Bovine Serum Albumins

Bovine serum albumin (BSA) is a globular protein (~66,000 Da) that is used in numerous biochemical applications due to its stability and lack of interference within biological reactions.

Sigma provides a variety of Bovine serum albumins with purities ranging from 95-99%. Sigma-Aldrich manufactures and distributes bovine serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. For convenience our BSA′s are defined below by method of preparation.

Chromatographically Purified

Chromatographic purification of albumin typically yields a more highly conserved native protein conformation than the ethanol and heat shock fractionation methods.

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A2058 Bovine Serum Albumin lyophilized powder, essentially IgG-free, low endotoxin, BioReagent, suitable for cell culture
A1933 Bovine Serum Albumin chromatographically purified, New Zealand origin, low endotoxin, suitable for cell culture, pH 7, ≥98%
A9306 Bovine Serum Albumin lyophilized powder, low endotoxin, ≥95% (agarose gel electrophoresis)
A3675 Bovine Serum Albumin lyophilized powder, low endotoxin, ≥98% (agarose gel electrophoresis)
A4737 Bovine Serum Albumin lyophilized powder, protease, essentially free, ≥98% (agarose gel electrophoresis)
A4612 Bovine Serum Albumin lyophilized powder, Essentially fatty acid free

Ethanol and Heat Shock

A method which combines the ethanol and heat shock processes using Cohn fractionation to perform the initial purification followed by a heat step to further purify the albumin to ≥ 99% by Agarose electrophoresis.

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A0281 Bovine Serum Albumin lyophilized powder, essentially fatty acid free and essentially globulin free, ≥99% (agarose gel electrophoresis)
A7638 Bovine Serum Albumin lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)
A4161 Bovine Serum Albumin lyophilized powder, essentially globulin free, BioReagent, suitable for cell culture
A3156 Bovine Serum Albumin lyophilized powder, γ-irradiated, Globulin Free, BioXtra, suitable for cell culture

Ethanol Fractionated

A method of fractionation which includes the adjustment of ethanol concentration, pH, and temperature. Serum albumin may be referred to as Cohn Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Unlike the heat shock method, this process is kept at a low temperature ensuring a non-denaturing condition for the proteins and preserves the native albumin structure and function.

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A2153 Bovine Serum Albumin lyophilized powder, ≥96% (agarose gel electrophoresis)
A4503 Bovine Serum Albumin lyophilized powder, ≥96% (agarose gel electrophoresis)
A6003 Bovine Serum Albumin lyophilized powder, essentially fatty acid free, ≥96% (agarose gel electrophoresis)
A9418 Bovine Serum Albumin lyophilized powder, BioReagent, suitable for cell culture, ≥96% (agarose gel electrophoresis)
A8806 Bovine Serum Albumin fatty acid free, low endotoxin, lyophilized powder, BioReagent, suitable for cell culture, ≥96% (agarose gel electrophoresis)
A4378 Bovine Serum Albumin lyophilized powder, 1× crystallized, ≥97% (agarose gel electrophoresis)
A7511 Bovine Serum Albumin lyophilized powder, essentially fatty acid free, ≥97% (agarose gel electrophoresis), 1X Crystallized
05471 Bovine Serum Albumin lyophilized powder, ≥97.0% (HPCE)
A5611 Bovine Serum Albumin Cohn Fraction V, lyophilized powder, ≥96% (agarose gel electrophoresis)
A8531 Bovine Serum Albumin
05470 Bovine serum albumin lyophilized powder, crystallized, ≥98.0% (GE)
A9205 Bovine Serum Albumin solution 30% in saline, fatty acid free, aseptically filled

Heat Shock Fractionated

The heat shock fractionation process of heat and pH adjustment relies on the addition of a protein stabilizer. This addition allows the albumin to tolerate the increased temperature of > 65°C. Most other plasma proteins denature and precipitate during this process yielding an albumin with increased purity.

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B2518 Albumin, Acetylated from bovine serum lyophilized powder, suitable for (for molecular biology)
B8894 Albumin, Acetylated from bovine serum 20 mg/mL in H2O, protease free, for molecular biology
A7906 Bovine Serum Albumin heat shock fraction, pH 7, ≥98%
A7030 Bovine Serum Albumin heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98%
A7888 Bovine Serum Albumin lyophilized powder, RIA grade, ≥96% (agarose gel electrophoresis)
A9647 Bovine Serum Albumin heat shock fraction, pH 7, ≥98%
A3294 Bovine Serum Albumin heat shock fraction, protease free, pH 7, ≥98%
A3059 Bovine Serum Albumin heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%
A8022 Bovine Serum Albumin lyophilized powder, ≥96% (agarose gel electrophoresis)
A3912 Bovine Serum Albumin lyophilized powder, ≥96% (agarose gel electrophoresis)
A3803 Bovine Serum Albumin lyophilized powder, essentially fatty acid free, ≥98% (agarose gel electrophoresis)
B4287 Bovine Serum Albumin BioReagent, for molecular biology
A4919 Bovine Serum Albumin heat shock fraction, protease free, low endotoxin, suitable for cell culture, pH 7, ≥98%
A3311 Bovine Serum Albumin powder, BioXtra
A9430 Bovine Serum Albumin heat shock fraction, low endotoxin, pH 7, ≥98%
A2934 Bovine Serum Albumin lyophilized powder, essentially globulin free, low endotoxin, ≥98% (agarose gel electrophoresis)
B6917 Bovine Serum Albumin lyophilized powder, suitable for (for molecular biology), Non-acetylated
A9543 Bovine Serum Albumin heat shock fraction, low endotoxin, pH 7, ≥98%
A9056 Bovine Serum Albumin lyophilized powder, Microbiologically tested, ≥96% (agarose gel electrophoresis)
A9085 Bovine Serum Albumin lyophilized powder, essentially IgG free, ≥96% (agarose gel electrophoresis)
A7517 Bovine Serum Albumin For use as a marker in SDS-PAGE
A1470 Bovine Serum Albumin low endotoxin, lyophilized powder, Cohn Analog, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)
A3902 Bovine Serum Albumin lyophilized powder, Vitamin B12 deficient
A3733 Bovine Serum Albumin lyophilized powder, ≥98% (agarose gel electrophoresis)
A3983 Bovine Serum Albumin lyophilized powder, essentially γ-globulin free, ≥98% (agarose gel electrophoresis)
A3608 Bovine Serum Albumin lyophilized powder, ≥96% (agarose gel electrophoresis)
A3858 Bovine Serum Albumin lyophilized powder, Protease, essentially free, ≥98% (agarose gel electrophoresis)
A7284 Bovine Serum Albumin solution 30%±2% in 0.85% sodium chloride, aseptically filled
A7409 Bovine Serum Albumin solution 35% in 0.85% sodium chloride, aseptically filled
A8577 Bovine Serum Albumin solution 30% in saline, protease free, aseptically filled
A7034 Bovine Serum Albumin solution 22% in saline, contains azide, aseptically filled
A8327 Bovine Serum Albumin solution 30%±2% in 0.85% sodium chloride, aseptically filled
B8667 Bovine Serum Albumin solution 20 mg/mL in H2O, low bioburden, protease free, for molecular biology
A1662 Bovine Serum Albumin solution 30%±2% in 0.85% sodium chloride, aseptically filled
A4628 Bovine Serum Albumin solution 5% in 0.85% sodium chloride, aseptically filled
A3174 Bovine Serum Albumin solution 30%±2% in 0.85% sodium chloride, aseptically filled, High avidity
A0336 Bovine Serum Albumin solution 30%±2% in 0.85% sodium chloride (essentially IgG free), aseptically filled, essentially IgG free
A7534 Bovine Serum Albumin solution 35% in saline, contains azide, aseptically filled
A3299 Bovine Serum Albumin solution 30% in 0.85% sodium chloride, aseptically filled
A7159 Bovine Serum Albumin solution 25% in Tyrode's buffer, aseptically filled
A1595 Bovine Serum Albumin solution 10% in DPBS, low endotoxin, fatty acid free, suitable for cell culture, sterile-filtered