Chromatography Products for Nanodisc Preparations

The Nanodisc self-assembly process is initiated upon removal of the cholate detergent by dialysis or with Amberlite XAD-2.
Assembled Nanodiscs can be purified by HIS-Select affinity chro­matography using the 6-histidine tag on the MSP or a purification method appropriate for the target protein.
Subsequent fractionation using size-exclusion chromatography with Superdex can remove any unassembed components.

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20275 Amberlite® XAD®-2
P6611 HIS-Select® Nickel Affinity Gel
S6782 Superdex® Superdex®
200 Prep Grade