Starch and Pectin Hydrolysis

Enzymatic hydrolysis of starch-based biomass to yield monomeric glucose is often a multi-enzyme process and can involve α-amylase, β-amylase, amyloglucosidase, isoamylase and α-glucosidase.
  • α-Amylase catalyzes the endohydrolysis of α−(1→4)-D-glucosidic linkages in polysaccharides containing three or more α−(1→4)-linked D-glucose units.
  • β-Amylase catalyzes the exohydrolysis of α−(1→4)-D-glucosidic linkages in polysaccharides resulting in the successive liberation of maltose units from the non-reducing ends of the chains.
  • Amyloglucosidase (glucoamylase) catalyzes the hydrolysis of terminal α−(1→4)-D-glucose residues successively from the non-reducing ends of maltooligo- and polysaccharides with release of β-D-glucose. Most forms of the enzyme can rapidly hydrolyze α−(1→6)-D-glucosidic bonds when the next bond in the sequence is 1→4- and some preparations of this enzyme hydrolyze 1→6- and 1→3-α-D-glucosidic bonds in other polysaccharides.
  • Isoamylase catalyzes the hydrolysis of α−(1→6)-D-glucosidic branch linkages in amylopectin and β-limit dextrins.
α-Glucosidase catalyzes the hydrolysis of terminal 1→4-linked α-D-glucose residues successively from the non-reducing ends of maltooligo- and to a lesser extent polysaccharides with release of β-D-glucose. Most forms of the enzyme can slowly hydrolyze α−(1→6)-D-glucosidic bonds.

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A8220 α-Amylase from Aspergillus oryzae aqueous solution, ≥800 FAU/g Green Alternative
A9857 α-Amylase from Aspergillus oryzae powder, ≥150 units/mg protein (biuret) Green Alternative
A7595 α-Amylase from Bacillus amyloliquefaciens liquid, ≥250 units/g Green Alternative
A3403 α-Amylase from Bacillus licheniformis Type XII-A, saline solution, ≥500 units/mg protein (biuret) Green Alternative
A4551 α-Amylase from Bacillus licheniformis lyophilized powder, 500-1,500 units/mg protein, 93-100% (SDS-PAGE) Green Alternative
A9972 α-Amylase from human pancreas lyophilized powder, ≥100 units/mg protein Green Alternative
A1031 α-Amylase from human saliva Type XIII-A, lyophilized powder, 300-1,500 units/mg protein Green Alternative
A0521 α-Amylase from human saliva Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein Green Alternative
A3176 α-Amylase from porcine pancreas Type VI-B, ≥10 units/mg solid Green Alternative
A6255 α-Amylase from porcine pancreas DFP Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280) Green Alternative
A4268 α-Amylase from porcine pancreas Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280) Green Alternative
A7130 β-Amylase from barley Type II-B, 20-80 units/mg protein (biuret) Green Alternative
A7005 β-Amylase from sweet potato Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280) Green Alternative
A3306 α-Amylase, heat-stable solution, for use in Total Dietary Fiber Assay, TDF-100A Green Alternative
A2986 Amylase, Maltogenic from Bacillus sp. Green Alternative
G3651 α-Glucosidase from Bacillus stearothermophilus lyophilized powder, ≥50 units/mg protein
G9259 α-Glucosidase from rice Type V, ammonium sulfate suspension, 40-80 units/mg protein
G0660 α-Glucosidase from Saccharomyces cerevisiae recombinant, expressed in proprietary host, lyophilized powder, ≥100 units/mg protein
G5003 α-Glucosidase from Saccharomyces cerevisiae Type I, lyophilized powder, ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)
P2401 Pectinase from Rhizopus sp. powder, 400-800 units/g solid
P3026 Pectolyase from Aspergillus japonicus lyophilized powder, ≥0.3 units/mg solid