of cytokines as players in these interactions, some the products of cellular activation which themselves activate surrounding cells. Meanwhile, another community of investigators, interested in how genes turn on, were using lymphocytes as easily cultivated cells with which to study transcriptional regulation from a molecular perspective. Connections between these areas of study, with their quite different technical vocabularies and experimental approaches, were limited. The 64th Annual Cold Spring Harbor Symposium was therefore a particularly imaginative meeting, intended to provoke a dialogue on the nature of the membrane signals and intracytoplasmic events that provoke the generation of immunity. It did. The debate ranged over biochemistry, physiology, and molecular genetics as well as classical cellular immunology, involving over 70 of the world's leading investigators in a discussion described by the summarizer as the best he had ever attended. The book prompted by the meeting contains personal summaries of the contributions these investigators have made to their fields over the years, with reference to their most recently reported data. This volume therefore contains a remarkably fresh and original perspective on an important aspect of immunology that is necessary to understand in order to undertake rational manipulation of immune responses. All laboratories committed to the future of immunology should have this book, as well as investigators in gene regulation seeking a wide-ranging survey of results achieved with lymphocytes.
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 | S8563 | Signaling Networks and Cell Cycle Control | Leading scientists summarize latest findings on signal transduction and cell cycle regulation and describe the effort to design and synthesize inhibiting molecules, as well as to evaluate their biochemical and biological activities. They review relevant cell surface receptors, their ligands, and their downstream pathways. Also examined are the latest findings on components of novel signaling networks controlling activity of nuclear transcription factors and cell cycle regulatory molecules. This is a guide for all investigators of cell regulation and its emerging pharmacological opportunities.
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 | S9932 | Signaling Through Cell Adhesion Molecules | The field of signal transduction research is one of the fastest growing in all of biomedical research in recent years. Signaling through cell adhesion molecules have long been of interest because of their importance in embryonic development, homeostasis, immune responses, wound healing, and malignant transformation. However, it is only recently realized that cell adhesion molecules are capable of transducing biochemical signals across the plasma membrane to regulate cellular functions. Many chapters not only contain the ‘recipes‘ on experimental protocols, but also discuss the rationale behind their development.
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 | Z378119 | Signalling by Inositides: A Practical Approach | Topics covered include: measurement of inositol phosphate turnover in intact cells and cell-free systems, purification and assay of inositide binding proteins, desalting inositol polyphosphates by dialysis, and the assay of phosphoinositide 3-kinase. Each chapter contains detailed step-by-step protocols, typical results and key citations to literature. An appendix lists synthetic inositide derivatives which are not currently available commercially, and the research laboratories where they have been prepared.
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| | S9685 | Structure-Function Analysis of G Protein-Coupled Receptors | G Protein Coupled Receptors (GPCR) often play key roles in a large number of physiological and pathophysiological conditions. Theses receptors are the targets of a large number of therapeutic drugs. Detailed knowledge about the molecular structure of GPCRs should pave the way for more efficient drugs. This lab-friendly guide provides a concise, up-to-date presentation of methods which have been used successfully in studying structure and function of GPCRs.
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 | Z701971 | Textbook of Receptor Pharmacology, 2nd ed. | In the bestselling first edition the innovative format united four major approaches to the study of receptors: molecular biology, quantitative functional studies of agonists and antagonists, ligand binding, and signal transduction systems. The second edition builds on this foundation. It streamlines the material and focuses on cell membrane receptors along with their immediate signal transducers. The section on the molecular structure of receptors reflects the advances in this area. This edition also includes two restructured new chapters, one on G-proteins and one on tyrosine kinases, as signal transductors.
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 | Z705616 | Transmembrane Signaling Protocols 2nd ed. | This fully updated and expanded second edition of Transmembrane Signaling Protocols demonstrates the use of such techniques as single cell imaging, bioluminecence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein–protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo.
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 | Z379719 | Transmembrane Signaling Protocols: Methods in Molecular Biology, Vol. 84 | | pricing |
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 | A8968 | Using Antibodies: A Lab Manual | Harlow and Lane have revised their guide to the use of immunoglobulin reagents in the laboratory. Chapters have been rewritten, reorganized, and updated to provide background, context and step-by-step instructions. Techniques range from choosing the right antibody and handling it correctly to the proper methods for characterizing antigens in cells and solutions. New chapters on tagging proteins and epitope mapping are included. Rather than presenting an array of solutions for working with antibodies and antigens, this title instead identifies in each case the best approach to specific problems.
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 | Z705551 | Voltage Gated Ion Channels as Drug Targets | Edited by the most prominent person in the field and top researchers at US pharmaceutical companies, this is a unique resource for drug developers and physiologists seeking a molecular-level understanding of ion channel pharmacology. After an introduction to the topic, the authors evaluate the structure and function of ion channels, as well as related drug interaction. A section on assay technologies is followed by a section each on calcium, sodium and potassium channels. Further chapters cover genetic and acquired channelopathies, before the book closes with a look at safety issues in ion channel drug development.
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 | Z730521 | What’s Wrong With My Mouse Behavioral Phenotyping of Transgenic and Knockout Mice, 2nd ed. | Turn to What’s Wrong with My Mouse? to discover the wealth of mouse behavioral tasks and to get the guidance you need to select the best methods and necessary controls. Chapters are organized by behavioral domain, including measurements of general health, motor functions, sensory abilities, learning and memory, feeding and drinking, reproductive, social, emotional, and reward behaviors in mutant mice. Throughout the chapters, new behavioral tasks and new research discoveries have been added, bringing the Second Edition up to date with the latest science. In addition, it includes two new chapters: "Neurodevelopment and Neurodegeneration" discusses mouse behavioral tasks relevant to neurodevelopmental diseases, such as mental retardation and autism, and to neurodegenerative diseases, such as Alzheimers, Parkinsons, Huntingtons, and amyotrophic lateral sclerosis. "Putting It All Together" recommends strategies for optimizing a battery of behavioral phenotyping tests to address your specific hypotheses about gene functions.
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 | W2390 | What’s Wrong with My Mouse Behavioral Phenotyping of Transgenic and Knockout Mice | This concise review explains the differences between transgenic and knockout mice and their wild-type controls, while providing critical information about gene function and expression. Newly identified genes can provide insights into brain malfunctioning in disease states. It discusses how to generate a transgenic or knockout mouse, functions (open field, holeboard, rotarod, balance, grip, circadian activity, etc.), sensory abilities (olfaction, vision, hearing, taste, touch, nociception), reproductive behavior, social behavior, and emotional behavior.
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 | Z701742 | When Cells Die II: A Comprehensive Evaluation of Apoptosis and Programmed Cell Death | This edition offers the most thorough coverage of this field. Leading researchers present an up-to-date survey ranging from the history of cell death science to its modern methodology. Revised to include major advances in research, this edition features relevant discussion of: The impact of genomics and proteomics; Gene therapy and pharmacogenetics; Role of mitochondria; Caspase-independent and non-apoptotic cell death; Evolution of mechanism. With the manipulation of programmed cell death in clinical situations, this book also addresses the role of apoptosis in specific organ systems; the immune system, nervous system, and gastrointestinal tract; as well as different disease states, including viral infection, cancer, and myocardial infarct.
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 | Z705128 | Xenopus Protocols: Cell Biology and Signal Transduction | A collection of standard and cutting-edge techniques for using Xenopus oocytes and oocytes/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance, its striking protein translation capacity, and its breathtaking possibilities for the assembly of infectious viral particles by single cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction, and cover all the major uses of oocytes/extracts as experimental models.
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