Restriction Enzymes

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Restriction Enzyme

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R6142 Acc I from Acinetobacter calcoaceticus Restriction Enzyme Recognition Sequence: 5′-GT/(A,C)(G,T)AC-3′
Cutting results: a 2-10-fold Acc I overdigestion of 1 μg pBR322 DNA substrate results in 100% cutting
Heat inactivation: Activity retained after 10 minutes at 65 °C. Five-fold enhanced activity occurs at 55°C. Enzyme inactivated at 80 °C for 20 minutes.
R6885 Alu I from Arthrobacter luteus Restriction Enzyme Recognition Sequence: 5′-AG/CT-3′
Ligation and recutting results: After 2-10-fold Alu I overdigestion of 1 μg λ DNA substrate, results in 95% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
R4258 Apa I from Acetobacter pasteurianus Restriction Enzyme Recognition Sequence: 5′-GGGCC/C-3′
Cutting results: a 2-10-fold Apa I overdigestion of Ad-2 DNA substrate results in 100% cutting.
Heat inactivation: 65 °C for 15 minutes.
R0260 BamH I from Bacillus amyloliquefaciens H Restriction Enzyme Recognition sequence: 5′-G/GATCC-3′
Cutting results: a 2-10-fold Bam HI overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 60 °C for 15 minutes.
Star activity: To prevent star activity, avoid suboptimal reaction conditions containing low salt concentration, high glycerol (>5%) and high pH 8.0.
R8631 Bcl I from Bacillus caldolyticus Restriction Enzyme Recognition sequence: 5′-T/GATCA-3′
Cutting results: a 2-10-fold Bcl I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: This enzyme cannot be heat inactivated at 65 °C for 15 minutes.
R6377 Bgl II from Bacillus licheniformis Restriction Enzyme Recognition sequence: 5′-A/GATCT-3′
Cutting results: a 2-10-fold Bgl II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat Inactivation: This enzyme cannot be heat inactivated.
R3131 Bln I from Brevibacterium linens buffered aqueous glycerol solution Recognition sequence: 5′-C/CTAGG-3′
Ligation and recutting results: After 2-10-fold Bln I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >70% of fragments can be ligated, and >90% recut.
Heat inactivation:. This enzyme is not heat inactivated at 65 °C for 15 minutes.
R3635 Bsm I from Bacillus stearothermophilus NUB 36 Restriction Enzyme Recognition sequences: 5′-GAATGCN/-3′ 3′-CTTAC/GN 5
Ligation and recruiting results: After 2-10-fold Bsm I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Heat inactivation for Bsm I is not available.
R4253 BstE II from Bacillus stearothermophilus ET Restriction Enzyme Recognition sequence: 5′-G/GTNACC-3′
Cutting results: a 2-10-fold BstE II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: This enzyme cannot be inactivated at 65 °C for 15 minutes.
R1761 Cfo I from Clostridium formicoaceticum Restriction Enzyme Recognition Sequence: 5′-GCG/C-3′
Cutting results: a 2-10-fold Cfo I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Not complete at 65 °C for 10 minutes.
R7763 Cla I from Caryophanon latum L Restriction Enzyme Recognition sequence: 5′-AT/CGAT-3′
Cutting results: a 2-10-fold Cla I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Enzyme is not inactivated at 65 °C for 20 minutes.
R4256 Dde I from Desulfovibrio desulfuricans strain Norway Restriction Enzyme Recognition sequence: 5′-C/TNAG-3′
Ligation and recutting results: After 2-10-fold Dde I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Completed after 65 °C incubation for 20 minutes.
Comment: Single-stranded DNA cleaves slowly.
R8381 Dpn I from Diplococcus pneumoniae Restriction Enzyme Recognition sequence: 5′-GmA/TC-3′
Ligation and recutting results: After 2-10-fold Dpn I overdigestion of 1 μg pBR322 DNA substrate, results in 100% cutting, >30% of fragments can be ligated, and >90% recut.
Heat inactivation: 75 °C for 15 minutes.
R4381 Dra I from Deinococcus radiophilus Restriction Enzyme Recognition sequence: 5′-TTT/AAA-3′
Cutting results: a 2-10-fold Dra I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 20 minutes.
R3884 EclX I from Enterobacter cloacae 590 Restriction Enzyme Recognition sequence: 5′-C/GGCCG-3′
Ligation and recutting results: After 2-10-fold EclX I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 20 minutes.
R6265 EcoR I from Escherichia coli BS5 Restriction Enzyme Recognition sequence: 5′-G/AATTC-3′
Cutting results: a 2-10-fold EcoR I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 20 minutes.
R2756 EcoR V from Escherichia coli buffered aqueous glycerol solution Recognition sequence: 5′-GAT/ATC-3′
Ligation and recutting results: After 2-10-fold Eco RV overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
R5628 Hae III from Haemophilus aegyptius Restriction Enzyme Recognition sequence: 5′-GG/CC-3′
Ligation and recutting results: After 2-10-fold Hae III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >50% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
R1137 Hind III from Haemophilus influenzae Restriction Enzyme Recognition sequence: 5′-A/AGCTT-3′
Cutting results: 2-10-fold Hind III overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes
R8507 Hpa I from Haemophilus parainfluenzae Restriction Enzyme Recognition sequence: 5′-GTT/AAC-3′
Ligation and recutting results: After 2-10-fold Hpa I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Not completely inactivated at 65 °C for 15 minutes.
R0629 Hpa II from Haemophilus parainfluenzae buffered aqueous glycerol solution Recognition sequence: 5′-C/CGG-3′
Ligation and recutting results: After 2-10-fold Hpa II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 20 minutes.
R4134 Ksp I from Kluyvera sp. Restriction Enzyme Recognition sequence: 5′-CCGC/GG-3′
Ligation and recutting results: After 2-10-fold Ksp I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
R4506 Msp I from Moraxella sp. buffered aqueous glycerol solution Recognition sequence: 5′-C/CGG-3′
Ligation and recutting results: After 2-10-fold Msp I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
R1632 Mva I from Micrococcus varians Rfl 19 Restriction Enzyme Recognition sequence: 5′-CC/(A,T)GG-3′
Ligation and recutting results: After 2-10-fold Mva I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut
Heat inactivation: Inactivated at 60 °C for 15 minutes.
R8761 Nco I from Nocardia corallina Restriction Enzyme Recognition sequence: 5′-C/CATGG-3′
Cutting results: a 2-10-fold Nco I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.
R5509 Nde I from Neisseria denitrificans Restriction Enzyme Recognition sequence: 5′-CA/TATG-3′
Cutting results: a 2-10-fold Nde I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 60 °C for 15 minutes.
R5634 Nhe I from Neisseria mucosa heidelbergensis Restriction Enzyme Recognition sequence: 5′-G/CTAGC-3′
Cutting results: a 2-10-fold Nhe I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
R8506 Not I from Nocardia otidiscaviarum Restriction Enzyme Recognition sequence: 5′-GC/GGCCGC-3′
Cutting results: a 2-10-fold Not I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.
R5884 Nsi I from Neisseria sicca Restriction Enzyme Recognition sequence: 5′-ATGCA/T-3′
Heat inactivation: Inactivated at 65 °C for 20 minutes.
R7023 Pst I from Providencia stuartii Restriction Enzyme Recognition sequence: 5′-CTGCA/G-3′
Cutting results: a 2-10-fold Pst I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
R1508 Pvu I from Proteus vulgaris Restriction Enzyme Recognition sequence: 5′-CGAT/CG-3′
Cutting results: A 2-10-fold Pvu I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
R2631 Pvu II from Proteus vulgaris Restriction Enzyme Recognition sequence: 5′-CAG/CTG-3′
Cutting results: a 2-10-fold Pvu II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Activity not completely destroyed at 65 °C for 15 minutes.
R4756 Rsa I from Rhodopseudomonas sphaeroides Restriction Enzyme Recognition sequence: 5′-GT/AC-3′
Cutting results: a 2-10-fold Rsa I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 20 minutes.
R5268 Sac I from Streptomyces achromogenes Restriction Enzyme Recognition sequence: 5′-GAGCT/C-3′
Cutting results: a 2-10-fold Sac I overdigestion of 1 μg λ Hind III DNA substrate results in 100% cutting
Heat inactivation : Inactivated at 65 °C for 15 minutes.
R0754 Sal I from Streptomyces albus G Restriction Enzyme Recognition sequence: 5′-G/TCGAC-3′
Cutting results: a 2-10-fold Sal I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.
R0762 Sau3A I from Staphylococcus aureus Restriction Enzyme Recognition sequence: 5′-/GATC-3′
Cutting results: A 2-10-fold Sau3A I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: 65 °C for 20 minutes.
R5007 Sca I from Streptomyces caespitosus Restriction Enzyme Recognition sequence: 5′-AGT/ACT-3′
Ligation and recutting results: After 2-10-fold Sca I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
R8256 Sfi I from Streptomyces fimbriatus Restriction Enzyme Recognition sequence: 5′-GGCC(N)4/NGGCC-3′
Cutting results: a 2-10-fold Sfi I overdigestion of 1 μg Ad-2 DNA substrate results in 100% cutting
R4503 Sma I from Serratia marcescens Sb Restriction Enzyme Recognition sequence: 5′-CCC/GGG-3′
Cutting results: a 2-10-fold Sma I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes.
R5257 Spe I from Sphaerotilus sp. Restriction Enzyme Recognition sequence: 5′-A/CTAGT-3′
Cutting results: a 2-10-fold Spe I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
R7135 Sph I from Streptomyces phaeochromogenes Restriction Enzyme Recognition sequence: 5′-GCATG/C-3′
Cutting results: A 2-10-fold Sph I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
R9507 Taq I from Thermus aquaticus recombinant, expressed in E. coli (Strain that carries a Taq I overproducing plasmid.), Restriction Enzyme Recognition sequence: 5′-T/CGA-3′
Ligation and recutting results: After 2-10-fold Taq I overdigestion of 1 μg λ DNA substrate, results in >95% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
R7260 Xba I from Xanthomonas badrii Restriction Enzyme Recognition sequence: 5′-T/CTAGA-3′
Cutting results: A 2-10-fold Xba I overdigestion of 1 μg Ad-2 DNA substrate results in 100% cutting
Heat inactivation: Up to 15 units of enzyme inactivated at 65 °C for 15 minutes.
R6379 Xho I from Xanthomonas holcicola Restriction Enzyme Recognition sequence: 5′-C/TCGAG-3′
Cutting results: a 2-10-fold Xho I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Inactivated at 65 °C for 15 minutes.