Heparinase Specificities

Heparinase selectively cleaves sulfated glycans containing a(1-4) glycosidic linkages between the glucosamine and uronic acid residues in the heparin polymer. The cleavage proceeds via an elimination reaction, resulting in the formation of oligosaccharides containing unsaturated uronic acid residues (double bond between C4 and C5). These cleavage products can be detected by UV spectroscopy (232 nm).