This volume focuses on the polytene chromosome in three parts: morphology and structure, organization and function, and transcription patterns and replication. Includes more than 100 figures and 2900 references.
All About Albumin: Biochemistry, Genetics, and Medical Applications
Provides complete coverage of all aspects of serum albumin in one volume. It summarizes the chemistry, genetics, metabolism, clinical implications, and commercial aspects of this essential protein. Additionally, up-to-date sequences, structures, and compositions of many species can be found.
Use of antisense oligonucleotides is a powerful technique for silencing specific genes, but it requires careful optimization and species-specific methods for success. Each chapter is written by an acknowledged expert with a record of success at the bench; each includes authoritative guidance, tips for success, and trouble-shooting advice.
An authoritative survey of this fascinating strategy for drug discovery: using complementary oligonucleotides to block expression of disease-associated genes. Contributors discuss selection of gene targets, synthesis of oligonucleotides, routes of administration, and issues of pharmacokinetics. Current research on anti-viral drugs, cancer treatment, and therapies for genetic disorders is summarized.
Recent advances in genetics, synergized by many exciting developments in cellular and molecular biology, have provided powerful new experimental tools for the study of not only B-lymphocyte development and function, but also the physiology and pathology of the immune system in higher animals. This book is a diverse collection of protocols ranging from the latest inventions and applications to some classic, but still frequently used, methods in B cell biology. These readily reproducible protocols follow the successful Methods in Molecular Biology series format.
A collection of key cytogenetic and FISH techniques used by modern clinical laboratories in the genetic analysis of human malignancies. The book's practical advice and methods are suitable for use at every level of expertise, including fully established laboratories, but with a sympathetic bias towards anyone considering setting up a new cytogenetics service. Here the reader will find not only elementary tutorials on the fundamentals of human karyotypes and chromosome analysis, but also detailed discussions on how laboratories may optimally upgrade their repertoire of capabilities to include such newer complementary techniques as CGH, FISH, and M-FISH.
Cells of the Body: A History of Somatic Cell Genetics
This book is the first scholarly history of research into the genetics of body cells, from its origins in the 19th century to the present day. Henry Harris, a well-known writer and a distinguished investigator in cell biology and cancer genetics, brings an unusually informed perspective to the technical aspects of his subject. He has written a book to be enjoyed not just by professional historians of science, but by working scientists in genetics, cell biology, and cancer research, from the graduate student level upwards. Its readers will derive a richer understanding of how and why the cells of the body are studied in the way that they are today.
The sixth edition has a broader coverage than its name implies, since it includes definitions of strictly genetic words along with a variety of non-genetic terms often encountered in the literature of genetics. There are about 7,000 definitions, and tables or drawings that illustrate 395 of these. In addition to the main body of the dictionary, this work features new Appendices covering the genomic sizes and gene numbers of about 30 organisms ranging from the smallest known virus to humans, an up-to-date listing of internet addresses for easy access to genetic databanks, and a list of developments, inventions and advances in genetics, cytology, and evolutionary science from the past 400 years.
This workbook is a companion to Discovering Molecular Genetics: A Case Study Course with Problems and Scenarios. One of the unique features of the latter is: Over 140 problems, including scenarios from history, mythology, movies, and TV series, which test students' abilities to apply molecular genetic concepts. Solutions and strategies for these problems are provided in the companion workbook, which is also available separately.
This book has been the world's most trusted genetics text for more than 30 years. With balanced, integrated reporting on both the basic science and clinical implications of genetics, the content has been thoroughly updated for this edition to keep abreast of this fast-changing subject. There is new coverage of hot topics such as gene therapy, the human genome project and ethical issues in medical genetics, while a brand new illustration program has been specially designed to further help students to understand concepts and retain information.
The regulation of gene expression in many biological processes involves epigenetic mechanisms. In this new volume, 24 chapters written by experts in the field discuss epigenetic effects from many perspectives. There are chapters on the basic molecular mechanisms underpinning epigenetic regulation, discussion of cellular processes that rely on this kind of regulation, and surveys of organisms in which it has been most studied. Thus, there are chapters on histone and DNA methylation, siRNAs and gene silencing; X-chromosome inactivation, dosage compensation and imprinting; and discussion of epigenetics in microbes, plants, insects, and mammals. The last part of the book looks at how epigenetic mechanisms act in cell division and differentiation, and how errors in these pathways contribute to cancer and other human diseases. Also discussed are consequences of epigenetics in attempts to clone animals..
Epigenetics: Symposia on Quantitative Biology, volume LXIX 2004 Hard cover
The importance of epigenetics regulation of gene expression, particularly in higher organisms is now clear and the 2004 Cold Spring Harbor Annual Symposium provided many new examples as well as insights into underlying mechanisms. The resulting volume - with over 60 papers from experts across the field - covers various aspects of nuclear organization and dynamics; genomic imprinting, chromosomal inactivation, and other examples of gene silencing; the histone and DNA modifications associated with these conditions; and the roles of RNA and protein regulators in establishing and maintaining these states.
Epigenetics: Symposia on Quantitative Biology, volume LXIX 2004 Soft cover
The importance of epigenetics regulation of gene expression, particularly in higher organisms is now clear and the 2004 Cold Spring Harbor Annual Symposium provided many new examples as well as insights into underlying mechanisms. The resulting volume - with over 60 papers from experts across the field - covers various aspects of nuclear organization and dynamics; genomic imprinting, chromosomal inactivation, and other examples of gene silencing; the histone and DNA modifications associated with these conditions; and the roles of RNA and protein regulators in establishing and maintaining these states.
Evolution is a new book on evolutionary biology that integrates molecular biology, genomics, and human genetics with traditional studies of evolutionary processes. • Recommended as a primary textbook for undergraduate courses in evolution • Required reading for biologists seeking a clear, current, and comprehensive account of evolutionary theory and mechanisms • Written by experts in population genetics, bacterial genomics, paleontology, human genetics, and developmental biology • Integrates molecular and evolutionary biology in ways that reflect current directions in research
Expression Genetics: Accelerated and High-Throughput Methods
The methods included here, i.e., mammalian expression vectors, would come into play once regulation of a gene has been discovered by differential display. Advances in alternatives to differential display are also presented, including AFLP, arrays, protein display, and the two-hybrid system. These 37 papers selected from the journal BioTechniques along with their accompanying updates emphasize technologies that accelerate screening and selection assays.
This volume includes 60 key papers covering differential display and related techniques selected from the pages of BioTechniques by two pioneers of this important expression methodology. A special feature of this book is the introduction, an original review chapter on contemporary differential display methods and applications written by the volume editors Dr. Arthur Pardee, who co-discovered the DD method, and Dr. Michael McClelland, co-discoverer of the closely related AP-PCR technique.
Fly Pushing: The Theory and Practice of Drosophila Genetics, 2nd Edition
A new edition of the classic handbook that has become a standard in the Drosophila field. The book is expanded to include topics in which classical genetic strategies have been augmented with new molecular tools. Included are such new techniques as homologous recombination, RNAi, new mapping techniques, and new mosaic marking techniques.
Functional Analysis of Bacterial Genes: A Practical Manual
This manual summarizes the principles and key methods used to analyze the function of genes in bacteria. It provides a guide to many approaches for the systematic analysis of gene function. Features include in-depth discussion of the theory and application of the methods, step-by-step practical guide to each method, experimental examples to indicate expected results, and contact information for suppliers and resource centers. This volume will interest all those who face the task of uncovering functions of uncharacterized genes emerging from large-scale sequencing.
Highly skilled investigators in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for the analysis of the mouse phenotype. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. State-of-the-art and highly practical, it constitutes an invaluable source of readily reproducible techniques for genetic researchers today.
Two decades ago Benjamin Lewin's Genes revolutionized the teaching of molecular biology and molecular genetics by introducing a unified approach to bacteria and higher organisms. Genes has remained at the cutting edge of molecular biology, covering gene structure, organization, and expression. Originally the text opened with the genetic code and worked toward genome structure. Genes VIII changed the approach to begin with the sequence of the human and other genomes and starts with complete coverage of recent advances in genomics. The coverage of genomics is then integrated throughout the text.
Genetic Analysis of Pathogenic Bacteria: A��Laboratory Manual
Five years' progressive refinement in the Cold Spring Harbor course in Advanced Bacterial genetics has produced a manual that teaches theoretical and practical molecular genetic approaches to bacterial pathogenicity. Chapters on concepts, technologies and applications are followed by 15 multifaceted experiments.
This volume is designed to provide an easily accessible introduction to core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies and specialized expression cloning and library screening strategies.
Genetic Switch, Third Edition, Phage Lambda Revisited
The first edition of Mark Ptashne’s 1986 book describing the principles of gene regulation in phage lambda became a classic in both content and form, setting a standard of clarity and precise prose that has rarely been bettered. This newly updated third edition focuses once again solely on phage, incorporating the most recent insights into gene expression in prokaryotes while retaining all the special qualities of the original edition.
Genetic Variation: A Laboratory Manual (pb) Paperback
This manual is the first compendium of protocols specifically geared towards genetic variation studies, and includes thorough discussions on their applications for human and model organism studies. It covers the complete spectrum of genetic variation-from SNPs and microsatellites to more complex DNA alterations, including copy number variation. The early sections of the manual are devoted to study design and generating genotype data, the use of resources such as HapMap and dbSNP, as well as experimental, statistical, and bioinformatic approaches for analyzing the data. The final sections include descriptions of genetic variation in model organisms and discussions of recent insights into human genetic ancestry, forensics, and human variation.
This manual is the first compendium of protocols specifically geared towards genetic variation studies, and includes thorough discussions on their applications for human and model organism studies. It covers the complete spectrum of genetic variation-from SNPs and microsatellites to more complex DNA alterations, including copy number variation. The early sections of the manual are devoted to study design and generating genotype data, the use of resources such as HapMap and dbSNP, as well as experimental, statistical, and bioinformatic approaches for analyzing the data. The final sections include descriptions of genetic variation in model organisms and discussions of recent insights into human genetic ancestry, forensics, and human variation.
Reveals the connections between genetics and specific diseases. Understand the science and the ethics behind genetics. Want to know more about genetics? This non-intimidating guide gets you up to speed on all the fundamentals. From dominant and recessive inherited traits to the DNA double-helix, you get clear explanations in easy-to-understand terms. Plus, you'll see how people are applying genetic science to fight disease, develop new products, solve crimes, and even clone cats. Discover: What geneticists do; How traits are passed on; How genetic counseling works; The basics of cloning; The role of DNA in forensics; The scoop on the Human Genome Project
Genetics: Analysis of Genes and Genomes represents the most current, comprehensive, and progressive introduction to genetics and genomics. Keeping pace with the latest developments in genetics, the authors treat classical, molecular, and population genetics as distinct but unified subjects that illuminate and reinforce each other throughout the text. This integrated approach to teaching genetics is a logical progression in an era when the various subdisciplines of genetics are so closely interwoven.
Guide to Yeast Genetics and Molecular and Cell Biology, Part B, Methods in Enzymology Vol 350
This volume and its companion, Volume 351, are specifically designed to meet the needs of graduate students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable anyone to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include basic techniques, making mutants, genomics, and proteomics.
Guide to Yeast Genetics and Molecular and Cell Biology, Part C, Methods in Enzymology Vol 351
This volume and its companion, Volume 350, are specifically designed to meet the needs of researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable anyone to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include cytology, biochemistry, cell fractionation, and cell biology.
In the small Fly Room at Columbia University, T.H. Morgan and his students, A.H. Sturtevant, C.B. Bridges, and H.J. Muller, carried out the work that laid the foundations of modern, chromosomal genetics. The excitement of those times, when the whole field of genetics was being created, is captured in this book, written in 1965 by one of those present at the beginning. His account is one of the few authoritative, analytic works on the early history of genetics. This attractive reprint is accompanied by a website offering full-text versions of the key papers discussed in the book, including the world’s first genetic map.
Human Cytogenetics: Constitutional Analysis, A Practical Approach, 3rd ed.
This covers all basic aspects of human cytogenetic study other than malignancies and abnormalities. Since the 2nd edition, the largest new development has been the transition of fluorescence in situ hybridization to an essential tool for cytogenetecists. Its use in chromosome analysis is covered in detail. Another new technology described in detail is computerised image analysis. Conventional techniques have not been forgotten, with chapters on chromosome staining and banding techniques and meiotic studies. New authors take a fresh look at lymphocyte culture and prenatal diagnosis
Human Cytogenetics: Malignancy and acquired abnormalities, A Practical Approach, 3rd ed.
The advent of molecular technologies has lead to a rapid acceleration in the cytogenetic study of malignancy and acquired abnormalities. As well as having a chapter devoted to molecular technologies (FISH, PRINS, and CGH), molecular methodology is emphasized in all chapters. There are three chapters on the specific methodology for the cytogenetic study of myeloid leukemia, acute lymphoblastic leukemia, and lymphomas and lymphoproliferative disorders. Also covered are chromosome instability syndromes, mutagen-induced chromosome damage in lymphocytes and the role of cytogenetics in the assessment of hematological disorders.
Human Genetic Disease Analysis: A��Practical Approach, 2nd ed.
This new edition covers all the important methods now commonly in use, including synthetic oligonucleotide hybridization, pulsed-field gel electrophoresis, and fluorescence in situ hybridization. This book describes not only the analysis of gene mutations but also the functional organization of genes and chromosome regions.
Each chapter focuses on a core concept of human genetics, illustrated by a corresponding clinical case that guides the reader through key principles. Topics from Mendelian, molecular, and quantitative genetics are covered in such a way that the role of genes in disease is easily understood.
This book provides a complete account of human molecular genetics in the post-genome era, from basic principles to current practice. It is designed to engage readers with detailed full-color illustrations, special topic boxes (coverage of ethical, legal and social issues) and a constant emphasis on understanding principles rather than memorizing detail. Initial chapters cover DNA structure and function, chromosomes, pedigrees and the basic techniques of PCR, cloning and hybridization, and include a new chapter on cells and development. Later chapters detail new understanding of the structure, organization, expression and evolution of the human genome. Final sections cover functional genomics, the role of animal models and new approaches to genetic treatment of disease.
Extensively rewritten and updated, this edition guides researchers through the very latest developments in this area. The highly regarded structure of the bestselling first edition is retained, but a wealth of new data and features have been added. It includes new material on cell types and the cell cycle, signal transduction, DNA mutation repair, and comparative genomics and evolution, new material on recent advances in the study of gene expression and function, including the use of DNA microarrays, and the latest Genome Project data including an assessment of the impact of complete genome sequences and new approaches in functional genomics
This two-volume set, “the handbook for the genome revolution,” is an essential guide to the enormous range of techniques for genetic and physical genome mapping, as well as sequencing and analysis of DNA. It is both a protocol manual and a comprehensive reference. The primary focus is on human genetics, but the book also considers other model organisms − bacterial, plant and animal. Exhaustive appendices include Internet resources, basic data on human and other genomes, and mouse gene knock-outs.
This latest book by Elof Carlson (The Unfit)is a first history of classical genetics, the era in which the chromosome theory of heredity was proposed and developed. Highly illustrated and based heavily on early 20th century original sources, the book traces the roots of genetics in breeding analysis and studies of cytology, evolution, and reproductive biology that began in Europe but were synthesized in the United States through new Ph.D. programs and expanded academic funding. Carlson argues that, influenced largely by new technologies and instrumentation, the life sciences progressed though incremental change rather than paradigm shifts, and he describes how molecular biology emerged from the key ideas and model systems of classical genetics. Readable and original, this narrative will interest historians and science educators as well as today's practitioners of genetics.
Yeast is an ideal eukaryotic microorganism for biochemical and genetic studies, particularly since its complete genome sequence became available. This Cold Spring Harbor Laboratory Yeast Genetics Course Manual combines essential standard methods with the latest technologies. In this edition, most of the experiments have been revised and several new experiments and techniques have been added, including the 2-hybrid system, PCR-based methods, and isolation of chromatin.
This is an updated edition which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents used in the study of yeast biology. Since the last edition of the manual was published (2000), revolutionary advances in genomics and proteomics technologies have had a significant impact on the field. This updated edition reflects these advances, and also includes new techniques involving vital staining, visualization of Green Fluorescent Protein, new drug resistance markers, high-copy suppression, Tandem Affinity Protein tag protein purification, gene disruption by double-fusion polymerase chain reaction, and many other recent developments.
Modern Genetic Analysis: Integrating Genes and Genomes, 2nd ed.
This edition implements an innovative approach to teaching genetics. Rather than presenting material in historical order, it integrates molecular genetics with classical genetics. This approach provides students with a foundation in molecules, while building an understanding of more abstract elements of transmission genetics. This book incorporates greater attention to pedagogy, improved chapter organization, enhanced art, and a better overall design.
Molecular Cytogenetics: Protocols and Applications
This book describes in step-by-step style the leading FISH techniques and those molecular technologies beyond FISH available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, muklti-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics.
This provides an overview of the approaches encompassed by the emerging discipline of molecular epidemiology. Concepts and methods at the forefront of infectious disease studies are discussed along with recent advances in studying host genetics, methods for the detection of mutations and polymorphisms, the relationship between pathologies and immune system genetics, and the use of DNA sequence information to garner insights into the evolution of infectious pathogens. The evolution and function of the immune system and a synopsis of general epidemiological design and analysis are included.
Molecular Genetic Analysis of Populations: A��Practical Approach
This book will be useful to those involved in screening populations to determine variation, genetic distance, gene flow, effective population size, and other related parameters.
The yeast Saccharomyces cerevisiae is used extensively in academic research and industry as a model eukaryote in molecular biology, genetics and biotechnology. This book presents methods in current use and describes and discusses experimental procedures used in a wide range of specialized topics.
Mouse Genetics and Transgenics: A Practical Approach
Covers all aspects of using the mouse as a genetic model organism: care & husbandry, archiving stocks as frozen embryos or sperm, making new mutations by chemical mutagenesis, transgenesis, gene targeting, mapping mutations, and polygenic traits by cytogenetic, genetic and physical means.
Short Protocols in Human Genetics is a compendium of methods from Current Protocols in Human Genetics, part of the well-known series that set the standard of excellence for laboratory manuals. Short Protocols in Human Genetics provides coverage of the latest methods in human genetics, including collecting family histories and pedigrees, linkage analysis, molecular genetics, physical mapping, clinical testing, and cytogenetics
Transgenesis Techniques: Principles and Protocols, 2nd ed.
This update presents techniques for generation and analysis of transgenic animals, with emphasis on manipulation of the mammalian genome. It concentrates on creation and maintenance of genetically modified murine strains, providing access to the germline by conventional pronuclear injection, and by retroviral and adenoviral infection. Emphasis is given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for constitutive and conditional gene targeting. Chapters include techniques for cryopreservation of male and female germlines and for generation of transgenic sheep by nuclear transfer.
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