 | Z702439 | A-Z of Quantitative PCR | This is not just a cookbook for real-time quantitative PCR (qPCR). Admittedly, there are lots of recipes from distinguished contributors and Bustin has attempted to collect, sift through and rationalize the vast amount of information that is available on this subject. And yes, this book was conceived as a comprehensive hands-on manual to allow both the novice researcher and the expert to set up and carry out qPCR assays from scratch. However, this book also sets out to explain as many features of qPCR as possible, provide alternative viewpoints and methods and, perhaps most importantly, aims to stimulate the researcher into generating, interpreting and publishing data that are reproducible, reliable and biologically meaningful.
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 | Z704083 | Analytical Techniques in DNA Sequencing | This text takes a look at the various DNA sequencing techniques that were developed and put to use during and after the Human Genome Project. Special attention is paid to the breakthrough Sanger Method, as well as to several other techniques that improved the accuracy, improved the detection limits, and in a variety of ways dramatically reduced the time needed to generate a DNA sequence. This volume describes the improvements in DNA separation based on advanced capillary electrophoresis and microchip sequencing. It also looks at the use of mass spectrometry and single molecule detection, and takes an in-depth look at the use of various fluorescence methods for the detection of sequencing fragments. One chapter is dedicated to chip-based microfabricated sequencing systems, which could well be the future of DNA sequencing.
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 | Z718262 | Analyzing DNA | A genome revolution is transforming biomedical research in the leading laboratories. Now, a series of manuals from Cold Spring Harbor puts the tools of the revolution in everyone's hands.
Assuming only a basic knowledge of molecular biology, these manuals explain how to clone, manipulate, analyze, and sequence large segments of DNA, and relate expressed sequence to phenotypic variation.
The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness.
A complement to the bible of recombinant DNA, Molecular Cloning, these manuals are essential for every laboratory in which genes are being studied.
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 | Z705780 | Aptamer Handbook: Fundamental Oligonucleotides and their Applications | In this book, leading scientists from academia as well as biotech and pharma companies introduce the revolutionary concept of designing RNA and DNA oligonucleotides with novel functions by in vitro selection. These functions comprise high affinity binding (aptamers), catalytic activity (ribozymes and deoxyribozymes) or combinations of binding and catalytic properties (aptazymes). Basic concepts and technologies describing in detail how these functional oligonucleotides can be identified are presented. Numerous examples demonstrate the versatility of in vitro selected oligonucleotides. Special emphasis has been put on a section that shows the broad applicability of aptamers, e. g. in target validation, for analytics, or as new therapeutics. This first overview in the field is of prime interest for a broad audience of scientists both in academia and in industry who wish to expand their knowledge on the potential of new oligonucleotide functions and their applications.
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 | Z703028 | B Cell Protocols | Recent advances in genetics, synergized by many exciting developments in cellular and molecular biology, have provided powerful new experimental tools for the study of not only B-lymphocyte development and function, but also the physiology and pathology of the immune system in higher animals. This book is a diverse collection of protocols ranging from the latest inventions and applications to some classic, but still frequently used, methods in B cell biology. These readily reproducible protocols follow the successful Methods in Molecular Biology series format.
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 | B7157 | Baculovirus Expression Vectors: A Laboratory Manual | This laboratory manual simplifies the selection of the most appropriate vector design for a given problem and describes each step in the implementation process from vector construction to large-scale protein production.
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| | Z357332 | Basic Methods in Molecular Biology, 2nd ed. | | pricing |
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| | B5678 | Basic Molecular Biology: Essential Techniques | Begins with a brief overview and then focuses on simple step-by-step protocols of the core techniques. It provides not only a theoretical outline, but also valuable advice on real-world problems and estimates on how much time is required to perform each technique.
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 | Z701718 | Bioinformatics and Functional Genomics | Encompassing the field of bioinformatics, from sequence analysis, gene expression, microarrays, and proteomics to molecular phylogenics and whole genome studies, this text shows how bioinformatics applies to biological questions at different levels, such as gene/protein, disease, and protein structures. The author emphasizes practical skills, such as how to analyze genes and proteins, how to make trees using phylogenic software, how to extract data, and how to identify genes and proteins implicated in disease. It provides a systematic three-part format, beginning with the analysis of biological sequence data, continuing with functional genomics (from microarrays to proteomics), and ending with coverage of genomes.
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| | Z718599 | Bioinformatics: Sequence and Genome Analysis | The application of computational methods to DNA and protein science is a new and exciting development in biology. Bioinformatics: Sequence and Genome Analysis is a comprehensive introduction to this emerging field of study. The book has many unique and valuable features: It is written for any biologist who wants to understand methods of sequence and structure analysis and how the necessary computer programs work Sequence alignment, structure prediction, phylogenetic and gene prediction, database searching, and genome analysis are clearly explained and amply illustrated Underlying algorithms and assumptions are clearly explained for the non-specialist Examples are presented in simple numerical terms rather than complex formulas and notation Theoretical underpinnings are linked to biological problems and their solutions Extensive tables provide descriptions and Web sources for a broad range of publicly available software. Based on the author's extensive experience as a molecular geneticist and bioinformaticist at the University of Arizona, this is a uniquely educational book, ideal as a laboratory reference for investigators and also as teaching reference for graduate and undergraduate students studying this fast-changing discipline.
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 | Z718637 | Biological Responses to DNA Damage | Living cells have evolved many ways of coping with metabolic events and environmental influences that damage DNA. These mechanisms, and the frequent progression to cancer that results when they go awry, are reviewed in this volume by authors from over sixty of the world’s leading laboratories. The topics discussed include DNA repair, mutagenesis and other damage-tolerance functions, checkpoint control, apoptosis, and adaptation. They draw from studies on human and yeast cells. Current, but with a valuable historical perspective, this volume has the depth and lasting value typical of this most prestigious series and is essential reading for investigators of DNA replication, cell cycle control, and tumorigenesis.
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 | Z718645 | Biological Responses to DNA Damage | Living cells have evolved many ways of coping with metabolic events and environmental influences that damage DNA. These mechanisms, and the frequent progression to cancer that results when they go awry, are reviewed in this volume by authors from over sixty of the world’s leading laboratories. The topics discussed include DNA repair, mutagenesis and other damage-tolerance functions, checkpoint control, apoptosis, and adaptation. They draw from studies on human and yeast cells. Current, but with a valuable historical perspective, this volume has the depth and lasting value typical of this most prestigious series and is essential reading for investigators of DNA replication, cell cycle control, and tumorigenesis.
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 | B7183 | Biologist’s Guide to Analysis of DNA Microarray Data | This is the first authoritative text to focus on microarray analysis (as opposed to technology) in diverse biological and medical applications. Written for biologists without special training in data analysis and statistics, the Guide takes over where the image analysis software that accompanies DNA array equipment typically leaves off-with a file of signal intensities and fold changes compared to a control. The broad spectrum of established analysis approaches is covered, including cluster analysis, function prediction, and principal component analysis. A thorough, critical review of software programs is presented.
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 | Z702838 | Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory | This book is the first comprehensive guide devoted exclusively to calculations encountered in the genetic engineering laboratory. This guide, written for students, technicians, and scientists, provides example calculations for the most frequently confronted problems encountered in gene discovery and analysis. The text and sample calculations are written in an easy-to-follow format. It is the perfect laboratory companion for anyone working in DNA manipulation and analysis.
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| | Z364320 | Cell and Molecular Biology: Essential Data Series | This handy reference brings together a wide range of core information needed by all researchers in the biomolecular sciences and additional selected information from the areas of biochemistry, cell biology, genetics and molecular biology.
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 | C1854 | Chromatin Protocols | Collection of protocols is designed to analyze the relationship between chromatin structure and function, and to elucidate molecular mechanisms that control vital cellular functions such as transcription, replication, recombination, and DNA repair. Methods cover topics from cell-free systems for the constitution of chromatin heterogeneity in vitro, to techniques for in vivo analysis of protein–DNA interactions. Methods include instructions to ensure successful replication and to avoid pitfalls.
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 | C5224 | Chromatin: A Practical Approach | This title provides clear explanations of a wide range of techniques from the initial isolation and fractionation of chromatin and linker histones, to the in vitro assembly of chromatin and nuclear structures in Xenopus egg extracts.
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 | C4862 | Cloning and Expression Vectors for Gene Function Analysis | This describes the latest modifications to important and original research initially published in the leading methods journal BioTechniques. This new volume brings together, in one collection, some of the best articles published in the exciting field of gene cloning and protein expression and presents updated protocols selected from some of the most widely used techniques practiced in the modern molecular biology laboratory. Specific chapters include the latest information on constructing cDNA libraries, PCR conditions, choosing the correct expression system, and purification strategies.
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 | Z700592 | Cloning, Gene Expression, and Protein Purification: Experimental Procedures and Process Rationale | This resource presents protocols for isolating a gene, cloning and characterizing it, expressing its encoded protein, and purifying and characterizing the protein's physical properties. It includes background and procedures and is structured around 20 experiments that demonstrate how to prepare, manipulate, and analyze plasmids, produce fusion proteins in bacteria, and purify these proteins based on chemical properties or substrate affinities. It describes topics such as the use of antibodies and techniques developed to transform their structures, and approaches designed to manipulate structure and functions of proteins and nucleic acids.
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 | Z718475 | Coiled Spring: How Life begins soft cover | A concise, readable account of the principles underlying embryonic and appendage development in animals and plants, illustrated with colorful artwork and interviews with prominent investigators. Highly recommended for use in undergraduate and graduate courses.
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 | Z718467 | Coiled Spring: How life begins hard cover | A concise, readable account of the principles underlying embryonic and appendage development in animals and plants, illustrated with colorful artwork and interviews with prominent investigators. Highly recommended for use in undergraduate and graduate courses.
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 | Z704881 | Condensed Protocols from Molecular Cloning: A Laboratory Manual | Molecular Cloning: Condensed Protocols is a single-volume adaptation of the three-volume third edition of Molecular Cloning: A Laboratory Manual. The book contains only the step-by-step portions of the protocols, accompanied by the appendices. Each protocol is cross-referenced to the appropriate pages in the original book. This affordable volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the world's best-selling manual of molecular biology techniques.
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| | Z705357 | Condensed Protocols from Molecular Cloning: A Laboratory Manual Hardback | Molecular Cloning: Condensed Protocols is a single-volume adaptation of the three-volume third edition of Molecular Cloning: A Laboratory Manual. The book contains only the step-by-step portions of the protocols, accompanied by the appendices. Each protocol is cross-referenced to the appropriate pages in the original book. This affordable volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the world's best-selling manual of molecular biology techniques.
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 | C2606 | Cytokine Molecular Biology: A Practical Approach | This manual concentrates on molecular biology techniques for the study of cytokines, cytokine receptors, and cytokine driven processes. Topics include the cloning and expressing of cytokine genes, the analysis of cytokine mRNA by RT-PCR, cDNA array systems and in situ hybridization, the study of cytokine gene polymorphisms, the analysis of cytokine signaling via PC-specific phospholipase C and sphingomyelinases, the Jak/STAT and MAPK signaling pathways, and gene modification in transgenic and mutant mice.
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 | Z700320 | DNA Array Image Analysis: Nuts & Bolts | DNA Microarrays are an indispensable tool in molecular biology, which allows researchers to gain insight into the intricate mechanisms of gene expression. Common to all array-based technologies is the necessity to analyze digital images of the scanned DNA array. This book emphasizes aspects of both theory and application. It offers the basics of machine vision and image analysis for biologists, "how-to" approach to microarray image analysis, and practical tips for both the novice in the field and the advanced user.
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 | D6064 | DNA Arrays: Methods and Protocols | Microarray technology is used in research of gene expression, mutation analysis, proteomics and gene sequencing. Protocols provide instruction in designing and constructing DNA arrays as well as hybridizing them with biological samples for analysis. Details include how to attach or print arrays on various matrices, biological sample preparation (DNA and RNA), hybridization conditions, signal detection, probe optimization, different printing technologies and data collection and analysis. Other topics are genotyping, sequencing by hybridization, antisense reagents, gene expression analysis, and business aspects of biochip technology.
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 | D5565 | DNA Arrays: Technologies and Experimental Strategies | A giant leap in technology took place with the introduction of cDNA arrays that have been developed in response to the need for simultaneous analysis of the patterns of expression of thousands of genes. This book offers a view of different aspects of this rapidly developing technology, including oligonucleotides and electrochemical arrays. Up-to-date insights into methodological fundamentals of construction of DNA arrays, optimization of hybridization conditions, the use of expression data in bioinformatics, different software tools, and the application of microarray technology for drug discovery process are provided.
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| | Z362360 | DNA Cloning: A Practical Approach, 2nd ed. Volume 1: Core Techniques | Topics include transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and gene expression, in vitro mutagenesis, and DNA sequencing.
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| | Z362379 | DNA Cloning: A Practical Approach, 2nd ed. Volume 2: Expression Systems | This volume provides essential information and full details of current procedures for the expression of cDNA clones in E. coli, lambda-phage, yeast, and baculovirus expression systems. Also covered are the isolation and purification of recombinant protein(s), and the generation of monoclonal and polyclonal antibodies.
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| | Z306193 | DNA Cloning: A Practical Approach, 2nd ed. Volume 3: Complex Genomes | | pricing |
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| | Z306207 | DNA Cloning: A Practical Approach, 2nd ed. Volume 4: Mammalian Systems | Topics covered include: high efficiency gene transfer into mammalian cells, use of vectors based on gene amplification, retroviral vectors, genetic manipulation of embryonic stem cells, production of transgenic rodents, genomic and expression analysis of transgenic animals, expression using a defective HSV-1 vector system, and adenovirus vectors.
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| | Z719005 | DNA From The Beginning | DNA from the Beginning (a 3–CD set) is a multimedia primer on the basics of DNA, genes, and heredity. It is an ideal teaching tool for high school students and will also interest adults with a non-technical background who wish to learn more about the impact that DNA science is having on our lives.
The content in DNA from the Beginning is built on a progressive series of important but simply stated concepts such as Children resemble their parents. An understanding of the concept is then conveyed in clear,understandable terms with the aid of links to animations, photographs, and video clips, and biographies or interviews featuring key scientists. A wealth of other information and links to web resources are included. Problems and their solutions are included to allow teaching through self-examination.
Based on the award-winning web site, the DNA from the BeginningCD–ROM set provides over 72 hours of study time. It runs on Macintosh computers and PCs, and gives immediate access to all the online content, in attractive, uncluttered screens, without the need for network connections.
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| | Z719013 | DNA Interactive DVD | DNA Interactive (DNAi) is a multimedia resource for high school and introductory college biology that shows how we came to understand the language of DNA, how we bend it to our own service, and what it can tell us about who we are. The DNAi products an award winning website (http://www.dnai.org ) and the DVDare the result of a two year collaboration between scientists, educators, and film makers on three continents. It includes more than 200 video clips and animations; video interviews with 11 Nobel Laureates and more than 50 other scientists, clinicians, and patients; and spectacular 3 D animations that illustrate intracellular processes and explore aspects of DNA science.
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 | D4691 | DNA Methylation Protocols | This book offers a set of readily reproducible protocols of the analysis of DNA methylation and methylases. These powerful methods provide the tools necessary for studying methylation at both the global level and the level of sequence, and include many techniques for identifying genes that might be aberrantly methylated in cancer and aging. Additional methods cover genome-wide analysis of abnormal DNA methylation and the isolation and measurement of demethylases and related proteins.
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 | Z704199 | DNA Methylation: Approaches, Methods and Applications | A guide to understanding the complex regulatory roles of DNA methylation, this volume describes the relation that this biological mechanism has to gene silencing in disease and explores its promising role in treating cancer. The book is a state-of-the-art reference for understanding the role DNA methylation plays in human disease, particularly cancer. This compilation of articles outlines the best techniques to use when addressing questions concerning the cytosine methylation status of genomic DNA. It includes concepts, experimental models, and clinical uses of demethylating agents. The book provides a balance between articles clarifying methodological details and more general review chapters.
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 | D5440 | DNA Microarrays: A Molecular Cloning Manual | DNA microarray, or DNA chip technology is a new and powerful means of discovering, characterizing and analyzing genes and their expression patterns. This manual assembles and synthesizes the expertise of over 30 innovators in this emerging field to provide authoritative, detailed instruction on the design, construction, and applications of microarrays, as well as comprehensive descriptions of the software tools and strategies required for extensive image and data analysis. It complements the best selling manual "Molecular Cloning".
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 | Z718750 | DNA Microarrays: A Molecular Cloning Manual | DNA microarray technology is a new and powerful means to analyze genomes and characterize patterns of gene expression. Its applications are widespread across the many fields of plant and animal biological and biomedical research. This manual, designed to extend and to complement the information in the bestselling Molecular Cloning, is a synthesis of the expertise and experience of more than 30 contributors - all innovators in a fast-moving field. DNA Microarrays provides authoritative, detailed instruction on the design, construction, and applications of microarrays, as well as comprehensive descriptions of the software tools and strategies required for analysis of images and data.
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 | D6187 | DNA Microarrays: A Practical Approach | Written and edited by experts in the field, this book provides valuable insight into the remarkable advancement of DNA microarrays. It is essential for researchers investigating patterns of gene expression and useful for all those interested in the use of biochips.
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 | Z700185 | DNA Microarrays: Gene Expression Applications | This complete and practical manual on expression measurement using DNA arrays covers the existing methods (from nylon macroarrays to oligonucleotide chips) and includes detailed protocols. It has been written by scientists who have experienced the difficulties involved in actually using microarrays, and provides helpful advice and hints on setting up these powerful but tricky methods. Software, data mining procedures and probable future developments, which should be useful to anyone interested in expression measurement, are also covered. It provides detailed protocols as well as many helpful hints to achieve experimental success and to avoid pitfalls.
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| | Z719293 | DNA Microarrays: Methods Express Series Hardcover | DNA Microarrays: Methods Express covers the very latest in DNA microarray technology, with a clear focus on how these techniques can be used in the lab to gain the very best results. The authors are from some of the leading laboratories in the field and write with real authority on the latest methodology and its applications. Every chapter provides detailed step-by-step protocols with valuable hints and tips for success, as well as giving typical experimental results and selected literature citations.
THIS BOOK WILL BE PUBLISHED IN THE FUTURE, SIGMA-ALDRICH CAN ACCEPT ORDERS NOW FOR FULLFILLMENT WHEN PUBLISHED.
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| | Z719307 | DNA Microarrays: Methods Express Series Softcover | DNA Microarrays: Methods Express covers the very latest in DNA microarray technology, with a clear focus on how these techniques can be used in the lab to gain the very best results. The authors are from some of the leading laboratories in the field and write with real authority on the latest methodology and its applications. Every chapter provides detailed step-by-step protocols with valuable hints and tips for success, as well as giving typical experimental results and selected literature citations.
THIS BOOK WILL BE PUBLISHED IN THE FUTURE, SIGMA-ALDRICH CAN ACCEPT ORDERS NOW FOR FULLFILLMENT WHEN PUBLISHED.
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 | D7937 | DNA Profiling and DNA Fingerprinting | This book reflects practical approaches to answering a variety of biological and medical questions using DNA fingerprinting and genetic profiling in a broad sense that also includes statistical evaluation of the data. It has been written for all researchers in the theoretical and applied sciences wherever genetic identification and relationship analysis are required.
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 | Z704784 | DNA Repair : Mammalian Systems, 2nd ed. | This collection of reproducible techniques for repairing mammalian DNA contains 14 entirely new chapters and many extensively revised chapters. The methods presented cover cytogenetic analysis, measuring the cellular response to ionizing radiation, detecting single-strand (nicks) and double-strand DNA breaks, detecting the presence of “adducted” bases in DNA, and preparing mismatch repair (MMR) plasmid substrates. Among the highlights are excellent coverage of both base excision repair (BER) and nucleotide excision repair (NER), useful assays for identifying and quantifying UV-induced DNA lesions and DNA breakage, gene therapy, environmental mutagenesis and cancer, and gene targeting.
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 | D6561 | DNA Repair Protocols: Eukaryotic Systems | Instructions are provided for analyzing DNA repair processes and cellular responses to DNA damage, including nucleotide and base excision repair, DNA strand break repair, and mismatch repair. Methods focus on eukaryotic model systems that have made, or may make, contributions to the understanding of cellular responses to DNA damage in relation to mutagenesis, carcinogenesis, and cell cycle. Non-mammalian systems as yeast, C. elegans, Drosophila, Xenopus, and plants are considered.
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 | D8436 | DNA Repair Protocols: Prokaryotic Systems | This is a collection of protocols from leaders in the field. First section deals with identification, purification and characterization of DNA repair enzymes. Repair pathways include base excision repair, mismatch repair and recombination repair as it explores activities and characterization of specific DNA repair proteins. Second section provides protocols for applied use of DNA repair proteins in molecular biology techniques including mutation detection, cloning and genome diversification.
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 | Z369888 | DNA Repair and Mutagenesis | A major reference work for molecular biologists working in carcinogenesis, gene regulation, environmental studies, and molecular evolution. The text details the mechanisms by which DNA can be damaged and the strategies that organisms have developed to mitigate the effects of that damage. The concluding chapter discusses human diseases that result from defective DNA repair processes.
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 | Z702501 | DNA Repair in Cancer Therapy | The authors explain in detail the various mechanisms by which cancer cells can circumvent anticancer therapy. They also review the clinical impact of such novel inhibitors of DNA repair mechanisms as methylguanine-DNA-methyltransferase. Also examined are inhibitors of other DNA repair enzymes such as PARP and DNA-PK, now under development and close to clinical trials. The book captures for both cancer researchers and practicing oncologists dealing with hallmark "relapse" or "drug resistance" phenomena on a daily basis-the many exciting new uses of DNA repair inhibitors, either alone or in combination with anticancer therapies
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| | Z378437 | DNA Replication in Eukaryotic Cells | An international panel of contributors combine to create this exhaustive treatise. General topics include origins of replication, priming, role of transcription factors in replication, fidelity and repair, chromatin structure, and comparisons to prokaryotic mechanisms. A section on proteins includes chapters on cellular and viral DNA polymerases, accessory proteins, ligases, topoisomerases, and telomerases. Finally, about half the book is devoted to discussion of specific replication systems including eleven viruses, yeast, insects, plants, mammals, organelles, and more.
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 | Z705462 | DNA Sequencing II: Optimizing Preparation and Cleanup | Kieleczawa's second volume is devoted to the various methods used for extraction, clean-up, quantification, and analysis of DNA. This volume is divided into four comprehensive sections - DNA Purification, Clean-up of DNA Fragments, Storage of DNA, and Quantifying DNA and RNA - and offers the reader an in-depth presentation of DNA technologies. The text also touches upon the many tools and software programs that are found in a typical modern biology laboratory. This fascinating text is a great addition to your molecular biology library.
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 | D4814 | DNA Sequencing Protocols, 2nd ed. | Major advances have made PCR-based semiautomated fluorescent sequencing the norm. This new edition provides up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. It offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. It offers easy-to-follow methods that will improve the accuracy and quality of DNA sequences obtained by smaller laboratories and help lay the foundation for molecular diagnostics.
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 | Z373850 | DNA Sequencing Strategies | With more than 100 charts, tables, and photographs, this book encompasses many important advancements and developments. Designed as an easy to use bench reference, it guides you through step-by-step sequencing processes, refining techniques, and research requirements.
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 | Z702870 | DNA Sequencing: Optimizing the Process and Analysis | DNA sequencing techniques have evolved rapidly in recent years, but scientists still encounter challenges in sequencing genomes. This book was written to help troubleshoot these difficulties while also providing a practical guide for those routinely sequencing DNA to refine and enhance their operation. This volume covers important topics in the field, including: biochemical and technological advances induced by the Human Genome Project; proven and newly emerging methods of preparing DNA templates; effects of some widely used laboratory reagents on DNA sequencing; Laboratory Information Management Systems (LIMS); and the future of DNA sequencing.
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 | Z340715 | DNA Viruses: A Practical Approach | This book groups together the major experimental methods currently employed to study DNA viruses, from the fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and realtime PCR analysis of drug resistance mutations in clinical isolates. Topics include virus genome mapping, sequence analysis,, viral cytokine-binding proteins, analysis of virus replication, and neoplastic transformation.
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 | Z705136 | DNA Viruses: Methods and Protocols | A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, this book will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.
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 | D2936 | DNA-Protein Interactions: A Practical Approach | This is a novel compilation of methods for studying the interactions of proteins with DNA. Multidisciplinary approaches are valuable for solving problems and obtaining a detailed understanding of the molecular regulatory interactions involved. It covers all of the major tools that are required for the study of the large macromolecular enzymatic machines that manipulate DNA, with particular emphasis on biophysical techniques applied to the analysis of transcription and its relation to chromatin structure.
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 | D6689 | DNA-Protein Interactions: Principles and Protocols, 2nd ed. | Revised second edition provides the only book exclusively concentrating on up-to-date techniques for protein-DNA interactions. Easy-to-follow protocols illuminate the molecular basis of protein-nucleic acid interactions. Use them successfully to reveal location of DNA binding site, strength and specificity of a binding, identities of individual groups on actual bases involved in binding, and specific amino acid residues of the protein that interact with DNA. Some techniques can be used to identify previously unknown DNA binding proteins from crude cell extracts.
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 | Z704407 | Differential Display Methods and Protocols, 2nd ed. | The second edition consists of a wider-ranging collection of chapters that highlight both recent methodological refinements and some fine examples of differential display (DD) applications in recent years. Part I covers gene discovery methodologies that have a unique place in the modern molecular biological toolbox for gene expression analysis while Part II demonstrates the power of DD technology by showcasing select applications that have led to the discovery of many important genes involved in viral infection, Prion disease, cancer, ovulation, circadian clock, floral color, transcription re3pression, gene silencing, mRNA polymorphism and protein-RNA interaction.
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 | Z718742 | Discovering the Double Helix | The structure of DNA proposed by James Watson and Francis Crick in 1953 was one of the most important scientific discoveries of the twentieth century, transforming biology, giving medicine new impetus, and providing a foundation for the new industry of biotechnology. Jim Watson’s best-selling memoir of the events leading to this discovery, The Double Helix, has enthralled millions of readers since its publication in 1968. In this videotaped lecture, recorded live at Cold Spring Harbor Laboratory in November 1999, Dr. Watson retraces those events in a very personal reminiscence of the period and the people involved. This is an account of one of the great dramas of science, told from the intimate perspective of a participant who, aged 25 at the time of the discovery, has become one of the intellectual leaders of our time. James D. Watson was Director of Cold Spring Harbor Laboratory, New York, from 1968 to 1993 and is now its President. He was the first Director of the National Center for Human Genome Research of the National Institutes of Health from 1989 to 1992. Dr. Watson is a member of the National Academy of Sciences and Royal Society and has received many awards and honors, including the Presidential Medal of Freedom and the National Medal of Science. In addition to The Double Helix, his books include Molecular Biology of the Gene, Molecular Biology of the Cell, Recombinant DNA, and A Passion for DNA.
PAL versions 60 minutes
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 | Z718173 | Double Talking Helix Blues | "...the point of this story, I'll tell you right now. Did you ever sit down and think about how It is that each time a baby's born It's a baby -- not a rabbit or an ear of corn?" The answer is in DNA -- the stuff that genes are made of. Twin brothers Joel and Ira Herskowitz, physician and scientist, respectively, have come up with a new way to explain to children (ages 8) how it is that when a person's genetic message is "copied," the result is another human being -- not a rabbit or an ear of corn. Based on a song written by Joel Herskowitz while he was in medical school, the "Double Talking Helix Blues" is a book and audio tape presentation on the structure and function of DNA. The book includes the text for Joel's song, which is illustrated with spectacular paintings by Judy Cuddihy to provide an important visual component to the overall "lesson" on DNA. This is followed by a guide written by Ira Herskowitz to explain the scientific terms and concepts in the song; this section describes in clear terms what, for example, a chromosome, nucleus, and molecule are. Accompanying the book is a 12-minute audio tape with performances of the song by Joel and Ira. All in all, the "Double Talking Helix Blues" provides young readers with a wonderful introduction to concepts of cells and molecules, reproduction at the molecular level, and DNA and its structure. The target age group for this presentation is ages 8 and up, as well as teachers, scientists, and all those interested in molecular biology at its most basic level.
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| | Z358177 | Encyclopedia of Molecular Biology | Short entries which provide a basic definition are cross-referenced to longer articles that place the definition in context and provide essential background. Current literature citations facilitate further research.
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 | Z379689 | Endothelin: Molecular Biology, Physiology, and Pathology | Academic, clinical, and pharmaceutical researchers summarize the dramatic recent progress in endothelin research and speculate on future studies. The respected contributors critically evaluate and synthesize our knowledge of endothelin chemistry, its mechanism of action, and its physiological function. Biochemists studying the role of endothelin in cardiovascular physiology and medicinal chemists developing endothelin-centric drugs will find the book rich in insights.
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 | Z700703 | Erythropoietin: Molecular Biology and Clinical Use | Erythropoietin has been the fountainhead in research on molecular mechanisms of oxygen-sensing and hypoxia-induced gene expression. The availability of recombinant human EPO for prevention and therapy of chronic anemias was a turning point in clinical medicine. This book intends to provide a fairly complete overview and bibliography of the progress made in important areas of basic and clinical EPO research. It is divided into sections on EPO structure and control of production; EPO mechanisms of action; EPO physiology and pathophysiology; EPO pharmacology and therapy; and recombinant human EPO therapy.
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 | E8390 | Essential Molecular Biology: A Practical Approach, Volume 1, 2nd ed. | There are two books in this series. Volume 1 concentrates on the procedures for DNA and RNA manipulation, purification, electrophoresis and the construction and cloning of recombinant molecules. It also includes a survey of cloning vectors of Escherichia Coli. As with the first edition, the objective is to combine solid practical information with sufficient background material to meet the requirements of any enduser
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 | E3653 | Essential Molecular Biology: A Practical Approach, Volume 2, 2nd ed. | In Volume 2 of Essential Molecular Biology, procedures for preparing gene libraries and identifying genes are described, together with methods for studying the structure of a cloned gene and the way it is expressed in the cell.
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 | Z701343 | Essentials of Molecular Biology, 4th ed. | This edition focuses on the fundamental aspects of molecular structure and function by reviewing key features and along the way, capsulizing them as a series of concise concepts. Thus, one is encouraged to place the reductionist knowledge of molecular biology into broad contexts. Module themes include The Structure of Proteins, Nucleic Acids, and Macromolecular Complexes; Function of Macromolecules; Coordination of Macromolecular Function in Cells; Experimental Manipulation of Macromolecules
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 | Z374016 | Eukaryotic DNA Replication | This book begins with reviews of the molecular and genetic compounds of the replication machinery, and builds into a detailed picture of how the replication process is regulated within the cell. A very timely description of the latest research findings in this key subject area.
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| | Z375675 | Experiments in Molecular Biology: Biochemical Applications | Provides a thorough introduction to recombinant DNA methods used in molecular biology and nucleic acid biochemistry.
Contains student tested, easy to follow protocols
• Provides both detailed descriptions of the experiments and a theoretical support section that reinforces the principles behind the methods presented
• Sequentially links experiments to provide a ″project″ approach
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| | Z730858 | Expression Systems | Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when. This book covers expression across a broad range of systems, including the following. *Baculovirus expression vectors, *CHO cells, * E. coli, *HEK293-EBNA1 cells, * Lactococcus lactis, * S. cerevisiae, *transfected insect cells, * Pichia pastoris, *mammalian cells using BacMam viruses, *lentiviral vectors, *wheat germ cell-free system. The book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression.
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 | Z705683 | Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols | In the first comprehensive treatment of energy transfer (ET) nucleic acid probes, experts thoroughly describe all the major probes, both fluorescence resonance energy transfer (FRET)-based and non-FRET-based, and provide a complete set of techniques to monitor DNA and RNA reactions, including hybridization, amplification, cleavage, folding, and associations with proteins, other molecules, and metal ions. Optimal design strategies for customized ET probes are presented, as well as techniques for distance determination in protein–DNA complexes and the detection of topological DNA alterations, mutations, DNA breaks and single nucleotide polymorphisms. Authors describe in detail the design and application of ET-using molecular devices, such as biosensors, molecular machines, and logic gates for molecular scale computation.
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 | Z701114 | Functional Genomics: Methods and Protocols | This collection of robust, readily reproducible methods for microarray-based studies includes expert guidance in the optimal data analysis and informatics. On the methods side are proven techniques for monitoring subcellular RNA localization en masse, for mapping chromosomes at the resolution of a single gene, and for surveying the steady-state genome-wide distribution of DNA binding proteins in vivo. For those workers dealing with massive data sets, the book discusses the methodological aspects of data analysis and informatics in the design of microarray experiments, the choice of test statistic, and the assessment of observational significance, data reduction, and clustering.
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 | Z730351 | Gene Cloning: Principles and Applications | The ability to successfully clone genes underlies the majority of our knowledge in molecular and cellular biology. Existing textbooks describe what has been learnt from cloned genes but include little detail on how the cloning was achieved and how the techniques involved are carried out in the laboratory. Gene Cloning introduces the diverse array of techniques available to clone genes and how they can be used effectively both in the research laboratory, to gain knowledge about the gene, and for use in biotechnology, medicine, the pharmaceutical industry, and agriculture. It shows how cloning genes is an integral part of genomics and underlines its relevance in the post-genomic age, as a tool required to test predictions experimentally.
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 | Z702358 | Gene Expression Profiling: Methods and Protocols | Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application
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 | Z373974 | Gene Isolation and Mapping Protocols: Methods in Molecular Biology, Vol. 68 | A collection of the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using data base analyses. Also, numerous time-saving techniques and informative explanations about certain steps are included.
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 | G5916 | Gene Knockout Protocols | Highly skilled investigators in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for the analysis of the mouse phenotype. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. State-of-the-art and highly practical, it constitutes an invaluable source of readily reproducible techniques for genetic researchers today.
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 | Z730165 | Gene Mapping, Discovery, and Expression: Method and Protocols | A collection of cutting-edge computational tools and experimental techniques to study how genes are regulated, and to reconstruct the regulatory networks through which various cell-types are produced. On the computational side, web-based technologies to localize genes, to access and retrieve data from microarray databases, to conduct comparative genomics, and to discover the potential “codes” in genomic DNA that may control the expression of protein-coding genes. Detailed experimental techniques described include methods for studying chromatin structure and allele-specific gene expression, methods for high-throughput analysis to characterize the transcription factor binding elements, and methods for isolating and identifying proteins that interact with DNA.
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| | Z306177 | Gene Probes 1 | Over 85 key protocols for the synthesis, labeling, detection, and quantification of DNA, RNA, oligodeoxynucleotide, and antibody probes are provided. It brings together key practical advice and step-by-step protocols for all of the major methods of probe labeling and detection.
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 | Z306185 | Gene Probes 2 | Over 100 protocols for using DNA, RNA, oligodeoxynucleotide, and antibody probes in screening gene libraries, mapping, gene structure determination, analysis of gene expression, and mutational studies, are presented.
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 | G1793 | Gene Probes: Principles and Protocols | Volume 179 of the Methods in Molecular Biology series contains an outstanding collection of time-tested protocols for designing and using genes probes in wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions
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 | Z730327 | Gene Regulation 5th ed. | Gene regulation is an essential process in the development and maintenance of a healthy body. This Fifth Edition provides a clear, up-to-date description of gene regulation in eukaryotes, distilling the vast and complex primary literature into a concise overview. For this edition, in addition to updating of existing material, sections on important methodologies have been expanded, and a new section included on regulation by small interfering RNAs. More detail has been added on the role of multi-protein complexes in transcriptional activation, and to the discussion of the regulation of transcription factor activity by specific modifications, to include acetylation and ubiquitination, as well as phosphorylation. The final chapter on gene regulation and human disease now includes additional novel examples, and a new section on the therapeutic potential of gene regulation.
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 | Z340790 | Gene Targeting: A Practical Approach, 2nd Edition | This updated version includes the major new techniques and tried-and-tested practical protocols. It concentrates on gene targeting in mouse ES cells, but the techniques can be adapted to applications in tissue culture, including human cells.
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 | G8416 | Gene Transfer Methods: Introducing DNA Into Living Cells and Organisms | A critical aspect of the molecular revolution is that DNA frequently must be re-introduced into living cells to determine activity. The goal of this book is to compile a core set of numerous DNA transfer methods within a single volume. Chapters discuss DNA transfer into cells of bacterial, fungal, and mammalian origin, as well as into the germlines of flies, mice, and plants. Each chapter includes supplementary information such as common problems and specific troubleshooting suggestions. Progress and hurdles in the field of human gene therapy are presented.
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 | Z703729 | Gene Transfer to Animal Cells | Gene transfer to animal cells was first achieved more than 30 years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. This book describes the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. The final chapter provides an overview of all the applications of animal cell transformation.
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 | Z730033 | Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual | Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. This book provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response.
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| | P5980 | Gene cloning and analysis by RT-PCR | This new laboratory protocols manual provides investigators with up-to-date RT-PCR based methods for gene cloning and analysis. Since RT-PCR was first described for the detection of low-abundance mRNAs, the method has been extended to quantitative measurements of relative and absolute levels of single and multiple mRNAs. Methods for obtaining RNA for RT-PCR have also been extended to include analysis of mRNA levels in clinical samples and even in single cells.
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 | Z700991 | Generation of cDNA Libraries:Methods and Protocols | Expert researchers and inventors in the field describe their own proven techniques for generating cDNA/mRNA libraries to identify the functions of specific decoded gene sequences. A wide variety of techniques is presented for enhancing the generation of complete and full-length libraries, and for confirming the quality of the cDNAs generated. Among the applications detailed are electrophoresis, Northern blotting, single cell microarray analysis, subtractive hybridization, subtractive cloning, gene cloning, and peptide library generation. Each method includes background information, step-by-step instructions, a list of reagents, operational tips, and notes on instrumentation.
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 | Z702420 | Genes VIII | Two decades ago Benjamin Lewin's Genes revolutionized the teaching of molecular biology and molecular genetics by introducing a unified approach to bacteria and higher organisms. Genes has remained at the cutting edge of molecular biology, covering gene structure, organization, and expression. Originally the text opened with the genetic code and worked toward genome structure. Genes VIII changed the approach to begin with the sequence of the human and other genomes and starts with complete coverage of recent advances in genomics. The coverage of genomics is then integrated throughout the text.
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 | Z703516 | Genetic Variance Detection: Nuts & Bolts of DHPLC in Genomics | Studies of sequence variation are an integral part of genome research, because of differences in the genomic sequences between individuals. Variations in mammalian genomic sequences occur at an estimated frequency of at least one base per thousand bases. In the case of the human genome, this totals more than three million variances. These variances are typically present in the form of single nucleotide polymorphisms (SNPs). The relationship between the variability in the sequence of specific genes among individuals to their susceptibility to disease is the key to understanding gene function. This book focuses on a technology used for screening of unknown sequence variants, namely denaturing high-performance liquid chromatography (DHPLC).
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 | Z703982 | Genetic Variance Detection: Technologies for Pharmagenomics | This book broadly covers a wide range of technologies currently used for genetic variance detection and constitutes an essential contribution to the field. A comprehensive reference for scientists studying DNA in organisms compares and evaluates the technological choices available in detecting genetic variances in pharmacogenomics. Commonly used technologies such as sequencing, DNA array and chip technologies, mass spectrometry, gel- and chromatography-based technologies, as well as less commonly used methods are described in detail and illustrated with specific examples. Expert advice is presented on technological options to facilitate decisions about the utility and applicability of individual technologies for specific needs. A reference guide for every molecular biology laboratory.
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 | Z378410 | Genome Analysis: Laboratory Manual Series Volume 1: Analyzing DNA | This volume presents exhaustive coverage of techniques for: purifying genomic DNA; manipulating high-molecular weight DNA; several specialized DNA sequencing methods; and computational analysis of DNA and protein sequences. Appendices include recipes for common reagents, basic procedures, safety, and a compilation of ″useful facts.″
Assuming only a basic knowledge of molecular biology, these manuals explain how to clone, manipulate, analyze, and sequence large segments of DNA, and relate sequence to phenotype variation. Techniques, written for human as well as other animal genomes, deal plainly with sources of failure and how to avoid them. Protocols are written by experts, often by those who created the methods. The series is an excellent complement to the bible of recombinant DNA, Molecular Cloning.
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 | Z382663 | Genome Analysis: Laboratory Manual Series Volume 2: Detecting Genes | Topics include Strategies for gene discovery in mammalian systems; Constructing and screening normalized cDNA libraries; Direct cDNA selection; Exon trapping; and Detection of DNA variation
Assuming only a basic knowledge of molecular biology, these manuals explain how to clone, manipulate, analyze, and sequence large segments of DNA, and relate sequence to phenotype variation. Techniques, written for human as well as other animal genomes, deal plainly with sources of failure and how to avoid them. Protocols are written by experts, often by those who created the methods. The series is an excellent complement to the bible of recombinant DNA, Molecular Cloning.
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 | G5039 | Genome Analysis: Laboratory Manual Series Volume 3: Cloning Systems | Assuming only a basic knowledge of molecular biology, these manuals explain how to clone, manipulate, analyze, and sequence large segments of DNA, and relate sequence to phenotype variation. Techniques, written for human as well as other animal genomes, deal plainly with sources of failure and how to avoid them. Protocols are written by experts, often by those who created the methods. The series is an excellent complement to the bible of recombinant DNA, Molecular Cloning.
Topics include Bacterial Cloning Systems; Cosmids; Cloning into Bacteriophage P1 Vectors; OBacterial Artificial Chromosomes; and Yeast Artificial Chromosomes
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 | Z382698 | Genome Analysis: Laboratory Manual Series Volume 4, Mapping Genomes | Topics include
Meiotic Mapping in Humans;
Genetic and Comparative Mapping in Mice;
Identification and Analysis of DNA Polymorphisms;
DNA Markers for Physical Mapping;
Representational Difference Analysis;
Somatic Cell Genetics and Radiation Hybrid Mapping;
Fluorescence In Situ Hybridization
Assuming only a basic knowledge of molecular biology, these manuals explain how to clone, manipulate, analyze, and sequence large segments of DNA, and relate sequence to phenotype variation. Techniques, written for human as well as other animal genomes, deal plainly with sources of failure and how to avoid them. Protocols are written by experts, often by those who created the methods. The series is an excellent complement to the bible of recombinant DNA, Molecular Cloning.
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 | Z730335 | Genomes 3 | Genomes 3 builds on the achievements of the previous two editions by putting genomes, rather than genes, at the center of molecular genetics teaching. Recognizing that molecular biology research was being driven more by genome sequencing and functional analysis than by research into genes, this approach has gathered momentum in recent years. The artwork has been entirely re-drawn with the emphasis throughout on simplicity and scientific consistency. This edition includes two new chapters on genome anatomies, covering the latest sequencing achievements; new chapter on genome function, to describe the latest technologies used in studying the transcriptome and the proteome; new chapter on recombination, providing expanded coverage of genome evolution; Free CD-ROM of the artwork supplied with every copy.
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 | G0793 | Genomics Protocols | This book contains a comprehensive generic set of protocols for gene hunting and genome analysis. Drawing on emerging technologies in the fields of bioinformatics and proteomics, these protocols cover traditional genomics, and early therapeutic approaches exploiting the potential of gene therapy. Highlights include methods for the analysis of differential gene expression, SNP detection, comparative genomic hybridization, and the functional analysis of genes, as well as the use of bioinformatics for gene identification and the prediction of protein function.
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 | Z718939 | George Beadle, An Uncommon Farmer: The Emergence of Genetics in the 20th Century | George Beadle was a towering scientific figure whose work from the 1930s to 1960 marked the transition from classical genetics to the molecular era. Among other distinctions, he made the pivotal, Nobel Prize–winning discovery with Edward Tatum that the role of genes is to specify proteins. From 1946 to 1960 he led the Caltech Biology Division, rebuilding it to a powerhouse in molecular biology, and afterwards became a successful President of the University of Chicago. This is the first biography of a giant of genetics, written by two of the field's most distinguished contributors, Paul Berg and Maxine Singer.
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 | G2793 | Gold and Silver Staining: Techniques in Molecular Morphology | Immunogold silver staining is one of the most sensitive techniques for visualizing the location of antibodies and nucleotide probes. Contributors cover the latest developments and a range of applications from highly sensitive detection of antigens to single copy detection of DNA and RNA. This book presents descriptions that allow visualization of molecules never localized before. It also provides a selection of protocols with high sensitivity; presents techniques for cytochemistry, histochemistry, in situ hybridization, and immunoblotting applications; discusses the applications to cancer research; offers suggestions, tips, and guidelines to avoid unspecific precipitations; analyzes advantages and drawbacks of staining methods.
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 | Z702544 | Guide to Analysis of DNA Microarray Data, 2nd ed. | Written for those who don't have any special training in data analysis and statistics, this book begins where DNA array equipment leaves off: the image produced by the microarray. The text deals with the questions that arise starting at this point, providing an introduction to microarray technology, then moving on to image analysis, data analysis, cluster analysis, and beyond. This book offers practitioners reliable information using concrete examples and a clear style. This edition features entirely new chapters on Image analysis; Experiment design; Automated analysis, integrated analysis, and systems biology; Interpretation of results .
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 | Z702366 | Handbook of Molecular and Cellular Methods in Biology and Medicine, 2nd ed. | With step-by-step protocols, this edition presents up-to-date molecular and cellular methods and techniques, including additional methods for analysis of DNA-protein interactions by DNA footprinting and gel retardation assay, mRNA differential display, and microarray technology; Explains the principles behind each method; Includes helpful trouble-shooting guides. This edition includes: new protocols in all chapters, including alternative protocols; In vitro transcription methods; analysis of DNA sequences; new bioseparation techniques. New chapters cover: mRNA differential display; Inhibition of gene expression; In situ hybridization; Combinatorial techniques; Computational data mining methods applied to combinatorial chemistry libraries.
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 | Z704253 | Handbook of Plant Genome Mapping: Genetic and Physical Mapping | While the Human Genome Project has received much public attention, the deciphering of the plant genome, in particular in crop plants, is of great importance for science and industry in such fields as agriculture, biotechnology, and food. It is equally crucial for our understanding of the molecular basis of biodiversity. This is the first book on the market to cover this hot topic in such detail, and is set apart by its combination of genetic and physical mapping. Throughout, each chapter begins with an easy-to-read introduction, also making the book the first reference designed for non-specialists and newcomers, too.
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 | Z705624 | In Situ Hybridization Protocols 3rd ed. | This revised and updated edition of a recognized classic emphasizes tissue and cell in situ hybridization methods. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology–developmental gene detection, and expanded cell culture. Derivative techniques presented include identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols include step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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 | I8268 | In Situ Hybridization Protocols, 2nd ed. | This new edition contains basic and advanced in situ hybridization techniques, many not covered in the first edition. It includes protocols for in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscope level, in situ detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and use of DNA or RNA probes to detect expression in cells or tissue sections.
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| | I1768 | In Situ Hybridization: A Practical Approach | Step-by-step protocols are presented for the preparation of cloned DNA, RNA and oligonucleotide probes, with both radioactive and non-radioactive labels, and their hybridization to mRNA, viral DNA and chromosomal DNA.
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 | Z378240 | In Situ PCR Techniques | In situ hybridization combined with polymerase chain reaction permits detection and amplification of single-copy nucleic acid sequences in cell and tissue samples. It offers tremendous potential for diagnosis, as well as research in embryogenesis, differentiation, and disease processes. Topics address the difficulties of the combined techniques and help researchers develop less cumbersome, more reproducible protocols.
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 | I5906 | In Vitro Mutagenesis Protocols, 2nd ed. | The new edition includes many molecular biology techniques to study mutagenesis including the latest proteomics methods that are begin adapted by molecular biologist in their new studies of proteins. All of the newest technologies are covered including RT-PCR and gene shuffling.
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 | Z730718 | In Vitro Transcription and Translation Protocols 2nd ed. | This Second Edition provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration and of continuous-exchange devices to obtain high levels of in vitro protein production, the efficient in vitro production of integral membrane proteins, a protein category known to be extremely resistant to in vivo expression, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. Other chapters also discuss the efficient, high-throughput protein production using the wheat germ in vitro expression system and exogenous protein expression in xenopus oocytes. Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy and to follow the surge of drug resistance, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the use of in vitro transcription and translation to directly identify proteins in 2D-maps. Additional chapters investigate high-throughput in vitro expression of Bacillus anthracis antigens to identify vaccine candidates, the use of Xenopus laevis translation system to study the functional expression of type 1 rat GABA transporter, and the exploitation of wheat germ in vitro transcription and translation system for the production of highly purified proteins amenable to NMR studies.
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 | I2154 | Instant Notes in Molecular Biology, 2nd ed. | This second edition provides a structured approach to learning by covering all the important topics in a uniform, systematic format. Each topic begins with a summary of all essential facts, an ideal revision checklist, followed by detailed explanation and clear, simple diagrams. Topics covered include cells and macromolecules, protein structure, properties of nucleic acids, prokaryotic and eukaryotic chromosome structure, and DNA replication.
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 | Z702803 | Internet for Cell and Molecular Biologists, 2nd ed. | The second edition of this popular book explains in detail the expanding possibilities that the internet offers cell biologists, molecular biologists, and other bioscientists. All aspects of the internet are discussed from mailing lists and discussion groups to sequence search facilities, primer design software, genome databases, computational genomics and microarray analysis. The book is packed with essential information on the use, purpose and future potential of all relevant resources. This manual was designed to enable the bioscientist to make skilful use of computers and of the rich internet tool-set.
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 | Z373826 | Laboratory Guide to RNA | This guide provides detailed accounts of experimental procedures ranging from the isolation, analysis, and quantification of RNA from a variety of sources to large and small scale synthesis of RNA. Protocols such as RNA blotting, nuclease protection, and in situ hybridization are covered. A solid reference on the analysis of gene expression at the RNA level.
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 | Z718734 | Lateral DNA Transfer: Mechanisms and Consequences | This book is about mobile genes - the transfer of DNA between unrelated cells. It discusses the machinery of gene transfer and its wide-ranging biological and health consequences. Mobile DNA makes possible the development of antibiotic resistance in microbes, the conversion of harmless to pathogenic bacteria, and the triggering of cancerous growth in cells. It also contributes to human evolution. This well-illustrated volume contains an up-to-date account of a topic now seen as increasingly important, and will be invaluable for both working scientists and as a textbook for advanced courses.
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 | Z704466 | Lentivirus Gene Engineering Protocols | Authors detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. They demonstrate lentivirus vector production with methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit.
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 | L6663 | Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques | Despite the large impact of PCR, many applications remain within the confines of research and academic environment. Dr. Harris makes this technique accessible to researchers, physicians, and laboratory workers throughout the world. It provides practical details of the method, and technology transfer program that she developed. It serves as a guide for those with limited budgets, such as potential users in developing countries, those wishing to work abroad or in undergraduate labs. Specific applications are immediately useful to study of infectious diseases.
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 | M7682 | MHC: A Practical Approach, 2 Volume Set | The major histocompatibility complex is the subject of much research in the immunology field. There is a great deal of interest in MHC proteins and their function as antigen presenting molecules, and many immunology laboratories are investigating biochemical and genetic techniques to study these molecules. The identification of peptide transporter genes and the elution of peptides from MHC molecules illustrate how rapidly our understanding of the MHC locus has advanced. These volumes bring together the technologies, which make these advances possible. It explains in detail the process such as peptide translocation into the ER, the application of mass spectrometry to the analysis of peptides bound to MHC, and signal detection via MHC class II molecules, which lead to either activation or cell death.
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 | Z700401 | Manipulating the Mouse Embryo: A Laboratory Manual, 3rd ed. | Regarded as the "Bible" of mammalian embryo manipulation techniques, the third edition has been completely reorganized and rewritten. The result is a compilation of new, cutting-edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques.
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 | Z379743 | Manipulation and Expression of Recombinant DNA: A Laboratory Manual | The techniques used in basic molecular biology research, presented as a series of tested protocols for students. Experiments are modular, allowing the instructor maximum flexibility for adapting to course curricula. The “project” approach give students an overview of an entire series of processes. Covers all important techniques and protocols, including some immunological techniques. Each lab session includes an introduction to the theoretical aspects of new techniques, references, and a list of materials used.
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 | Z718408 | Mechanisms of Transcription | Complex macromolecular machinery is responsible for gene-specific and cell-type selective patterns of gene expression. How it works is being revealed by increasingly sophisticated molecular dissection of prokaryotic and eukaryotic cells. In this volume, sixty-seven of the world’s leaders in the study of gene transcription review the results and conclusions of their most recent studies. They cover events ranging from activation, through promoter recognition, repression, chromosome structure, chromatin remodeling, initiation and elongation, and the regulation of stopping and starting. Written with a broad audience of molecular biologists in mind, extensively illustrated and completed by a masterly overview from Richard Losick, this book is an up-to-date and accessible survey of a topic at the heart of molecular biology and biotechnology.
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 | Z700584 | Methods in Molecular Biology and Protein Chemistry: Cloning and Characterization of an Enterotoxin Subunit | This book is a guide to master current molecular biology and biochemistry techniques. It adopts a hands-on approach to encourage the reader to explore and experiment independently. The book is designed to support a course in which students participate in an ongoing research project, give a research presentation on a topic related to the project, and write a research article suitable for publication. It features a sequence of experiments, a mock exercise, and a supplementary Website.
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 | Z705543 | MicroRNA Protocols | MicroRNA Protocols provides diverse, novel, and useful descriptions of miRNAs in several species, including plants, worms, flies, fish, chicks, mice, and humans. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the identification of miRNAs. This goal of this volume is to stimulate the reader to explore diverse ways to understanding the mechanism in which miRNAs facilitate the molecular aspects of the biomedical research.
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 | Z700290 | Microarray Analysis | Microarrays enable one to analyze genes, proteins and other biological molecules on a genomic scale. This comprehensive and multidisciplinary text contains separate chapters devoted to surface chemistry, target and probe preparation, microarray manufacture and detection, and data analysis and modeling. Methodology, clean room technology, gene expression profiling, genetic screening and diagnostics, and novel technologies are also covered. It rapidly teaches the fundamentals of microarrays; describes protein microarrays and other rapidly advancing forefronts; builds a conceptual foundation for the entire field; presents commercial opportunities in microarray science; emphasizes the exciting clinical applications of microarrays.
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 | Z702315 | Microarray Bioinformatics | DNA microarrays have revolutionized molecular biology and are becoming a standard tool in the field. Dov Stekel's book is a comprehensive guide to the mathematics, statistics and computing required to use microarrays successfully. It covers all aspects of microarray bioinformatics, giving you the tools to design arrays and experiments, to analyze your data, and to share your results inside or outside your organization. There are chapters covering sequence databases, oligonucleotide design, experimental design, image processing, nomalization, identifying differentially expressed genes, clustering, classification and data standards. It is based on the successful course at Oxford University.
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 | Z702404 | Microarray Quality Control | Microarray technology is a powerful tool for measuring gene expression, but the multiple steps involved, from sample selection to data analysis, are susceptible to potentially costly errors. Without sound quality control, experimental microarrays may produce useless or, even worse, misleading results. From experimental design to data processing, analysis, and interpretation, the text's emphasis remains on practical advice for each stage of planning and running a microarray study. Chapters cover: Quality of biological samples; Quality of DNA; Hybridization protocols scanning; Data acquisition; Image analysis; Data analysis.
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 | Z701394 | Microarrays Methods and Applications: Nuts and Bolts | This book is a "how to" manual for the laboratory researcher who is using or plans to employ DNA or protein microarrays. It is packed with useful protocols for various microarray technologies; statistical experimental design concepts; micorarray image analysis tips; microarray data analysis recommendations; analysis of microarray automation and software; applications in many research fields including pharmacology.
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 | Z700312 | Microarrays for an Integrative Genomics | The growing use of DNA microarrays allows researchers to assess the expression of tens of thousands of genes at a time. This book provides an introduction to the use of DNA microarrays as an investigative tool for functional genomics. The presentation is appropriate for readers from biology or bioinformatics. After presenting a framework for the design of microarray-driven functional genomics experiments, the book discusses the foundations for analyzing microarray data sets, genomic data-mining, the creation of standardized nomenclature and data models, clinical applications of functional genomics research, and the future of functional genomics.
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 | M0941 | Molecular Biology Made Simple and Fun | The authors present a simple and amusing approach to molecular biology. Written primarily for the beginning science student, the reader will gain an understanding of the fundamentals and tools of molecular biology. This edition contains a new chapter on several discoveries since the first edition, such as the cloning of Dolly the sheep. Another new chapter helps readers to understand a research seminar in molecular biology. It explains common technical terms and procedures likely to be met during a presentation on current research in this area. Also the coverage of other areas such as the Human Genome Project has been updated.
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| Z704873 | Molecular Biology Made Simple and Fun, 3rd ed. | For those who expect to be part of what's happening in the 21st century, the impact of molecular genetics research should not be underestimated. The authors present a simple and fun approach to the topic of molecular biology. Written primarily for the science student, but suitable for the non-scientist, the reader will gain a solid understanding of the fundamentals and tools of molecular biology. The book also details how this rapidly advancing field has and will continue to have an impact on health, law, agriculture, biotechnology and our understanding of the origins of the species. The third edition includes three new chapters and thirty new illustrations. New topics include the latest information regarding RNA and a chemistry review section.
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 | M5313 | Molecular Biology Problem Solver: A Laboratory Guide | This is a unique resource that will strengthen your ability to troubleshoot experiments by providing a firm basis for understanding how things work and what can go wrong. The materials serve a framework on which to build strong analytical bench skills. Coverage includes working with hazardous materials, calculations and reagent preparation. Clarifying theory and practice, the focus is on the critical elements of techniques, reagents and procedures.
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 | Z702005 | Molecular Biology in Medicinal Chemistry | Molecular biology and genetic engineering have made a great impact on the methods and strategies of modern pharmaceutical and medicinal chemistry, transforming every step in the drug development process, from target finding to drug design and ADMET analysis. Even the synthesis and manufacture of pharmaceutically active compounds has benefited from novel approaches based on molecular biology tools. Topics include: Cellular assays in drug discovery; Transgenic models; Reporter gene assay systems; Stereoselective synthesis with recombinant enzymes; Nucleic acid drugs; Enantioseparation of chiral drugs; NMR-based drug discovery; Pharmacogenomics and toxicogenomics.
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| | Z730890 | Molecular Biology of Aging | Research into the causes of aging, and strategies to delay that process, have gained much ground and attention in recent years. This volume covers the major threads in the molecular genetics of aging, including genes that regulate aging, causes of aging, evolutionary theories of aging, and the relationship between diet and aging. Among specific topics covered are calorie restriction, mitochondria, sirtuins, telomeres, stem cells, and cancer. Each chapter is written by one or more leaders in the field, and the book presents the current status of this exciting research area and provides an invaluable source of information in a single volume.
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 | Z700274 | Molecular Biology of Drug Addiction | A review of the most relevant molecular, genetic, and behavioral approaches used to investigate the neurobiological basis of drug addiction. Latest findings on opioid, psychostimulant, cannabinoid, alcohol, and nicotine addiction are presented and provide insights into the genetic basis of drug addiction and new therapeutic perspectives these have opened. Authors describe the technology available to generate conditional knockout mice and show how these mice can reveal the molecular basis of opioid, psychostimulant, and cannabinoid addiction. They also review the different behavioral models available to evaluate the rewarding effects of drugs and analyze the genes involved in alcohol dependence.
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 | Z731420 | Molecular Biology of the Cell 5th ed. | For nearly a quarter century Molecular Biology of the Cell has been the leading cell biology textbook. This tradition continues with the new Fifth Edition, which has been completely revised and updated to describe our current, rapidly advancing understanding of cell biology. To list but a few examples, a large amount of new material is presented on epigenetics; stem cells; RNAi; comparative genomics; the latest cancer therapies; apoptosis (now its own separate chapter); and cell cycle control and the mechanics of M phase (now integrated into one chapter).
The hallmark features of Molecular Biology of the Cell have been retained, such as its consistent and comprehensive art program, clear concept headings, and succinct section summaries. Additionally, in response to extensive feedback from readers, the Fifth Edition now includes several new features.
It is now more portable. Chapters 1-20 are printed and Chapters 21-25, covering multicellular systems, are provided as pdf files on the free Media DVD-ROM which accompanies the book.* And for the first time, Molecular Biology of the Cell now contains end-of-chapter questions. These problems, written by John Wilson and Tim Hunt, emphasize a quantitative approach and the art of reasoning from experiments, and -they will help students review and extend their knowledge derived from reading the textbook. The Media DVD-ROM, which is packaged with every copy of the book, contains PowerPoint® presentations with all of the figures, tables and micrographs from the text (available as JPEGs too). Also included is the Media Player, which plays over 125 movies—animations, videos, and molecular models—all with voiceover narration. A new reader-friendly feature is the integration of media codes throughout the text that link directly to relevant videos and animations. The Media DVD-ROM holds the multicellular systems chapters (21-25) of the text as well.
By skillfully extracting the fundamental concepts from this enormous and ever-growing field, the authors tell the story of cell biology, and thereby create a coherent framework through which readers may approach and enjoy this subject that is so central to all of biology.
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 | M5940 | Molecular Biology of the Cell, 4th ed.(with CD-ROM) | This edition is a massive revision of the previous edition. It is the classic in-depth text reference in cell biology, yet puts the latest hot topics sensibly in context - including genomics, protein structure, array technology, stem cells and genetic diseases. It incorporates and emphasized new genomic data. It not only sets forth the current understanding of cell biology, but also explores the intriguing implications and possibilities of that which remains unknown. It contains over 1,600 illustrations, electron micrographs, and photographs, of which over 1,000 are originally conceived by the authors. “Cell Biology Interactive”, a CD-ROM, is packaged with every copy.
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 | Z701823 | Molecular Biology of the Gene, 5th ed. | This edition of Watson's classic textbook has been brought up to date, and incorporates insights very recently derived from genome sequencing in a variety of organisms. The book is a comprehensive survey of the fundamentals of molecular biology, from basic mechanisms to the elaborate role of gene regulation in embryonic development and evolution. The 21 chapters retain the distinctive features of the original work, including introductory chapters on the history of genetics and molecular biology and an emphasis throughout on the chemical underpinnings of molecular biology. By revealing the intellectual framework and experimental approaches that made new discoveries in the field possible, this edition highlights the significance of the molecular approach for all of biology.
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| | M8534 | Molecular Biology: Labfax | This book will serve as a convenient reference for molecular biologists and scientists in related areas. It is a detailed compendium of essential information on genotypes, reagents, enzymes, reaction conditions, cloning vectors and other information needed to plan and carry out molecular biology research.
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 | Z702056 | Molecular Cell Biology, 5th ed. | This is the new edition of a college level textbook on molecular cell biology. The emphasis is on experimental basis of current understandings. New experimental methodologies and concepts are introduced in this edition. Thirteen chapters cover such topics as biomembranes and cell architecture, cell integration in tissues, cell transport mechanisms, cellular energetics, molecular genetic techniques, transcriptional control of gene expression, and signaling at the cell surface.
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 | M8265 | Molecular Cloning: A Laboratory Manual, 3rd ed., Vols 1,2 and 3 | In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved. It includes 240 laboratory protocols in DNA science in which over 35% were created especially for this edition, along with coverage of bioinformatics and DNA microarrays.
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 | Z700762 | Molecular Infection Biology: Interactions Between Microorganisms and Cells | This volume focuses on molecular methods and principles of prokaryotic and eukaryotic pathogens. The editors present the molecular and cellular aspects by focusing on the interactions between pathogenic microorganisms and their hosts. This book leads into discussions of how microbial “weapons,” pathogenicity factors, protein secretion machines, and surface variation systems work, while presenting the molecular and genetic methods that are being used by scientists for their discovery and analysis. Infectious diseases are discussed in light of the newly formed research areas.
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| | Z359734 | Molecular Methods for Virus Detection | This book provides both reviews of individual methods and instructions for detecting viral and mycobacterial nucleic acid sequences in clinical specimens. State-of-the-art PCR methods are presented, as well as protocols for 3SR, NASBA, LCR, SDA and LAT. Quality control information for research and clinical laboratories is included.
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 | Z700967 | Molecular and Cellular Biology of Filamentous Fungi, A Practical Approach | Identification and characterization of genes from filamentous fungi has become easier. The challenge is to determine the functions of genes and how they contribute to the biology of fungi. To do this a variety of different experimental techniques will be necessary, ranging from classical genetic analysis and mutant production, right through to cell biology, biochemistry and immunological methods. This book gives guidance on the full range of these methods. Authors describe laboratory procedures that have been effective for the investigation of a variety of fungal species. They give background information, technical tips and experimental protocols.
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 | M6060 | Molecular and Cellular Glycobiology | This book covers the recent progress in understanding the biological roles of carbohydrates. Emphasis is particularly placed on biological significance in immune cells, neural cells and cytoplasmic and nuclear proteins. It updates the knowledge of diseases caused by anomalies of carbohydrate biosynthesis and the animal models used to investigate the physiological roles of carbohydrates including carbohydrates attached to lipids and attempts to develop therapeutic agents based on carbohydrates.
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 | Z718793 | Mouse Phenotypes: A Handbook of Mutation Analysis | The generation of mutant mice raises many questions about the best means of phenotypic analysis, breeding, and maintenance. The answers are now available from two experts with a wealth of detailed knowledge never previously assembled in one volume. Informal and highly practical, this handbook provides step-by-step methods for troubleshooting experiments, from the basics of gene targeting through the analysis of postnatal effects. All investigators using the laboratory mouse in genetic studies designed to understand mammalian biology will benefit from the authors' experience and advice.
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 | Z379700 | Nitric Oxide Protocols | A methods manual for studying all aspects of NO and its metabolism. Readily reproducible techniques form a core of effective experimental methods for researchers new to the field and serve as a ready reference for experienced investigators. Cloning and expression of NO synthase isoforms, measurement of NO synthase activity, direct and indirect quantitation of NO, and the role of NO in DNA damage and apoptosis are all covered. In addition to stepwise instructions, each protocol also summarizes pros and cons and describes pitfalls and troubleshooting.
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 | Z705209 | Nuclear Reprogramming: Methods and Protocols | A collection of reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and verification of surface marker expression and the differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, and the study of DNA methylation changes.
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 | Z700231 | Nucleases: Molecular Biology and Applications | This resource on nucleases introduces the properties and biological roles along with the basis of their application. Covering the structure and biochemical properties, this text guides one to understand how these enzymes can be exploited to make new products for the diagnosis and treatment of disease. Molecular cloning, made possible by the evolving understanding and application of nucleases, may well lead to the identification and characterization of genes responsible for diseases and their possible alleviation by gene therapy and the development of designer drugs. Chapter topics include ribonucleases, restriction endonucleases, topoisomerases, sugar non-specific nucleases, and molecules that interact with nucleases.
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 | Z378380 | Nucleic Acid Amplification Technologies: Application to Disease Diagnosis | | pricing |
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 | Z371963 | Nucleic Acid Analysis: Principles and Bioapplications | Thorough coverage of methods and strategies in two sections. The methods section details hybridization assays, cloning, labeling probes, and amplifying probe signals. The applications section includes discussions of forensic applications, screening for genetic diseases, infectious disease diagnosis, and applications to food technology and environmental science.
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 | N8029 | Nucleic Acid Protocols Handbook | A comprehensive volume of molecular biology methods ranging from DNA extraction to gene localization in situ. The 120 techniques cited list all necessary materials and reagents, step-by-step instruction, pitfalls to avoid, troubleshooting tips, alternate methods, and reasons for certain steps. All key elements contributing significantly to success or failure in the lab. It is a collection of all classic and cutting-edge techniques for isolation, analysis, and manipulation of nucleic acids.
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 | N6285 | Nucleic Acid Structure and Recognition | This book provides a view of the molecular structures of DNA and RNA and how they are recognized by small molecules and proteins. Source material is provided, including information on relevant web sites and computer programs. The major methods of structural investigation for nucleic acids, x-ray crystallography, NMR, and molecular modelling, are reviewed and their scope and limitations discussed. Also covered are the conformational features of nucleic acid building blocks. Both covalent and non-covalent nucleic acid interactions with small molecules are described, with the emphasis on recognition principles and sequence specific gene recognition.
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 | Z703605 | Oligonucleotide Synthesis: Methods and Applications | A collection of powerful techniques for oligonucleotide synthesis and for use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
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 | O8006 | Oxford Handbook of Nucleic Acid Structure | A comprehensive reference on all aspects of nucleic acid structure, with particular emphasis on results from x-ray crystallography and NMR studies. It describes in detail the variety of structural types discovered to date. It is set out in a systematic manner, progressing through the polymorphs of double helical DNA through to the higher order organizations of triplexes, quadruplexes and junctions, then to RNA structures in their various degrees of complexity.
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 | O1389 | Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols | This book presents cutting-edge molecular and ultrastructure techniques for investigating the pathophysiology of oxidative stress. Described in step-by-step detail, these readily reproducible methods use the latest in immunodetection, x-ray diffraction, confocal microscopy, in situ hybridization, chemiluminescence, and cytofluorescence. Additional techniques take advantage of electron paramagnetic resonance, advanced glycation endproducts, Western blotting analysis, transcription factors, p47 phox translocation, and microsphere delivery systems.
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 | Z730300 | PCR 2nd ed. | A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.
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 | P4609 | PCR Applications: Protocols for Functional Genomics | From ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences, PCR Applications examines the latest developments in this field. It includes statistical refinement of primer design parameters, techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology.
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 | P8117 | PCR Cloning Protocols, 2nd ed. | This edition update and expands Bruce White′s best-selling “PCR Protocols” (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long PCR and GC-rich template amplification. Powerful applications of PCR in library construction and sublibrary generation and screening are presented.
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 | P8867 | PCR Mutation Detection Protocols | This book provides biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included.
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 | Z731110 | PCR Primer Design | In this book authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome scale experiments and for small scale individual PCR amplifications. This volume will be useful for organizations perfomring whole genome studies, companies designing instruments that utilize PCR, and individual scientists who routinely use PCR in their research.
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 | Z701270 | PCR Primer: A Laboratory Manual, 2nd ed. | This second edition of a much praised and widely used manual has been entirely revised and updated. Each technique is presented with extensive background information, advice, and troubleshooting. All current applications of PCR are covered in protocols that have the hallmark reliability of the previous edition.
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 | Z718874 | PCR Primer: A Laboratory Manual, Second Edition | This second edition of a much - praised and widely used manual has been entirely revised and updated. Each technique is presented with extensive background information, advice, and troubleshooting. All current applications of PCR are covered, in protocols that have the hallmark reliability of the previous edition.
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 | Z364118 | PCR Primer: A Laboratory Manual | This manual covers a wide range of PCR and other amplification techniques from the basic aspects to more advanced levels. Each protocol includes analytical methods and a troubleshooting section.
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 | Z369918 | PCR Protocols for Emerging Infectious Diseases | The spectres of completely new infectious diseases and multiple drug-resistant pathogens are frightening reminders of our frailty. Modern PCR techniques provide the best methods for identifying the causes. This book presents 12 detailed protocols that can serve as models for development of new protocols when they are needed.
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 | Z701351 | PCR Protocols, 2nd ed. | This edition has updated and expanded the number of protocols to take advantage of the newest technologies. These methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each protocol is described in step-by-step detail and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding pitfalls, and a discussion of the interpretation and use of results.
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| | P8177 | PCR Protocols: A Guide to Methods and Applications | The polymerase chain reaction (PCR) is a powerful new molecular biological method with widespread applications in genetics and disease diagnosis. This manual details current PCR methods.
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 | Z373818 | PCR Sequencing Protocols | This book offers a comprehensive collection of the most reliable protocols for PCR Sequencing. Informative explanations of why certain steps are necessary and extensive tips that help make the difference between success and failure are also included. This book will offer time-saving techniques for even highly skilled researchers.
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| | Z364452 | PCR Strategies | A compilation of short articles written by PCR experts specifically for this volume. Practical information and advice includes background on key PCR concepts, analysis of PCR products, special applications, and alternatives to PCR for gene amplification.
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| | P5855 | PCR Technique: Quantitative PCR | This volume in the “BioTechniques Update Series” collects a number of important papers focused on various aspects of quantitative PCR that first appeared in BioTechniques from 1991 to 1996. This period of time witnessed the introduction, rapid development and numerous improvements of this laboratory method. These papers, most of which were reviewed and updated by the original authors, present novel methods to quantitate PCR products, improvements and modifications of previously published methods, and applications of quantitative PCR to several clinical situationa.
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| | Z357499 | PCR Technology, Current Innovations | | pricing |
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 | Z704318 | PCR Technology: Current Innovations, 2nd ed. | This book is a fundamental reference for scientists new to PCR technology and a source of up-to-date applications for those familiar with the method. It provides theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. The book begins with discussions of the preparation of PCR experiments, followed by examples of analytical PCR divided into qualitative and quantitative applications. The final section explores preparative methods addressing DNA generation for further analysis and in vitro evolution. Featuring detailed protocols, this volume contains critical information for practitioners in a wide variety of fields, including forensics, molecular biology research, clinical DNA diagnostics, botany, and paleontology.
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 | Z705489 | PCR Troubleshooting: the Essential Guide | A unique PCR troubleshooting guide that is an essential companion for anyone who uses the polymerase chain reaction technique. Aimed at a reader with some experience in PCR the book discusses the many and varied problems encountered with PCR together with tips, advice and procedures to obviate rather than overcome the PCR problems. Written in the language of the laboratory with a little humour and a down-to-earth approach, the book is easy to read and understand. If you fail at PCR, consult this book! The advice in these pages is invaluable and is the sort of advice that is not usually found elsewhere.
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 | Z379603 | PCR in Bioanalysis: Methods in Molecular Biology, Vol. 92 | Marking the transformation of PCR from a purely research technique to an analytical tool, this volume details 22 cutting-edge protocols for molecular diagnosis of specific diseases. Coverage extends to infectious diseases, genetic syndromes, and cancer in humans, animals and plants. Diagnose such diseases as hepatitis C, tuberculosis, chlamydia and trichomonad infections. Animal tests include those for a bovine HIV-like virus, rodent parvoviruses, and Ross River virus. There are also general protocols for selective UV radiation fractionation (SURF) and for detecting circulating cancer cells. Overall, a concise but thorough introduction to practical diagnostic PCR.
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 | Z702374 | PCR/RT-PCR in situ Light and Electron Microscopy | Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. This book covers methods of in situ PCR and reverse transcription PCR (RT-PCR), two approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopy levels. It provides theoretical consideration, as well as practical approaches to in situ PCR. Authors provide detailed protocols including the preparation of tissue samples, the rationale for the design of primers and revelation. They emphasize the need for appropriate controls to meet the requirements of in situ PCR and RT-PCR specificity.
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| | P7186 | PCR: A Practical Approach | This book covers gene analysis at the level of restriction fragment polymorphism, point mutation, and sequence analysis. Methods are given for using different source materials: cloned DNA, genomic DNA, RNA, nucleic acid from archive material, and PCR products themselves.
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| | Z719285 | PCR hard cover | PCR: Methods Express describes the latest PCR-based methodologies and approaches and provides the most up-to-date practical advice on how to tackle a broad range of biological problems using these techniques, including:
• Quantitative RT-PCR
• Identification of genomic micro-deletions and duplications
• DNA methylation analysis
• DNA typing for forensic genetics
• Rapid generation of gene targeting constructs
• Pre-implantation genetic disease diagnosis
• Construction of long DNA molecules from multiple fragments
• Identification of low level mutations in tumors
PCR: Methods Express is a comprehensive research guide; every chapter discusses the merits and limitations of various approaches and then provides selected tried-and-tested protocols with useful hints and tips for success, along with strategies for optimization.
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| | Z719277 | PCR soft cover | PCR: Methods Express describes the latest PCR-based methodologies and approaches and provides the most up-to-date practical advice on how to tackle a broad range of biological problems using these techniques, including:
• Quantitative RT-PCR
• Identification of genomic micro-deletions and duplications
• DNA methylation analysis
• DNA typing for forensic genetics
• Rapid generation of gene targeting constructs
• Pre-implantation genetic disease diagnosis
• Construction of long DNA molecules from multiple fragments
• Identification of low level mutations in tumors
PCR: Methods Express is a comprehensive research guide; every chapter discusses the merits and limitations of various approaches and then provides selected tried-and-tested protocols with useful hints and tips for success, along with strategies for optimization.
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 | Z378399 | PCR 3: PCR In Situ Hybridization | An extension of a popular series, this volume is subtitled “PCR In Situ Hybridization.” Coverage emphasizes the power of the combined techniques to detect and amplify rare nucleic acid sequences in biological samples. Step-by-step details of key procedures, advice, and troubleshooting tips improve reliability and help prove the enormous potential for research and diagnosis.
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 | Z705608 | PRINS and In Situ PCR Protocols, 2nd ed. | This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. The authors describe in detail various applications of PRINS in human (detection of gene deletions in cancer detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus, and for use in combination with microdissection.
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| | Z373931 | PRINS and In Situ PCR Protocols: Methods in Molecular Biology, Vol. 71 | This new, cutting edge publication demonstrates clearly how oligonucleotide primers may be successfully used to detect and amplify, or extend, complementary sequences ::FIin situ. It provides methods for rapid identification and characterization of chromosomal cDNA sequences, viral genomes, and rare messenger RNAs in cells.
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 | P5861 | Phage Display: A Laboratory Manual | Phage display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage display technology is challenging and the aim of this manual is to provide comprehensive instruction in its theory and application.
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| | Z730815 | Phage and the Origins of Molecular Biology, the Centennial Edition | This hugely influential book, published in 1966 as a 60th birthday tribute to Max Delbrück, is now republished as The Centennial Edition. On first publication, the book was hailed as “[introducing] into the literature of science, for the first time, a self–conscious historical element in which the participants in scientific discovery engage in writing their own chronicle. As such, it is an important document in the history of biology...” (Journal of History of Biology). And in another review it was described as “required reading for every student of experimental biology...[who] will sense the smell and rattle of the laboratory” (Bioscience). The book was a formative influence on many of today’s leading scientists.
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 | Z704385 | Pharmacogenomics: Methods and Protocols | Experts provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromarography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions.
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 | Z701874 | Plant Functional Genomics | Leading plant scientists describe the most commonly used methods for investigating plant gene function in a wide variety of plants, during plant pathogen interactions, and even in algae. These readily reproducible protocols include computational, molecular, and genetic methodologies designed for both general and specific problems. The reader will learn about powerful computational and statistical tools to help predict gene function either on the basis of comparative genomics, or from the analysis of complex genome sequences. Numerous loss-of-function and gain-of-function techniques for discovering gene function are presented in step-by-step detail
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 | P2231 | Positional Cloning by Exon Trapping, cDNA Selection | This book covers the practical and theoretical information on broadly applicable techniques to identify and characterize candidate genes that may be responsible for human diseases. In order to understand the function of these genes, it is essential to link the information on the phenotype, localized to specific regions of the genome by genetic analysis techniques.
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 | P7109 | Post-genome Informatics | New DNA and protein chip technologies rapidly produce functional data about genes. But there is a huge gap between information at the molecular level and at the level of integrated biological systems. This book addresses this gap. It is the analysis of biological functions in terms of the network of interacting molecules and genes with the aim of understanding how a biological system is organized from its individual building blocks. It contains computational technologies relevant to molecular sequence analysis.
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 | P4859 | Practical in situ Hybridization | A detailed practical guide for laboratory use aimed at those who wish to use efficient and reliable protocols in their work and at researchers checking the validity and interpretation of published data. These molecular cytogenetic methods can be used to characterize and locate nucleic acid sequences within the cell. It is presented in a user-friendly, open format for easy access to information with an extensive troubleshooting guide.
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 | Z700630 | Principles of Genome Analysis and Genomics, 3rd ed. | With the first draft of the human genome project in the public domain and full analyses of model genomes now available, the subject matter of 'Principles of Genome Analysis and Genomics' is even hotter now than when the first two editions were published. In this edition of this very practical guide to the different techniques and theory behind genomes and genome analysis, the authors provide a fresh look at this topic. In the light of recent advancements in the field, the authors have completely revised and rewritten many parts of the new edition with the addition of five new chapters.
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| | Z371947 | Quantitation of mRNA by Polymerase Chain Reaction | A concise guide to non-radioactive PCR techniques for quantifying nucleic acids in biological and clinical samples. Detection methods include HPLC and biotin- and digoxigenin-based protocols. This is a hands-on laboratory manual for beginners and professionals alike.
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 | R5152 | RNA Editing | This book devotes a chapter to each of the major types of this form of RNA processing. Each chapter offers fundamental principles, as well as up to date information on recent advances. Numerous examples of RNAs known to be edited are provided throughout and the book highlights the mechanistic diversity found among the various types of RNA editing. RNAs are cleaved, ligated, and deaminated on their way to maturation, and in some cases, their sequence is even altered in the brief moment when RNA polymerase stalls. The chemical reactions that allow RNA editing, and the RNA and proteins that direct the process are all described.
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 | Z702552 | RNA Interference (RNAi): Nuts & Bolts of RNAi Technology | This book offers protocols and advice for the use of RNA interference for knocking down expression of target genes in a number of different eukaryotes. Varied applications in mammalian systems are explored in depth by authors currently working at the leading edge of RNA intererence methodologies. Chapters include: Design, synthesis and preparation of synthetic RNAi; Expression of RNAi from recombinant DNA and viral vectors; High-throughput RNAi applications; RNAi in mammalian systems.
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 | Z703346 | RNA Interference in Practice | This hand-on guide to RNA interference makes the power of targeted gene silencing available to any laboratory with a basic equipment for the handling of nucleic acids. In easy to follow step-by-step protocols you will learn: * How RNAi works in plants, flies and mammals * How to design the most efficient RNAi constructs * How to achieve transient, stable and conditional RNAi in cell cultures * How to determine the efficiency of an RNAi experiment * How to use RNAi for gene therapy. All protocols have been thoroughly tested in the author's laboratory. Examples of successful experiments and troubleshooting hints are provided to help in establishing your own successful RNAi experiments.
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 | Z702730 | RNA Interference, Editing, and Modification: Methods and Protocols | A comprehensive collection of cutting-edge methods for elucidating the function of new genes and altering gene expression. These readily reproducible techniques can be used either in transient and stable gene splicing applied to worms, flies, trypanosomes, mammals, and plants, or in studying RNA editing mechanisms in a wide range of organisms, including systems that involve the conversion of one base to another and insertion/deletion editing. Topics of interest include stable and transient RNA interference, gene silencing, RNA editing, bioinformatics, small noncoding RNAs, and RNomics.
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 | R1025 | RNA Isolation and Characterization Protocols | Innovative biological investigators offer a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The book also includes protocols for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility.
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 | Z704490 | RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 3rd ed. | This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. This book includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.
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 | Z700207 | RNA Motifs and Regulatory Elements, 2nd ed. | This edition alerts the reader to the importance of regulatory RNA elements for the different areas of cellular life. The computational and experimental methods and tools to search for new interesting regulatory RNA structures are explained and compared. The knowledge on regulatory RNA structures and elements already available is concisely summarized. In addition, interesting RNA elements are analyzed in detail regarding their dynamics, regulation, and as a dominant topic of current research in molecular biology, including areas such as RNA mediated regulation of gene-expression, DNA/RNA chip data, and ribozymes, splicing, or telomerases in aging.
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| | Z357529 | RNA Processing: A Practical Approach Volume I | Detailed, practical guidance in this area of intense research activityu is now available in theses two volumes. Protocols from the leading laboratories are clearly outlined with explanations of the key steps required for a successful experimental investigation.
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| | Z357537 | RNA Processing: A Practical Approach Volume II | Detailed, practical guidance in this area of intense research activityu is now available in theses two volumes. Protocols from the leading laboratories are clearly outlined with explanations of the key steps required for a successful experimental investigation.
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 | Z704156 | RNA Silencing: Methods and Protocols | A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. Protocols follow the Methods in Molecular Biology series format.
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 | Z718300 | RNA Structure and Function | The striking functional versatility of RNA is attributable to its remarkable capacity for conformational change. Current models of RNA synthesis, its maturation, translation, and degradation are all based on specific structures and there is growing appreciation of the functional importance of interactions between RNA and RNA-binding proteins. This book identifies the major intellectual and technical advances in understanding the part played by structure in how RNA works. It encompasses the experimental approaches used to define RNA structure and its influence on RNA functions such as transcription termination, catalysis, recoding, and translational control. Investigators with these interests and students of molecular biology will find this volume a thoroughly up-to-date, provocative survey of a rapidly advancing field.
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 | Z359491 | RNA World, 2nd ed. | This new edition has been revised, updated, and extended to offer a completely current perspective on the modern world of RNA and the light it sheds on a prebiotic era perhaps dominated by this extraordinarily versatile molecule. Essential reading for everyone interested in the biology of nucleic acids.
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 | Z704377 | RNA World, 3rd ed. | Recent studies on the activities of RNA in the cell have revolutionized our understanding of the many roles played by this molecule. The first two editions of The RNA World (1993, 1999) shed light on the pre–biotic era dominated by this versatile molecule, and provided an overview of the state of RNA research at the time. The new third edition updates this perspective, describing the vast array of newly discovered roles for RNA in the modern world. The updated original chapters are supplemented with new chapters on RNA–protein complexes, snRNPs and snoRNPs, telomerase RNA, RNAi, microRNAs, noncoding RNA, and many other subjects. This book is essential reading on the biology of nucleic acids and gene regulation.
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 | Z701130 | RNAi: A Guide to Gene Silencing Hardcover | The use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific, remarkably potent, and acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and cheaper way of mimicking the effects of mutations in both cell cultures and in living animals. This book presents the principles of RNA interference and reliable protocols for its laboratory use in C. elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more.
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 | R5277 | RT-PCR Protocols | A panel of molecular biologists and clinical researchers describe their novel, useful, and interesting RT-PCR applications. One will find reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For someone seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering
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 | Z730319 | Real-Time PCR | With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'.
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 | R9261 | Recombinant DNA, 2nd Edition | The application of recombinant DNA techniques to biology is bringing about a revolution in our understanding of living organisms. The aim of this edition is to show how recombinant DNA techniques have led to the explosion in our knowledge of fundamental processes.
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 | Z702854 | Recombinant Gene Expression:Reviews and Protocols, 2nd ed. | In this edition, the editors have added critical reviews to a collection of protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of non-protein molecules. The protocols offer new host-vector systems, such as Anarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding.
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 | Z375586 | Recombinant Protein Protocols: Methods in Molecular Biology, Vol. 63 | Offers beginning and experienced researchers a firm foundation in the detection and isolation of a specific gene's recombinant protein. Practical and detailed information indispensable to successful large-scale production of defined gene products.
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| | Z730734 | Regulatory RNAs Paperback | Regulatory RNAs” was the theme of the 71st annual Cold Spring Harbor Symposium on Quantitative Biology, where scientists from around the world presented the latest advances in the biology of RNAs, including RNA interference, transcriptional and translational control, RNA editing, and the role of RNAs in biological circuits and epigenetic events. Investigators discussed the latest technologies aimed at large-scale characterization of RNAs, as well as the roles of small RNAs in development and cancer. This volume is a timely survey of this important new area of molecular biology.
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 | Z705632 | Reverse Transcriptase Inhibitors in HIV/AIDS Therapy | This is a survey of all aspects of the reverse transcriptase inhibitors (RTIs) used to treat HIV/AIDS, including drug discovery, pharmacology, development of drug resistance, toxicity, and prevention of mother-to-child transmission of HIV/AIDS. The authors synthesize our current understanding of the role of reverse transcriptase in the viral life cycle, describe the discovery and development of eight nucleoside and nucleotide analogs that represent milestones in treatment history, and thoroughly discuss the question of toxicity and resistance to this class of drugs. They also address three non-nucleoside RTIs and their pharmacokinetics and comparative clinical efficacy, new RTIs currently under development, and the impact of approved agents on treatment, in general, and on vertical transmission in the developing world.
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 | Z702331 | Ribozymes and siRNA Protocols, 2nd ed. | In this edition, hands-on experts take advantage of the latest advances in ribozyme, DNAzyme, and RNA interference technologies to describe in detail the successful methods available for gene inactivation in vitro and in vivo. Their optimized techniques employ hairpin ribozymes, DNAzymes, hammerhead ribozymes and derivatives, group I intron ribozymes, Rnase P ribozymes, and siRNAs, as well as general methods for RNA structure analysis, delivery of oligonucleotides, and gene therapy. Also provided are novel methods for identifying accessible cellular mRNA sites; group I intron and RNAse P ribozymes protocols for effective design, selection, and therapeutic applications; and the latest RNAi methods for sequencing-specific gene silencing in a wide variety of organisms.
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 | Z375551 | Sequence Data Analysis Guidebook: Methods in Molecular Biology, Vol. 70 | Leading researchers demonstrate how to examine the data produced by modern automated sequencing, how to assess quality, how to delete extraneous data and how to align sequence fragments using the most popular sequence analysis software packages available. Procedures for comparing newly derived sequences with the massive libraries of sequence databases are fully covered along with techniques for performing restriction analysis on, and making detailed analyses of, the expressed proteins.
•Includes complete tutorials for the leading sequence analysis software packages.
•Shows how to create detailed graphic representations of restriction sites.
•Demonstrates how to produce complex, publication-quality figures.
•Offers many protocols by the original developers, with input from experienced users.
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 | S8441 | Short Protocols in Molecular Biology, 5th ed., 2 Volume set | The new edition expanded to 2 volumes provides condensed descriptions of more than 700 methods compiled from Current Protocols in Molecular Biology. The books are specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research. Includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell populations.
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 | Z700339 | Single Nucleotide Polymorphisms: Methods and Protocols | This is a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
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 | Z731382 | Single-Molecule Techniques; A Laboratory Manual | As molecular and cellular biologists move toward nano–techniques for performing experiments on single molecules rather than on populations of molecules, a comprehensive manual on how (and why) to carry out such experiments is needed. Single-Molecule Techniques: A Laboratory Manual fills this requirement—it is the first to take researchers who know nothing about single–molecule analyses to the point where they can successfully design and execute appropriate experiments. Geared toward research scientists in structural and molecular biology, biochemistry, and biophysics, the manual will be useful to all who are interested in observing, manipulating, and elucidating the molecular mechanisms and discrete properties of macromolecules. Techniques range from in vivo and in vitro fluorescent–based methods to the use of atomic force microscopy, optical and magnetic tweezers, and nanopores. The book is edited by Paul R. Selvin and Taekjip Ha, two pioneers in the field of experimental biophysics who have made significant contributions to the development and application of single–molecule technologies.
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 | Z705047 | Synthetic Nucleic Acids as Inhibitors of Gene Expression: Mechanisms, Applications and Therapeutic Implications | Synthetic nucleic acids are at the cutting-edge of molecular strategies that target genes of interest in a specific, efficient and cost-effective manner. These tools are now used as gene-silencing agents in vitro and as novel therapeutics in human disease. This book brings together reviews by leaders in the fields of ribozymes, RNA interference, DNAzymes and oligonucleotide decoys, with emphasis on biochemistry and translational research. The chapters on ribozymes include discussions of ribozyme subtypes, structure-function aspects, and therapeutic applications. This is followed by a comprehensive discussion of DNAzymes, their various subtypes, kinetics and biochemistry, and applications in target validation. Finally, the mechanisms and multiple therapeutic applications of double-stranded oligonucleotide decoys are described.
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 | Z719129 | Telomeres, 2nd ed. | An up-to-date survey of the current exciting state of telomere biology. Telomeres, specialized structures found at the ends of chromosomes, are essential for maintaining the integrity of chromosomes and their faithful duplication during cell division. Chapters in this volume cover telomere structure and function in a range of organisms, focusing on how they are maintained, their roles in cell division and gene expression, and how deficiencies in these structures contribute to cancers and other diseases and even aging.
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| | Z719331 | The Dog and its Genome | Dogs of different breeds can range remarkably in size, shape, and behavior, and yet they all carry essentially the same genome, making them a particularly fascinating model for genome plasticity. The recent release of the complete sequence of the dog genome provides an exciting new context in which to consider such variation. Twenty-five chapters written by experts in the field include various aspects of morphological and behavioral variation in dogs, their origins and domestication, and their unique value as a model system for many common but complex human diseases such as diabetes and cancer.
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 | Z718718 | The Ribosome | This is the first book to contain the newly published findings on the structure of the ribosome and discuss their meaning for our understanding of how proteins are made and processed inside the cell. With over 60 contributions from the world’s most innovative ribosome biology laboratories, this is the latest volume in the annual series that for over 60 years has provided analysis and interpretation of the most interesting problems in biology.
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 | Z718726 | The Ribosome | This is the first book to contain the newly published findings on the structure of the ribosome and discuss their meaning for our understanding of how proteins are made and processed inside the cell. With over 60 contributions from the world’s most innovative ribosome biology laboratories, this is the latest volume in the annual series that for over 60 years has provided analysis and interpretation of the most interesting problems in biology.
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 | T8698 | Transcription Factors | Transcription factors are important in regulating gene expression, and their analysis is of paramount interest to molecular biologists studing this area. This book looks at the basic machinery of the cell involved in transcription in eukaryotes, the factors involved in transcription and progresses to look at the regulatory systems which control this machinery both within the cell and also in the wider systems of the mammalian organism. Features include comprehensive review of an increasingly important subject area, a unique collection of all recent work, and covers both transcription factors and their control, and also both normal and disease states
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 | Z357553 | Transcription Factors: A Practical Approach | Transcription factors are proteins that interact with specific DNA sequences to enable transcription to occur. This edition of a popular hands-on guide includes extensive updates and four new chapters, making it an ideal resource for standard techniques.
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 | T7442 | Transcription Regulation in Prokaryotes | This provides a clear and concise summary of the flow of information from genes to proteins in simple prokaryotic cells. While most books cover transcription in a single chapter with a strong emphasis on eukaryotic transcription, this text is devoted to prokaryotic transcription and is perfect for molecular biology, microbiology, and technology courses. It includes a comparative chapter for parallel and divergent mechanisms in prokaryotic and eukaryotic transcription regulation
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 | Z718785 | Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniques | In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Transcriptional Regulation in Eukaryotes answers that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene and the biochemistry of a new transcription factor.
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 | T0698 | Transgenesis Techniques: Principles and Protocols, 2nd ed. | This update presents techniques for generation and analysis of transgenic animals, with emphasis on manipulation of the mammalian genome. It concentrates on creation and maintenance of genetically modified murine strains, providing access to the germline by conventional pronuclear injection, and by retroviral and adenoviral infection. Emphasis is given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for constitutive and conditional gene targeting. Chapters include techniques for cryopreservation of male and female germlines and for generation of transgenic sheep by nuclear transfer.
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 | Z702935 | Trinucleotide Repeat Protocols | The protocols cover a variety of targets, ranging from DNA and RNA to proteins and whole animals, and focus not only on causal genes, but also on their affected products, such as transcription factors, neurotransmitter receptors, proteasomes, and mitochondria/oxidation damage. Experimental systems employed include E. coli, yeast, C. elegans, and mouse. Authors utilize a wide range of techniques, including gel electrophoresis, quantitative RT-PCR, immunological analysis, antibody usage and its applications, receptor assays using radioisotope handling, gene delivery by virus, brain cell and organotypic cultures, gender dependency, and neuron structure analysis.
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| | Z379891 | Vectors, Cloning Applications: Essential Techniques Series | A quick reference to the basic cloning procedures that must be mastered before DNA characterization or manipulation can be attempted. Includes in vitro transcription, cloning of very large DNA fragments for library construction, cloning of PCR products, and selected downstream manipulation techniques.
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 | Z719196 | Whole Genome Amplification: Methods Express Series Hardcover | Using Whole Genome Amplification (WGA) methods, it is possible to create microgram quantities of DNA starting with as little as one nanogram of genomic DNA and in some cases even a single eukaryotic or bacterial cell. The implementation of such WGA methods provides an ample supply of DNA for large-scale genetic studies. This title provides a comprehensive overview of the field and will be welcomed by all researchers looking to take advantage of the latest developments.
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| Z719218 | Whole Genome Amplification: Methods Express Series Softcover | Using Whole Genome Amplification (WGA) methods, it is possible to create microgram quantities of DNA starting with as little as one nanogram of genomic DNA and in some cases even a single eukaryotic or bacterial cell. The implementation of such WGA methods provides an ample supply of DNA for large-scale genetic studies. This title provides a comprehensive overview of the field and will be welcomed by all researchers looking to take advantage of the latest developments.
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 | Y4002 | Yeast Hybrid Technologies | Two-hybrid system provides a novel way of detecting protein-protein interactions through a powerful genetic approach. This approach has been well adapted to various other purposes, such as detecting protein-DNA interactions (one hybrid) and RNA-protein interactions or multiple protein interactions (three-hybrid). It provides detailed laboratory protocols, basic technology, variations, special applications, and troubleshooting suggestions. There is also useful information on in vitro protein interaction detection methods, and both bacterial and mammalian two-hybrid systems.
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 | Z378062 | Yeast Two-Hybrid System | This volume, part of the Advances in Molecular Biology series, presents detailed protocols and trouble-shooting advice on the yeast two-hybrid system, one of the most powerful and versatile methods for characterizing a protein's functions. Topics include: characterizing hormone/receptor complexes, identifying peptide ligands, how to identify mutations that disrupt an interaction, how to construct an activation domain hybrid library, and how to dissect the cell cycle and other complex genetic networks.
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 | Z702382 | mRNA Processing and Metabolism | A collection of reproducible techniques for the study of mRNA processing and metabolism. These techniques range from cotranscriptional processing events that occur while the mNA is engaged with elongating RNA polymerase II, to in vivo and in vitro splicing and its biochemical analysis, and alternative splicing. Additional methods cover mRNA export, the recovery and analysis of mRNP complexes, cytoplasmic translation, mRNA degradation in vivo and in vitro, and the controversial concept of nuclear translation. Each protocol is described in detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls
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 | Z731161 | microRNAs: Biology, Function and Expression | Providing a comprehensive overview of a fast-emerging field, this description of the biological role of microRNAs discusses methods for identifying and characterizing their functions and looks at potential applications of microRNA research in medicine and pharmacology. First identified a decade ago, microRNAs (miRNAs) are small, noncoding RNA molecules that appear to regulate gene expression. Found in plant and animal species and possibly traceable back to ancient forms of life, miRNAs are now estimated to regulate a third or more of human genes.
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