 | P2481 | 2-D Proteome Analysis Protocols | Leading investigators in the field show how to make protein extracts, reproducibly run them on 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains.
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 | A9718 | Actin | This book describes actins as highly conserved proteins found in virtually all eukaryotic cells. They have prolific roles in cell motility, from the contraction of striated muscle to the movement of organelles within cells, and are known to interact with a diverse number of proteins. This edition gives a comprehensive account of actin sequence, mutation, and structure as well as providing insight into ligand-binding sites and drug and toxin binding.
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 | A2719 | Adhesion Protein Protocols | The cutting edge methods in this title will enable both novice and experienced researchers readily to identify and clone new adhesion proteins, to successfully produce inhibitors of the adhesive activity, to develop biological models for the assay of cell-to-matrix and cell-to-cell adhesion in vitro, and to measure the signaling activities of adhesion proteins. By illuminating these adhesive molecules and the possibilities for manipulating them, the new experimental strategies collected here will have considerable clinical potential for the regulation of immunity, inflammation, tissue remodeling, and embryonic development.
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| | Z374113 | All About Albumin: Biochemistry, Genetics, and Medical Applications | Provides complete coverage of all aspects of serum albumin in one volume. It summarizes the chemistry, genetics, metabolism, clinical implications, and commercial aspects of this essential protein. Additionally, up-to-date sequences, structures, and compositions of many species can be found.
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 | A2344 | Antibody Fusion Proteins | Antibody fusion proteins are genetically-engineered proteins that result from fusion of immunoglobin with non-immunoglobin cDNA sequences. Using different types of genetically-engineered fusion molecules from antibodies as examples, this book examines antibody fusion proteins which are being used for a number of clinical applications. It will guide the biotechnologist through the principles and applications of these proteins for use in research and clinical medicine.
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 | Z703079 | Biomedical Applications of Proteomics | The first book to provide an overview over applications of proteomics in the discovery of new diagnostic, prognostic and therapeutic targets. Examples of applications are presented for the most important diseases like heart and cardiovascular disorders, cancer, infectious diseases and diseases of the nervous system. Aspects of pharmatoxicology as well as bioinformatics are also included. Authors form the U.S., Europe and Australia contribute with their specific expertise.
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 | B8674 | Biomembrane Transport | Considers recent advances in transport protein structure and function, along with established concepts. It distinguishes the similarities and differences in the mechanisms of action of transport proteins, and details the importance of biomembrane transport to regulation and interorgan nutrient flows and metabolism. Contains a classification scheme for all known transport proteins according to their functions and amino acid residue sequence similarities.
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| | Z378305 | Bioseparation of Proteins: Unfolding/Folding and Validation | A detailed critical review of literature on effectiveness, economics, and validation of bioseparation processes for drugs and other biological products. Key contents include: stability under various separation conditions case studies on methods development protein refolding and inactivation mechanisms. Coverage includes classical as well as modern bioseparation techniques.
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 | C3362 | Calcium-Binding Protein Protocols, Vol. 2: Methods and Techniques | Researchers review protein chemistry and behavior of this class of proteins, and provide experimental techniques for studying it both in vitro and in vivo. This second volume focuses on techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding proteins in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium-binding proteins in cells.
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 | Z702641 | Capillary Electrophoresis of Proteins and Peptides | Expert academic and pharmaceutical researchers describe their best capillary electrophoresis techniques for protein and peptide analysis. The authors present cutting-edge techniques for both capillary coatings and analytic detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Areas of special interest covered include combining CE and capillary isoelectric focusing with electrospray mass spectrometry detection to perform proteomic studies and applications in the field of protein-ligand binding
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 | Z731315 | Cell-free Protein Synthesis | With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.
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 | Z704393 | Chemical Genomics: Reviews and Protocols | An up-to-date introduction for the uninitiated into the various “flavors” of chemical genomics with a series of readily reproducible techniques for applications in organic chemistry, protein chemistry, and computer science, emphasizing the high-throughput approaches that are the essence of chemical genomics. The techniques range from the classic design of small molecules as affinity ligands for specific protein families, to in silico docking for computational approaches to protein–small molecule interactions, to producing highly diverse collections of proteins, carbohydrates, and small molecules for use in arrays containing large numbers of these molecules. There are also methods for the production of proteins and carbohydrates using different expression systems, for analyzing proteins using mass spectrometry, and for arraying proteins and carbohydrates on solid supports.
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 | Z703303 | Chemical Reagents for Protein Modification, 3rd ed. | Revised and updated, the third edition is an encyclopedic work describing the many approaches to the site-specific modification of proteins. More than 2,000 references are cited describing the development of the various reagents. The book explores how the use of site-specific protein modification relates to a wide variety of biotechnology processes and products, such as protein microarrays, hydrogels, controlled-release pharmaceuticals, and biotherapeutics, and proteomics. This edition presents the most frequently used methods for the site-specific chemical modification of proteins, techniques for protein characterization, precise laboratory data for factors that influence reactivity and reproducibility, and industry-specific resources.
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| | C8430 | Chemistry of Protein Conjugation and Cross-Linking | This book explains the mechanisms of chemical modification. It also surveys the bifunctional reagents used in bioconjugation and cross-linking, and provides a review of practical applications of these reagents.
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 | Z702161 | Co- and Posttranslational Proteolysis of Proteins: the Enzymes Vol. XXII | This volume examines a number of different proteases, a type of enzyme, that are required in order for the change to a biologically active mature protein to occur. The discussion of these various proteases is rarely undertaken in one volume and will serve as a great resource for scientists studying the group of proteases on signal peptide processing as well as those working on propeptide processing. These areas of research do not normally overlap, and yet they are each of common importance to the same cell processes.
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 | Z340782 | Crystallization of Nucleic Acids and Proteins: A Practical Approach, 2nd ed | This new edition provides a guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all new techniques such as uses of molecular biology in structural biology (sequence modifications to enable crystallization), diagnostic analysis of prenucleation and nucleation by spectroscopic methods, and the two-dimensional electron crystallography of soluble proteins on planar lipid films.
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 | Z731366 | Current Protocols Essential Laboratory Techniques | From the leading branded source for methods in the life sciences, this book provides every researcher with the skills and understanding of fundamental laboratory procedures to ensure greater success at the bench. It takes the novice researcher from the very basics of reagent preparation to the use of routine instrumentation found in most life science research laboratories around the world. This text teaches new investigators how to perform basic laboratory research techniques, describes solution chemistry and preparation, and covers basic laboratory safety. It also describes care and use of common equipment such as pH meters, spectrophotometers, centrifuges, and microscopes, and teaches how to manage information from lab notebooks, images, literature references, as well as manuscript preparation
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 | Z701211 | Directed Evolution Library Creation: Methods and Protocols | A compendium of cutting-edge protocols for the generation of molecular diversity. These protocols include reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologus recombination, and for constructing in vivo libraries in bacteria and yeast. This volume also describes ways to analyze libraries, particularly those made by recombination. This volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. An accomapnying volume is Directed Enzyme Evolution: Screening and Selection Methods (see Z70,131-9)
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 | Z700622 | E. coli Gene Expression Protocols | This book is a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The author's focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and protein engineering.
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 | F3299 | Foundations of Structural Biology | Understanding the implications of the three-dimensional coordinates for a molecule with several thousand atoms requires an understanding of, and practice with, 3D imaging. For many biologists, this means acquiring a whole new set of skills. This book is aimed at helping develop visualization skills for protein or DNA segments, while also describing fundamental principles underlying organization and interaction between these complex molecules.
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 | Z705217 | Green Fluorescent Protein: Properties, Applications and Protocols, 2nd ed. | This text tackles both theory and practice, offering numerous case studies, examples, illustrations, and troubleshooting tips. It examines how Ps are tailored for specific systems and used to maximize expression, and how variants are generated with altered properties. The text also explores new ways to use FPs and methods for enhancing detection in a variety of organisms and cell types. This updated and expanded edition places emphasis on the rise of FPs and their applications in industry and biosensor research. It provides new chapters on FPs, biosensors, and advances in the use of FPs in the biotechnology and pharmaceutical industries, detailed information about protocols using FPs, and three-dimensional structure, and molecular biology and mutation of FPs.
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 | Z701890 | HPLC of Peptides and Proteins: Methods and Protocols | Hands-on experts from academia and industry describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology
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 | Z701289 | Handbook of Proteomic Methods | A compendium of thirty-four powerful techniques for identifying and analyzing the diversity of proteins expressed in cells. These readily reproducible proteomic methods range from general to specific techniques, and include methods for data analysis, posttranslational modification, and its variants and isoforms. Additional methods demonstrate the application of proteomics to the discovery of serological tumor markers, to identifying the determinants of sensitivity to antitumor drugs, and to specialized fields, such as endocrinology, plant biology, nephrology, and urology.
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 | Z378283 | Heparin-Binding Proteins | By way of review, this volume starts with the structure and biosynthesis of heparin and heparan sulfates (heparinoids). The remainder concentrates on the metabolism, characterization, and biological roles of heparinoid proteoglycans. Because they are so diverse, the discussion enters into hemostasis, lipoprotein metabolism, fibroblast growth factors, and superoxide dismutase activity. A brief but key discussion focuses on using affinity chromatography for purifying and analyzing proteoglycans.
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 | Z704016 | Industrial Proteomics: Applications for Biotechnology and Pharmaceuticals | This book covers both basic elements and the state-of-the-art in applications of proteomics. The first section gives an introduction to the field, from differential approaches to functional proteomics, including yeast two-hybrid, mass spectrometry, mapping of post-translational modifications, and NMR spectroscopy. The second section offers a complete picture of industry studies in proteomics for human health and biotechnology applications. The final section addresses the future, looking at the most promising areas such as protein arrays and the integration of genomic and proteomic tools.
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 | I6029 | Interpreting Protein Mass Spectra: A Comprehensive Resource | This guide for researchers investigating structures of proteins and peptides focusses on interpretation of structural information gathered through electrospray ionization-mass spectrometry (ESI-MS). It will also provide background and protocols for anyone using matrix-assisted laser desorption/ionization (MALDI), fast atom bombardment (FAB), and secondary ion mass spectrometry (SIMS). It includes practical examples, discussions of experimental setups, and hints for troubleshooting both methods and handling of materials.
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 | Z701033 | Introduction to Macromolecular Crystallography | This book provides a comprehensive summary of the field of crystallography, fundamental theory of diffraction and properties of crystals, and applications in determining macromolecular structure. Without assuming a background in physics or math, book’s contents flow logically from basic principles to methods, such as those for solving phase problems, interpretation of Patterson maps, and the difference Fourier method. This introduction includes practical instruction on interpretation of data and methods for determining phases and also illustrates concepts throughout to clarify difficult ideas; integrates analogies to illuminate the text; equips the reader to employ the range of programs in crystallography.
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 | I3529 | Introduction to Protein Architecture | The structures and functions of proteins unlock the secrets inherent in genomes. The emphasis is on proteins as three-dimensional patterns. It explains the general characteristics of proteins that underlie the great variety of folding patterns observed in nature. Topics treated include: Pattern and form in protein structure; Secondary, supersecondary and tertiary structure; Classifications and hierarchies of protein folding patterns; Protein evolution; How proteins change conformation (and why).
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 | I0891 | Introduction to Protein Structure, 2nd ed. | This edition gives an account of the principles of protein structure, with examples of key proteins in their biological context illustrated to illuminate the structural principles described. The first chapters introduce the general principles of protein structure. Subsequent chapters use specific examples of proteins to show how they fulfill a wide range of biological functions. It concludes with chapters on the experimental approaches to determining and predicting protein structure, as well as engineering new proteins to modify their functions.
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 | P0741 | Introduction to Proteomics:Tools for the New Biology | This is an introduction to proteomics, a hot area in the biological sciences which enables scientists to study the protein complement of the genome. It refers to studying the functions of many proteins at one time, as opposed to how research was conducted in the mid-90's where the technology did not enable the study of the entire protein system. From this, one is able to develop an overview of the way a genome functions and the role it plays in health and disease.
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 | Z702242 | Isolation and Purification of Proteins | This book discusses rational design of downstream protein processing; product release from cells and tissues by mechanical disruption, chemical agency and enzymatic lysis; genetic strategies to facilitate purification of recombinant proteins; solid-liquid spearation via centrifugation, flocculation, filtration and filter aids; the usage of membrane filtration for protein isolation; nonselective and selective precipitation; protein crystallization on a large scale; extraction in aqueous two-phase systems and expanded bed absorption for rapid protein isolation; robust, superporous chromatography matrices for in crude feeds, and more.
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 | M7059 | Mass Spectrometry of Proteins and Peptides | This new collection of cutting-edge methods in analyzing proteins and peptides is a revision of the acclaimed 1996 book. Each chapter provides background information before guiding a researcher step-by-step through the experimental process, with detailed instructions to help assure experimental success. Applications covered include protein sequencing, higher-order structure determination, epitope mapping, kinetics, quantitation, glycosylation analysis, and bacterial typing.
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 | M9937 | Mechanisms of Protein Folding, 2nd ed. | Process by which polypeptide chains become their final 3-dimensional protein forms is clearly explained. Second edition incorporates significant improvements such as advances in interpreting observed kinetic data, development of technology to observe fast folding reactions, molten globule state, and vital role of chaperone proteins in protein folding. New discoveries of the central importance of protein folding and unfolding reactions in biology and medicine, including mutation and misfolding, are explored. Three case studies elucidate the difficulties of studying protein folding in vivo.
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 | Z701203 | Membrane Protein Protocols: Expression, Purification, and Characterization | A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocols contains step-by-step instructions, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.
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 | Z703737 | Metabolomics by In Vivo NMR | This book is unique in linking in vivo 13C NMR measurements of neuronal activity and energetics with applications to functional imaging and certain disease states. It provides a fundamental neurochemical explanation of brain activity applicable to functional imaging, theories of neuronal activity and disease states, e.g. epilepsy, psychiatric diseases and developmental disorders It will inspire future research directions
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 | Z702463 | Methods and Results in Crystallization of Membrane Proteins | Parts I and II of this book deal with general principles and techniques in membrane protein crystallization in a clear and concise fashion, with an excellent general introdution followed by chapters covering the use of detergents, crystallization in lipidic cubic phases, the use and generation of antibody fragements, porin as a model and crystalliztion of membrane proteins in oil. Part III focuses on specific examples of membrane proteins whose structures have been solved. The examples cover the gamut of membrane proteins from those involved in photosynthesis to respiratory complexes to channels, receptors and the outer membrane protein phospholipase A.
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| | MIE201 | Methods in Enzymology Series Protein Phosphorylation Part B, Vol 201 | A selection of titles from the long-running series published by Academic Press.
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 | M9807 | Microcharacterization of Proteins, 2nd Edition | This thorough introduction and laboratory guide details the most recent developments in protein separation and characterization, fragmentation and micro sequencing. It offers a stimulating combination of basic concepts and practical applications and presents the latest information in the rapidly emerging area of protein characterization.
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 | M9814 | Modern Protein Chemistry: Practical Aspects | This is a lab cookbook covering all the advanced techniques for working with proteins. Simple directions for each technique are presented along with references, estimated of time, special equipment required, and points on troubleshooting. This gives researchers a "cost-benefit" analysis of these techniques. It also provides hands-on information to complement theoretical understanding. No other guide exists that enables a researcher to make a practical evaluation of these methods.
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 | Z703508 | Modular Protein Domains | Since the full functionality of any given protein can only be understood in terms of its interaction with other, often regulatory proteins, this unique reference source covers all relevant protein domains, including SH2, SH3, PDZ, WW, PTB, EH, PH and PX. Its user-oriented concept combines broad coverage with easy retrieval of essential information, and includes a special section on Web-based tools and databases covering protein modules and functional peptide motifs. Essential for the study of protein-protein interactions in vivo or in silico, and a prerequisite for successful functional proteomics studies.
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 | Z705381 | New and Emerging Proteomic Techniques | Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular Biology™ series format.
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 | N6410 | Novel Therapeutic Proteins: Selected Case Studies | This book describes medical applications of recombinant proteins and monoclonal antibodies, some of which have already been on the market for several years while others have only recently been launched. It also highlights the manufacturing processes for individual products, the strategies that were taken by companies in the clinical development, and the hurdles that were encountered in clinical trials. Finally, this book illustrates strategies to modify and improve the pharmacodynamic and pharmacokinetic properties of naturally occurring proteins.
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 | Z700215 | Peptide Arrays on Membrane Supports: Synthesis and Applications | Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding. The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented.
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 | Z340839 | Post-translational Processing: A Practical Approach | This book and its companion volume Protein Expression: A Practical Approach form the final part of the PAS mini-series on protein synthesis and processing. This volume covers protein sequencing, protein folding and import, organelles, and the three major forms of covalent modification--phosphorylation, glycosylation, and lipid modification. It is an ideal resource for research on protein expression in cell free systems, viruses, and prokaryotic and eukaryotic cells
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 | Z704636 | Posttranslational Modification of Proteins: Expanding Nature’s Inventory | This book covers the wide range of posttranslational modifications found in Nature, many of which have been discovered relatively recently as a result of improved detection methods. It may be used as a reference or as a collection of the structural and functional variety of these modifications. The author covers the subject from the perspective of both the mechanism of formation of these protein conjugates and their biological function in processes like signal transduction and enzymatic catalysis. Several links are made between various modification processes and their roles in biological processes that tie together the heterogeneous collection of posttranslational modifications.
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 | P8742 | Posttranslational Modification of Proteins: Tools for Functional Proteomics | This volume describes reproducible methods for detecting and analyzing the posttranslational modifications of protein, particularly with regard to protein function, proteome research, and the characterization of pharmaceutical proteins. Methods include those for analyzing the assignment of disulfide bond sites in proteins, protein N-glycosylation and protein O-glycosylation, and oligosaccharides present at specific single glycosylation sites in a protein. Additional techniques facilitate the analysis of glycosylphosphatidylinositols, lipid modifications, protein phosphorylation and sulfation, protein methylation and acetylation, a-amidation and lysine hydroxylation
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 | Z364010 | Practical Guide to Membrane Protein Purification | Written for those who have some familiarity with separation of water-soluble proteins, but who may not be aware of the problems involved in the isolation of membrane proteins. This book is an essential manual for investigations of structure and function of native membrane proteins.
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| | Z350346 | Practical Guide to Protein and Peptide Purification for Microsequencing | This second edition provides a guideline for obtaining useful sequence information from proteins or peptides. Important new methods include ways of dealing with blocked N-termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
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 | P4984 | Practical Protein Crystallography, 2nd ed. | Designed for easy use by both beginning and experienced protein crystallographers, the second edition of Practical Protein Crystallography is an essential handbook for any scientist interested in solving a protein structure. The book includes examples of actual experiments and data, electron density maps, and computer methods. This second edition has new material covering CCP4, SHELX, cryocrystallography, MAD and automated fitting.
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 | Z703109 | Principles and Reactions of Protein Extraction, Purification and Characterization | This book provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different methods. The book also discusses the development of antibodies and immunochemical techniques as tools for characterizing proteins and modified proteins such as glycoproteins. Along with methodical working procedures for most techniques, the book also offers useful advice on which technique to use and when to apply a particular method.
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 | Z703494 | Principles of Proteomics | This book provides a comprehensive introduction to this relatively new field, for the first time bringing together all the disparate themes of proteomics. The book covers the development of large-scale technologies for protein separation, isolation, detection and quantitation. It describes in detail the role of mass spectrometry in high-throughput protein annotation. It also covers the emerging fields of structural proteomics, interaction proteomics and the recent development of chip-based analysis techniques. The final chapter provides some examples of how proteomics is being applied in the fields of medicine, biotechnology and agriculture. Each chapter ends with a short bibliography containing some classic papers and useful reviews for further reference.
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 | Z703427 | Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems | The choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, but most researchers do not have ready access to pertinent information that is scattered throughout the scientific literature. This book provides information on the general biology of the host organism, a description of the expression platform, a methodological section as well as examples of proteins produced with the respective platform. The systems described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems. The book provides valuable information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation.
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 | Z373907 | Protein Analysis and Purification: Benchtop Techniques | This manual guides the reader through 185 proven and productive methods for both soluble and membrane proteins. Topics covered include protein labeling, protein modification analysis, recombinant protein expression, chromatography, electrophoresis and more. A must for all workers in the laboratory.
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 | Z702323 | Protein Arrays, Biochips, and Proteomics: The Next Phase of Genomic Discovery | From disease marker identification to accelerated drug development, this reference offers a detailed overview of current and emerging trends in the field of proteomics—focusing on innovations in protein microarrays and biochips, mass spectrometry, high-throughput protein expression, protein–protein interactions, structural proteomics, and the proteomic marketplace for comprehensive understanding of proteomic research in the past, present, and future
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 | Z702749 | Protein Arrays: Methods and Protocols | A diverse collection of unique methodologies to synthesize and construct protein arrays for basic and clinical research in a high-throughput manner. These readily reproducible techniques can be applied to such applications as quantifying specific proteins of interest and discovering novel proteins, as well as to a variety of affinity substances, including antibodies, peptides, proteins, aptamers, and chemicals. The techniques can be used for biomarkers discovery, assay development, clinical sample testing, and building the next generation of molecular tools
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 | Z702013 | Protein Crystallography in Drug Discovery | Protein structures suited for rational drug design are almost exclusively derived from crystallographic studies, and drug developers are relying heavily on the power of this method. In this book, researchers from academia and from leading pharmaceutical companies present valuable first-hand information on how to obtain the desired protein structures and how to exploit crystallographic data for the drug discovery process. Topics include: Crystallography of important target classes such as kinases and proteases; Structure-based design of protease inhibitors; Structure-based design of kinase inhibitors; Ribosome and the proteasome as drug targets; Recent methodological advances in high-throughput crystallography and microcrystallization.
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 | Z705160 | Protein Degradation: Ubiquitin and the Chemistry of Life, volume 1 | The first volume in a new series dedicated to protein degradation, this book explains the fundamentals of targeted protein breakdown via the ubiquitin pathway. Drawing on the combined knowledge of the world's leading protein degradation experts, this handy reference compiles information on the initial steps of the ubiquitin pathway. Starting out with a broad overview of protein degradation and its functions in cellular regulation, it then goes on to discuss the molecular mechanisms of ubiquitin conjugation and recycling in detail. All currently known classes of ubiquitin protein ligases are treated; this includes latest structural data on these enzymes.
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| | Z371955 | Protein Engineering: Principles and Practice | Designing proteins with novel structures and properties requires a clear understanding of natural structure-function relationships. This book introduces the main concepts of protein folding, conformation prediction, metal binding sites, and generation of therapeutic antibodies. Also covered: aspects of protein structure and function as relates to drug design.
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 | Z340820 | Protein Expression: A Practical Approach | Details of the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems are included such as cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes and prokaryotic cells. Cell-free systems of both eukaryotes and prokaryotes are described in addition to the prokaryotic systems that offer coupled transcription-translation.
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| | P1568 | Protein Folding | This book presents key experiments and investigative techniques as well as the implications of recent findings for modern biotechnology and biomedicine. The protein folding problem refers to the difficulty in determining how and why a protein adopts a particular conformation, given that the number of possible conformations for an average-sized protein approaches 10 to the 270th power. This subject is summarized in chapters on the physical basis of stability of folded conformations, mutational analysis of folding and stability, folding pathways, and other avenues of exploration.
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 | P2244 | Protein Folding in the Cell: Advances in Protein Chemistry Volume 59 | This volume of Advances in Protein Chemistry provides a broad, yet deep look at the cellular components that assist protein folding in the cell. This area of research is relatively new. Ten years ago these components were barely recognized, so this book is a particularly timely compilation of current information. Topics covered include a review of the structure and mechanism of the major chaperone components, prion formation in yeast, and the use of microarrays in studying stress response.
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| | P7548 | Protein Function: A Practical approach | The analysis of protein function is a vital step in the characterization of any newly discovered protein. This new edition brings up to date the techniques used, and presents experimental procedures that can be performed in the average laboratory without recourse to highly specialised equipment. The protocols will be of use to both experienced and novice researchers and are accompanied by background information, hints and tips, and troubleshooting guides to ensure successful elucidation of protein function.
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 | P3113 | Protein Kinase Functions | This provides a new focus on context and function of protein kinases, thus reflecting recent advances in kinase biology. Chapters on genetic approaches to protein kinase functions, MAP kinase pathway, and cyclin-dependent kinases are updated. Topics covered in depth are phosphatidylinositol 3-kinase signaling, JAK-STAT signaling, suppression of tyrosine kinases by the SOCS family proteins, TGFss superfamily, and involvement of protein kinases in response to DNA damage. Throughout, emphasis is placed not on individual kinases, but on functional aspects of the whole system and relationship between processes and molecules.
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 | P5611 | Protein Kinase Protocols | Basic and more sophisticated methods are described for the analysis of kinase-mediated signaling cascades, with emphasis on the identification of proteins according to their interactive relationships and the analysis of their functional properties. Emphasis is given to critical technical steps that are often omitted from other published methods. This offers tools for understanding the functional roles of specific protein kinases within signaling cascades and for identification and evaluation of novel therapeutic targets.
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 | P9359 | Protein Lipidation Protocols | This is a collection of reproducible techniques for studying protein lipidation. These methods, deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Included are techniques for determining the chemical structure of GPI-anchors, for radiolabeling the prenyl groups of protein in eukaryotic cells, a tool for developing inhibitors of the protein farnesyltransferase, and for a lysosomal enzyme that cleaves fatty acyl groups from proteins.
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 | Z340847 | Protein Localization by Fluorescence Microscopy: A Practical Approach | The ability to determine the location of gene products within a cell by the use of antibodies or by production of chimeras with green fluorescent protein is a vital step towards understanding what they do. This guide provides detailed, practical advice from choosing the right equipment to interpreting results. It balances the advantages of a range of techniques, including live cell work, against potential pitfalls, offering invaluable "tricks of the trade" along the way.
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 | P6308 | Protein Methods, 2nd ed. | Revised and expanded, this edition remains the key source for tested and proven techniques. Four new chapters are devoted to protein purification by ion exchange, gel filtration, and affinity chromatography, and to protein crystallization.
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 | Z701734 | Protein Microarray Technology | This book is the first of its kind in the field of protein microarrays and addresses novel strategies for constructing highly functional and biocompatible microarrays for screening proteins. The authors provide a roadmap for solving the complex challenges that are currently faced while monitoring protein-protein interactions over a wide range of microarray platforms. In doing so, they also offer a comprehensive overview of microarray surface chemistry, detection technologies, fabrication options for array development, and data analysis of numerous types of protein interactions. Topics covered include: Types of biomolecular interactions; Surface chemistry; Detection technologies; Spotting technologies; Bioinformatics/data analysis.
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 | Z701882 | Protein Misfolding and Disease: Principles and Protocols | A comprehensive review of the latest thinking about the molecular processes underlying conformational diseases, combined with a remarkable set of biochemical, genomic cellular, and chemical laboratory techniques for studying their genesis and pathologies. The authors apply their carefully refined methods to a variety of metabolic and neurodegenerative disorders, as well as to the aging process. The techniques presented are broadly applicable in many diverse disease contexts and may be used in both diagnosis and research on new treatment strategies.
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 | Z702846 | Protein NMR Techniques, 2nd ed. | This collection of reproducible methods using the latest advances in NMR technology includes methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation protocols, and solid-state methods. It offers detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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 | Z703680 | Protein Nanotechnology: Protocols, Instrumentation and Applications | Experts in nanobiotechnology review the recent advances in instrumentation and methodology, and their applications in genomics and proteomics. Authors provide a wide variety of techniques and methods for dealing with protein functions and structures at the nanoscale level, including nanostructured systems, nanomaterials, carbon nanotubes and nanowires, optical nanosensors, and nanoelectrodes. Among the highlights are techniques for the in vivo tracking of biochemical processes using fluorescent molecular probes and nanosensors, and the development of nanocarrier methodology for the targeted delivery of drugs whose shells are conjugated with antibodies for targeting specific antigens.
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 | P1478 | Protein Phosphatase Protocols: Methods in Molecular Biology, Vol. 93 | Cutting-edge techniques presented with step-by-step instructions make this indispensable for investigating the structure and function of protein serine/threonine phosphatases. Contains methods designed to isolate, identify, and assay phosphatase activities from both prokaryotic and eukaryotic sources. Some of the points covered are step-by-step instructions on how to isolate novel phosphatases and regulatory subunits; detailed methods for generating immunological reagents to characterize protein phosphatases; cloning of novel phosphatases and regulatory subunits; notes about potential pitfalls and tips on how to avoid failure.
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 | Z340855 | Protein Phosphorylation: A Practical Approach, 2nd Edition | This new edition provides a description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells, analysis of signal transduction pathways, kinase specificity and kinase interactions, assay and purification of kinases and phosphatases, and identification of substrates.
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 | P4742 | Protein Protocols Handbook, 2nd ed. | This edition provides a cross-section of analytical techniques commonly used for proteins and peptides. The text is significantly updated and expands on a number of chapters from the first edition. The Notes section complements the Methods by indicating how best to deal with any problem or difficulty that may arise when using a given technique. Further chapters examine the developments in the use of mass spectrometry in protein characterization. Recognition of the central role of 2-D PAGE in proteomics has resulted in an expansion of chapters on this subject, as well as a number of new techniques for staining and analyzing protein blots.
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 | P7618 | Protein Protocols on CD-ROM, Version 2.0 | Here is instant access to over 5000 tested, state-of-the-art protein methods and supporting protocols that are reproducible and accessible on a CD-ROM. Unprecedented in scope and depth of coverage, this collection contains the major procedures needed for isolating, characterizing, and analyzing proteins, including powerful techniques in mass spectroscopy, NMR, proteomics, and immunochemistry. This CD-ROM includes cutting-edge NMR techniques, physical methods of analysis, and protocols for peptide synthesis, membrane proteins, ELISA, proteolytic enzymes, and epitope mapping.
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 | P5238 | Protein Purification Applications: A Practical Approach, 2nd ed. | With protein purification workload reduced by improved techniques, there is still a need to consider how unit operations link together to form a purification strategy. This book describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris is a work of art.
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 | Z701483 | Protein Purification Protocols, 2nd ed. | Experts update this classic protocols manual for protein purification techniques. New chapters include those on protein fractionation and chromatographic techniques. New developments in proteomics are included such as the use of 2-D gel electrophoresis as a preparative technique for protein characterization. They also include newer techniques using analytical chromatography for the multidimensional separations of proteins and peptides as well as mass spectrometric techniques for isolating proteins. The authors provide descriptions of how to scale-up purification methods and the purification of proteins for therapeutic use.
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 | P5363 | Protein Purification Techniques: A Practical Approach, 2nd ed. | Protein purification techniques have evolved with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. This book focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations.
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 | Z379883 | Protein Purification: Principles, High Resolution Methods, and Applications, 2nd ed. | State-of-the-art coverage of chromatographic and electrophoretic protein separation and characterization methods. Balances theory and applications, offers professionals and students access to all the important techniques for analytical and small- and large-scale purification of proteins.
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 | Z700282 | Protein Sequencing Protocols, 2nd ed. | This book is an update to the much-acclaimed first edition with new and updated techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to analysis of protein sequence information via the powerful new tools of bioinformatics. This comprehensive book provides investigators a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination.
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 | P3363 | Protein Sequencing and Identification Using Tandem Mass Spectrometry | Readers will find in this book complete experimental detail to apply mass spectrometry techniques in their laboratoriess and learn about the proper selection and operation of instruments. Coverage includes peptide fragmentation and interpretation of product ion spectra, preparation of protein digests for sequencing experiments, mass spectrometric analysis using capillary liquid chromatography, characterization of modified peptides using tandem mass spectrometry and techniques for protein identification by database searches.
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 | P2494 | Protein Staining and Identification Techniques | Staining techniques are fundamental tools for the study of proteins, but information on protein-specific staining procedures has remained scattered. This book presents a spectrum of protein staining procedures, including wool-dye, metallic, enzyme, fluorescence, and immunostaining, the latter both in situ and following transfer to membranes. Many of these stains have their origins as histochemical techniques. Finally, techniques for obtaining photographic or electronic images of gel separations are presented in a section on the preparation, archiving, and presentation of gel reproductions.
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| | Z375470 | Protein Structure Prediction: A Practical Approach | Describes in detail the various computer-based strategies for translating the sequence of proteins into structure, highlighting the degree of confidence that can be attributed to different algorithms. Covers the principles of protein folding, “secondary structure,” and tertiary fold recognition. Invaluable reference for those requiring up-to-date information on the latest techniques in the field.
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 | Z701254 | Protein Structure Prediction: Bioinformatic Approach | An example of new generations of software is in the field of protein structure prediction. Here new approaches to fold recognition, structural alignment and threading continue to appear at a rate that leaves the investigator at a loss of which way to turn to solve any particular problem. This book serves as an excellent guide to the very newest approaches. A wide variety of programs and strategies are discussed, including new applications of hidden Markov models and novel Bayesian approaches for building up model from block fragments. The theme throughout is determining three-dimensional structures for the plethora of newly determined amino acid sequences.
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 | Z705829 | Protein Structure Prediction: Concepts and Applications | Protein structure prediction is evolving as a component of the standard tools of the molecular biologist. While most books on bioinformatics focus on genetic algorithms and treat protein structure prediction only superficially, this book assumes a novel and unique focus. Adopting a didactic approach, the author explains all the current methods in terms of their reliability, limitations and user-friendliness. Knowing how far to trust predictions, one must understand how the methods work. Topics covered include homology modeling, secondary structure prediction, fold recognition and prediction of three dimensional structure of proteins with novel folds. The author also provides practical examples to help first-time users become familiar with the possibilities and pitfalls of computer-based structure prediction.
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 | P6988 | Protein Structure, Stability and Folding | Volume 164 of the Methods in Molecular Biology series describes the newest experimental and theoretical methods. Among the techniques discussed are the methods for calculating aspects of protein stability and dynamics from knowledge of the structure, and for performing molecular dynamics simulations of protein unfolding. New experimental approaches presented include the use of co-solvents, novel applications of hydrogen exchange techniques, temperature-jump methods for looking at folding events, and new strategies for mutagenesis experiments.
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 | Z705195 | Protein Synthesis and Ribosome Structure | Based on more than 30 years of ribosome research and recent structural evidence, this book presents a uniform picture of the largest enzyme complex found in living cells, shedding light on many decades-old questions in molecular biology. Topics include Structure of the Ribosome; Ribosome Assembly; tRNA and Synthetases; mRNA Degradation; Initiation and Elongation; Termination and Ribosome Recycling; Regulation of Ribosome Biosynthesis; Inhibition of the Translational; and Apparatus by Antibiotics.
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| | P2356 | Protein Synthesis: Methods and Protocols | This book presents a collection of molecular biological methods specific to protein synthesis. Chapters open with a discussion of basic background information and strategy which is then complemented by comprehensive methodological details. The book is divided into seven significant areas that cover all of the research techniques required by both experienced researchers and newcomers to the field of protein synthesis, and will prove to be an invaluable reference source on the benchtop of many protein laboratories.
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 | Z374008 | Protein Targeting | | pricing |
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 | P8739 | Protein-Ligand Interactions: A Practical Approach 2 volume set | The two Practical Approach volumes on protein-ligand interaction do not comprise a comprehensive compilation of all the methods that can be used to investigate protein-ligand interactions. Instead, they are a selection of the most useful and easily applied methods and will be an invaluable guide to the principal techniques used to study the interactions of proteins and ligands.
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| P8864 | Protein-Ligand Interactions: A Practical Approach Hydrodynamics and Calorimetry, Volume 1 | This volume covers the principle hydrodynamic and calorimetric techniques for studying protein-ligand interactions. It includes equilibrium dialysis, rate dialysis, quantitative characterization by chromatography, sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation for probing ligand binding, surface plasmon resonance, capillary electrophoresis, molecular electro-optics, high flux x-ray and neutron scattering, isothermal titration calorimetry and differential scanning microcalorimetry.
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| P8989 | Protein-Ligand Interactions: A Practical Approach Structure and Spectrocopy, Volume 2 | This second volume is a compilation of key spectroscopic and other high resolution techniques for studying protein-ligand interactions. It includes x-ray crystallography, molecular modelling, fluorescence real time kinetics, spatial imaging, stopped flow techniques, Fourier Transform infrared spectroscopy, Raman and resonance-Raman spectroscopy, electrospray ionization mass spectrometry, spin label paramagnetic probes, nuclear magnetic resonance and atomic force microscopy.
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 | Z701181 | Protein-Ligand Interactions: From Molecular Recognition to Drug Design | The lock-and-key principle has still not lost any of its significance. The basic aspects of ligand-protein interaction may be summarized under the term ‘molecular recognition’ and concern the specificity as well as stability of ligand binding. Molecular recognition is thus a central topic in the development of active substances, since stability and specificity determine whether a substance can be used as a drug. Nowadays, computer-aided prediction and intelligent molecular design make a contribution to the constant search for improved enzyme inhibitors, and new concepts such as that of pharmacophores.
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 | Z704180 | Protein-Ligand Interactions: Methods and Applications | A collection of techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. Protocols follow the series format, offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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 | Z704792 | Protein-Lipid Interactions: From Membrane Domains to Cellular Networks | This first handbook in the field covers the interactions between proteins and lipids that make the fabric of biological membranes from every angle. It examines the relevant thermodynamic and structural issues from a basic science perspective, and goes on to discuss biochemical and cell biological processes. The book covers physical principles as well as mechanisms of membrane fusion and fission. Additionally, chapters on bilayer structure and protein-lipid interactions as well as on how proteins shape lipids and vice versa, membrane penetration by toxins, protein sorting, and allosteric regulation of signal transduction across membranes make this a valuable information source.
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 | P0241 | Protein-Protein Interactions: A Molecular Cloning Manual | As more genetic and biochemical information about the protein components of cells accumulates, the analysis of protein-protein interactions is becoming increasingly important. This manual presents a wide range of techniques for identifying and analyzing these interactions, starting with standard molecular and biochemical techniques, and progressing to biophysical and computational approaches and therapeutic and other post-genomic applications. This manual is a complement to the best selling laboratory manual "Molecular Cloning".
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 | Z718629 | Protein-Protein Interactions: A Molecular Cloning Manual | As more genetic and biochemical information about the protein components of cells accumulate, the analysis of protein-protein interactions is becoming increasingly important. This manual presents a wide range of techniques for identifying and analyzing these interactions, starting with standard molecular and biochemical techniques, and progressing to biophysical and computational approaches and therapeutic and other post-genomic applications. This manual is designed to complement the information in the best-selling Molecular Cloning manual, and is presented in the same clear, user-friendly format. It is an essential resource for investigators studying interacting sets of proteins and their physiologic significance in a wide range of experimental systems.
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 | Z719145 | Protein-Protein Interactions: A Molecular Cloning Manual, 2nd Edition hard cover | This is an updated edition of a manual that provides a thorough collection of the technical and theoretical issues involved in the study of protein associations, including standard methods, biophysical approaches, and, in a final section, a collection of computational methods for integrating and analyzing interactions.
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| Z719153 | Protein-Protein Interactions: A Molecular Cloning Manual, 2nd Edition soft cover | This is an updated edition of a manual that provides a thorough collection of the technical and theoretical issues involved in the study of protein associations, including standard methods, biophysical approaches, and, in a final section, a collection of computational methods for integrating and analyzing interactions.
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 | Z702471 | Protein-Protein Interactions: Methods and Protocols | This collection of successful techniques demonstrate how to identify protein interaction partners, qualitatively or quantitatively measure protein-protein interactions in vitro or in vivo, monitor protein-protein interactions as they occur in living cells, and determine interaction interfaces. The techniques described utilize a variety of cutting-edge technologies, including surface plasmon resonance (SRP), fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), isothermal titration calorimetry (ITC), circular dichroism (CD), protein fragment complementation assays (PCA), various two-hybrid systems, and proteomics and bioinformatics-based approaches.
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 | P1619 | Protein-Protein Recognition | This book brings together concepts and systems pertaining to protein-protein interactions in an examination of how and why proteins form complexes with each other. The emphasis is on heteromeric complexes and uses model systems to explain the processes of forming complexes. There is a section on the kinetics, thermodynamics, analysis, and classification of protein-protein interactions. MHC proteins and their interactions with T-cell receptors is used to explain complexes with intermediate affinities. The themes used are the technologies which have had the greatest impact, how specificity is determined, how complexes are stabilized, and medical and industrial applications.
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| | Z373648 | Proteins Labfax | | pricing |
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 | Z700428 | Proteins and Proteomics: A Laboratory Manual | Completion of sequencing of the human genome has opened the door for exploration of the proteome. Critical to tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying, isolating, and quantifying proteins in cells and cell pathways and for performing functional assays. This book provides the first authoritative and protocol-based approach to proteomics. It provides information about protein structure and numerous methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics. Extensive background information and references are provided regarding the theoretic aspects of the techniques and their applications.
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 | Z718416 | Proteins and Proteomics: A Laboratory Manual | Completion of sequencing of the human genome, as well as those of many other organisms, has now opened the door for exploration of the proteome, the many thousands of interacting proteins in a given organism. Critical to tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying, isolating, and quantifying proteins in cells and cell pathways and for performing functional assays. Proteins and Proteomics: A Laboratory Manual, authored by Richard Simpson of the Ludwig Institute for Cancer Research, Melbourne, provides the first authoritative and wide–ranging protocol-based approach to proteomics. Presented as a logical strategy for analyzing proteomes, Proteins and Proteomics provides information about protein structure and numerous methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics. Extensive background information and references are provided regarding the theoretic aspects of the techniques presented as well as their applications. This manual is an essential laboratory tool for genetics and molecular biology investigators moving from studies of genomics and genotype to those of proteomics and phenotype. It is invaluable for cell biologists, developmental biologists, and neuroscientists exploring the intricacies of the proteome. It will also be an excellent resource for protein chemists and biochemists.
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 | P0868 | Proteins, Peptides and Amino Acids SourceBook | Building on the success of their Source Book of Enzymes, the authors have assembled a catalog of over 26,000 commercially available proteins, peptides, and amino acids. All are arranged alphabetically and by sequence for fast access, and are replete with technical details and vendor information. Compounds can be easily located by either directly searching the appropriate section by chemical name or by consulting the general index by name, synonym, or derivative formula. The data covers sequence, sequence modification, chemical derivatives, preparation form, purity, composition, activity, functionality, as well as applications and literature references.
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 | Z378321 | Proteome Research: New Frontiers in Functional Genomics | “Proteome” research is the other side of the genome project. Using high-resolution two-dimensional gel electrophoresis to analyze proteins, one can determine which genes are expressed, how the products are modified after translation, and how proteins interact. This book summarizes the state of the art in 2-D electrophoresis, proteome databases, 3-D protein modelling, and biomedical applications of proteomics. A brief, well organized introduction to this fundamentally important area of research.
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 | P4617 | Proteome Revisited: Theory and Practice of all Relevant Electrophoretic Steps | This covers electrophoretic steps leading to proteome analysis, i.e. isoelectric focusing (including immobilized pH gradients), SDS-PAGE electrophoresis and finally two-dimensional maps. It is a collection of modern methodologies leading to successful fractionation, analysis and characterization of every polypeptide spot in 2-D map analysis. It includes chapters on sophisticated mass spectrometry developments and navigating important databases. It contains several as yet unpublished protocols, correcting some of the existing ones and showing the pitfalls and limitations of others.
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 | Z731188 | Proteomic Biology Using LC-MS: Large Scale Analysis of Cellular Dynamics and Function | This is one of the first books to focus on the dynamic aspect of proteomes. The book introduces proteomics to the newcomer, reviews the theoretical aspects of proteomics and its state-of-the art technologies, along with a number of biological applications using "classical" proteomic technology. The book also presents a new concept, the Dynamome, or the expression of a comprehensive molecular set that participates in the whole dynamic process of a series of cellular events.
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 | Z701041 | Proteomic and Genomic Analysis of Cardiovascular Disease | This is the very first book to focus on this new approach that will eventually aid in the development of new diagnostic markers and therapies for controlling and treating heart disease. Divided into two parts, the book describes not only the potentials, but also the limitations of these technologies. The editors provide new insights into the biochemical and cellular mechanisms of cardiovascular disease, as well as covering the transition into clinical applications. They highlight the various strategies and technical aspects so as to assist the number of researchers intending to utilize these approaches. The result is an excellent way of educating and informing researchers about the latest developments.
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 | Z703893 | Proteomics Protocols Handbook | Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification.
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 | Z704024 | Proteomics Today: Protein Assessment and Biomarkers Using Mass spectrometry, 2-D Electrophoresis and Microarray Technology | The last years have seen a drive toward the application of proteomics to resolving biomedical and biochemical tasks. Separation techniques combined with mass spectrometry are playing a central role. This book discusses the role of mass spectrometry in proteomic research, and emphasizes advances in the existing technology and describes the advantages and pitfalls. It provides a scientifically valid method for analyzing the approximatey 500,000 proteins that are encoded in the human genome, and explains the hows and whys of using mass spectrometry in proteomic analysis. It also includes sections on troubleshooting.
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 | P5618 | Proteomics in Practice: A Laboratory Manual of Proteome Analysis | A reference for the analysis of proteins using the classical approach of 2-D gel electrophoresis, mass spectrometry and related sequence database inquiries. The first section introduces the entire technology, while the second section represents a laboratory manual spanning the range of methods from sample preparation to protein identification. The third section is a troubleshooting guide to answer questions regarding proteome analysis. The final section contains a reference list to guide readers towards further detail. Updates of recipes and animations are available at http://www.wiley-vch.de/books/info/3-527-30354-5
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 | P5986 | Proteomics: From Protein Sequence to Function | Following the announcement of the draft sequence of the human genome, attention is now increasingly turning to the analysis of the proteins encoded by genomes - proteomics. This is a rapidly emerging research area and this book reviews recent developments in proteomics and compares them with current genomics technologies. The book is written by experts who review their area in a concise yet easy to understand style, providing a comprehensive review volume that integrates genomics with proteomics.
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 | Z719269 | Proteomics hard cover | Sigma-Aldrich is taking pre-publication orders for this book now. It will be shipped when published, approximately April 2007. You will not be billed until the book is shipped.
Conventional approaches to proteomics have been augmented with a new generation of non-gel technologies unfamiliar to many life science researchers. This book identifies the most powerful of these new technologies and presents them in a way that allows their robust implementation in even non-specialist labs. The focus is on contemporary proteomic methods and strategies that are reliable and of general utility. Each chapter present realistic descriptions of what can and cannot be achieved with the relevant procedures so that readers can make informed judgments prior to establishing the methods in-house. Topics include Sample preparation and protein pre-fractionation; Isotope-coding strategies; Gel-based separations; Mass spectrometry strategies for protein identification; Extraction of diagnostic signatures; Protein chips; Bioinformatics approaches in proteomics.
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| Z719250 | Proteomics soft cover | Sigma-Aldrich is taking pre-publication orders for this book now. It will be shipped when published, approximately April 2007. You will not be billed until the book is shipped.
Conventional approaches to proteomics have been augmented with a new generation of non-gel technologies unfamiliar to many life science researchers. This book identifies the most powerful of these new technologies and presents them in a way that allows their robust implementation in even non-specialist labs. The focus is on contemporary proteomic methods and strategies that are reliable and of general utility. Each chapter present realistic descriptions of what can and cannot be achieved with the relevant procedures so that readers can make informed judgments prior to establishing the methods in-house. Topics include Sample preparation and protein pre-fractionation; Isotope-coding strategies; Gel-based separations; Mass spectrometry strategies for protein identification; Extraction of diagnostic signatures; Protein chips; Bioinformatics approaches in proteomics.
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 | P1364 | Purification and Analysis of Recombinant Proteins | Covering vital and traditional topics in the purification and analysis of recombinant proteins, this volume demonstrates how to overcome problems in protein research and presents practical methods used in protein work, explaining their theoretical basis. The collection also explores innovative concepts, such as receptor affinity chromatography and the introduction of sites that facilitate purification. This aid uses specific examples of proteins obtained from bacterial and nonbacterial sources and supplies helpful approaches and techniques.
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 | Z701793 | Purifying Proteins for Proteomics: A Laboratory Manual | This manual complements Simpson's Proteins and Proteomics manual (Sigma # Z70,042-8), with a comprehensive collection of methods for protein purification from a variety of source preparations. The protocols are accompanied by extensive discussion of potential pitfalls and troubleshooting advice.
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 | Z718947 | Purifying Proteins for Proteomics: A Laboratory Manual | This manual complements Simpson’s Proteins and Proteomics manual, with a comprehensive collection of methods for protein purification from a variety of source preparations. The chapters include detailed protocols, methods for optimizing the performance of experiments, discussion of potential pitfalls, and troubleshooting advice.
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 | Z730696 | Quantitative Proteomics by Mass Spectrometry | This book is a compendium of cutting-edge protocols for quantitative proteomics, and presents the most significant methods used in the field today. The focus on mass spectrometry (MS) is integral, as MS has, and will continue to be, an essential tool in proteomics for studying complex biological systems and human diseases. This volume, written and compiled by leading quantitative proteomic experts, is an indispensable resource in the search for novel biomarkers. Quantitative Proteomics by Mass Spectrometry presents several innovative MS quantitative procedures, including a variety of methods for introducing isotopic labels and quantifying post-translational modifications. Some of these methods include growing an organism in isotope-enriched media, performing trypsin proteolysis in the presence of 18O-water, reacting protein samples with isotopically labeled reagents, quantifying relative amount of proteins without the use of any isotopic labels. Attention is also given to state-of-the-art techniques for the characterization of the phosphoproteome and tandem MS for detection of inborn errors of metabolism. Specifically, the procedure for determinations of enzymatic activity could be used for large-scale screening of newborns. The protocols in this volume expand both the breadth and depth of readily available methods for quantitative proteomic researchers using MS. .
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 | R7148 | Radical-Mediated Protein Oxidation from Chemistry to Medicine | This unique and comprehensive volume brings together up-to-date information on the oxidation of amino acids, peptides, and proteins by free radicals and discusses the roles of such processes in biological systems. It pulls together aspects of protein oxidation from diverse fields: medicine, chemistry, biochemistry and contains an extensive bibliography drawing together thousands of references.
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| | Z374245 | Regulatory Protein Modification: Techniques and Protocols Neuromethods, Vol. 30 | | pricing |
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 | Z705233 | Separation Methods in Proteomics | Protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. This book provides an examination of all major separation techniques for proteomics research. A compilation of hands-on methods, it may serve as a guide for selection of the optimal separation strategies to solve particular biological problems. Progress in the development of robust analytical techniques and instrumentation has created the need for good quality biological samples that are subject to analysis. Emphasizing the importance of sample preparation, the book explains how proteomes can be divided into smaller, less complicated "subproteomes" for individual analysis.
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 | Z701653 | Short Protocols in Protein Science | "Short Protocols in Protein Science" provides condensed descriptions of more than 500 protocols compiled from "Current Protocols in Protein Science". Drawing from both the original "core" manual as well as the quarterly update service, this compendium includes all step-by-step descriptions of the principal methods covered in "Current Protocols in Protein Science".
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| | Z371939 | Spectroscopic Methods for Determining Protein Structure in Solution | A wide-ranging primer on spectroscopic methods for protein chemists. Covers circular dichroism, UV, IR, NMR, and Raman spectroscopy: theoretical background, what information each technique can provide, literature references, and how to combine data from several techniques to thoroughly elucidate protein structure.
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 | Z700533 | Structural Bioinformatics | This text provides an understanding of the theories, algorithms, resources, and tools used in structural bioinformatics. Reader emerges with the ability to make effective use of protein, DNA, RNA, carbohydrate, and complex structures to better understand biological function. It draws a clear connection between structural studies and the rational design of new therapies. This text covers the theory, biology, resources, and practical applications for computational approaches to biomolecular structural studies and features editors who have made significant contributions to the Protein Data Bank, the central repository for structural information.
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 | S1812 | Structure and Mechanism in Protein Science | There is a new era of protein design, sparked by the convergence of protein folding and enzymology. This book is a depiction of the principles of protein structure, activity, and mechanism today. It takes a more general look at mechanisms in protein science, emphasizing the unity of concepts in folding and catalysis and the importance of the relationships between basic chemistry, kinetics, thermodynamics, and structure. It makes protein engineering easier to understand and apply.
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| | Z370169 | Structure in Protein Chemistry | A comprehensive treatment of aspects of protein structure, building from the electronic structure of amino acids through the secondary and tertiary structure of domains, to the assembly of oligomers from subunits. Views on structural features are supplemented with detailed discussions of the methods used to elucidate them. Extensive reference lists accompany each chapter.
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 | Z374180 | Techniques in Protein Chemistry VII | This bench-top reference highlights current methods for protein design and engineering, protein sequencing, protein folding, and amino acid analysis. Included in this book is information on mass spectral analysis of peptides and proteins and high-sensitivity protein and peptide separations.
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| | Z364444 | Techniques in Protein Modification | Encyclopedic coverage of chemical methods for site-specific protein modification and methods for characterizing modified proteins. Appendices list commonly used reagents and preferred suppliers, and a discussion of general protein assay methods.
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 | Z704989 | Ten Most Wanted Solutions in Protein Bioinformatics | The book considers the ten most significant problems for those looking to identify the biological properties and functional roles of proteins. Problem One considers the challenge involved with detecting the existence of an evolutionary relationship between proteins. Two and Three studies the detection of local similarities between protein sequences and analysis in order to determine functional assignment. Four, Five, and Six look at how the knowledge of the three-dimensional structures of proteins can be experimentally determined or inferred, and then exploited to understand the role of a protein. Seven and Eight explore how proteins interact with each other and with ligands. Nine moves us out of the realm of observation to discuss the possibility of designing completely new proteins tailored to specific tasks. Ten considers ways to modify the functional properties of proteins.
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 | Z704539 | The Ribosome: Protein Manufacturer, An Introduction | This book offers an economically sound resource for those who are interested in ribosomes and translation. It is the first book that integrates the synthetic life work, structural findings, regulation and evolution of ribosomes written from a single voice perspective. The history of ribosomal research and the known translational exceptions of the Genetic Code are included. Moreover, this lecture makes a concerted effort at incorporating newer structural information, so that it actually leads to a further and better understanding of biological translation.
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