Isotype Controls

Cell typing with monoclonal antibodies to CD antigens in flow cytometry or other immunological applications can give false positives in the absence of proper controls. Sigma recommends using a non-reactive immunoglobulin of the same isotype and concentration to detect non-specific binding. We offer purified monoclonal mouse immunoglobulins of isotypes IgG1, IgG2a, IgG2b, and IgM. Each is available both unlabeled and conjugated to fluorescein isothiocyanate(FITC). Mouse IgG1 conjugated to phycoerythrin is also available.

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12-370 Normal Rabbit IgG Normal Rabbit IgG Polyclonal Antibody control validated for use in Immunoprecipitation & Western Blotting.
12-371 Normal Mouse IgG Normal Mouse IgG Polyclonal Antibody control validated for use in Immunoprecipitation & Western Blotting.
12-487 Normal Mouse IgG, FITC conjugate Normal Mouse IgG Polyclonal Antibody control validated for use in Immunofluorescence.
12-515 Normal Sheep IgG Normal Sheep IgG Polyclonal Antibody control validated for use in Western Blotting.
16-237 Normal Rabbit IgG, Alexa Fluor® 488 conjugate Normal Rabbit IgG Polyclonal Antibody control validated for use in Immunofluorescence.
16-240 Normal Mouse IgG, Alexa Fluor® 488 conjugate Normal Mouse IgG Polyclonal Antibody control validated for use in Immunofluorescence.
400109 Human IgA₁, Myeloma
400110 Human IgA₂, Myeloma
400120 Human IgG₁, Myeloma
400122-M Human IgG₂, Myeloma
400126 Human IgG₄, Myeloma
401104 Human IgG, Fc Fragment, Plasma
401108 Human IgM, Myeloma
401111 Mouse IgG, Serum
401122 Mouse IgG₁, Myeloma
401152 Human IgE, Myeloma This Human IgE, Myeloma is validated for use as an Immunoassay standard.
401799 Human IgM, Plasma
AC146 Normal Chicken IgY Antibody, purified | AC146
AG100 Human IgG Fc Control Protein, recombinant protein
AG30P Human IgE, myeloma
AG502-M Human IgG1 kappa
AG504 Human IgG2 kappa
AG506 Human IgG3 kappa
AG508 Human IgG4 kappa
AG711-M Human IgG, Polyclonal
AG714 Human IgG, Fc Fragment
AG722 Human IgM
AP132-M Goat Anti-Rabbit IgG, Affinity Purified (1mg)
CBL610 Mouse IgG1 Negative Control Antibody, clone 1E2.2 Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry.
MABC002 Mouse IgG1 Negative Control, clone Ci4 Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
MABC002F Mouse IgG1 Negative Control, clone Ci4, FITC conjugate Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
MABC002H Mouse IgG1 Negative Control, clone Ci4, PE conjugate Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry.
MABC004 Mouse IgG2a Negative Control, clone GC270 Mouse IgG2a Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
MABC004F Mouse IgG2a Negative Control, clone GC270, FITC conjugate Mouse IgG2a Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
MABC006 Mouse IgG2b Negative Control, clone GC198 Mouse IgG2b Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
MABC006F Mouse IgG2b Negative Control, clone GC198, FITC conjugate Mouse IgG2b Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
MABC008 Mouse IgM Negative Control, clone GC323 Mouse IgM Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
MABC016H Mouse IgG2a Negative Control, clone GC208, PE conjugate Mouse IgG2a Negative Control Monoclonal Antibody validated for use in Flow Cytometry.
PP100 IgG1 Antibody, Mouse Mouse IgG1 Monoclonal Antibody control validated for use in ELISA.
PP102 IgG2a Antibody, Mouse Mouse IgG2a Monoclonal Antibody control validated for use in ELISA.
PP40 IgG, Goat Goat IgG Polyclonal Antibody control validated for use in ELISA.
PP50 IgM, Mouse Mouse IgM Polyclonal Antibody control validated for use in ELISA.
PP541 Mouse IgG Purified, No Azide (1gm)
PP54 IgG, Mouse Mouse IgG Polyclonal Antibody control validated for use in ELISA.
PP64 IgG, Rabbit Rabbit IgG Polyclonal Antibody validated for use in ELISA.
PP68 IgG, Rat Rat IgG Polyclonal Antibody control validated for use in ELISA.
F6397 IgG1−FITC Isotype Control from murine myeloma clone MOPC 21, purified immunoglobulin, buffered aqueous solution
M5284 IgG1 Isotype Control from murine myeloma clone MOPC 21, purified immunoglobulin, buffered aqueous solution
P4685 IgG1−PE Isotype Control from murine myeloma clone MOPC 21, purified immunoglobulin, buffered aqueous solution
F6522 IgG2a−FITC Isotype Control from murine myeloma clone UPC-10, purified immunoglobulin, buffered aqueous solution
M5909 IgM Isotype Control from murine myeloma clone MOPC 104E, 200 μg/mL, buffered aqueous solution, purified immunoglobulin
M5409 Mouse IgG2a Isotype Control from murine myeloma clone UPC-10, purified immunoglobulin, buffered aqueous solution