Protease Inhibitor Cocktails

Don't Lose Your Proteins During Sample Prep

Protease inhibitors are tremendously valuable and useful reagents for researchers who want to inhibit general degradation of proteins in tissue or cell extracts by endogenous proteases, or to investigate particular processes that involve blocking the activity of specific proteases.

Four main classes of proteolytic enzymes have been routinely utilized to describe proteases. The serine proteases are probably the best characterized. This class of proteases includes trypsin, chymotrypsin and elastase. The cysteine protease class includes papain, calpain and lysosomal cathepsins. Aspartic proteases include pepsin and rennin. Metallo-proteases include thermolysin and carboxypeptidase A.

During isolation and characterization, one or all four classes of proteases may pose a threat to the fate of a protein. Broad spectrum protease inhibitors and mixtures, or Cocktails, have been developed to help protect the integrity of proteins against multiple classes of proteases.

Sigma-Aldrich offers the broadest range of well-established individual protease inhibitors and protease inhibitor cocktails. The protease inhibitor cocktails (mixtures) have been developed to safeguard the integrity of proteins during extraction and purification, to protect your proteins during sample prep. In addition to the Sigma-Aldrich line of protease inhibitor cocktails, our product offerings now include Roche’s cOmplete protease inhibitor cocktail tablets.

  • Optimized to maintain and preserve protein functionality following cell lysis
  • Available in powder, solution, or tablet form
  • Inhibits proteases in almost any tissue or cell
  • Specific formulations optimized for particular applications

Our Inhibitor Cocktails have a stellar performance record, as seen in reference citations from our customers.
View additional citations for each individual protease inhibitor cocktail product under the "Peer-Reviewed Papers" tab of each product’s detail page.

See also Enzyme Inhibitors→Protease Inhibitors.

cOmplete is a trademark of Roche. For life science research only. Not for use in diagnostic procedures.

Product #

Product Name

Sample Type

Specificity of Inhibition

Components

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SRE0055 Protease Inhibitor Cocktail Powder Reagents designed and manufactured under cGMP controls suitable for use in an IVD application. Reagents designed and manufactured under cGMP controls suitable for use in an IVD application. nhibits serine, cysteine, and metallo-proteases.
1 bottle in serum bottle
Components
AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin
S8830 SIGMAFAST Protease Inhibitor Cocktail Tablets, EDTA-Free for use in purification of Histidine-tagged proteins for use in purification of Histidine-tagged proteins Inhibits serine, cysteine, aspartic, and metalloproteases.
AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon
Leupeptin
Aprotinin
S8820 SIGMAFAST Protease Inhibitor Tablets For General Use For General Use Inhibits serine, cysteine, aspartic, and metalloproteases.
AEBSF, 2 mM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM
PHOSS-RO PhosSTOP   Protects proteins against dephosphorylation. Inhibits phosphatase classes such as acid and alkaline phosphatases, as well as serine/threonine (PP1, PP2A, and PP2B) and tyrosine protein phosphatases (PTP) in bacterial, mammalian, yeast, and plant extracts.
 
P9599 Protease Inhibitor Cocktail for plant cell and tissue extracts, DMSO solution for plant cell and tissue extracts, DMSO solution Inhibits serine, cysteine, and aspartic proteases, metalloproteases, and aminopeptidases
AEBSF
Bestatin
E-64
Leupeptin
Pepstatin A
1,10-Phenanthroline
P8849 Protease Inhibitor Cocktail for use in purification of Histidine-tagged proteins, DMSO solution for use in purification of Histidine-tagged proteins, DMSO solution Inhibits serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.
AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon
P8340 Protease Inhibitor Cocktail for use with mammalian cell and tissue extracts, DMSO solution for use with mammalian cell and tissue extracts, DMSO solution Inhibits serine, cysteine, and acid proteases, and aminopeptidases.
AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
P8215 Protease Inhibitor Cocktail for use with fungal and yeast extracts, DMSO solution for use with fungal and yeast extracts, DMSO solution Inhibits serine, cysteine, aspartic, and metalloproteases.
AEBSF, 100 mM
E-64, 1.4 mM
Pepstatin A, 2.2 mM
1,10-Phenanthroline, 500 mM
P2714 Protease Inhibitor Cocktail powder for general use, lyophilized powder for general use, lyophilized powder Inhibits serine, cysteine, and metallo-proteases.
AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin
P1860 Protease Inhibitor Cocktail for use in tissue culture media, DMSO solution for use in tissue culture media, DMSO solution Inhibits serine, cysteine, aspartic proteases, and aminopeptidases
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
MSSAFE MS-SAFE Protease and Phosphatase Inhibitor   Protease inhibitors: serine, cysteine and aspartic proteases, and metalloproteases
Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases
Protease inhibitors:
• Bestatin hydrochloride
• Leupeptin
• Phosphoramidon disodium salt
• Pepstatin A
• Elastatinal
• Aprotinin
• Nafamostat mesylate
• Antipain
Phosphatase inhibitors
• Okadaic acid
• Sodium fluoride
• Sodium orthovanadate
• Bromotetramisole oxalate
Fillers
• β-lactose
• DL-leucine

I3911 Protease Inhibitor Cocktail, Animal Component Free for general use, lyophilized powder for general use, lyophilized powder Inhibits serine, cysteine, aspartic, and metalloproteases.
AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin
I3786 Protease Inhibitor Cocktail, Animal Component Free for use with mammalian cell and tissue extract, DMSO solution for use with mammalian cell and tissue extract, DMSO solution Inhibits serine, cysteine, aspartic proteases, and aminopeptidases.
AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
COUL-RO cOmplete ULTRA Tablets, glass vials Protease Inhibitor Cocktail Tablets provided in glass vials Tablets provided in glass vials    
COUEDTAF-RO cOmplete ULTRA Tablets, EDTA-free, glass vials Protease Inhibitor Cocktail Tablets provided in glass vials Tablets provided in glass vials    
COEDTAF-RO cOmplete, EDTA-free Protease Inhibitor Cocktail Tablets provided in glass vials Tablets provided in glass vials    
CO-RO cOmplete Protease Inhibitor Cocktail Tablets provided in glass vials Tablets provided in glass vials Mixture of several protease inhibitors with broad inhibitory specificity. For the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.
 
11836170001 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Protease Inhibitor Cocktail Tablets provided in a glass vial, Tablets provided in a glass vial Protease Inhibitor Cocktail Tablets provided in a glass vial, Tablets provided in a glass vial Mixture of several protease inhibitors. For the inhibition of serine, cysteine, but not metalloproteases.
 
11836153001 cOmplete, Mini Protease Inhibitor Cocktail Tablets provided in a glass vial Tablets provided in a glass vial Mixture of several protease inhibitors with broad inhibitory specificity. For the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.
 
05892970001 cOmplete ULTRA Tablets, Mini, EASYpack Protease Inhibitor Cocktail Tablets supplied in foil blister packs. Tablets supplied in foil blister packs.    
05892791001 cOmplete ULTRA Tablets, Mini, EDTA-free, EASYpack Protease Inhibitor Cocktail Tablets supplied in foil blister packs. Tablets supplied in foil blister packs.    
04693159001 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Tablets provided in EASYpacks Tablets provided in EASYpacks Mixture of several protease inhibitors. For the inhibition of serine, cysteine, but not metalloproteases.
 
04693132001 cOmplete, EDTA-free Protease Inhibitor Cocktail Tablets provided in EASYpacks Tablets provided in EASYpacks Mixture of several protease inhibitors. For the inhibition of serine, cysteine, but not metalloproteases.
 
04693124001 cOmplete, Mini Protease Inhibitor Cocktail Tablets provided in EASYpacks Tablets provided in EASYpacks Mixture of several protease inhibitors with broad inhibitory specificity. For the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.
 
04693116001 cOmplete Protease Inhibitor Cocktail Tablets provided in EASYpacks Tablets provided in EASYpacks