Animal Cell Biotechnology: Methods and Protocols, 2nd ed.
This second edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation. Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques.
The 12th edition features the same comprehensive, authoritative content and adds new and updated material throughout. Detailed hands-on procedures make the content more interesting and relevant by describing exactly what takes place in the micro lab. There are 3 new chapters along with general considerations and applications of information provided in Bacterial Sections of Part III which explains the criteria for organism inclusion and how it should be used. This guide to Part III includes gram reaction, shape, arrangement, atmosphere preferred, oxidase and catalase reactions, among other decision points for various pathogens, creating a visual method of identifying and cross referencing organisms.
In the new edition of Biochemistry, instructors will see the all the hallmark features that made this a consistent bestseller for the undergraduate biochemistry course: exceptional clarity and concision, a more biological focus, cutting-edge content, and an elegant, uncluttered design. Accomplished in both the classroom and the laboratory, coauthors Jeremy Berg and John Tymoczko draw on the field’s dynamic research to illustrate its fundamental ideas.
Biochemistry of Signal Transduction and Regulation, 4th ed.
This all-new edition of a best-selling text has been thoroughly updated to keep pace with the rapid progress in signal transduction research. With skill and clarity, the molecular basis of signal transduction, regulated gene expression, the cell cycle, tumorigenesis and apoptosis is made transparent for everyone with a basic knowledge in biochemistry or molecular biology. "The clear and didactic presentation makes it a textbook very useful for students and researchers not familiar with all aspects of cell regulation." (Biochemistry) "This book is actually two books: Regulation and Signal Transduction." (Drug Research)
Blood Banking and Transfusion Medicine: Basic Principles and Practice
Ever since the discovery of blood types early in the last century, transfusion medicine has evolved at a breakneck pace. This second edition of Blood Banking and Transfusion Medicine is exactly what you need to keep up. It combines scientific foundations with today's most practical approaches to the specialty. From blood collection and storage to testing and transfusing blood components, and finally cellular engineering, you'll find coverage here that's second to none. New advances in molecular genetics and the scientific mechanisms underlying the field are also covered, with an emphasis on the clinical implications for treatment. Whether you're new to the field or an old pro, this book belongs in your reference library.
Cancer and the Search for Selective Biochemical Inhibitors, 2nd ed.
The Second Edition delineates the underlying biochemical principles for alternative cancer therapies. Completely revised and updated, this edition includes coverage of the link between concepts and practices of alternative and conventional medicine. The author examines anticancer plant substances and other alternatives such as Vitamin C, essiac tea, shark cartilage, and cat’s claw. The text also addresses the problem of determining selective and non-toxic enzyme inhibitors for cancer cell metabolic pathways.
With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.
An exceptionally lucid guide to the latest immunology concepts! Through five best-selling editions, readers worldwide have appreciated this book's in-depth yet straightforward, richly illustrated approach to this complex subject. Now, sweeping updates incorporate the latest discoveries about innate immunity, the organization of lymphoid organs, and many other cutting-edge topics. Plus, online access via STUDENT CONSULT makes this an even more powerful learning resource!
Columns for Gas Chromatography: Performance and Selection
Gas Chromatography (GC) is the most widely used method for separating and analyzing a wide variety of organic compounds and gases. There have been many recent advancements in both packed column and capillary column GC. With numerous options and considerations, selecting the right column can be complicated. This resource includies - Methods of choosing both capillary and packed columns; Selection of dimensions (column length, I.D., film thickness, etc.) and type of column; Guidelines for proper connections of the column to the injector and detector; United States Pharmacopeia and National Formulary chromatographic methods; ASTM, EPA, NIOSH, and OSHA column selection specifications; Information on the advantages of computer assistance in GC and multidimensional GC; Comprehensive information on column oven temperature control
Comprehensive Organic Reactions in Aqueous Media 2nd ed.
An extensive update of the classic reference on organic reactions in water. More than forty percent of this extensively updated second edition covers new reactions. For ease of reference, it is organized by functional groups. A core reference: * Provides the most comprehensive coverage of aqueous organicreactions available; * Covers the basic principles and theory and progresses to applications; * Provides examples of applications in industry; * Includes alkanes, alkenes, aromatics, electrophilic substitutions, carbonyls, alpha, beta-unsaturated carbonyls, carbon-nitrogen bonds, organic halides, pericyclic reactions, photochemical reactions, click chemistry, and multi-step syntheses?
From the leading branded source for methods in the life sciences, this bookprovides every researcher with the skills and understanding of fundamental laboratory procedures to ensure greater success at the bench. It takes the novice researcher from the very basics of reagent preparation to the use of routine instrumentation found in most life science research laboratories around the world. This text teaches new investigators how to perform basic laboratory research techniques, describes solution chemistry and preparation, and covers basic laboratory safety. It also describes care and use of common equipment such as pH meters, spectrophotometers, centrifuges, and microscopes, and teaches how to manage information from lab notebooks, images, literature references, as well as manuscript preparation
This abridged version of the bestselling reference Handbook of Stem Cells, Two-Volume Set attempts to incorporate all the essential subject matter of the original two-volume edition in a single volume. The material has been reworked in an accessible format suitable for students and general readers interested in following the latest advances in stem cells, including full color presentation throughout. Although some extra language and chapters have been deleted, rigorous effort has been made to retain from the original two-volume set the material pertinent to the understanding of this exciting area of biology. The organization of the book remains largely unchanged, combining the prerequisites for a general understanding of adult and embryonic stem cells; the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations; as well as a presentation by the world's experts of what is currently known about each specific organ system.
Expression and Analysis of Recombinant Ion Channels
The latest developments are covered by editors and authors working for major pharmaceutical companies and who routinely apply these techniques in their daily work. The first three chapters cover the use of the Xenopus oocyte expression system for structure-function studies, from basic approaches for manipulating ion channel cDNAs to more specialized but powerful techniques. This is followed by reviews of strategies and methodologies available for expressing channels in mammalian cells and for their analysis by patch-clamp electrophysiology. Chapters 6 to 8 review the latest methodologies for ion channel drug discovery, including high throughput screening using fluorescence and luminescence, as well as automated planar array electrophysiology.
Genetic Variation: A Laboratory Manual (pb) Paperback
This manual is the first compendium of protocols specifically geared towards genetic variation studies, and includes thorough discussions on their applications for human and model organism studies. It covers the complete spectrum of genetic variation-from SNPs and microsatellites to more complex DNA alterations, including copy number variation. The early sections of the manual are devoted to study design and generating genotype data, the use of resources such as HapMap and dbSNP, as well as experimental, statistical, and bioinformatic approaches for analyzing the data. The final sections include descriptions of genetic variation in model organisms and discussions of recent insights into human genetic ancestry, forensics, and human variation.
Handbook of Functional Neuroimagery of Cognition 2nd ed.
This second edition has been completely updated to reflect new developments in the field, with existing chapters rewritten and new chapters added to each section. The section on history and methods now includes a chapter on the crucial topic of the physics of functional neuroimaging; the chapters on skill learning and executive functions are new to the domain section; and chapters on childhood development and emotion and social cognition have been added to the section on developmental, social, and clinical applications. The color insert has been increased in size, enhancing the visual display of representative findings.
The Second Edition compiles the latest data and provides hard evidence on the prophylactic and medicinal properties of many natural foods. This handbook reviews more than 200 nutraceutical compounds. Each chapter includes the chemical properties, biochemical activity, dietary sources, and evidentiary findings for each compound. New topics include the use of exopolysaccharides from lactic acid bacteria, protein as a functional ingredient for weight loss, and nutraceuticals to be used in the adjunctive treatment of depression. Two new chapters discuss recent evidence on oxidative stress and the antioxidant requirements of athletes as well as the use of nutraceuticals for inflammation. The scientific investigation of nutrition and lifestyle changes on the pain and debilitation of osteoarthritis is the subject of another new article.
In Vitro Transcription and Translation Protocols 2nd ed.
This Second Edition provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration and of continuous-exchange devices to obtain high levels of in vitro protein production, the efficient in vitro production of integral membrane proteins, a protein category known to be extremely resistant to in vivo expression, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. Other chapters also discuss the efficient, high-throughput protein production using the wheat germ in vitro expression system and exogenous protein expression in xenopus oocytes. Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy and to follow the surge of drug resistance, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the use of in vitro transcription and translation to directly identify proteins in 2D-maps. Additional chapters investigate high-throughput in vitro expression of Bacillus anthracis antigens to identify vaccine candidates, the use of Xenopus laevis translation system to study the functional expression of type 1 rat GABA transporter, and the exploitation of wheat germ in vitro transcription and translation system for the production of highly purified proteins amenable to NMR studies.
The seventh edition guides the reader through the immune system in all its aspects - from the first engagement of innate immunity to the generation of the adaptive immune response and its clinical consequences. It has been comprehensively updated throughout, and includes new information on topics such as NK cells, Toll-like receptors, AID, viral evasins, mucosal immunity, and celiac disease, to name a few. Each copy of the book includes a revised CD-ROM, Immunobiology Interactive, which contains animations and videos with voiceover narration, as well as the figures from the text for presentation purposes.
Mass Spectrometry: Principles and Applications 3rd ed.
The latest edition provides a complete overview of the principles, theories and key applications of modern mass spectrometry. All instrumental aspects of mass spectrometry are clearly and concisely described: sources, analysers and detectors. Tandem mass spectrometry is introduced early on and then developed in more detail in a later chapter. Emphasis is placed throughout the text on optimal utilisation conditions. Various fragmentation patterns are described together with analytical information that derives from the mass spectra. Updates include: Increased coverage of MALDI and ESI, more detailed description of time of flight spectrometers, new material on isotope ratio mass spectrometry, and an expanded range of applications.
The 18th Edition features a wealth of new content and greater ease of use. • New clinical approach chapters • New disease summaries • 34 new chapters and 69 new illustrations • New cross-referencing to specific pages • New two-color printing • Written by more than 300 medical experts in various fields of medicine • All text has been peer reviewed
For nearly a quarter century Molecular Biology of the Cell has been the leading cell biology textbook. This tradition continues with the new Fifth Edition, which has been completely revised and updated to describe our current, rapidly advancing understanding of cell biology. To list but a few examples, a large amount of new material is presented on epigenetics; stem cells; RNAi; comparative genomics; the latest cancer therapies; apoptosis (now its own separate chapter); and cell cycle control and the mechanics of M phase (now integrated into one chapter). The hallmark features of Molecular Biology of the Cell have been retained, such as its consistent and comprehensive art program, clear concept headings, and succinct section summaries. Additionally, in response to extensive feedback from readers, the Fifth Edition now includes several new features. It is now more portable. Chapters 1-20 are printed and Chapters 21-25, covering multicellular systems, are provided as pdf files on the free Media DVD-ROM which accompanies the book.* And for the first time, Molecular Biology of the Cell now contains end-of-chapter questions. These problems, written by John Wilson and Tim Hunt, emphasize a quantitative approach and the art of reasoning from experiments, and -they will help students review and extend their knowledge derived from reading the textbook. The Media DVD-ROM, which is packaged with every copy of the book, contains PowerPoint® presentations with all of the figures, tables and micrographs from the text (available as JPEGs too). Also included is the Media Player, which plays over 125 movies—animations, videos, and molecular models—all with voiceover narration. A new reader-friendly feature is the integration of media codes throughout the text that link directly to relevant videos and animations. The Media DVD-ROM holds the multicellular systems chapters (21-25) of the text as well. By skillfully extracting the fundamental concepts from this enormous and ever-growing field, the authors tell the story of cell biology, and thereby create a coherent framework through which readers may approach and enjoy this subject that is so central to all of biology.
This book provides, for the first time ever, a completely integrated look at chemistry, biology, drug discovery, and medicine. This book delves into the discovery, application, and mode of action of more than one hundred of the most significant molecules now used in modern medicine. With focus on the most important and commonly used medicines such as anti-clotting agents, antibiotics, Viagra and anti-malarial and antidepressant drugs, coverage includes the condition it treats, the summary of its industrial development, the year put into practice, the biological target for the medicine, the human proteins related to the disease, the metabolism or action of the condition treated, and interacting side effects and related drugs.
With the second edition, the author has once more provided a unique, self-contained introduction to the physical concepts, techniques and applications of nanoscale systems by covering its entire spectrum from the latest examples right up to single-electron and molecular electronics. New sections have been added on the use of DNA as an organizing stratagem in self-assembly, silicon nanowires, comments on the new success toward human cloning, the achievement of self-replication in a primitive set of electromechanical robots, recognition in the extra chapters of the acceleration toward alternative forms of nanoelectronics.
In this book authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome scale experiments and for small scale individual PCR amplifications. This volume will be useful for organizations perfomring whole genome studies, companies designing instruments that utilize PCR, and individual scientists who routinely use PCR in their research.
PET Chemistry: The Driving Force in Molecular Imaging
The incessant progress in the comprehension of molecular processes of disease formation and progression is accompanied by an increasing importance of biomedical molecular imaging methods. PET, a nuclear medical imaging modality, is ideally suited to produce such kinds of images by use of positron-emitting chemical entities. The purpose of this workshop was to discuss and overview applications and technologies in the area of PET chemistry. The book provides the reader with state-of-the-art information on the different applications of PET isotopes, such as F-18, C-11, Ga-68, Cu-64, Y-86, Br-76, Br-77 and I-124, and their impact on molecular imaging. New technical features such as microwaves, microreactors and modules for the synthesis of new PET tracers are discussed.
This book is a compendium of cutting-edge protocols for quantitative proteomics, and presents the most significant methods used in the field today. The focus on mass spectrometry (MS) is integral, as MS has, and will continue to be, an essential tool in proteomics for studying complex biological systems and human diseases. This volume, written and compiled by leading quantitative proteomic experts, is an indispensable resource in the search for novel biomarkers. Quantitative Proteomics by Mass Spectrometry presents several innovative MS quantitative procedures, including a variety of methods for introducing isotopic labels and quantifying post-translational modifications. Some of these methods include growing an organism in isotope-enriched media, performing trypsin proteolysis in the presence of 18O-water, reacting protein samples with isotopically labeled reagents, quantifying relative amount of proteins without the use of any isotopic labels. Attention is also given to state-of-the-art techniques for the characterization of the phosphoproteome and tandem MS for detection of inborn errors of metabolism. Specifically, the procedure for determinations of enzymatic activity could be used for large-scale screening of newborns. The protocols in this volume expand both the breadth and depth of readily available methods for quantitative proteomic researchers using MS. .
Regulatory RNAs” was the theme of the 71st annual Cold Spring Harbor Symposium on Quantitative Biology, where scientists from around the world presented the latest advances in the biology of RNAs, including RNA interference, transcriptional and translational control, RNA editing, and the role of RNAs in biological circuits and epigenetic events. Investigators discussed the latest technologies aimed at large-scale characterization of RNAs, as well as the roles of small RNAs in development and cancer. This volume is a timely survey of this important new area of molecular biology.
As molecular and cellular biologists move toward nano–techniques for performing experiments on single molecules rather than on populations of molecules, a comprehensive manual on how (and why) to carry out such experiments is needed. Single-Molecule Techniques: A Laboratory Manual fills this requirement—it is the first to take researchers who know nothing about single–molecule analyses to the point where they can successfully design and execute appropriate experiments. Geared toward research scientists in structural and molecular biology, biochemistry, and biophysics, the manual will be useful to all who are interested in observing, manipulating, and elucidating the molecular mechanisms and discrete properties of macromolecules. Techniques range from in vivo and in vitro fluorescent–based methods to the use of atomic force microscopy, optical and magnetic tweezers, and nanopores. The book is edited by Paul R. Selvin and Taekjip Ha, two pioneers in the field of experimental biophysics who have made significant contributions to the development and application of single–molecule technologies.
This unique resource brings scientists up to date on recent advances and provides the information they need to select candidate adjuvants. Written by leading experts, this book provides a comprehensive overview of the rapidly evolving field and developing formulation methods and covers cutting-edge technologies and gives the current status of adjuvants in clinical trials and those still in the pre-clinical stage. It includes detailed information on specific vaccine adjuvants, including MF59, TLR4 agonists, new iscoms, cytokines, polyphosphazenes, and more. The text discusses the mechanisms of adjuvant actions, and covers some novel adjuvants and delivery systems and the safety evaluation of adjuvants
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