A to Z of DNA Science: What Scientists Mean When They Talk about Genes and Genomes
Medical science constantly demands our attention, as patients or relatives, concerned citizens, voters, investors, or simply curious individuals. But for those without training, the language of science is often hard to follow. The A to Z of DNA Science book series defines and illustrates specialized terms in ways that non-specialists can appreciate and enjoy. This volume focuses on the language of genes, genomes, DNA, biotechnology, and heredity, defining, explaining, and illustrating over 200 terms used in books, broadcasting, websites, and newspaper and magazine articles
A to Z of DNA Science: What Scientists Mean When They Talk about Genes and Genomes
Medical science constantly demands our attention, as patients or relatives, concerned citizens, voters, investors, or simply curious individuals. But for those without training, the language of science is often hard to follow. The A to Z of DNA Science book series defines and illustrates specialized terms in ways that non-specialists can appreciate and enjoy. This volume focuses on the language of genes, genomes, DNA, biotechnology, and heredity, defining, explaining, and illustrating over 200 terms used in books, broadcasting, websites, and newspaper and magazine articles.
Abraham Lincoln’s DNA and Other Adventures in Genetics
Twenty-four true, wide-ranging tales of crime, history, human behavior, illness, and ethics, told from the personal perspective of the author, an eminent physician-lawyer who uses the stories to illustrate the principles of human genetics and to discuss the broader issues.
Ageless Quest: One Scientist’s Search for the Genes That Prolong Youth
Ageless Quest is a personal, sometimes controversial, account of the pursuit of a genetic ‘cure’ for aging by an expert in the field. The author is the Novartis Professor of Biology at the Massachusetts Institute of Technology. Aging has always been regarded as a highly complex process with many degenerative changes leading to the cessation of life. But recent research has identified a relatively simple mechanism that governs the pace of aging. Lenny Guarente's Ageless Quest is a scientific detective story for the baby boom generation. It offers an insider's view of an area of potentially astonishing high reward and equally high risk.
A genome revolution is transforming biomedical research in the leading laboratories. Now, a series of manuals from Cold Spring Harbor puts the tools of the revolution in everyone's hands. Assuming only a basic knowledge of molecular biology, these manuals explain how to clone, manipulate, analyze, and sequence large segments of DNA, and relate expressed sequence to phenotypic variation. The techniques are written for application to animal DNA as well as human genomes. They deal plainly with sources of failure - and solutions. Assembled by experienced CSH course instructors, the protocols are written by experts, often the methods' creators, and have been rigorously edited to Cold Spring Harbor standards of accuracy, consistency, and completeness. A complement to the bible of recombinant DNA, Molecular Cloning, these manuals are essential for every laboratory in which genes are being studied.
This book places the production and use of polyclonal and monoclonal antibodies within the reach of every biologist. All of the techniques described are supplemented with extensive notes and two-color illustrations.
The cruciferous weed Arabidopsis thialiana has become a model system for the study of an unusually wide variety of aspects of plant biology. As a result, nearly all aspects of Arabidopsis biology are now under investigation, more scientists are involved, and plant science as a whole has been invigorated. This book is the first comprehensive account of what is known about the organism. The information is presented in the context of plant biology in general, with the properties of Arabidopsis mutants and the insights derived from their analysis as a unifying theme. The book's scope includes genetics, growth and development, biochemistry, physiology, and responses to pathogens and environmental stress. This unique volume, in the classic tradition of the Cold Spring Harbor Monograph Series, is a landmark in plant science, essential reading for investigators at graduate student level and beyond, and a work of reference that will serve the field for years to come.
The thale cress Arabidopsis thaliana is increasingly popular among plant scientists. It is small, easy to grow, makes flowers, and the sequence of its small and simple genome was recently completed. This is the most complete and authoritative laboratory manual to be published on this model organism and the first to deal with genomic and proteomic approaches to its biology.
Newly appointed principal research investigators have to recruit, motivate, and lead a research team, manage personnel and institutional responsibilities, and compete for funding, while maintaining the outstanding scientific record that got them their position. No wonder that many principal investigators feel ill-prepared. In this book, a successor to her best-selling manual, “At The Bench”, Kathy Barker provides a guide for leaders of research teams, and those who aspire to that role. With extensive use of interviews and a text enlivened with quotes and real-life examples, she discusses a wide range of management challenges and skills that promote success.
At the Bench: A Laboratory Navigator, Updated Edition
At the Bench is the unique and hugely successful handbook for living and working in the laboratory, an essential aid to understanding basic lab techniques and how research groups work at a human level. In this newly revised edition, chapters have been rewritten to accommodate the impact of computer technology and the Internet, not only on the acquisition and analysis of data, but also on its organization and presentation. Alternatives to the use of radiation have been expanded, and figures and illustrations have been redrawn to reflect changes in laboratory equipment and procedures.
Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manuals Cells: A Laboratory Manual and Live Cell Imaging provide a solid course in the basics of using the microscope in a biology laboratory. This book provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events.
Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manuals Cells: A Laboratory Manual and Live Cell Imaging provide a solid course in the basics of using the microscope in a biology laboratory. This book provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events.
This handbook offers a practical guide to the principles of quantitative analysis in biological experiments. The material is primarily aimed at working molecular biologists, but the scope and clarity of presentation make it equally suitable for students. Topics covered range from the basics—such as measuring the concentrations of macromolecules—through considerations of binding constants and the kinetics of molecular interactions. The book ends with a thorough consideration of data analysis.
Application of computational methods to DNA and protein science is an exciting development. This book features sequence alignment, structure prediction, phylogenetic and gene prediction, database searching, and genome analysis that are clearly explained and illustrated along with underlying algorithms and assumptions. Theoretical underpinnings are linked to biological problems and solutions. Extensive tables provide descriptions and Web sources for a range of available software. An associated Website, accessible free of charge by book purchasers, provides links to Internet sources referred to in the text (http://www.BioinformaticsOnline.org).
The application of computational methods to DNA and protein science is a new and exciting development in biology. Bioinformatics: Sequence and Genome Analysis is a comprehensive introduction to this emerging field of study. The book has many unique and valuable features: It is written for any biologist who wants to understand methods of sequence and structure analysis and how the necessary computer programs work Sequence alignment, structure prediction, phylogenetic and gene prediction, database searching, and genome analysis are clearly explained and amply illustrated Underlying algorithms and assumptions are clearly explained for the non-specialist Examples are presented in simple numerical terms rather than complex formulas and notation Theoretical underpinnings are linked to biological problems and their solutions Extensive tables provide descriptions and Web sources for a broad range of publicly available software. Based on the author's extensive experience as a molecular geneticist and bioinformaticist at the University of Arizona, this is a uniquely educational book, ideal as a laboratory reference for investigators and also as teaching reference for graduate and undergraduate students studying this fast-changing discipline.
Bioinformatics: Sequence and Genome Analysis, 2nd Edition
As more species' genomes are sequenced, computational analysis of these data has become increasingly important. The second edition of this widely praised textbook provides a comprehensive and critical examination of the computational methods needed for analyzing DNA, RNA, and protein data, as well as genomes. New features include chapter guides and explanatory information panels and glossary terms. New chapters cover statistical analysis of sequence alignments, computer programming for bioinformatics, and data management and mining. Practically oriented problems at the ends of chapters enhance the value of the book.
Bioinformatics: Sequence and Genome Analysis, 2nd Edition
As more species’ genomes are sequenced, computational analysis of these data has become increasingly important. The second, entirely updated edition of this widely praised textbook provides a comprehensive and critical examination of the computational methods needed for analyzing DNA, RNA, and protein data, as well as genomes. The book has been rewritten to make it more accessible to a wider audience, including advanced undergraduate and graduate students. New features include chapter guides and explanatory information panels and glossary terms. New chapters in this second edition cover statistical analysis of sequence alignments, computer programming for bioinformatics, and data management and mining. Practically oriented problems at the ends of chapters enhance the value of the book as a teaching resource. The book also serves as an essential reference for professionals in molecular biology, pharmaceutical, and genome laboratories.
Living cells have evolved many ways of coping with metabolic events and environmental influences that damage DNA. These mechanisms, and the frequent progression to cancer that results when they go awry, are reviewed in this volume by authors from over sixty of the world’s leading laboratories. The topics discussed include DNA repair, mutagenesis and other damage-tolerance functions, checkpoint control, apoptosis, and adaptation. They draw from studies on human and yeast cells. Current, but with a valuable historical perspective, this volume has the depth and lasting value typical of this most prestigious series and is essential reading for investigators of DNA replication, cell cycle control, and tumorigenesis.
Living cells have evolved many ways of coping with metabolic events and environmental influences that damage DNA. These mechanisms, and the frequent progression to cancer that results when they go awry, are reviewed in this volume by authors from over sixty of the world’s leading laboratories. The topics discussed include DNA repair, mutagenesis and other damage-tolerance functions, checkpoint control, apoptosis, and adaptation. They draw from studies on human and yeast cells. Current, but with a valuable historical perspective, this volume has the depth and lasting value typical of this most prestigious series and is essential reading for investigators of DNA replication, cell cycle control, and tumorigenesis.
Studies of the cells and genes of the nematode C. elegans have become a cornerstone of current biology. A classic 1988 Cold Spring Harbor monograph, The Nematode Caenorhabditis elegans, described the basic genetics, anatomy and development of the organism. Now, in that authoritative tradition, comes C. elegans II -- not a second edition but a book that breaks new ground and defines the current status of the field, providing a detailed molecular explanation of how development is regulated and the nervous system specifies varied aspects of behavior. This volume is a must for any investigator doing worm studies but it has been written and rigorously edited to illuminate for a wider community of investigators in cell and molecular biology who should know how new knowledge of C. elegans relates to their own specialty.
Normal cardiovascular function requires the concerted action of many cell types, each capable of adaptive gene expression in response to developmental, physiological, and pathological cues. The genetic basis of cardiovascular function, development, and disease is an area of intense investigation, in the hope of significant insights into the heart and vessels? basic workings and improvements in diagnosis and therapy. This latest volume in a prestigious book series presents a remarkable survey of current progress in these efforts, through the contributions of over fifty of the world's leading investigators. Sections are devoted to angiogenesis, cardiogenesis, homeostasis, development, vascular biology, and cardiovascular repair and therapy. The book is an essential source of ideas, discoveries, and references for clinical scientists and physicians interested in basic cardiac biology, hypertension, atherosclerosis, coronary artery disease, and heart failure.
Normal cardiovascular function requires the concerted action of many cell types, each capable of adaptive gene expression in response to developmental, physiological, and pathological cues. The genetic basis of cardiovascular function, development, and disease is an area of intense investigation, in the hope of significant insights into the heart and vessels? basic workings and improvements in diagnosis and therapy. This latest volume in a prestigious book series presents a remarkable survey of current progress in these efforts, through the contributions of over fifty of the world's leading investigators. Sections are devoted to angiogenesis, cardiogenesis, homeostasis, development, vascular biology, and cardiovascular repair and therapy. The book is an essential source of ideas, discoveries, and references for clinical scientists and physicians interested in basic cardiac biology, hypertension, atherosclerosis, coronary artery disease, and heart failure.
This monograph, written by experts in the field, is devoted to the molecular analysis of addiction pathways in the brain. It provides an intensive overview of the fundamentals, state–of–the–art advances, and major gaps in the cell and molecular biology of drug addiction within the broader context of neuroscience. Addiction research is a branch of neuroscience and psychology. The emphasis in this book is on hard science and the market for it will be found among research investigators and grad students within the field of neuroscience. The research presented is not only applicable to the study of drug abuse and addiction, but has clear implications for clarifying mechanisms of learning and memory, neuroadaptation, perception, volitional behavior, motivation, reward, and other disciplines of neuroscience.
Recent breakthroughs in the field of cell growth, particularly in the control of cell size, are reviewed by experts in the three major divisions of the field: growth of individual cells, growth of organs, and regulation of cell growth in the contexts of development and cell division
Recent breakthroughs in the field of cell growth, particularly in the control of cell size, are reviewed by experts in the three major divisions of the field: growth of individual cells, growth of organs, and regulation of cell growth in the contexts of development and cell division. This book is an introductory overview of the field and should be adaptable as a textbook.
Cells of the Body: A History of Somatic Cell Genetics
This book is the first scholarly history of research into the genetics of body cells, from its origins in the 19th century to the present day. Henry Harris, a well-known writer and a distinguished investigator in cell biology and cancer genetics, brings an unusually informed perspective to the technical aspects of his subject. He has written a book to be enjoyed not just by professional historians of science, but by working scientists in genetics, cell biology, and cancer research, from the graduate student level upwards. Its readers will derive a richer understanding of how and why the cells of the body are studied in the way that they are today.
A concise, readable account of the principles underlying embryonic and appendage development in animals and plants, illustrated with colorful artwork and interviews with prominent investigators. Highly recommended for use in undergraduate and graduate courses.
A concise, readable account of the principles underlying embryonic and appendage development in animals and plants, illustrated with colorful artwork and interviews with prominent investigators. Highly recommended for use in undergraduate and graduate courses.
Correcting the Blueprint of Life: An Historical Account of the Discovery® of DNA Repair Mechanisms
In this brief, readable, and revealing book, one of the pioneers of the now rapidly evolving field of DNA repair traces the history of the discovery of the more important mechanisms by which cells respond to DNA damage. Errol Friedberg has written an enjoyable and informative introduction to the study of DNA mutagenesis and re-pair that will interest students at an advanced undergraduate or graduate student level as well as investigators in fields as diverse as oncogenesis, cell cycle regulation, transcription and DNA replication.
DNA from the Beginning (a 3–CD set) is a multimedia primer on the basics of DNA, genes, and heredity. It is an ideal teaching tool for high school students and will also interest adults with a non-technical background who wish to learn more about the impact that DNA science is having on our lives. The content in DNA from the Beginning is built on a progressive series of important but simply stated concepts such as Children resemble their parents. An understanding of the concept is then conveyed in clear,understandable terms with the aid of links to animations, photographs, and video clips, and biographies or interviews featuring key scientists. A wealth of other information and links to web resources are included. Problems and their solutions are included to allow teaching through self-examination. Based on the award-winning web site, the DNA from the BeginningCD–ROM set provides over 72 hours of study time. It runs on Macintosh computers and PCs, and gives immediate access to all the online content, in attractive, uncluttered screens, without the need for network connections.
DNA Interactive (DNAi) is a multimedia resource for high school and introductory college biology that shows how we came to understand the language of DNA, how we bend it to our own service, and what it can tell us about who we are. The DNAi products an award winning website (http://www.dnai.org) and the DVDare the result of a two year collaboration between scientists, educators, and film makers on three continents. It includes more than 200 video clips and animations; video interviews with 11 Nobel Laureates and more than 50 other scientists, clinicians, and patients; and spectacular 3 D animations that illustrate intracellular processes and explore aspects of DNA science.
DNA microarray, or DNA chip technology is a new and powerful means of discovering, characterizing and analyzing genes and their expression patterns. This manual assembles and synthesizes the expertise of over 30 innovators in this emerging field to provide authoritative, detailed instruction on the design, construction, and applications of microarrays, as well as comprehensive descriptions of the software tools and strategies required for extensive image and data analysis. It complements the best selling manual "Molecular Cloning".
DNA microarray technology is a new and powerful means to analyze genomes and characterize patterns of gene expression. Its applications are widespread across the many fields of plant and animal biological and biomedical research. This manual, designed to extend and to complement the information in the bestselling Molecular Cloning, is a synthesis of the expertise and experience of more than 30 contributors - all innovators in a fast-moving field. DNA Microarrays provides authoritative, detailed instruction on the design, construction, and applications of microarrays, as well as comprehensive descriptions of the software tools and strategies required for analysis of images and data.
An international panel of contributors combine to create this exhaustive treatise. General topics include origins of replication, priming, role of transcription factors in replication, fidelity and repair, chromatin structure, and comparisons to prokaryotic mechanisms. A section on proteins includes chapters on cellular and viral DNA polymerases, accessory proteins, ligases, topoisomerases, and telomerases. Finally, about half the book is devoted to discussion of specific replication systems including eleven viruses, yeast, insects, plants, mammals, organelles, and more.
Davenport’s Heredity in Relation to Eugenicsd: A View from the 21st Century
When Charles Davenport published Heredity in Relation to Eugenics in 1911, he advanced his ideas of where genetics would go in the rest of the 20th century. In this volume a group of scientists take some of Davenport's themes - mental illness, human evolution, nature and nurture - and discuss them in a 21st century context. Davenport's original books is reprinted along with the assays.
The idea of human gene therapy was accepted by the medical community and society at large long before believable clinical benefits began to emerge. In this book, some of the field’s most distinguished contributors chronicle the evolution of this momentous direction for medicine, illustrating how imaginative concepts shaped the development of technologies and brought the daring new idea to its current position of imminent practical success. This is a book designed to endure as clinical advances accumulate, a clear-eyed work of reference that will anchor the further development of this revolution in therapy. It is an essential addition to libraries of clinical medicine, biotechnology, and public policy, and a resource that no laboratory investigator with an interest in the biology of gene transfer should be without.
Discovering Genomics, Proteomics, and Bioinformatics
This is the first textbook which combines integrated web exercises with a problem solving approach to train students in basic hands-on genomic analysis. The authors present global problems, then provide the tools of genomic analysis to help students dissect the answer. Microarray images and all art and figures from the text are available on the free CD-ROM. The Discovering Genomics, Proteomics, and Bioinformatics Companion Websitewww.geneticsplace.com helps one learn about cutting-edge research and visualize complex processes.
This workbook is a companion to Discovering Molecular Genetics: A Case Study Course with Problems and Scenarios. One of the unique features of the latter is: Over 140 problems, including scenarios from history, mythology, movies, and TV series, which test students' abilities to apply molecular genetic concepts. Solutions and strategies for these problems are provided in the companion workbook, which is also available separately.
Discovering Neurons: The Experimental Basis of Neuroscience
Discovering Neurons is a collection of investigative laboratory experiments designed for the advanced undergraduate and the first or second year graduate student. In its three major sections, neurons are studied as: individual cells, including topics such as intracellular recording, immunocytochemistry and HPLC forming connections, with experiments on tract tracing, information coding and LTP, among others as coordinators of behavior, through approaches such as pharmacology, lesioning and ethology. The fourth section focuses on techniques applicable to several experiments in the collection, including electrophysiology recording, surgery, anesthetics and staining methods. The experiments introduce students to the techniques used in today's leading neuroscience research labs. They have been thoroughly tested in the student laboratory and are written and designed to be easily integrated into existing neuroscience courses of any length or emphasis. This is a unique volume that is an important resource for all teachers of neuroscience.
The structure of DNA proposed by James Watson and Francis Crick in 1953 was one of the most important scientific discoveries of the twentieth century, transforming biology, giving medicine new impetus, and providing a foundation for the new industry of biotechnology. Jim Watson’s best-selling memoir of the events leading to this discovery, The Double Helix, has enthralled millions of readers since its publication in 1968. In this videotaped lecture, recorded live at Cold Spring Harbor Laboratory in November 1999, Dr. Watson retraces those events in a very personal reminiscence of the period and the people involved. This is an account of one of the great dramas of science, told from the intimate perspective of a participant who, aged 25 at the time of the discovery, has become one of the intellectual leaders of our time. James D. Watson was Director of Cold Spring Harbor Laboratory, New York, from 1968 to 1993 and is now its President. He was the first Director of the National Center for Human Genome Research of the National Institutes of Health from 1989 to 1992. Dr. Watson is a member of the National Academy of Sciences and Royal Society and has received many awards and honors, including the Presidential Medal of Freedom and the National Medal of Science. In addition to The Double Helix, his books include Molecular Biology of the Gene, Molecular Biology of the Cell, Recombinant DNA, and A Passion for DNA. PAL versions 60 minutes
"...the point of this story, I'll tell you right now. Did you ever sit down and think about how It is that each time a baby's born It's a baby -- not a rabbit or an ear of corn?" The answer is in DNA -- the stuff that genes are made of. Twin brothers Joel and Ira Herskowitz, physician and scientist, respectively, have come up with a new way to explain to children (ages 8) how it is that when a person's genetic message is "copied," the result is another human being -- not a rabbit or an ear of corn. Based on a song written by Joel Herskowitz while he was in medical school, the "Double Talking Helix Blues" is a book and audio tape presentation on the structure and function of DNA. The book includes the text for Joel's song, which is illustrated with spectacular paintings by Judy Cuddihy to provide an important visual component to the overall "lesson" on DNA. This is followed by a guide written by Ira Herskowitz to explain the scientific terms and concepts in the song; this section describes in clear terms what, for example, a chromosome, nucleus, and molecule are. Accompanying the book is a 12-minute audio tape with performances of the song by Joel and Ira. All in all, the "Double Talking Helix Blues" provides young readers with a wonderful introduction to concepts of cells and molecules, reproduction at the molecular level, and DNA and its structure. The target age group for this presentation is ages 8 and up, as well as teachers, scientists, and all those interested in molecular biology at its most basic level.
This exceptional laboratory manual describes procedures used for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation and rigorously edited for clarity and conciseness. The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development. Each method is introduced and illustrated with figures, tables, illustrations, and examples of data obtainable.
The second edition of the highly successful grey book Drosophila: A Laboratory Handbook, first published in 1989, has been completely updated to reflect advances in techniques and knowledge. The original format is maintained, providing a handy reference guide to most aspects of the biology of Drosophila. Each of the thirty-six chapters summarizes the present state of the subject, written with a historical perspective, but with an emphasis on the practical use of genetic and other methods. Extensively referenced.
Early Development of Xenopus laevis: A Laboratory Manual
Xenopus embryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopus development has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere.
Early Development of Xenopus laevis: A Laboratory Manual
Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopus development has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopus embryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere.
Eighth Day of Creation: The Makers of the Revolution in Biology (Expanded Edition)
Unavailable in the U.S. for many years, Horace Judson's remarkable lay history of molecular biology is now published in a new, expanded edition. The author has added new material on some of the principal figures involved, particularly Rosalind Franklin. Also included are a new Forward and an Afterword which sketches the further development of molecular biology into the era of recombinant DNA. No one active in current molecular genetics can fail to be informed and entertained by this extraordinary account of how it all began. Also highly recommended for students and interested lay people.
The regulation of gene expression in many biological processes involves epigenetic mechanisms. In this new volume, 24 chapters written by experts in the field discuss epigenetic effects from many perspectives. There are chapters on the basic molecular mechanisms underpinning epigenetic regulation, discussion of cellular processes that rely on this kind of regulation, and surveys of organisms in which it has been most studied. Thus, there are chapters on histone and DNA methylation, siRNAs and gene silencing; X-chromosome inactivation, dosage compensation and imprinting; and discussion of epigenetics in microbes, plants, insects, and mammals. The last part of the book looks at how epigenetic mechanisms act in cell division and differentiation, and how errors in these pathways contribute to cancer and other human diseases. Also discussed are consequences of epigenetics in attempts to clone animals..
Epigenetics: Symposia on Quantitative Biology, volume LXIX 2004 Hard cover
The importance of epigenetics regulation of gene expression, particularly in higher organisms is now clear and the 2004 Cold Spring Harbor Annual Symposium provided many new examples as well as insights into underlying mechanisms. The resulting volume - with over 60 papers from experts across the field - covers various aspects of nuclear organization and dynamics; genomic imprinting, chromosomal inactivation, and other examples of gene silencing; the histone and DNA modifications associated with these conditions; and the roles of RNA and protein regulators in establishing and maintaining these states.
Epigenetics: Symposia on Quantitative Biology, volume LXIX 2004 Soft cover
The importance of epigenetics regulation of gene expression, particularly in higher organisms is now clear and the 2004 Cold Spring Harbor Annual Symposium provided many new examples as well as insights into underlying mechanisms. The resulting volume - with over 60 papers from experts across the field - covers various aspects of nuclear organization and dynamics; genomic imprinting, chromosomal inactivation, and other examples of gene silencing; the histone and DNA modifications associated with these conditions; and the roles of RNA and protein regulators in establishing and maintaining these states.
Essentials from Cells: A Laboratory Manual (Laboratory Essentials on CD)
Published in 1998, the three-volume lab manual Cells contained a comprehensive collection of techniques for studying cellular structure and function. This new CD-ROM,Essentials from Cells: A Laboratory Manual, presents a selection from the book of methods with proven reliability that every investigator working with cellular systems should have available. Essentials from Cells: A Laboratory Manual provides precisely crafted, step-by-step protocols and essential background details in a readily accessible, searchable electronic format.
Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. They participate in activities as diverse as protein folding, leukocyte physiology, and microbial pathogenesis. Genetic defects in their assembly are at the root of a growing list of human diseases. Essentials of Glycobiology is an ideal gateway to the understanding of glycans, and is the first textbook approach to describing their biogenesis and function. Written and edited by glycobiologists with experience in both teaching and research, this volume will be invaluable for students and established investigators in developmental biology, cell biology, neuroscience, immunology, and biochemistry, who need a complete yet concise introduction to this burgeoning field.
Experimental Design for Biologists explains how to establish the framework for an experimental project, how to set up a system, design experiments within that system, and how to determine and use the correct set of controls. Separate chapters are devoted to negative controls, positive controls, and other categories of controls that are perhaps less recognized, such as "assumption controls," and "experimentalist controls." Furthermore, there are sections on establishing the experimental system, which include performing critical "system controls." Should all experimental plans be hypothesis-driven? Is a question/answer approach more appropriate? What was the hypothesis behind the Human Genome Project? The answers to these kinds of questions can be found in this book. It provides compelling lessons in framing an experimental question, establishing a validated system to answer the question, and deriving verifiable models from experimental data.
Fly Pushing: The Theory and Practice of Drosophila Genetics, 2nd Edition
A new edition of the classic handbook that has become a standard in the Drosophila field. The book is expanded to include topics in which classical genetic strategies have been augmented with new molecular tools. Included are such new techniques as homologous recombination, RNAi, new mapping techniques, and new mosaic marking techniques.
Gastrulation is the complex process of cell differentiation, following the division of a fertilised embryo, during which tissue layers are formed and the overall body plan is established. This book is the definitive guide to this vitally important period in embryonic development, providing authoritative and up to date information that includes the first comprehensive interspecies comparison, cell movements and patterning events, the roles of individual genes and gene families, and the evolution of gastrulation.
Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual
Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. This book provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response.
Genes & Signals analyzes gene regulation from a new perspective. The first chapter describes mechanisms found in bacteria, and two subsequent chapters discuss which of these is most highly exploited in higher organisms. A final chapter relates these molecular strategies to other enzymatic processes, including those involving kinases, RNA splicing enzymes, proteases, and others. A general theme emerges, one that proposes how a rather restricted set of signals and enzymatic functions has been used in evolution to generate complex life forms of different types.
Genes and Signals: Imposing Specificity on Enzymes by Recruitment
Many cellular functions require the orderly switching on and off of gene transcription, often in response to signals from other cells or the environment. This concise, attractively illustrated volume is a textbook on gene regulation and its importance in development and evolution. But the book also argues that understanding how transcription-related enzymes are controlled will reveal much about the regulation of enzymes in other biological processes as well.
Genetic Switch, Third Edition, Phage Lambda Revisited
The first edition of Mark Ptashne’s 1986 book describing the principles of gene regulation in phage lambda became a classic in both content and form, setting a standard of clarity and precise prose that has rarely been bettered. This newly updated third edition focuses once again solely on phage, incorporating the most recent insights into gene expression in prokaryotes while retaining all the special qualities of the original edition.
Genetic Variation: A Laboratory Manual (pb) Paperback
This manual is the first compendium of protocols specifically geared towards genetic variation studies, and includes thorough discussions on their applications for human and model organism studies. It covers the complete spectrum of genetic variation-from SNPs and microsatellites to more complex DNA alterations, including copy number variation. The early sections of the manual are devoted to study design and generating genotype data, the use of resources such as HapMap and dbSNP, as well as experimental, statistical, and bioinformatic approaches for analyzing the data. The final sections include descriptions of genetic variation in model organisms and discussions of recent insights into human genetic ancestry, forensics, and human variation.
Genome Analysis: Laboratory Manual Series Volume 1: Analyzing DNA
This volume presents exhaustive coverage of techniques for: purifying genomic DNA; manipulating high-molecular weight DNA; several specialized DNA sequencing methods; and computational analysis of DNA and protein sequences. Appendices include recipes for common reagents, basic procedures, safety, and a compilation of ″useful facts.″ Assuming only a basic knowledge of molecular biology, these manuals explain how to clone, manipulate, analyze, and sequence large segments of DNA, and relate sequence to phenotype variation. Techniques, written for human as well as other animal genomes, deal plainly with sources of failure and how to avoid them. Protocols are written by experts, often by those who created the methods. The series is an excellent complement to the bible of recombinant DNA, Molecular Cloning.
Genome Analysis: Laboratory Manual Series Volume 2: Detecting Genes
Topics include Strategies for gene discovery in mammalian systems; Constructing and screening normalized cDNA libraries; Direct cDNA selection; Exon trapping; and Detection of DNA variation Assuming only a basic knowledge of molecular biology, these manuals explain how to clone, manipulate, analyze, and sequence large segments of DNA, and relate sequence to phenotype variation. Techniques, written for human as well as other animal genomes, deal plainly with sources of failure and how to avoid them. Protocols are written by experts, often by those who created the methods. The series is an excellent complement to the bible of recombinant DNA, Molecular Cloning.
Genome Analysis: Laboratory Manual Series Volume 3: Cloning Systems
Assuming only a basic knowledge of molecular biology, these manuals explain how to clone, manipulate, analyze, and sequence large segments of DNA, and relate sequence to phenotype variation. Techniques, written for human as well as other animal genomes, deal plainly with sources of failure and how to avoid them. Protocols are written by experts, often by those who created the methods. The series is an excellent complement to the bible of recombinant DNA, Molecular Cloning. Topics include Bacterial Cloning Systems; Cosmids; Cloning into Bacteriophage P1 Vectors; OBacterial Artificial Chromosomes; and Yeast Artificial Chromosomes
Genome Analysis: Laboratory Manual Series Volume 4, Mapping Genomes
Topics include Meiotic Mapping in Humans; Genetic and Comparative Mapping in Mice; Identification and Analysis of DNA Polymorphisms; DNA Markers for Physical Mapping; Representational Difference Analysis; Somatic Cell Genetics and Radiation Hybrid Mapping; Fluorescence In Situ Hybridization Assuming only a basic knowledge of molecular biology, these manuals explain how to clone, manipulate, analyze, and sequence large segments of DNA, and relate sequence to phenotype variation. Techniques, written for human as well as other animal genomes, deal plainly with sources of failure and how to avoid them. Protocols are written by experts, often by those who created the methods. The series is an excellent complement to the bible of recombinant DNA, Molecular Cloning.
The Human Genome Project was completed in 2003, 50 years after the discovery of the structure of DNA and 17 years after an influential debate at the annual Cold Spring Harbor Laboratory Symposium about the Project's feasibility. The 2003 Symposium was dedicated to examining what has been learned so far from the human genome sequence. This book contains over sixty contributions from the world's leaders in this field and covers genome structure and evolution, methods of data analysis, lessons from species comparison, and the application of sequence data to the understanding of disease.This volume is also published online (annual subscription required) at http://www.cshl-symposium.org. The site contains the full text of the written communications from the 2003 Symposium and the Symposia held in 1998 through 2002 (Volumes LXIII).
The Human Genome Project was completed in 2003, 50 years after the discovery of the structure of DNA and 17 years after an influential debate at the annual Cold Spring Harbor Laboratory Symposium about the Project's feasibility. The 2003 Symposium was dedicated to examining what has been learned so far from the human genome sequence. This book contains over sixty contributions from the world's leaders in this field and covers genome structure and evolution, methods of data analysis, lessons from species comparison, and the application of sequence data to the understanding of disease.This volume is also published online (annual subscription required) at http://www.cshl-symposium.org. The site contains the full text of the written communications from the 2003 Symposium and the Symposia held in 1998 through 2002 (Volumes LXIII).
George Beadle, An Uncommon Farmer: The Emergence of Genetics in the 20th Century
George Beadle was a towering scientific figure whose work from the 1930s to 1960 marked the transition from classical genetics to the molecular era. Among other distinctions, he made the pivotal, Nobel Prize–winning discovery with Edward Tatum that the role of genes is to specify proteins. From 1946 to 1960 he led the Caltech Biology Division, rebuilding it to a powerhouse in molecular biology, and afterwards became a successful President of the University of Chicago. This is the first biography of a giant of genetics, written by two of the field's most distinguished contributors, Paul Berg and Maxine Singer.
In the small Fly Room at Columbia University, T.H. Morgan and his students, A.H. Sturtevant, C.B. Bridges, and H.J. Muller, carried out the work that laid the foundations of modern, chromosomal genetics. The excitement of those times, when the whole field of genetics was being created, is captured in this book, written in 1965 by one of those present at the beginning. His account is one of the few authoritative, analytic works on the early history of genetics. This attractive reprint is accompanied by a website offering full-text versions of the key papers discussed in the book, including the world’s first genetic map.
Illuminating Life: Selected Papers from Cold Spring Harbor, Volume 1 (1903–1969)
The Laboratory that bears the Cold Spring Harbor name is famous for its research, its role in science education, and the science that won three recent Nobel prizes. This book, an intellectual history of Cold Spring Harbor Laboratory, can be enjoyed by anyone interested in the development of contemporary science, regardless of technical background. It is assembled on the foundation of twenty research papers published between 1903 and 1969. Now hard to track down, they are reproduced in facsimile and accompanied by a series of essays by Jan Witkowski describing the investigations that were done, who did them, and why they were important. This handsome volume will appeal to students, established scientists, and others intrigued not only by the history of research at Cold Spring Harbor but also by an extraordinary period in the flowering of American science.
Illustrated Chinese-English Guide for Biomedical Scientists
The Illustrated Chinese-English Guide for Biomedical Scientists is intended to build confidence in the use of English scientific language. The book lists terms that are in common use in science laboratories, translated into both simplified and complex Chinese. It also contains illustrations of equipment, labeled in both languages.
Illustrated Chinese-English Guide for Biomedical Scientists
Many visiting scientists from China, Taiwan and Hong Kong lack confidence in their abilities with the English language. This handbook is aimed to help Chinese-speaking scientists learn English scientific terms. The book is divided into three parts: a cultural guide for working in western laboratories; a list of common terms used in the lab (with translations into both simple and complex Chinese) and illustrations of equipment in common use.
Imaging in Neuroscience and Development: A Laboratory Manual
As imaging studies have continued to expand in scope and sophistication, this edition has expanded to encompass applications in developmental biology. It has more than 20 new chapters on such topics as in vivo imaging in a variety of model systems, calcium imaging, imaging early developmental events, and labeling single neurons. Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes an additional forty shorter chapters of protocols for imaging techniques.
Imaging in Neuroscience and Development: A Laboratory Manual
As imaging studies have continued to expand in scope and sophistication, this new edition of the highly successful and well-received Imaging Neurons: A Laboratory Manual has expanded to include Development, with over 20 new chapters on such topics as MRI microscopy, imaging early developmental events, and labeling single neurons. Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes over forty sections of protocols for imaging techniques.
Immunohistochemistry remains a cornerstone technique in biomedical research, with an ever expanding range of applications. Immunohistochemistry: Methods Express is structured to reflect the modern applications of immunohistochemistry, combining emphasis on the growing trend towards automation and high throughput technology, with clear descriptions of the traditional methodology. The book covers the key techniques, such as: Immunolabeling - specimen preparation, fixation, antigen retrieval, and staining methods; Choice of antibodies and selection of reporter probes; Confocal microscopy and immunohistochemistry; Multiple immunolabeling; Image capture, analysis and quantification; Automation; Quality assurance and troubleshooting
A collection of reprinted articles from the review journal Trends in Biochemical Sciences (TiBS) focusing on the central dogma of molecular biology - DNA makes RNA makes protein. The biographical and autobiographical articles describe the great discoveries in the field from an insider's perspective.
For James D. Watson, the year 2003 was momentous: The 50th anniversary of the discovery, with Francis Crick, of the DNA double helix; the 35th anniversary of the publication of his best–selling memoir of the discovery, The Double Helix; the 35th anniversary of his appointment as Director of Cold Spring Harbor Laboratory, an institution he molded into a research and education center of international renown and prestige: and the year in which the sequencing of the human genome was completed, a project of unprecedented international effort and coordination that Watson got off the ground and sustained during its first, critical years. In the course of his 75 years, Watson has achieved a reputation as outspoken, capricious, abrasive, and ruthless in pursuing his visionary goals. Few other scientists have achieved his celebrity status, or enjoyed it so much, without losing professional credibility. Yet behind the public notoriety there is a complexity apparent only to those who know Watson as a colleague, mentor, inspiration, and friend. This book gives voice to 43 of these individuals—people of distinction who have worked with Watson as a scientist, educator, author, administrator, and government official. Their essays cover much of his scientific life and, taken together, create a portrait of a complex man whose originality and force of will have produced extraordinary achievements.
This book presents the principles of neurobiology from an evolutionary perspective-from single-celled organisms to complex invertebrates such as flies-and is ideal for use as a supplemental textbook. Greenspan describes the mechanisms that allow behavior to become ever more sophisticated-from simple avoidance behavior of Paramecium through to the complex cognitive behaviors of the honeybee-and shows how these mechanisms produce the increasing neural complexity found in these organisms. The book ends with a discussion of what is universal about nervous systems and what may be required, neurobiologically, to be human.
Is It in Your Genes The Influence of Genes on Common Disorders and Diseases that Affect You and Your Family
My father has Parkinson’s Disease. What is my risk? My older sister has schizophrenia. I am 45 and healthy. What are the risks to my kids? My 37 year old sister has just been diagnosed with multiple sclerosis. Am I at increased risk?
In this book, Dr Philip Reilly, a well-known author and clinical geneticist, attempts to answer these questions and many more. Our increased understanding of the influence of genes on disease means the nature of genetic risks in families is becoming ever clearer. The book covers over 90 common conditions, diseases and disorders ranging in chronological order from conception to old age. Dr Reilly discusses each one of these in turn, making clear what is known and not known about the genetic factors influencing the development of the disease. He also offers advice on what you, or other members of your family, can do if you are at risk of developing a disease. Written in clear, non-technical terms, this book is a vital read for anyone trying to understand the causes and effects of diseases running through their family.
Is It in Your Genes The Influence of Genes on Common Disorders and Diseases that Affect You and Your Family
My father has Parkinson’s Disease. What is my risk? My older sister has schizophrenia. I am 45 and healthy. What are the risks to my kids? My 37 year old sister has just been diagnosed with multiple sclerosis. Am I at increased risk?
In this book, Dr Philip Reilly, a well-known author and clinical geneticist, attempts to answer these questions and many more. Our increased understanding of the influence of genes on disease means the nature of genetic risks in families is becoming ever clearer. The book covers over 90 common conditions, diseases and disorders ranging in chronological order from conception to old age. Dr Reilly discusses each one of these in turn, making clear what is known and not known about the genetic factors influencing the development of the disease. He also offers advice on what you, or other members of your family, can do if you are at risk of developing a disease. Written in clear, non-technical terms, this book is a vital read for anyone trying to understand the causes and effects of diseases running through their family.
Scientists are trained in scholarship and technical skills but not, typically, in how to deal with their peers, supervisors, or staff who report to them. Yet even a first-rate research project can fail or flounder if the people concerned can't get along. Lab Dynamics is a book about the challenges of doing science and dealing with the individuals involved, including oneself. The authors, a scientist and a psychotherapist, draw on principles of group and behavioral psychology but speak to scientists in their own language. They offer in-depth, practical advice, real-life examples, and exercises tailored to scientific and technical workplaces on topics as diverse as conflict resolution, negotiation, dealing with supervision, working with competing peers, and making transitions between academia and industry.
Lab Math: A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench
Work at the biology bench requires a knowledge of mathematical methods and formulae. This book is a compilation of the mathematical concepts and methods in molecular biology, and provides guidance on their application to research investigations. Subjects range from basics such as scientific notation and measuring and making solutions, to more complex activities like quantifying and designing nucleic acids and analyzing protein activity. A reference section features useful tables, conversion charts and "plug and chug" equations for experimental procedures. This volume is a structured source of information that in many laboratories is scattered.
Lab Ref, Volume 2: A Handbook of Recipes, Reagents, and other Reference Tools for Use at the Bench
This book is a handy benchtop source of recipes and information needed for common laboratory protocols. Volume 2 contains recipes for procedures as diverse as RNA interference, imaging, proteomics, and quantitative nucleic acid analysis, and is organized by application for quick and easy reference. All recipes were derived from recent manuals published by Cold Spring Harbor Laboratory Press, and each recipe is cross-referenced to its manual of origin. Reference tables and charts are included. To meet the growing need for computational resources in the biology laboratory, this book also has an expanded Web site section with sources for biological materials, databases for acquiring and annotating genomic and proteomic data, and links to downloadable software.
Lab Ref: A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench
This handbook of valuable information extracted from laboratory manuals published by Cold Spring Harbor Laboratory Press is presented in an easy-to-use format. It contains invaluable reference data, never before assembled in one handy package. It has been assembled from extensively field-tested manuals, ensuring accuracy and reliability, by two scientists with extensive and diverse experience of laboratory practice.
Landmark Papers in Cell Biology: Selected Research Articles Celebrating Forty Years of The American Society for Cell Biology
Spectacular advances are being made in our understanding of cells, from the beautiful intricacy of their structure to the incredible complexity of the biochemical reactions and pathways that regulate their behavior. Published to mark the 40th anniversary of The American Society for Cell Biology, Landmark Papers in Cell Biology contains reprints of 42 seminal papers that illustrate the field’s great advances along with brief commentaries that place the papers in historical and thematic context. Highlighting advances in cell biology since the founding of the Society in 1960, the papers - all studies of eukaryotes - were carefully screened and chosen for their significance in the field by eminent cell biologists Joseph Gall and Richard McIntosh. The articles in this timely reference are grouped according to the following themes: Genome organization and replication Transcription Nuclear envelope and nuclear import Mitosis and cell cycle control Cell membrane and extracellular matrix Protein synthesis and membrane traffic Cytoskeleton
The yeasts have been important experimental organisms for more than 50 years. This volume contains introductions to over 60 selected papers, in sections that describe the process of discovery and the context and significance of the research described in the papers. The papers referenced include early classics as well as recent advances in areas such as signal transduction, membrane trafficking, protein turnover, and genomics. This book is designed as a guide for a literature–based course. The Landmarks Web Site: Accompanying the book, the Landmarks Web site lists the selected papers, along with links to their abstracts in PubMed and to the journals' home pages, where the full text will be available if the readers or their institutions have appropriate subscriptions or if the journals have released their back issues free. The Landmarks Web site is free to access but will require registration. THIS BOOK WILL PUBLISH IN THE FUTURE. SIGMA-ALDRICH CAN ACCEPT ORDERS NOW FOR FULFILLMENT WHEN PUBLISHED.
This book is about mobile genes - the transfer of DNA between unrelated cells. It discusses the machinery of gene transfer and its wide-ranging biological and health consequences. Mobile DNA makes possible the development of antibiotic resistance in microbes, the conversion of harmless to pathogenic bacteria, and the triggering of cancerous growth in cells. It also contributes to human evolution.
This book is about mobile genes - the transfer of DNA between unrelated cells. It discusses the machinery of gene transfer and its wide-ranging biological and health consequences. Mobile DNA makes possible the development of antibiotic resistance in microbes, the conversion of harmless to pathogenic bacteria, and the triggering of cancerous growth in cells. It also contributes to human evolution. This well-illustrated volume contains an up-to-date account of a topic now seen as increasingly important, and will be invaluable for both working scientists and as a textbook for advanced courses.
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a CD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book.
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a CD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book.The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Molecular techniques have provided remarkable insights into the natural history of lymphoid tumors and now suggest novel experimental approaches to their treatment. This volume, designed for both the physician and the clinical scientist, reviews the pathogenesis and diagnosis of the major types of lymphoma and concludes with accounts of promising therapeutic approaches involving gene therapy, antisense nucleic acid, and targeted, specific antibodies.
Manipulating the Early Embryo of Xenopus laevis: A Video Guide
The large, readily available eggs of enopus laevis are a valuable subject for study. This 3-volume video set contains 68 demonstrations of the manipulation of living eggs using techniques taught in the course on the Early Development of Xenopus laevis held at Cold Spring Harbor Laboratory, New York, for the past four years. The video was produced using digital techniques to ensure no loss of resolution of the fine images captured by microphotography. Recorded live among the course participants, the videotape accurately reflects the triumphs and tribulations of real investigators as they attempt painstaking procedures while commenting on their actions for the students’ benefit. This video guide is an essential learning tool for investigators considering work with Xenopus eggs and embryos.
Manipulating the Mouse Embryo: A Laboratory Manual (Third Edition)
Regarded as the "Bible" of mammalian embryo manipulation techniques since the 1986 publication of the first edition of Manipulating the Mouse Embryo: A Laboratory Manual, the third edition of this essential laboratory resource has now been completely reorganized, rewritten, and updated by a new cast of authors. The result is a compilation of new, cutting–edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques. The "gold standard" techniques for applying recombinant DNA technology to investigations of mammalian embryonic development included in the first two editions of this book are also updated and recast, as is the summary of the current state of understanding of mouse development at the molecular level. This book is the premier authoritative and comprehensive source of technical and theoretical guidance for mouse developmental biologists and geneticists and is an essential resource for newcomers to these fields. Key features of the third edition: New authors are leading authorities in mouse development and genetics, as were authors of prior editions. All new illustration and new design: All illustrations have been redrawn; extensive use of new illustrations and photographs of techniques and results; a new, contemporary design presents techniques more clearly. New explanatory text: More background information for techniques is presented as well as more extensive troubleshooting information. New, functional organization and techniques.
Manipulating the Mouse Embryo: A Laboratory Manual, 3rd ed.
Regarded as the "Bible" of mammalian embryo manipulation techniques, the third edition has been completely reorganized and rewritten. The result is a compilation of new, cutting-edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques.
Complex macromolecular machinery is responsible for gene-specific and cell-type selective patterns of gene expression. How it works is being revealed by increasingly sophisticated molecular dissection of prokaryotic and eukaryotic cells. In this volume, sixty-seven of the world’s leaders in the study of gene transcription review the results and conclusions of their most recent studies. They cover events ranging from activation, through promoter recognition, repression, chromosome structure, chromatin remodeling, initiation and elongation, and the regulation of stopping and starting. Written with a broad audience of molecular biologists in mind, extensively illustrated and completed by a masterly overview from Richard Losick, this book is an up-to-date and accessible survey of a topic at the heart of molecular biology and biotechnology.
This latest book by Elof Carlson (The Unfit)is a first history of classical genetics, the era in which the chromosome theory of heredity was proposed and developed. Highly illustrated and based heavily on early 20th century original sources, the book traces the roots of genetics in breeding analysis and studies of cytology, evolution, and reproductive biology that began in Europe but were synthesized in the United States through new Ph.D. programs and expanded academic funding. Carlson argues that, influenced largely by new technologies and instrumentation, the life sciences progressed though incremental change rather than paradigm shifts, and he describes how molecular biology emerged from the key ideas and model systems of classical genetics. Readable and original, this narrative will interest historians and science educators as well as today's practitioners of genetics.
Yeast is an ideal eukaryotic microorganism for biochemical and genetic studies, particularly since its complete genome sequence became available. This Cold Spring Harbor Laboratory Yeast Genetics Course Manual combines essential standard methods with the latest technologies. In this edition, most of the experiments have been revised and several new experiments and techniques have been added, including the 2-hybrid system, PCR-based methods, and isolation of chromatin.
This is an updated edition which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents used in the study of yeast biology. Since the last edition of the manual was published (2000), revolutionary advances in genomics and proteomics technologies have had a significant impact on the field. This updated edition reflects these advances, and also includes new techniques involving vital staining, visualization of Green Fluorescent Protein, new drug resistance markers, high-copy suppression, Tandem Affinity Protein tag protein purification, gene disruption by double-fusion polymerase chain reaction, and many other recent developments.
This edition of Watson's classic textbook has been brought up to date, and incorporates insights very recently derived from genome sequencing in a variety of organisms. The book is a comprehensive survey of the fundamentals of molecular biology, from basic mechanisms to the elaborate role of gene regulation in embryonic development and evolution. The 21 chapters retain the distinctive features of the original work, including introductory chapters on the history of genetics and molecular biology and an emphasis throughout on the chemical underpinnings of molecular biology. By revealing the intellectual framework and experimental approaches that made new discoveries in the field possible, this edition highlights the significance of the molecular approach for all of biology.
Molecular Cloning: A Laboratory Manual, 3rd ed., Vols 1,2 and 3
In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved. It includes 240 laboratory protocols in DNA science in which over 35% were created especially for this edition, along with coverage of bioinformatics and DNA microarrays.
The gene has become a cultural icon and an increasingly rich source of imagery and ideas for visual artists. Drawing on a wide range of contemporary painting and sculpture, The Molecular Gaze: Art in the Genetic Age explores the moral and bioethical questions these works address. What does it mean to be human? What is identity in a society of genetically manipulated individuals? Questions like these are growing louder as genetic technology advances and the public examines the ethical consequences more widely. Suzanne Anker and Dorothy Nelkin, an artist and a social scientist, have written a thought-provoking and visually fascinating book for scientists, artists, students, and general readers intrigued by the anxiety and exhilaration of the genetic age.
The generation of mutant mice raises many questions about the best means of phenotypic analysis, breeding, and maintenance. The answers are now available from two experts with a wealth of detailed knowledge never previously assembled in one volume. Informal and highly practical, this handbook provides step-by-step methods for troubleshooting experiments, from the basics of gene targeting through the analysis of postnatal effects. All investigators using the laboratory mouse in genetic studies designed to understand mammalian biology will benefit from the authors' experience and advice.
Murderous Science: Elimination by Scientific Selection of Jews, Gypsies, and Others in Germany 1933-1945
The Human Genome Project has associated many mutant genes with physical ailments and the genetic basis of certain behavioral characteristics is being seriously discussed. In the 1920s and 1930s, advocates for eugenics claimed that genes influenced human behavior, but with no valid evidence. In Germany the Nazis adopted their ideas to justify violent anti-semitism. In this new, expanded edition of the English translation of his compelling book.
This unique work of reference provides quick and easy access to the remarkable diversity of mutant phenotypes expressed by the maize plant. Its major portion consists of over 400 color photographs of well-established mutants arranged by chromosome position, detailed descriptions of the mutants' gene loci, and a current summary of the physical structure of genes that have been characterized at the molecular level. First published in 1968 and long out of print, Mutants of Maize has been entirely revised by three of the corn plant's most distinguished investigators, enlarged to cover twice the number of mutants, and redesigned with clarity and elegance. All maize biologists will want this magnificent book in their personal library. In addition, because mutant maize genes have counterparts in most species, the book can be considered a general guide to the consequences of mutation in the entire plant kingdom. All maize biologists will want this magnificent book in their personal library. In addition, because mutant maize genes have counterparts in most species, the book can be considered a general guide to the consequences of mutation in the entire plant kingdom.
This unique work of reference provides quick and easy access to the remarkable diversity of mutant phenotypes expressed by the maize plant. Its major portion consists of over 400 color photographs of well-established mutants arranged by chromosome position, detailed descriptions of the mutants' gene loci, and a current summary of the physical structure of genes that have been characterized at the molecular level. First published in 1968 and long out of print, Mutants of Maize has been entirely revised by three of the corn plant's most distinguished investigators, enlarged to cover twice the number of mutants, and redesigned with clarity and elegance. All maize biologists will want this magnificent book in their personal library. In addition, because mutant maize genes have counterparts in most species, the book can be considered a general guide to the consequences of mutation in the entire plant kingdom. All maize biologists will want this magnificent book in their personal library. In addition, because mutant maize genes have counterparts in most species, the book can be considered a general guide to the consequences of mutation in the entire plant kingdom.
This second edition of a much praised and widely used manual has been entirely revised and updated. Each technique is presented with extensive background information, advice, and troubleshooting. All current applications of PCR are covered in protocols that have the hallmark reliability of the previous edition.
This second edition of a much - praised and widely used manual has been entirely revised and updated. Each technique is presented with extensive background information, advice, and troubleshooting. All current applications of PCR are covered, in protocols that have the hallmark reliability of the previous edition.
A principal architect and visionary of the new biology, a Nobel Prize-winner at 34 and best-selling author at 40 (The Double Helix), James D. Watson had the authority and courage to take a prominent role as commentator on the march of DNA science and its implications for society. In essays for publications and in lectures, he delivered what were, in effect, dispatches from the front lines of the revolution. This book is a collection of outspoken and sparkling ideas and opinions.
Phage display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage display technology is challenging and the aim of this manual is to provide comprehensive instruction in its theory and application.
This volume is a new edition of the most authoritative book on Prion Biology, first published in 1999 and edited by the Nobel Prize–winning founder of the field. This expanded edition has been completely updated, and includes chapters on therapeutics, and diagnostic methods and approaches This book is by far the most authoritative summary of the complex data that form the basis of our understanding of the molecular basis of this esoteric group of diseases.
Protein-Protein Interactions: A Molecular Cloning Manual
As more genetic and biochemical information about the protein components of cells accumulates, the analysis of protein-protein interactions is becoming increasingly important. This manual presents a wide range of techniques for identifying and analyzing these interactions, starting with standard molecular and biochemical techniques, and progressing to biophysical and computational approaches and therapeutic and other post-genomic applications. This manual is a complement to the best selling laboratory manual "Molecular Cloning".
Protein-Protein Interactions: A Molecular Cloning Manual, 2nd Edition hard cover
This is an updated edition of a manual that provides a thorough collection of the technical and theoretical issues involved in the study of protein associations, including standard methods, biophysical approaches, and, in a final section, a collection of computational methods for integrating and analyzing interactions.
Protein-Protein Interactions: A Molecular Cloning Manual, 2nd Edition soft cover
This is an updated edition of a manual that provides a thorough collection of the technical and theoretical issues involved in the study of protein associations, including standard methods, biophysical approaches, and, in a final section, a collection of computational methods for integrating and analyzing interactions.
Completion of sequencing of the human genome has opened the door for exploration of the proteome. Critical to tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying, isolating, and quantifying proteins in cells and cell pathways and for performing functional assays. This book provides the first authoritative and protocol-based approach to proteomics. It provides information about protein structure and numerous methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics. Extensive background information and references are provided regarding the theoretic aspects of the techniques and their applications.
Completion of sequencing of the human genome, as well as those of many other organisms, has now opened the door for exploration of the proteome, the many thousands of interacting proteins in a given organism. Critical to tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying, isolating, and quantifying proteins in cells and cell pathways and for performing functional assays. Proteins and Proteomics: A Laboratory Manual, authored by Richard Simpson of the Ludwig Institute for Cancer Research, Melbourne, provides the first authoritative and wide–ranging protocol-based approach to proteomics. Presented as a logical strategy for analyzing proteomes, Proteins and Proteomics provides information about protein structure and numerous methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics. Extensive background information and references are provided regarding the theoretic aspects of the techniques presented as well as their applications. This manual is an essential laboratory tool for genetics and molecular biology investigators moving from studies of genomics and genotype to those of proteomics and phenotype. It is invaluable for cell biologists, developmental biologists, and neuroscientists exploring the intricacies of the proteome. It will also be an excellent resource for protein chemists and biochemists.
Purifying Proteins for Proteomics: A Laboratory Manual
This manual complements Simpson’s Proteins and Proteomics manual, with a comprehensive collection of methods for protein purification from a variety of source preparations. The chapters include detailed protocols, methods for optimizing the performance of experiments, discussion of potential pitfalls, and troubleshooting advice.
The striking functional versatility of RNA is attributable to its remarkable capacity for conformational change. Current models of RNA synthesis, its maturation, translation, and degradation are all based on specific structures and there is growing appreciation of the functional importance of interactions between RNA and RNA-binding proteins. This book identifies the major intellectual and technical advances in understanding the part played by structure in how RNA works. It encompasses the experimental approaches used to define RNA structure and its influence on RNA functions such as transcription termination, catalysis, recoding, and translational control. Investigators with these interests and students of molecular biology will find this volume a thoroughly up-to-date, provocative survey of a rapidly advancing field.
Recent studies on the activities of RNA in the cell have revolutionized our understanding of the many roles played by this molecule. The first two editions of The RNA World (1993, 1999) shed light on the pre–biotic era dominated by this versatile molecule, and provided an overview of the state of RNA research at the time. The new third edition updates this perspective, describing the vast array of newly discovered roles for RNA in the modern world. The updated original chapters are supplemented with new chapters on RNA–protein complexes, snRNPs and snoRNPs, telomerase RNA, RNAi, microRNAs, noncoding RNA, and many other subjects. This book is essential reading on the biology of nucleic acids and gene regulation.
The use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific, remarkably potent, and acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and cheaper way of mimicking the effects of mutations in both cell cultures and in living animals. This book presents the principles of RNA interference and reliable protocols for its laboratory use in C. elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more.
The use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific, remarkably potent, and acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and cheaper way of mimicking the effects of mutations in both cell cultures and in living animals. This book presents the principles of RNA interference and reliable protocols for its laboratory use in C. elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more.
For over 25 years the study of retroviruses has underpinned much of what is known about information transfer in cells and the genetic and biochemical mechanisms that underlie cell growth and cancer induction. Emergent diseases such as AIDS and adult T-cell lymphoma have widened even further the community of investigators directly concerned with retroviruses, a development that has highlighted the need for an integrated understanding of their biology and their unique association with host genomes. This remarkable volume satisfies that need. Written by a group of the field's most distinguished investigators, rigorously edited to provide a seamless narrative, and elegantly designed for clarity and readability, this book is an instant classic that demands attention from scientists and physicians studying retroviruses and the disorders in which they play a role.
For over 25 years the study of retroviruses has underpinned much of what is known about information transfer in cells and the genetic and biochemical mechanisms that underlie cell growth and cancer induction. Emergent diseases such as AIDS and adult T-cell lymphoma have widened even further the community of investigators directly concerned with retroviruses, a development that has highlighted the need for an integrated understanding of their biology and their unique association with host genomes. This remarkable volume satisfies that need. Written by a group of the field's most distinguished investigators, rigorously edited to provide a seamless narrative, and elegantly designed for clarity and readability, this book is an instant classic that demands attention from scientists and physicians studying retroviruses and the disorders in which they play a role.
This is a slim, benchtop reference collection of tips and warnings on the safe handling of chemicals and biologicals, listed alphabetically by compound. Extracted from a wide range of Cold Spring Harbor Laboratory Press manuals, this valuable information is presented in a handy format designed for easy use. The volume also includes helpful charts and tables.
For the past two decades, much effort has been made to understand the complex interactions between lymphocytes and antigen-presenting cells that result in immune responses. Immunologists have identified an extraordinary diversity of cytokines as players in these interactions, some the products of cellular activation which themselves activate surrounding cells. Meanwhile, another community of investigators, interested in how genes turn on, were using lymphocytes as easily cultivated cells with which to study transcriptional regulation from a molecular perspective. Connections between these areas of study, with their quite different technical vocabularies and experimental approaches, were limited. The 64th Annual Cold Spring Harbor Symposium was therefore a particularly imaginative meeting, intended to provoke a dialogue on the nature of the membrane signals and intracytoplasmic events that provoke the generation of immunity. It did. The debate ranged over biochemistry, physiology, and molecular genetics as well as classical cellular immunology, involving over 70 of the world's leading investigators in a discussion described by the summarizer as the best he had ever attended. The book prompted by the meeting contains personal summaries of the contributions these investigators have made to their fields over the years, with reference to their most recently reported data. This volume therefore contains a remarkably fresh and original perspective on an important aspect of immunology that is necessary to understand in order to undertake rational manipulation of immune responses. All laboratories committed to the future of immunology should have this book, as well as investigators in gene regulation seeking a wide-ranging survey of results achieved with lymphocytes.
For the past two decades, much effort has been made to understand the complex interactions between lymphocytes and antigen-presenting cells that result in immune responses. Immunologists have identified an extraordinary diversity of cytokines as players in these interactions, some the products of cellular activation which themselves activate surrounding cells. Meanwhile, another community of investigators, interested in how genes turn on, were using lymphocytes as easily cultivated cells with which to study transcriptional regulation from a molecular perspective. Connections between these areas of study, with their quite different technical vocabularies and experimental approaches, were limited. The 64th Annual Cold Spring Harbor Symposium was therefore a particularly imaginative meeting, intended to provoke a dialogue on the nature of the membrane signals and intracytoplasmic events that provoke the generation of immunity. It did. The debate ranged over biochemistry, physiology, and molecular genetics as well as classical cellular immunology, involving over 70 of the world's leading investigators in a discussion described by the summarizer as the best he had ever attended. The book prompted by the meeting contains personal summaries of the contributions these investigators have made to their fields over the years, with reference to their most recently reported data. This volume therefore contains a remarkably fresh and original perspective on an important aspect of immunology that is necessary to understand in order to undertake rational manipulation of immune responses. All laboratories committed to the future of immunology should have this book, as well as investigators in gene regulation seeking a wide-ranging survey of results achieved with lymphocytes.
The 1949 publication The Structure and Reproduction of Corn, by Theodore Kiesselbach is an indispensable source of knowledge for plant biologists throughout the world but has long been unavailable in printed form. To mark the fiftieth anniversary of the appearance of this important work, a new edition has been published on high quality paper that brings out the best in the text’s finely detailed illustrations. This edition includes a historical introduction that illuminates the man behind the science, an investigator who applied the lessons of studies of hybrid vigor to create a revolution in corn breeding that transformed the economy of the American prairie states. This small, attractive book is a must for everyone interested in plant development.
A comprehensive account of telomere structure and function in a variety of organisms including yeast, Drosophila, ciliates, plants and mammals is presented. Topics covered include: an historical overview, telomere dynamics, localization, transcriptional silencing and the significance of length regulation.
An up-to-date survey of the current exciting state of telomere biology. Telomeres, specialized structures found at the ends of chromosomes, are essential for maintaining the integrity of chromosomes and their faithful duplication during cell division. Chapters in this volume cover telomere structure and function in a range of organisms, focusing on how they are maintained, their roles in cell division and gene expression, and how deficiencies in these structures contribute to cancers and other diseases and even aging.
Dogs of different breeds can range remarkably in size, shape, and behavior, and yet they all carry essentially the same genome, making them a particularly fascinating model for genome plasticity. The recent release of the complete sequence of the dog genome provides an exciting new context in which to consider such variation. Twenty-five chapters written by experts in the field include various aspects of morphological and behavioral variation in dogs, their origins and domestication, and their unique value as a model system for many common but complex human diseases such as diabetes and cancer.
This is the first book to contain the newly published findings on the structure of the ribosome and discuss their meaning for our understanding of how proteins are made and processed inside the cell. With over 60 contributions from the world’s most innovative ribosome biology laboratories, this is the latest volume in the annual series that for over 60 years has provided analysis and interpretation of the most interesting problems in biology.
This is the first book to contain the newly published findings on the structure of the ribosome and discuss their meaning for our understanding of how proteins are made and processed inside the cell. With over 60 contributions from the world’s most innovative ribosome biology laboratories, this is the latest volume in the annual series that for over 60 years has provided analysis and interpretation of the most interesting problems in biology.
In a new preface to this special edition of his critically acclaimed memoir, Francois Jacob recalls the events that brought him to Cold Spring Harbor Laboratory in the early 1960's and taught him much about phage biology and the informal ways of American science. Throughout his book, Jacob demonstrates a scientist's eye for detail and a poet's instinct for the inner life, as he tells of a privileged Parisian boyhood, young love, heroism in war, and the fascination of life at the edge of scientific discovery.
The Unfit, by Elof Carlson, explores the sources of a movemen - negative eugenics - that was used to justify the Holocaust, which claimed millions of innocent lives in World War II. The title reflects the nearly three centuries of belief that some people are socially unfit by virtue of a defective biology, and echoes an earlier theory of degeneracy, dating to biblical antiquity, in which some people were deemed unfit because of some transgression against religious law. The author presents the first biological theory of degeneracy - onanism - and then follows the development of degeneracy theory throughout the nineteenth century and its application to a variety of social classes. The key intellectual theories and their proponents form the framework of this exploration, which includes the concepts of evolution and heredity and how they were applied to social problems. These ideas are followed into the twentieth century with the development of theories of positive and negative eugenics, the establishment of compulsory sterilization laws, racism and anti-Semitism, and the Holocaust. This story of misapplied science and technology is one that still haunts humanity in the twenty-first century. The ghost of eugenics recurs in many guises during debates and controversies about intelligence testing, genetic screening, prenatal diagnosis, gene therapy, new reproductive strategies, and uses of our genomic information. Carlson ends his discussion of the history of humanity in this arena with an exploration of the future of genetics that is based on new technologies and application of the Human Genome Project findings, as well as a discussion of the death of the old eugenics and of the problems that will not go away, including our ambivalence about our own biology.
Times of Triumph, Times of Doubt: Science and the Battle for Public Trust
The intent and uses of science are a continuing preoccupation, especially in public debates on issues such as new pharmaceuticals, cloning, stem cells, genetically modified foods, and assisted reproduction. Times of Triumph, Times of Doubt, written by the eminent geneticist and historian Elof Carlson, explores the moral foundations of science and their role in these hot–button issues. Carlson chooses a variety of case histories and describes their scientific background and the part played by scientists in the application of their work, including their motivations and reactions to bad outcomes, both real and alleged. He examines why ethical lapses have occurred in these areas, why bad things happen when, for the most part, those who worked on the science had only good intentions in mind, and how such lapses can be prevented from occurring in the future.
Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniques
In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Transcriptional Regulation in Eukaryotes answers that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene and the biochemistry of a new transcription factor.
The new edition of this successful monograph has been both updated and broadened. Since the previous (second) edition was published in 2000, the structures of the bacterial and eukaryotic ribosomes have been published, advancing our basic understanding of translation and mechanisms involving protein and RNA regulators. In addition, as the title indicates, this edition has a new focus on the role of translational control in human development and disease. This book, with 30 chapters written by experts in the field, is essential reading for anyone interested in the process of translation, its regulation, and how its failure can be the cause of disease.
Since the 1996 publication of Translational Control, there has been fresh interest in protein synthesis and recognition of the key role of translational control mechanisms in regulating gene expression. This new monograph updates and expands the scope of the earlier book but it also takes a fresh look at the field. In a new format, the first eight chapters provide broad overviews, while each of the additional twenty-eight has a focus on a research topic of more specific interest. The result is a thoroughly up-to-date account of initiation, elongation, and termination of translation, control mechanisms in development in response to extracellular stimuli, and the effects on the translational machinery of virus infection and disease.
Harlow and Lane have revised their guide to the use of immunoglobulin reagents in the laboratory. Chapters have been rewritten, reorganized, and updated to provide background, context and step-by-step instructions. Techniques range from choosing the right antibody and handling it correctly to the proper methods for characterizing antigens in cells and solutions. New chapters on tagging proteins and epitope mapping are included. Rather than presenting an array of solutions for working with antibodies and antigens, this title instead identifies in each case the best approach to specific problems.
We Can Sleep Later: Alfred D. Hershey and the Origins of Molecular Biology
An absorbing portrait of the pioneering molecular biologist best known for demonstrating that DNA is the genetic component of phages, through essays and reminiscences from twenty–three distinguished scientists whose work and careers were influenced by the man and his science.
Whole Genome Amplification: Methods Express Series Hardcover
Using Whole Genome Amplification (WGA) methods, it is possible to create microgram quantities of DNA starting with as little as one nanogram of genomic DNA and in some cases even a single eukaryotic or bacterial cell. The implementation of such WGA methods provides an ample supply of DNA for large-scale genetic studies. This title provides a comprehensive overview of the field and will be welcomed by all researchers looking to take advantage of the latest developments.
Whole Genome Amplification: Methods Express Series Softcover
Using Whole Genome Amplification (WGA) methods, it is possible to create microgram quantities of DNA starting with as little as one nanogram of genomic DNA and in some cases even a single eukaryotic or bacterial cell. The implementation of such WGA methods provides an ample supply of DNA for large-scale genetic studies. This title provides a comprehensive overview of the field and will be welcomed by all researchers looking to take advantage of the latest developments.
Writing Life of James D. Watson: Professor, Promoter, Provocateur
James Watson's fame as a scientist and research leader overshadows his considerable achievements as an innovator in the form and style of scientific communication. This book surveys Watson's books and essays from the perennially best-selling The Double Helix through his classic textbooks of the 1960s and 70s, polemics on ethical questions about genetic technology, to more recent works of autobiography.
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