Our RNA Interference (RNAi) books offer scientists information on one of the hottest tools in research biology. With our books, scientists can assess the function of genes by turning them off and seeing what happens to the cell.
RNA Interference (RNAi): Nuts & Bolts of RNAi Technology
This book offers protocols and advice for the use of RNA interference for knocking down expression of target genes in a number of different eukaryotes. Varied applications in mammalian systems are explored in depth by authors currently working at the leading edge of RNA intererence methodologies. Chapters include: Design, synthesis and preparation of synthetic RNAi; Expression of RNAi from recombinant DNA and viral vectors; High-throughput RNAi applications; RNAi in mammalian systems.
This hand-on guide to RNA interference makes the power of targeted gene silencing available to any laboratory with a basic equipment for the handling of nucleic acids. In easy to follow step-by-step protocols you will learn: * How RNAi works in plants, flies and mammals * How to design the most efficient RNAi constructs * How to achieve transient, stable and conditional RNAi in cell cultures * How to determine the efficiency of an RNAi experiment * How to use RNAi for gene therapy. All protocols have been thoroughly tested in the author's laboratory. Examples of successful experiments and troubleshooting hints are provided to help in establishing your own successful RNAi experiments.
RNA Interference, Editing, and Modification: Methods and Protocols
A comprehensive collection of cutting-edge methods for elucidating the function of new genes and altering gene expression. These readily reproducible techniques can be used either in transient and stable gene splicing applied to worms, flies, trypanosomes, mammals, and plants, or in studying RNA editing mechanisms in a wide range of organisms, including systems that involve the conversion of one base to another and insertion/deletion editing. Topics of interest include stable and transient RNA interference, gene silencing, RNA editing, bioinformatics, small noncoding RNAs, and RNomics.
A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. Protocols follow the Methods in Molecular Biology series format.
The use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific, remarkably potent, and acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and cheaper way of mimicking the effects of mutations in both cell cultures and in living animals. This book presents the principles of RNA interference and reliable protocols for its laboratory use in C. elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more.
The use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific, remarkably potent, and acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and cheaper way of mimicking the effects of mutations in both cell cultures and in living animals. This book presents the principles of RNA interference and reliable protocols for its laboratory use in C. elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more.
In this edition, hands-on experts take advantage of the latest advances in ribozyme, DNAzyme, and RNA interference technologies to describe in detail the successful methods available for gene inactivation in vitro and in vivo. Their optimized techniques employ hairpin ribozymes, DNAzymes, hammerhead ribozymes and derivatives, group I intron ribozymes, Rnase P ribozymes, and siRNAs, as well as general methods for RNA structure analysis, delivery of oligonucleotides, and gene therapy. Also provided are novel methods for identifying accessible cellular mRNA sites; group I intron and RNAse P ribozymes protocols for effective design, selection, and therapeutic applications; and the latest RNAi methods for sequencing-specific gene silencing in a wide variety of organisms.
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