This comprehensive review of our current understanding of adult stem cell types and their regulation examines the derivation, characterization, and utility of adult stem cells found in skeletal muscle, dental pulp, prostate, kidney, the eye, inner ear, and other tissues. The authors pay special attention to adult stem cell plasticity and their utility in normal tissue homeostasis, as well as their applications in regenerative medicine.
This book is a guide to all those areas which are currently basic to the performance of animal (including human) cell culture. Experienced and novice researchers should find this book useful as a source of reference, when entering a new field, or when setting up a new laboratory.
This edition, devoted to those cells that line the body protecting surfaces and secreting or absorbing material, provides revised descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. Each chapter is devoted to a different type of epithelium--epidermal, mammary, cervical, prostatic, oral, intestinal, pulmonary, hepatic, and urothelial. Freshney contributes a final chapter on a number of epithelia not covered in the main chapters, presenting them together in review form. Other features include alternate techniques coupled with critical comparative analyses and new chapters on the culture of oral, alveolar, intestinal, and urothelial epithelium.
This book describes productive methods for using embryonic stem (ES) cells as in vitro developmental models for many cell and tissue types. These detailed protocols range from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries, to the generation of antibodies against very rare antigens, and to the identification of and characterization of protein and protein interactions. This will prove an invaluable resource for those actively using, or planning to use ES cells, to study fate choices and specific lineages.
A team of experimenters and researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. The emphasis is on the analysis and assessment of epithelial cells, for example by looking at apoptosis and integrins, or by measuring membrane capacitance and confluence. Also described in detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria.
This abridged version of the bestselling reference Handbook of Stem Cells, Two-Volume Set attempts to incorporate all the essential subject matter of the original two-volume edition in a single volume. The material has been reworked in an accessible format suitable for students and general readers interested in following the latest advances in stem cells, including full color presentation throughout. Although some extra language and chapters have been deleted, rigorous effort has been made to retain from the original two-volume set the material pertinent to the understanding of this exciting area of biology. The organization of the book remains largely unchanged, combining the prerequisites for a general understanding of adult and embryonic stem cells; the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations; as well as a presentation by the world's experts of what is currently known about each specific organ system.
A discussion of all the key issues in the use of human pluripotent stem cells for treating degenerative diseases or for replacing tissues lost from trauma. On the practical side, the topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials. Regulatory issues are addressed in discussions of the ethical debate surrounding the derivation of human embryonic stem cells and the current policies governing their use in the United States and abroad, including the rules and conditions regulating federal funding and questions of intellectual property.
This text begins by addressing the basic biology of the cells, in the context of the other stem cell populations present in fetuses and adult organisms. Discussion then turns to how stem cells can be used to further the understanding of human developmental processes, which currently lags far behind that of model organisms. Several chapters describe attempts to generate stem cell derived specific lineages as potential tools for cell based therapeutics, and these are followed by discussion of the legal and moral issues which accompany this ground breaking research.
Human Embryonic Stem Cells: An Introduction to the Science and Therapeutic Potential
The social debate involving the creation of human embryos for stem cell therapy prompted the writing of this text. The emerging field of human embryonic stem cell biomedicine crosses many disciplinary boundaries - cell biology, reproductive biology, embryology, molecular biology, endocrinology, immunology, fetal medicine, transplantation medicine and surgery. This single reference provides basic information from these multiple disciplines as it pertains to the science of stem cells.
This edition reflects the increase in our knowledge of the development of the nervous system. With an understanding that the regionalization of stem cells occurs early in development and that this bias appears to persist even after prolonged culture, Dr. Rao has added additional chapters on olfactory epithelial stem cells and retinal stem cells, both of which differ in their properties from ventricular zone and subventricular zone-derived neural stem cells. A new chapter on cell death summarizes the important changes in the death pathway that occurs as stem cells mature. Special attention is paid to the derivation of neural cells from embryonic stem cells. This book provides neuroscientists with a guide to stem cells in the nervous system, tracing with clarity the development of stem cells from differentiation to neurons, astrocytes, and oligodendrocytes.
Recognized experts describe the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their culture, and make it possible to define their properties in culture. The techniques are designed to help researchers identify endogenous stem cells as well as exogenous stem cells after transplantation in the brain. Protocols range from the simplest methods of isolation and characterization of neural cell properties to such sophisticated methods as characterizing gene expression, telomerase assays, and cell cycle kinetics.
Stem Cell Tools and Other Experimental Protocols : Methods in Enzymology Volume 420
This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook” and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional” and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives.
This survey the "state-of-the-science" of stem cell biology explains the origins of stem cells and describes how they function, how they can cause illness, and how they might be employed to cure or ameliorate disease. In particular, the authors discuss the roles of stem cells in development and organogenesis, in normal tissue turnover, in the repair response to injury, and in carcinogenesis. They also explain how to obtain stem cells from different organs, culture them in vitro, and manipulate them for therapeutic use.
Image 
