Cerosomes Cytochrome P450 (CYP) Active Microsome Preparations

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Recombinant microsomal preparations have the advantage of a single cytochrome P450 isozyme activity. Use of a eukaryotic, yeast host allows efficient expression of the active isozyme with more accurate post-translational modification.

Cerosomes are prepared from Saccharomyces cervisiae expressing the human cytochrome P450 isozyme and human NADPH reductase and cytochrome b5 in some preparations.

The eukaryotic system provides an isozyme system with the following advantages:
  • Recombinant isozyme mimics the active, native human enzyme
  • Higher specific activity and better signal-to-noise ratio
  • Consistent and reproducible results

The cytochrome P450 isozymes are supplied in a solution containing 50 mM potassium phosphate, pH 7.4, 5% glycerol, 1 mM EDTA, and 1 mM PMSF.