CF Dyes

CF Dyes: Enhanced Fluorophores with Novel Dye Chemistry

CF dyes are a series of highly water-soluble fluorescent dyes spanning the visible and near-infrared (near-IR) spectrum (Figure 1A and 1B) for labeling antibodies, proteins, nucleic acids, and other biomolecules. Developed by scientists using new breakthrough chemistries, the brightness, photostability, and color selection of CF dyes rival or exceed the quality of other commercial dyes as a result of rational dye design.
Achieve greater brightness, photostability, and sensitivity with CF dyes
Sigma currently offers 19 CF dyes spanning the visible and near-IR wavelengths, with additional colors in development. The CF dye product line includes reactive CF dyes, labeling kits, CF-labeled secondary antibodies, and other bioconjugates. This collection further expands Sigma’s broad range of carefully selected secondary antibodies and conjugates, allowing scientists to achieve greater sensitivity, brighter results, and better photostability in immunoassays (Figures 2A, 2B, and 2C).
To help researchers evaluate their secondary antibody products with complete peace of mind, Sigma is offering the Bioguarantee on all CF dye labeled antibody products.

CF Dye Labeled Secondary Antibodies

Product #

Description

Assay

Fluorescence

Add to Cart

SCJ4600001 CF 350 amine   λex 347 nm; λem 448 nm
λex 360 nm (Flow cytometry laser line)
λex 360 nm (Microscopy laser line)
SCJ4600002 CF 350 aminooxy   λex 347 nm; λem 448 nm
λex 360 nm (Flow cytometry laser line)
λex 360 nm (Microscopy laser line)
SCJ4600003 CF 350 hydrazide   λex 347 nm; λem 448 nm
λex 360 nm (Flow cytometry laser line)
λex 360 nm (Microscopy laser line)
SCJ4600004 CF 350 maleimide   λex 347 nm; λem 448 nm
λex 360 nm (Flow cytometry laser line)
λex 360 nm (Microscopy laser line)
SCJ4600005 CF 350 succinimidyl ester   λex 347 nm; λem 448 nm
λex 360 nm (Flow cytometry laser line)
λex 360 nm (Microscopy laser line)
SCJ4600007 CF 405M aminooxy   λex 360, 405 or 407 nm (Flow cytometry laser line)
λex 405 nm (Microscopy laser line)
λex 408 nm; λem 450 nm
SCJ4600008 CF 405M maleimide   λex 360, 405 or 407 nm (Flow cytometry laser line)
λex 405 nm (Microscopy laser line)
λex 408 nm; λem 451 nm
SCJ4600010 CF 405S amine   λex 360, 405 or 407 nm (Flow cytometry laser line)
λex 404 nm; λem 428 nm
λex 405 nm (Microscopy laser line)
SCJ4600011 CF 405S aminooxy   λex 360, 405 or 407 nm (Flow cytometry laser line)
λex 404 nm; λem 429 nm
λex 405 nm (Microscopy lase line)
SCJ4600012 CF 405S maleimide   λex 360, 405 or 407 nm (Flow cytometry laser line)
λex 404 nm; λem 430 nm
λex 405 nm (Microscopy laser line)
SCJ4600013 CF 405S succinimidyl ester ≥90% (TLC)
λex 360, 405 or 407 nm (Flow cytometry laser line)
λex 404 nm; λem 431 nm
λex 405 nm (Microscopy laser line)
SCJ4600014 CF 488A amine ≥90% (HPLC)
≥90% (TLC)
λex 488 nm (Flow cytometry laser line)
λex 488 nm (Microscopy laser line)
λex 490 nm; λem 515 nm
SCJ4600017 CF 488A aminooxy ≥90% (HPLC)
λex 488 nm (Flow cytometry laser line)
λex 488 nm (Microscopy laser line)
λex 490 nm; λem 515 nm
SCJ4600015 CF 488A hydrazide ≥90% (HPLC)
λex 488 nm (Flow cytometry laser line)
λex 488 nm (Microscopy laser line)
λex 490 nm; λem 515 nm
SCJ4600016 CF 488A maleimide ≥90% (TLC)
λex 488 nm (Flow cytometry laser line)
λex 488 nm (Microscopy laser line)
λex 490 nm; λem 515 nm
SCJ4600018 CF 488A succinimidyl ester   λex 488 nm (Flow cytometry laser line)
λex 488 nm (Microscopy laser line)
λex 490 nm; λem 515 nm
SCJ4600019 CF 555 amine   λex 488 or 532 nm (Flow cytometry laser line)
λex 488 or 532 nm (Microscopy laser line)
λex 555 nm; λem 565 nm
SCJ4600020 CF 555 hydrazide   λex 488 or 532 nm (Flow cytometry laser line)
λex 488 or 532 nm (Microscopy laser line)
λex 555 nm; λem 565 nm
SCJ4600021 CF 555 maleimide ≥90% (TLC)
λex 488 or 532 nm (Flow cytometry laser line)
λex 488 or 532 nm (Microscopy laser line)
λex 555 nm; λem 565 nm
SCJ4600022 CF 555 succinimidyl ester ≥90% (TLC)
λex 488 or 532 nm (Flow cytometry laser line)
λex 488 or 532 nm (Microscopy laser line)
λex 555 nm; λem 565 nm
SCJ4600023 CF 568 amine   λex 488,532 or 561 nm (Flow cytometry laser line)
λex 562 nm; λem 583 nm
λex 568 nm (Microscopy laser line)
SCJ4600024 CF 568 aminooxy   λex 488,532 or 561 nm (Flow cytometry laser line)
λex 562 nm; λem 583 nm
λex 568 nm (Microscopy laser line)
SCJ4600025 CF 568 hydrazide ≥90% (HPLC)
≥90% (TLC)
λex 488,532 or 561 nm (Flow cytometry laser line)
λex 562 nm; λem 583 nm
λex 568 nm (Microscopy laser line)
SCJ4600026 CF 568 maleimide ≥90% (TLC)
λex 488,532 or 561 nm (Flow cytometry laser line)
λex 562 nm; λem 583 nm
λex 568 nm (Microscopy laser line)
SCJ4600027 CF 568 succinimidyl ester ≥90% (TLC)
λex 488, 532 or 561 nm (Flow cytometry laser line)
λex 562 nm; λem 583 nm
λex 568 nm (Microscopy laser line)
SCJ4600028 CF 594 amine   λex 532 or 561 nm (Flow cytometry laser line)
λex 593 nm; λem 614 nm
λex 594 nm (Microscopy laser line)
SCJ4600032 CF 594 aminooxy   λex 532 or 561 nm (Flow cytometry laser line)
λex 593 nm; λem 614 nm
λex 594 nm (Microscopy laser line)
SCJ4600029 CF 594 hydrazide ≥90% (HPLC)
≥90% (TLC)
λex 532 or 561 nm (Flow cytometry laser line)
λex 593 nm; λem 614 nm
λex 594 nm (Microscopy laser line)
SCJ4600030 CF 594 maleimide ≥90% (TLC)
λex 532 or 561 nm (Flow cytometry laser line)
λex 593 nm; λem 614 nm
λex 594 nm (Microscopy laser line)
SCJ4600031 CF 594 succinimidyl ester ≥90% (TLC)
λex 532 or 561 nm (Flow cytometry laser line)
λex 593 nm; λem 614 nm
λex 594 nm (Microscopy laser line)
SCJ4600033 CF 620R maleimide ≥90% (TLC)
λex 617 nm; λem 639 nm
λex 633 or 635 nm (Flow cytometry laser line)
λex 633 or 635 nm (Microscopy laser line)
SCJ4600034 CF 620R succinimidyl ester ≥90% (TLC)
λex 617 nm; λem 639 nm
λex 633 or 635 nm (Flow cytometry laser line)
λex 633 or 635 nm (Microscopy laser line)
SCJ4600035 CF 633 amine   λex 630 nm; λem 650 nm
λex 633 or 635 nm (Flow cytometry laser line)
λex 633 or 635 nm (Microscopy laser line)
SCJ4600036 CF 633 aminooxy   λex 630 nm; λem 650 nm
λex 633 or 635 nm (Flow cytometry laser line)
λex 633 or 635 nm (Microscopy laser line)
SCJ4600037 CF 633 hydrazide ≥90% (HPLC)
λex 630 nm; λem 650 nm
λex 633 or 635 nm (Flow cytometry laser line)
λex 633 or 635 nm (Microscopy laser line)
SCJ4600038 CF 633 maleimide   λex 630 nm; λem 650 nm
λex 633 or 635 nm (Flow cytometry laser line)
λex 633 or 635 nm (Microscopy laser line)
SCJ4600039 CF 633 succinimidyl ester ≥90% (TLC)
λex 630 nm; λem 650 nm
λex 633 or 635 nm (Flow cytometry laser line)
λex 633 or 635 nm (Microscopy laser line)
SCJ4600040 CF 640R amine ≥90% (HPLC)
λex 633, 635 or 640 nm (Flow cytometry laser line)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 642 nm; λem 662 nm
SCJ4600041 CF 640R aminooxy ≥90% (TLC)
λex 633, 635 or 640 nm (Flow cytometry laser line)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 642 nm; λem 662 nm
SCJ4600042 CF 640R hydrazide   λex 633, 635 or 640 nm (Flow cytometry laser line)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 642 nm; λem 662 nm
SCJ4600043 CF 640R maleimide ≥90% (TLC)
λex 633, 635 or 640 nm (Flow cytometry laser line)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 642 nm; λem 662 nm
SCJ4600044 CF 640R succinimidyl ester ≥90% (TLC)
λex 633, 635 or 640 nm (Flow cytometry laser line)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 642 nm; λem 662 nm
SCJ4600045 CF 647 amine   λex 633, 635 or 640 nm (Flow cytometry laser line)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 650 nm; λem 665 nm
SCJ4600046 CF 647 hydrazide   λex 633, 635 or 640 nm (Flow cytometry laser line)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 650 nm; λem 665 nm
SCJ4600047 CF 647 maleimide ≥90% (TLC)
λex 633, 635 or 640 nm (Flow cytometry laser line)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 650 nm; λem 665 nm
SCJ4600048 CF 647 succinimidyl ester ≥90% (TLC)
λex 633, 635 or 640 nm (Flow cytometry laser line)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 650 nm; λem 665 nm
SCJ4600049 CF 660C maleimide ≥90% (TLC)
λex 633, 635 or 640 nm (Flow cytometry lab)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 667 nm; λem 685 nm
SCJ4600050 CF 660C succinimidyl ester ≥90% (TLC)
λex 633, 635 or 640 nm (Flow cytometry laser line)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 667 nm; λem 685 nm
SCJ4600051 CF 660R aminooxy   λex 633, 635 or 640 nm (Flow cytometry laser line)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 663 nm; λem 682 nm
SCJ4600052 CF 660R maleimide ≥90% (TLC)
λex 633, 635 or 640 nm (Flow cytometry laser line)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 663 nm; λem 682 nm
SCJ4600053 CF 660R succinimidyl ester   λex 633, 635 or 640 nm (Flow cytometry laser line)
λex 633, 635 or 640 nm (Microscopy laser line)
λex 663 nm; λem 682 nm
SCJ4600054 CF 680 maleimide ≥90% (TLC)
λex 680 or 685 nm (Flow cytometry laser line)
λex 680 or 685 nm (Microscopy laser line)
λex 681 nm; λem 698 nm
SCJ4600055 CF 680 succinimidyl ester ≥90% (TLC)
λex 680 or 685 nm (Flow cytometry laser line)
λex 680 or 685 nm (Microscopy laser line)
λex 681 nm; λem 698 nm
SCJ4600056 CF 680R aminooxy   λex 680 or 685 nm (Flow cytometry laser line)
λex 680 or 685 nm (Microscopy laser line)
λex 680 nm; λem 701 nm
SCJ4600057 CF 680R maleimide ≥90% (TLC)
λex 680 or 685 nm (Flow cytometry laser line)
λex 680 or 685 nm (Microscopy laser line)
λex 680 nm; λem 701 nm
SCJ4600058 CF 680R succinimidyl ester ≥90% (TLC)
λex 680 or 685 nm (Flow cytometry laser line)
λex 680 or 685 nm (Microscopy laser line)
λex 680 nm; λem 701 nm
SCJ4600059 CF 750 succinimidyl ester ≥90% (TLC)
λex 680 or 685 nm (Flow cytometry laser line)
λex 680 or 685 nm (Microscopy laser line)
λex 755 nm; λem 777 nm
SCJ4600060 CF 770 succinimidyl ester ≥90% (TLC)
λex 770 nm; λem 797 nm
λex 785 nm (Flow cytometry laser line)
λex 785 nm (Microscopy laser line)
SCJ4600061 CF 790 succinimidyl ester   λex 784 nm; λem 806 nm
λex 785 nm (Flow cytometry laser line)
λex 785 nm (Microscopy laser line)