Nanodisc Reagents for Membrane Protein Research

Nanodisc News: Read our article in Genetic Engineering News about the use of Nanodisc technology for Mass Spec Analysis of Intact Membrane Proteins

Nanodisc soluble lipid bilayer systems have proven to be a widely applicable means for rendering membrane pro­teins soluble in aqueous solutions in a native-like bilayer environment where they remain monodisperse and active.
The Nanodisc concept is derived from naturally occuring high density liporotein (HDL) particles. Our new membrane scaffold protein (MSP) constructs utilize the amphipathic properties found in Apolipoprotein A-1 (ApoA-1) to provide a multi-helical scaffold for spontaneous Nanodisc as­sembly.

Advantages of the Nanodisc Technology
  • Renders membrane proteins water soluble
  • Proteins retain native conformation and activity
  • Provides a highly stable micro-environment for your protein
  • Membrane proteins remain monodispersed in aqueous solutions
  • Offers improved particle uniformity over microsomes
  • Surrounds your protein a more native-like phospholipid bilayer
To incorporate a protein of interest into Nanodiscs, cholate-solubilized phospholipids are mixed with the membrane scaffold protein and a deter­gent-solubilized target membrane protein, everything is incubated together, the detergents are removed with Amberlite XAD-2 or by dialysis and the self-assembly takes place. For further details, check out our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

Just a few examples of active membrane-bound proteins that have been successfully incorporated into Nanodiscs are:

  • GPCRs
  • Cytochrome P450s
  • Bacteriorhodopsin
  • Coagulation Factors
  • Cholera Toxin
  • TAR Receptor
  • SecYEG
  • ABC Transporters